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Blkmycology
Stranger
Registered: 05/27/22
Posts: 2
Last seen: 1 year, 11 months
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Grain-To-Agar DIRECTLY Back-To-Grain (no transfer)
#27795819 - 05/27/22 09:18 PM (1 year, 11 months ago) |
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Hey Guys,
Recently I got a very good genetic ISO’from a friend of mine. I fruited the isolation and I’m very happy with the results. I took some of the grain spawn and put it to agar to keep the mono culture whole. In 5-7 days the mycelium regenerated from the grain on to the agar as expected with no sign of contam. However, I did not perform a agar-to-agar transfer to ensure that my grain-to-agar transfer was 100% contam free. I instead put the few grain-to-agar cultures I had right back to grain. Colonization of the grain has gone very well with still no signs of contam. I am set to spawn a few monotubs with this grain in the next coming days. Should I be worried about this grow seeing that I did not do a agar-to-agar transfer after doing a grain-to-agar transfer? Maybe someone has done this before and can tell me how their experience went over. Did I make huge mistake here? Need feedback*
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LanguidNoodle
little bean



Registered: 09/27/20
Posts: 21
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Re: Grain-To-Agar DIRECTLY Back-To-Grain (no transfer) [Re: Blkmycology]
#27797649 - 05/29/22 12:18 PM (1 year, 11 months ago) |
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If the grain stays clean then you're fine, give em a sniff before spawning if you're really that concerned
Nothing inherently incorrect about the process you've described b/c the logic is all there as far as I can tell and everything along those steps has been clean
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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 10,170
Loc: South Africa
Last seen: 11 hours, 31 minutes
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Re: Grain-To-Agar DIRECTLY Back-To-Grain (no transfer) [Re: LanguidNoodle]
#27798507 - 05/30/22 01:06 AM (1 year, 11 months ago) |
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Give em a sniff?
Wtf kind of a suggestion is that in advanced cultivation?
You won't realy know unless you check on agar again but if your methods are clean then you shouldn't have a problem.
Edited by DERRAYLD (05/30/22 03:16 AM)
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Eclipse3130
Servant of the Fungi



Registered: 10/06/13
Posts: 6,279
Loc: PNW
Last seen: 24 minutes, 23 seconds
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Re: Grain-To-Agar DIRECTLY Back-To-Grain (no transfer) [Re: DERRAYLD]
#27799099 - 05/30/22 02:09 PM (1 year, 11 months ago) |
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You're most likely good to go, the chances of the grain contamming are lessened because it's already colonized, it's not as safe as pure agar but it's pretty close.
Get them on master slants if you want to keep the clone alive
-------------------- "In The Material World One seeks retirement and grows Old In The Magical World One seeks Enlightenment and grows Wiser In The Miraculous World One seeks nothing and grows Lighter As we all tread the Homeward Path we will explore many Realms And one day... we will all Realize that all experiences are Simply Different ways in which The All-That Is Perceives Itself"
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