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InvisibleHotboxer
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First go at agar, how are the transfers doing? Updated.
    #27788631 - 05/22/22 07:07 PM (2 years, 7 months ago)

The first go at agar was a failure due to overwhelming condensation, however some transfers were salvaged from around 6 o'clock.



10/11/12 were taken from the above pic




These look good to my untrained eye. Puffy cloud right at the center off all, is this tomentose? Standard practice seems to be to transfer once more to ensure cleanliness while some say you can get away with T2 to graain. What should you look for in making that decision?

Below are two transfers taken from a brf cake. The segmentation in the second was a piece of the transfer that broke off enroute.



Edited by Hotboxer (05/26/22 12:05 PM)

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OfflineTheTimelessDon
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Re: First go at agar, how are the transfers doing? [Re: Hotboxer] * 1
    #27788653 - 05/22/22 07:26 PM (2 years, 7 months ago)

Looking great!  From my understanding, as long as the plate is clean then the rest is up to your preference. 

So far, my thoughts if they were mine:  12 is looking like it's going to grow into a niiice circle, and no more transfers needed.  When they look good, it's hard for me to justify cutting it up unless I have a specific mission.  Same with 11... Might be fine as is?  But, since I'd be letting 12 go without any transfers, I would keep 11 as a free agent for now and maybe-maybe not later?  And 10, I would just plan on transferring since I'm not planning on transferring 12, and keeping an eye on 11.  So that would actually cover all scenarios in my mind, and I'd let them off to the races.

Take that for whatever it ends up being worth in the end, lol, and definitely post updates!  Transfers are looking awesome so far.  P.S. Puff in center is normal, just some lost souls looking for some love.

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OfflineTheTimelessDon
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Re: First go at agar, how are the transfers doing? [Re: Hotboxer] * 2
    #27788655 - 05/22/22 07:29 PM (2 years, 7 months ago)

Plate 10 - Will definitely need transferred away from what appears to be a pubic hair on plate.  Lol, sorry I had to add that.  :popcorn:

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InvisibleHotboxer
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Re: First go at agar, how are the transfers doing? [Re: TheTimelessDon]
    #27788696 - 05/22/22 08:02 PM (2 years, 7 months ago)

Thanks for the thoughts, it's a learning process. I think what was going on with 10 is the myc was on the tip of the wedge away from the area that is slow to colonize. Rogue pube for scale.

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OfflineCocaineBuffet
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Re: First go at agar, how are the transfers doing? [Re: Hotboxer]
    #27788701 - 05/22/22 08:06 PM (2 years, 7 months ago)

Keep transfering you are on the right track. I personally let my plates grow out a decent amount before I transfer. Based on your pics I would maybe transfer in a week or 2.

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InvisibleHotboxer
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Re: First go at agar, how are the transfers doing? [Re: CocaineBuffet]
    #27788704 - 05/22/22 08:10 PM (2 years, 7 months ago)

Thanks Cocaine! That was going to be a question, as I thought some transferred at dime or quarter size.

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OfflineRotnpins
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Re: First go at agar, how are the transfers doing? [Re: Hotboxer] * 1
    #27788741 - 05/22/22 08:45 PM (2 years, 7 months ago)

Quote:

matt635 said:
Thanks Cocaine! That was going to be a question, as I thought some transferred at dime or quarter size.




It really  depends.. my understanding is, if you're trying to clean a plate up that has a high probability of contamination, such as clone tissue or a germination plate, it's sometimes best to take a transfer as quickly as possible.. that way you're less likely to take contams to the new plate.. if the plate is already pretty clean, it can be best to wait and see how it grows out..

For example,  I've had a plate that looked pretty clean but wasn't very rizomorphic. I decided to wait a couple  more days and ended up having an easier time finding an organized, ropey, section to transfer from.

Here's the same plate 2 days apart:


I'm still pretty new though, so this as all kinda just speculation on my part...:shrug:

Edited by Rotnpins (05/22/22 08:48 PM)

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InvisibleHotboxer
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Re: First go at agar, how are the transfers doing? [Re: Rotnpins]
    #27789424 - 05/23/22 12:40 PM (2 years, 7 months ago)

Good info rotnpins. Certainly could be beneficial to wait if things look clean. At this point I believe I'm ok to wait and see how things progress.

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InvisibleHotboxer
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Update. Where to go from here? [Re: Hotboxer]
    #27793627 - 05/26/22 11:45 AM (2 years, 7 months ago)

I'm curious what I'm seeing on some of these such as the fluffier/raised portions away from the center. I've seen some saying to take transfers away from these areas? What are they? Those who would advocate for transferring again, what are you seeing/not seeing to make that decision?

Was planning on transferring this one. Somewhere in the 3-5 o'clock range?


This one looks to be turning out nicely. Transfer again or put to grain?


Same with 12. Looks great to my untrained eye. Transfer or put to grain?


How about the BRF transfers? Where should I grab from on here?


Same question on this BRF transfer. Not sure where to grab from.

Edited by Hotboxer (05/26/22 02:56 PM)

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Offlinesan pedro guy
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Re: Update. Where to go from here? [Re: Hotboxer]
    #27794235 - 05/26/22 07:26 PM (2 years, 7 months ago)

Id toss em all on grain after a transfer just as a backup….but thats just me.

The first one is the most sketch but doesn’t look bad to me anyways.

Just send it bro! haha


--------------------
Noob Grow Along 2022

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Offlinenektar61S
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Re: Update. Where to go from here? [Re: Hotboxer]
    #27794262 - 05/26/22 07:38 PM (2 years, 7 months ago)

Quote:

matt635 said:
I'm curious what I'm seeing on some of these such as the fluffier/raised portions away from the center. I've seen some saying to take transfers away from these areas? What are they? Those who would advocate for transferring again, what are you seeing/not seeing to make that decision?

Was planning on transferring this one. Somewhere in the 3-5 o'clock range?


This one looks to be turning out nicely. Transfer again or put to grain?


Same with 12. Looks great to my untrained eye. Transfer or put to grain?


How about the BRF transfers? Where should I grab from on here?


Same question on this BRF transfer. Not sure where to grab from.





I'd send wedges from the edge any of those to grain....except the first one. It's probably fine also, but raised can be contam. Here it's probably just tomotose (fluffy) mycelium, where as some of the other is rhizo (ropey).

Either type of mycleium is good. Some people prefer rhizo because it looks sexy, and because it's easier to tell from mold. Mold is usually fluffy.

Keep some of each plate in case you have to go back a step.

What's your tek for PCing jars, and what's your lid filter situation? Have you done it successfully before or do you need tips?

Welcome,
Kat

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InvisibleHotboxer
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Re: Update. Where to go from here? [Re: san pedro guy]
    #27794392 - 05/26/22 09:13 PM (2 years, 7 months ago)

Ha, that was not the response I expected, but a welcome one!

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InvisibleHotboxer
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Re: Update. Where to go from here? [Re: nektar61] * 1
    #27794397 - 05/26/22 09:17 PM (2 years, 7 months ago)

Thank you Kat! I've done brf cakes successfully. Just had my first go with presto 23qt PC today. Followed Bods grain/PC tek. Lids have synthetic filter discs.

Edited by Hotboxer (05/26/22 09:18 PM)

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OfflineTheTimelessDon
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Re: Update. Where to go from here? [Re: Hotboxer]
    #27794420 - 05/26/22 09:36 PM (2 years, 7 months ago)

Awesome!  I think I was on point with original thoughts before, and these still look great!!

I would do transfers, experiment, and learn some stuff off 10 - but you don't have to.  11, is still up to you - maybe a backup for now?  12, is gorgeous still and I would send that off to grain and hunt down some clone tissue!

You're following the teks and it shows!  Keep it up :popcorn:

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Offlinenektar61S
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Re: Update. Where to go from here? [Re: Hotboxer]
    #27794596 - 05/27/22 02:31 AM (2 years, 7 months ago)

By the way you can put a sealed plate in the fridge and it slows down growing to almost stopped. Then you can transfer from it later. I've done it at 6 months, worked fine. I've got some a year and a half old that still look alive. good when you get a clone you like.

I'm working on slants now. That's for long term storage of cultures.

Sometimes plates dry out in the fridge though, end up looking like fruit leather. haha. depends on if they're sealed with something permeable.

might even still be able to transfer, I haven't tried one of those.

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Offlinenektar61S
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Re: Update. Where to go from here? [Re: Hotboxer]
    #27795678 - 05/27/22 07:51 PM (2 years, 7 months ago)

Quote:

matt635 said:
Thank you Kat! I've done brf cakes successfully. Just had my first go with presto 23qt PC today. Followed Bods grain/PC tek. Lids have synthetic filter discs.





Cool.

Just make sure the lids are on tight. I tighten them with a rubber mat thing that came with some jars. And use that to loosen them after they cool (in front of hood) to inoculate.

I had trouble before I started to tighten them.

Sometimes I have to tap the jar lid on the mat on a table to loosen.

But loose jar lids are a contam vector...when they cool they suck in air through the lid.

It's OK to tighten with filters, they allow pressure to equalize, but filter out contams.

Also, let the PC shoot steam for 10 full minutes before putting on the stopcock. Is loud, but gets the air out, also a vector for contam. 10 min, then put on stop cock, then when it gets to 15 psi, I count 2 hours.  Then let cool to zero pressure, open, put the jars on counter, let them cool to room temp before inoculating.

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InvisibleHotboxer
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Re: Update. Where to go from here? [Re: nektar61]
    #27795896 - 05/27/22 10:55 PM (2 years, 7 months ago)

Quote:

nektar61 said:
Quote:

matt635 said:
Thank you Kat! I've done brf cakes successfully. Just had my first go with presto 23qt PC today. Followed Bods grain/PC tek. Lids have synthetic filter discs.





Cool.

Just make sure the lids are on tight. I tighten them with a rubber mat thing that came with some jars. And use that to loosen them after they cool (in front of hood) to inoculate.

I had trouble before I started to tighten them.

Sometimes I have to tap the jar lid on the mat on a table to loosen.

But loose jar lids are a contam vector...when they cool they suck in air through the lid.

It's OK to tighten with filters, they allow pressure to equalize, but filter out contams.

Also, let the PC shoot steam for 10 full minutes before putting on the stopcock. Is loud, but gets the air out, also a vector for contam. 10 min, then put on stop cock, then when it gets to 15 psi, I count 2 hours.  Then let cool to zero pressure, open, put the jars on counter, let them cool to room temp before inoculating.




Good advice, I'm not sure hand tight is good enough, I'll have to look for one of those grippy things.

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Offlinenektar61S
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Re: Update. Where to go from here? [Re: Hotboxer]
    #27795941 - 05/28/22 12:33 AM (2 years, 7 months ago)

Quote:

matt635 said:
Good advice, I'm not sure hand tight is good enough, I'll have to look for one of those grippy things.




All kinds of colors and styles:
https://www.amazon.com/s?k=jar+gripper

Not everyone does this. I did this to solve a problem I was having about a year ago: I had gotten very good at agar, had clean agar, was transferring, cloning, etc, but was still getting contam jars. Like they'd grow 3 weeks, then stall from invisible bacteria. I worked on my jar lid game and it went away.

After I drop a wedge in the jar, and tighten with the grippy thing, I even wrap Parafilm or cling wrap around the hole, then wrap tape around it. Don't know if that's needed, but I do it, and the other things I mentioned, and I don't get frequent contam jars from clean plates any more.

Edited by nektar61 (05/28/22 01:31 AM)

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