Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Buy Bali Kratom Powder   Left Coast Kratom Buy Kratom Extract   Myyco.com Isolated Cubensis Liquid Culture For Sale   Bridgetown Botanicals Bridgetown Botanicals   North Spore Bulk Substrate   MagicBag.co All-In-One Bags That Don't Suck   Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Autoflowering Cannabis Seeds   Unfolding Nature Unfolding Nature: Being in the Implicate Order   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work

Jump to first unread post Pages: 1
OfflineCatsLoveHouseMusic
SpeakerFreaker
 User Gallery


Registered: 11/03/18
Posts: 1,085
Last seen: 2 years, 6 months
First time LC, I might’ve went a little overboard. Do I need anything else?
    #27794581 - 05/27/22 01:48 AM (2 years, 7 months ago)

I wanted my first go at LC to work out smoothly, so I did a lot of reading and decided to try a couple different things. I’ll list everything I have so far.

LME, Peptone, H20 for the recipe

- Quality SHIPs, Syringe Filters, 3 way Stopcock valves, PP5 Plastic Lids (trying them out, read silicone gaskets for plastic lids work great.) I also have multiple sizes of SFDs, micropore tape but some say don’t use those.

- I’m on the cusp of ordering a magnet stirrer, only costs like 30$ and seems worth it? I have quart and pint jars, but at also trying out unmodded 500ml media bottles.

I think that’s all, i’m not trying to throw away money but want to have everything one would need and want to experiment a little bit.——— Any suggestions? ———

Extras: Filter Print Post Top
Offlinenektar61S
Heterotroph
Female Unread Journal User Gallery


Registered: 07/04/20
Posts: 3,686
Loc: Hamsterdam
Last seen: 4 hours, 59 minutes
Re: First time LC, I might’ve went a little overboard. Do I need anything else? [Re: CatsLoveHouseMusic]
    #27794600 - 05/27/22 02:38 AM (2 years, 7 months ago)

Why do people use peptone for shroom tek? Curious where you saw that.

It's used in labs specifically to grow bacteria.

If you search it on here, it's mostly on posts from 10 to 20 years ago. I've seen one guy use it for agar on here recently and he ended up with bacteria.

Not saying it's wrong, just wondering where you saw to use it, or if there is a reason to use it?

Some people (including some TCs) use LME alone.

Mushboy does and his tek has some great tips.

I use just 4 grams of dextrose in 100 ml distilled water. It's not the best, but it is so clear (without filtering), it's easy to tell if there is contam.


Edited by nektar61 (05/27/22 06:36 AM)

Extras: Filter Print Post Top
OfflineCatsLoveHouseMusic
SpeakerFreaker
 User Gallery


Registered: 11/03/18
Posts: 1,085
Last seen: 2 years, 6 months
Re: First time LC, I might’ve went a little overboard. Do I need anything else? [Re: nektar61] * 1
    #27794613 - 05/27/22 03:23 AM (2 years, 7 months ago)

I’ve used his shoebox tek for more then enough shoeboxes, so his LC must be fine. His tek is the reason i’m trying unmodded 500ml bottles.

I read about peptone randomly on a post, then started searching for it specifically. I then private messages people who talked about it (mainly reddit but here too,) and many absolutely swear by it. Had one giving me experiments they were doing with/without it and they said it worked very well.

Who knows though, I too get that feeling of “broscience” or whatever you call it… but after talking to people i’m giving it a try, why not. You should too? It costs like 20-30$ on amazon, supposedly that amount will last a long time.

Maby i’ll get shit on, looking forward to finding out

Extras: Filter Print Post Top
Offlinenektar61S
Heterotroph
Female Unread Journal User Gallery


Registered: 07/04/20
Posts: 3,686
Loc: Hamsterdam
Last seen: 4 hours, 59 minutes
Re: First time LC, I might’ve went a little overboard. Do I need anything else? [Re: CatsLoveHouseMusic]
    #27794626 - 05/27/22 03:54 AM (2 years, 7 months ago)

Magenetic stirrer sounds cool. I've thought about getting one. I use a Immersion Hand Blender (also called a Stick Blender. Mine was 22 bucks on Amazon).
https://www.amazon.com/Ovente-Immersion-Multipurpose-HS560B-HS560B-Black/dp/B076MJSRRC/

Magnetic would probably be better for smaller quantities of liquid, or when you need to stir something for a long time.  I use this to do 1600 ml of distilled water when making up 80 plates at a time.

Mushboy's tek just says to shake it up, no mixer is shown.


Quote:

CatsLoveHouseMusic said:
Had one giving me experiments they were doing with/without it and they said it worked very well.




A shroom experiment that isn't using clone culture isn't a real experiment, because of the wild variation possible with multispore culture. VERY few people who do "experiments" are using clone culture.

Quote:

CatsLoveHouseMusic said:
...mainly reddit



I've been called a "gatekeeper" on reddit for recommending someone doing Uncle Ben's tek try agar. haha.

Seen a lot of good stuff downvoted there, and horrible bullshit upvoted. There is some good stuff on there occasionally, not useful tek, mostly just pics of nice final grows.

I saw 4 sickly shrooms growing out of candy get 1000+ upvotes on there.

Reddit is not a good place for tek. The problem on Reddit is if someone gives bullshit tek, no one calls them on it. If someone does there here, they (rightfully) get shouted down.

People on reddit actually complain about that, and say Shroomery is grumpy old people who aren't willing to try anything new.

A lot of posts on reddit are "I'm trying this (wacky thing that won't likely work).

I have NEVER seen the results on reddit. Like "I tried this wacky thing and got this amazing result."

If you do peptone and it works, I'd love to know. Same if it doesn't work. Maybe it's the thing everyone should do. I don't know.

I just think if it outperformed LME alone, more people who grow fat canopy monotubs on here would be doing it and recommending it. A lot of people have certainly tried it on here in the past.

--A grumpy old person

Edited by nektar61 (05/27/22 04:07 AM)

Extras: Filter Print Post Top
OfflineCatsLoveHouseMusic
SpeakerFreaker
 User Gallery


Registered: 11/03/18
Posts: 1,085
Last seen: 2 years, 6 months
Re: First time LC, I might’ve went a little overboard. Do I need anything else? [Re: nektar61] * 1
    #27794650 - 05/27/22 04:16 AM (2 years, 7 months ago)

The first time I heard of it I said the literal same thing, thought it would’ve been talked about more… Whatever. Have a little fun with it.

Bro check this out, little more interesting reddit stuff i’ll throw at you. Tell me it doesn’t sound interesting. Give it a read and get back to me!  Just read the link name

https://www.reddit.com/r/AskDrugNerds/comments/f1iy5c/mushrooms_grown_in_dmt_analog_substrate/?utm_source=share&utm_medium=ios_app&utm_name=iossmf

Extras: Filter Print Post Top
InvisibleWyoMX
I'm a teapot User Gallery
Registered: 07/06/15
Posts: 2,351
Loc: PNW
Re: First time LC, I might’ve went a little overboard. Do I need anything else? [Re: CatsLoveHouseMusic] * 1
    #27794662 - 05/27/22 04:24 AM (2 years, 7 months ago)

This thread talks about something very similar and there's another where I believe pasty talked about it as well. I'll try n find it. https://www.shroomery.org/forums/showflat.php/Number/27458083#27458083

Extras: Filter Print Post Top
Offlinenektar61S
Heterotroph
Female Unread Journal User Gallery


Registered: 07/04/20
Posts: 3,686
Loc: Hamsterdam
Last seen: 4 hours, 59 minutes
Re: First time LC, I might’ve went a little overboard. Do I need anything else? [Re: CatsLoveHouseMusic]
    #27794670 - 05/27/22 04:36 AM (2 years, 7 months ago)

Quote:

CatsLoveHouseMusic said:
The first time I heard of it I said the literal same thing, thought it would’ve been talked about more… Whatever. Have a little fun with it.

Bro check this out, little more interesting reddit stuff i’ll throw at you. Tell me it doesn’t sound interesting. Give it a read and get back to me!  Just read the link name

https://www.reddit.com/r/AskDrugNerds/comments/f1iy5c/mushrooms_grown_in_dmt_analog_substrate/?utm_source=share&utm_medium=ios_app&utm_name=iossmf




I think it sounds like a waste of time, money and chemicals.

Shrooms turn bird food into great drugs. Why would you bother synthesizing or buying expensive DET (which is active in and of itself) to put into shroom sub?

(If you're really going to try tryptamines to try to make stronger cubes, I'd start with something cheap, inactive, and easily available like tryptophan or melatonin before trying something expensive that's already an active drug.)

There are a lot of posts on Shroomery from a decade ago discussing tryptamine additions to increase potency.

Bod has a solid post on here from years ago explaining why it's stupid to add chemicals to substrate, and why people should just grow shrooms.

I'm an OK grower, not a great grower, and in the past year I've grown enough shrooms to last me the rest of my life. I don't understand the fascination with growing super cubes. Even mediocre cubes bend reality pretty far if you eat a few grams of them.

If you want to grow super strong shrooms, grow woodlovers or pans.

Most of the posts about super ways to grow shrooms are by people who haven't yet just grown some normal good shrooms, or haven't done a lot of it.

People who get very good at growing lots of normal shrooms don't usually bother with things like adding DET to the coir. They just grow lots of normal shrooms. Or go into things that are proven and useful like stabilizing phenos.

I would recommend anyone who's going to do something way out off left field master that other stuff first. Dali could paint photo-realistically quite well before he went surrealist. If he couldn't, his surrealism wouldn't have been very good.

I'll say it again: anyone who does "experiments" that aren't done with clones aren't doing science. The data they cull isn't useful.

Edited by nektar61 (05/27/22 04:54 AM)

Extras: Filter Print Post Top
OfflineCatsLoveHouseMusic
SpeakerFreaker
 User Gallery


Registered: 11/03/18
Posts: 1,085
Last seen: 2 years, 6 months
Re: First time LC, I might’ve went a little overboard. Do I need anything else? [Re: nektar61] * 1
    #27794677 - 05/27/22 04:55 AM (2 years, 7 months ago)

Yeah I havnt taken RCs before but I know people like met and the mipt or whatever. I’m learning LC currently, but if it went smooth I see no reason not to try it with MET. Sounds like you needed a lot of the one substance so might not be worth it.

Thought it was interesting

Extras: Filter Print Post Top
InvisibleGoatrider
Rhythm Guitarist
Male


Registered: 04/08/20
Posts: 4,801
Loc: Germany
Trusted Cultivator
Re: First time LC, I might’ve went a little overboard. Do I need anything else? [Re: CatsLoveHouseMusic] * 1
    #27794699 - 05/27/22 05:37 AM (2 years, 7 months ago)

Quote:

CatsLoveHouseMusic said:
Peptone




If you want a cloudy broth from the start, use it.
Malt alone has everything we need for growing mushrooms,
so it´s always just water and lme for me.
Adding some agar-agar to make the broth semi-solid speeds up the whole process.
If you use peptone, make sure you got the right one like soy peptone.
From different protein sources, they have different purposes.
The source can be meat, plants/vegetables...you want the last.
Keep it simple for success :wink:

          :cookiemonster:


--------------------

Extras: Filter Print Post Top
OfflineCatsLoveHouseMusic
SpeakerFreaker
 User Gallery


Registered: 11/03/18
Posts: 1,085
Last seen: 2 years, 6 months
Re: First time LC, I might’ve went a little overboard. Do I need anything else? [Re: Goatrider]
    #27795094 - 05/27/22 12:48 PM (2 years, 7 months ago)

I grow cannabis as well and grow notill, which is super simple and all that so I agree.. I gotta see for myself though! The one thing i’m most “concerned” about is the filter I use and what I stick my needle in.

Trying out SHIP, maby some straight up RTV and the 3 way stopcocks. What’s your thoughts on all that? Have you used stopcocks before?

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: PhytoExtractum Buy Bali Kratom Powder   Left Coast Kratom Buy Kratom Extract   Myyco.com Isolated Cubensis Liquid Culture For Sale   Bridgetown Botanicals Bridgetown Botanicals   North Spore Bulk Substrate   MagicBag.co All-In-One Bags That Don't Suck   Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Autoflowering Cannabis Seeds   Unfolding Nature Unfolding Nature: Being in the Implicate Order   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work


Similar ThreadsPosterViewsRepliesLast post
* Josex' Poke: A different way of doing LC.
( 1 2 3 4 ... 80 81 )
Josex 135,094 1,617 06/30/24 02:25 PM
by Buckdog
* A big pot of LC, revisited CreonAntigone 214 5 05/24/22 02:50 AM
by Bobbins
* Dark syringes and peptone veda_sticks 912 5 11/26/08 08:51 PM
by blood4blood
* Anyone ever used mycological peptone? mungojerry 2,501 3 03/28/07 06:34 AM
by StroFun
* peptone? strangladesh 1,323 4 11/12/06 07:15 PM
by strangladesh
* PEPTONE? rizomorph 1,214 1 12/06/09 04:25 AM
by nonamenomad
* Lc
( 1 2 all )
IGnosticAbhorI 3,404 28 03/28/05 12:15 AM
by IGnosticAbhorI
* Re-Inoculating some WBS jars with LC jtk 587 3 04/22/05 01:46 PM
by NeedMoreSleep

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
267 topic views. 24 members, 121 guests and 24 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.021 seconds spending 0.006 seconds on 12 queries.