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shroomduck
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Substrate to Substrate transfer?
#27793885 - 05/26/22 03:47 PM (2 years, 7 months ago) |
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I know there are a few threads similar to this, but I haven't seen a solid answer yet. My plan looks like this:
Currently I'm doing spawn -> substrate -> colonize substrate -> growing conditions. Before the last step I would like to take a chunk off the fully colonized substrate, break it up and mix it with fresh, sterile substrate. With this process I could keep on growing without going back to making new spawn.
In other threads I read that the mycelium gets 'tired' and will stop colonizing, but that's something I would like to see for myself. Can someone who has tried this before chime in and let me know why this doesn't work?
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Tight Lunchbox
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Re: Substrate to Substrate transfer? [Re: shroomduck]
#27793894 - 05/26/22 03:52 PM (2 years, 7 months ago) |
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The spawn has all the nutrients, otherwise we'd just throw a chunk of agar in with some substrate and call it good.
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shroomduck
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Quote:
Tight Lunchbox said: The spawn has all the nutrients, otherwise we'd just throw a chunk of agar in with some substrate and call it good.
Substrate has some nutrients, some more than others. Coir, for example, has lots of cellulose and lignin. What if you bought a nutrient-dense substrate from a sponsor vendor, something with manure and other goodies?
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Screwup
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Re: Substrate to Substrate transfer? [Re: shroomduck]
#27793950 - 05/26/22 04:27 PM (2 years, 7 months ago) |
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Quote:
shroomduck said:
Quote:
Tight Lunchbox said: The spawn has all the nutrients, otherwise we'd just throw a chunk of agar in with some substrate and call it good.
Substrate has some nutrients, some more than others. Coir, for example, has lots of cellulose and lignin. What if you bought a nutrient-dense substrate from a sponsor vendor, something with manure and other goodies?
At that point are you not just getting closer to a G2G or BRF2BRF transfer in terms of nutritious spawn?
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Tight Lunchbox
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Re: Substrate to Substrate transfer? [Re: shroomduck] 1
#27793956 - 05/26/22 04:30 PM (2 years, 7 months ago) |
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That seems like a greater hassle than simply making clean spawn and adding it to field capacity coir.
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shroomduck
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It would be so much easier and faster than G2G or making clean spawn! You just have to sterilize the substrate then mix it together, then your growing ~14 days later.
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PBJ710
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Re: Substrate to Substrate transfer? [Re: shroomduck]
#27794138 - 05/26/22 06:16 PM (2 years, 7 months ago) |
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Once you learn how to make clean spawn and what it takes to fruit cubes, you'll understand why this idea is a waste of time.
Keep your cultures growing on agar where you can monitor exactly whats growing then take a transfer to grain when you want to fruit a tub.
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shroomduck
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Re: Substrate to Substrate transfer? [Re: PBJ710]
#27794146 - 05/26/22 06:25 PM (2 years, 7 months ago) |
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You're probably right, but a more detailed explanation would be nice. I'm still going to give it a try, I'll report back the results.
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san pedro guy
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Re: Substrate to Substrate transfer? [Re: shroomduck]
#27794170 - 05/26/22 06:35 PM (2 years, 7 months ago) |
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Once you spawn, the substrate isn’t clean. You might have better luck moving the colonized sub into a sab, cut a piece from the center, then do a transfer…
By the time the first sub is fully colonized you could have more clean spawn ready to go…
where is the time saving?
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PBJ710
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Re: Substrate to Substrate transfer? [Re: san pedro guy]
#27794203 - 05/26/22 06:58 PM (2 years, 7 months ago) |
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If you want consistent healthy growth, the way it works is you isolate healthy growth AWAY from contams on agar and then only expand the 100% axenic cultures. If you expand cultures that are not 100% clean, the contaminants in it will expand at a faster rate and outrun the relatively weak mushroom mycelium. Clean spawn is everything.
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Ashtray161
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Re: Substrate to Substrate transfer? [Re: PBJ710]
#27794319 - 05/26/22 08:16 PM (2 years, 7 months ago) |
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Look up "Grain to Grain" or "G2G" in the search function and you will be able to find a write up on this.
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