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OfflineFunfarm
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Re: Weird color [Re: Funfarm]
    #27774052 - 05/12/22 01:27 PM (2 years, 8 months ago)

You know the agar starts out super fast but in my opinion with no s.a.b. nor laminar air flow I think opening a bag of grain in a Petri dish to fish out the eggar exposes the mycelium to the open-air contaminants not to mention the three inches of mold I found in one of my windowsill Corners last night as I punch myself in the head only leads to the failed attempts that I've had working with agar in Rye seed so in frustration I thought that by heating the needle and pushing it in through the self-healing port that I might have better luck thank you for the kind words and happy trips

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OfflineB Traven
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Re: Weird color [Re: Funfarm]
    #27774146 - 05/12/22 02:27 PM (2 years, 8 months ago)

No SAB?

You gotta get a SAB.

Start with saran wrap and a cardboard box if you have to, but at least work in still air!


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OfflineFunfarm
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Re: Weird color [Re: B Traven]
    #27774399 - 05/12/22 05:32 PM (2 years, 8 months ago)

On my next paycheck next week I'm getting another clear tote and I found a 4-in hole saw in a thrift shop so I got that going for me. I don't think I'm going to do anymore hobby enthusiasm with fungi until I get the clear tote and some holes in it. Grandma even suggested ordering one of those fancy kits from overseas where you just add water and it grows inside of a Ziploc bag. She said instead of adding the water just take the mycelium that's in the tupperware bowl and throw it in with some rye seed. Kind of a silly way to go about propagation but I guess you got to start somewhere. So I tells Grandma we're going to give it another shot with a still air box I got to figure out how to do this on my own. I'm thinking with the mold in the corner of the window and trying to do this with all the funky nasty in the air that's a huge part of the problem thanks for your responses have a super night

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OfflineFunfarm
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Re: Weird color [Re: Funfarm]
    #27780033 - 05/16/22 09:45 AM (2 years, 7 months ago)

Got the 2 pound rye sitting in the hot water heater closet a nice 70 degrees constant. The bag has white mold all over the bottom of it growing upwards in clumps. I was hoping to get a uline pulse sealer online and do g2g transfer. I wanted to get kitchen cleaning gloves that come up high on the forearm, and transfer the contents of the inoculated 2 pound bag over a quantity of 4 two pound spawn bags. Basically a large handful of colonized grain to each bag in a attempt to keep perpetual propagation of mycellium going. The idea was 5 to 8 pounds colonized rye seed layered with 10 to 20 pounds of prepared manure in a clear tote. Again that was the plan but I can't seem to grow anything but pissy yellow brown agar and that cotton mold ingrained in the rye seed between the inner wall of the poly bag. Grandma keeps laughing at me, and I swear if she keeps mocking me, I'm going to quit buying her beer and vodka. Still air box kung fu is in order, especially if I ever do g2g. Have a glorious magnificent day.

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OfflinePBJ710
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Re: Weird color [Re: Funfarm]
    #27780043 - 05/16/22 10:02 AM (2 years, 7 months ago)

A 4" hole is too small unless you have TINY arms.  A 6-8" hole is much more effective as your arms don't act like plungers pushing air around and they are more comfortable to use.  Many people use an old coffee or bulk food can and heat it up and push it through the plastic for a smooth edged larger hole.

Trust me, the mold in your home is not the issue.  All that matters is that the area inside the SAB with your dishes/bags stays clean.

I highly recommend AGAINST using a spore to grain spawn source for G2G.  You will very likely have some backlevel of contamination in that syringe and going G2G will allow it to expand faster than the mycelium.

IMO if you're not effectively using agar for your grain inoculation, you are fighting the odds for success.  Once you have an actually clean culture everything else falls in place.

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OfflineFunfarm
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Re: Weird color [Re: PBJ710]
    #27780164 - 05/16/22 11:57 AM (2 years, 7 months ago)

How do identify a clean culture? In guessing uniform growth?

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OfflinePBJ710
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Re: Weird color [Re: Funfarm]
    #27780503 - 05/16/22 03:55 PM (2 years, 7 months ago)

Uniform growth centered on your inoculation point without anything else growing on the plate is a pretty safe bet.  It's still possible to have molds get meshed with the mycelium and get uniform growth, but the growth itself looks a bit more disorganized than typical rhizo/tomatose growth.

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OfflineFunfarm
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Re: Weird color [Re: PBJ710]
    #27782868 - 05/18/22 11:18 AM (2 years, 7 months ago)

Right on, and I tell you unfortunately that is not what I see when working with plates, typically uneven with green mold balloon heads, with the agar turning either gray or yellow.

Heres my question, can I take to separate agar plates, inoculate both of them and then sandwich them together spore side to spore sine and allow them to grow together in one taped up plate? I'm thinking the spores won't dry out and it would be embedded and would have plenty of food to grow?  Thoughts?

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OfflinePBJ710
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Re: Weird color [Re: Funfarm]
    #27782891 - 05/18/22 11:42 AM (2 years, 7 months ago)

You're supposed to transfer away from contams, not hide from them.  Your spores are not drying out nor starving for food, but if you agar is too hard it can make it difficult for the mycelium to get started.  Try 7.5-8g agar per 500ml water on the next round and use a sterile loop to streak the spores to the plate.  You need to know where you inoculated the plate so that you can tell where good/bad growth is and then get good growth off those spore plates ASAP.  After you have a transfer from that plate you can let it grow out and start selecting sectors for the most aggressive growth.

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Invisible0001001
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Re: Weird color [Re: PBJ710]
    #27782946 - 05/18/22 12:25 PM (2 years, 7 months ago)

I'd recommend watching some youtube videos to at least get the very basics of working with agar down.

Follow trusted cultivators on here and continue to educate yourself. With the right information & techniques you'll atleast have a chance at success.


0001001

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OfflineFunfarm
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Re: Weird color [Re: 0001001]
    #27783227 - 05/18/22 04:02 PM (2 years, 7 months ago)

Right on it's like plate agar biopsies selectively taking the best genetics from the plate and puzzling it on and further propagation for better results. I'll check out some more YouTube videos for sure and in the meantime I guess I'm going to have to get online and order some more agar I know some guys have suggested making my own but I'm not at that point yet got to still air box so we're doing better guys and girls have glorious day

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OfflinePBJ710
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Re: Weird color [Re: Funfarm]
    #27783297 - 05/18/22 04:54 PM (2 years, 7 months ago)

I made agar in a SAB for a year before I decided I was serious enough into the hobby to get an FFU.  :shrug:  I know making agar sounds super complicated when getting started, but it's really alot easier than it seems.  You can do whats called 'no pour' plates in PP5 containers if you're not confident on your SAB technique pouring them.

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Offlinehazyhorse
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Re: Weird color [Re: Funfarm]
    #27783341 - 05/18/22 05:39 PM (2 years, 7 months ago)

Quote:

Funfarm said:
Got the 2 pound rye sitting in the hot water heater closet a nice 70 degrees constant. The bag has white mold all over the bottom of it growing upwards in clumps. I was hoping to get a uline pulse sealer online and do g2g transfer. I wanted to get kitchen cleaning gloves that come up high on the forearm, and transfer the contents of the inoculated 2 pound bag over a quantity of 4 two pound spawn bags. Basically a large handful of colonized grain to each bag in a attempt to keep perpetual propagation of mycellium going. The idea was 5 to 8 pounds colonized rye seed layered with 10 to 20 pounds of prepared manure in a clear tote. Again that was the plan but I can't seem to grow anything but pissy yellow brown agar and that cotton mold ingrained in the rye seed between the inner wall of the poly bag. Grandma keeps laughing at me, and I swear if she keeps mocking me, I'm going to quit buying her beer and vodka. Still air box kung fu is in order, especially if I ever do g2g. Have a glorious magnificent day.




just wanted to say this will end in failure, even if you wipe iso on the gloves they will be too dirty to keep spawn clean. you need to use a sterile instrument. working with bags inside an SAB is hard & i would recommend moving to jars if you want to go the G2G route. that way you can just dump grains into receiving jars without having to use something to physically remove them from the master jar/bag.

agar is really easy & i recommend you try it out, even if you’re intimidated. it’s very noob friendly & no pours are perfect for SAB work. you’ll get the hang of it really quick. here is a really good beginner agar tek to follow


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OfflineFunfarm
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Re: Weird color [Re: hazyhorse]
    #27783345 - 05/18/22 05:44 PM (2 years, 7 months ago)

Thanks for the link I think you're right it will end in Failure so I'm going to check this no poor a gar out it sounds like something that we can work with good stuff good stuff. If I can say this, if moldy containment was the name of the game, I'd be spot on.

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OfflineFunfarm
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Re: Weird color [Re: Funfarm]
    #27796380 - 05/28/22 12:03 PM (2 years, 7 months ago)

Since I had a hard time with agar plates, I'm going to try a single 5 pound bag of sterilized rye seed and 11 cc's of a single  multiple spore syringe. My grandma is in the kitchen smoking cigs and sipping vodka screaming Failure! Going to see top gun tom cruise taking my breath away f1ying thru the skies with moldy shrooms.

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Offlinehazyhorse
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Re: Weird color [Re: Funfarm]
    #27796388 - 05/28/22 12:17 PM (2 years, 7 months ago)

spore to grain bag is a pretty bad idea, it fails more often than it works. PF tek will probably be your best bet if you want to forgo agar


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OfflineB Traven
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Re: Weird color [Re: hazyhorse]
    #27796408 - 05/28/22 12:38 PM (2 years, 7 months ago)

The great thing about having a hard time with agar is that you can pretty easily just keep trying until you get it right, with minimal time and expense. Going bigger when you can't get agar nailed down is not the right direction.

Do you want one random grow that you get lucky on after who knows how many failures, or do you want to know how to grow more reliably for the future?

Do you want random crapshoot genetics, or do you want some control over what your final harvest will look like?


Seriously, if you're feeling too frustrated to continue with agar right now then just do PF cakes. They'll also give you clone-able and print-able fruits, which you can maybe use to play around with agar further on down the line.


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Offlinehazyhorse
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Re: Weird color [Re: B Traven]
    #27796415 - 05/28/22 12:45 PM (2 years, 7 months ago)

:whathesaid:

agar isn’t hard once you get the hang of it. what failures did you have? maybe we can help trouble shoot


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you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
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new to mushroom cultivation?? read this!!
===================================

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OfflineFunfarm
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Re: Weird color [Re: hazyhorse]
    #27796627 - 05/28/22 04:36 PM (2 years, 7 months ago)

Basically the agar color turned gray one time and then on the second attempt to turn yellow it grew mycelium and it also grew the green mold bubbleheads. I think I probably am getting off base from the original topic of the yellow pissy Brown haggar that I had the second time maybe I should start a new thread concerning multi Spore syringe and rye seed? Thank goodness for the help I get here.  I've had a couple failed attempts at injecting bags to the first time I did four of the one pounders rye seed with multi Spore syringe got nothing and then the second time I tried doing Uncle Ben's rice bags and got absolutely nothing. I thought I was on the right track when I cut up that agar that it turn to Gray and put it in a rye seed bag that was sterile of course opening it up to contaminant and waiting 3 weeks to find absolutely nothing but a bunch of dust so against Grandma's best wishes we're going to try a single five pounder that I ordered and squirt the  whole 11 cc's into it. Grandma told me to order another Spore print and just put it in a jar of popcorn seeds I'll try anything at this point. I think I'm going to go to the library and check out a book on fungus and see about operating with p.f. Tek.

Edited by Funfarm (05/28/22 05:28 PM)

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Offlinehazyhorse
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Re: Weird color [Re: Funfarm]
    #27796652 - 05/28/22 04:47 PM (2 years, 7 months ago)

are you making the agar yourself? or are you buying it premade? if you have a pressure cooker, jars are better to work with inside SABs. bags are really difficult if you aren’t in front of a flowhood


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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