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Offlinesyu
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Registered: 04/22/22
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Is it possible that I fucked up my agar mix?
    #27793624 - 05/26/22 11:44 AM (2 years, 7 months ago)

1 week and a half ago I decided to start agar with a tek using, Potato flakes, honey, agar agar and that's it but when doing it I boiled the mix and when I finished I noticed that the honey didn't mixed well in the pan and stuck to my spoon and me being lazy I decided to scoop the honey away lol. But now after inoculated I think it has been 4 - 5 days since and there is nothing on the plates so I wonder if I fucked my mix and the agar turned out to be trash.

The mix was from this tek:



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Offlineholybrown101
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Re: Is it possible that I fucked up my agar mix? [Re: syu]
    #27793630 - 05/26/22 11:48 AM (2 years, 7 months ago)

You should be AIIIEGGHT MEIN. Spores can take awhile. As long as their is nutrition mycelium will eat it. They look alittle on the very nutritious side but shouldnt be a problem. patience ...

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Offlinehazyhorse
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Re: Is it possible that I fucked up my agar mix? [Re: holybrown101] * 1
    #27793749 - 05/26/22 01:57 PM (2 years, 7 months ago)

in the future, i would recommend using teks on here vs youtube. this doesn't seem to be the worst guide, but i don't think using an oven for sterile work is good practice & there is a lot of bad info on youtube & reddit. if you have a PC already & aren't pouring in an SAB, just get some pp5 containers & do no pours.

like holybrown said, it can take awhile for spores to germ. give it a week or two of spores being on the plate before you start to worry.


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Offlinesyu
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Re: Is it possible that I fucked up my agar mix? [Re: hazyhorse]
    #27793792 - 05/26/22 02:28 PM (2 years, 7 months ago)

Yes I figured out that the oven might not be the best trick in the book.
That's the only part that I didn't do his way and instead poured in a SAB.

I will think about pp5 cup looks easier.

But before that I want to finish my condiment cup. Following this I have a question,
Do you think I shouldn't use the condiment cup since the package was already opened?
Or is it still ok just to spray them with 70% iso before the pour.

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Offlinehazyhorse
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Re: Is it possible that I fucked up my agar mix? [Re: syu]
    #27793813 - 05/26/22 02:41 PM (2 years, 7 months ago)

good! the SAB is about the barest minimum you need for proper sterile work. a lot of people pour in SABs with great success as you seem to have, so if you like that keep doing it! but i do prefer no pours personally, it just makes me feel better knowing there isn't a way for me to fuck up the sterilization like i may with pouring & i do like reusing the containers. but again, a lot of people pour in SABs no problem. it's all personal preference

i wouldn't use any cup that had it's inside exposed to air outside an SAB because there would be a chance contaminate spores could fall in. anything that stayed covered should be fine. also, you can't spray anything with 70% iso & expect it to be sterile. it will sanitize but you can't make anything clean enough to do sterile work in. by wiping it at all you introduce a high risk of contams. the 70% iso is more just for cleaning off your hands/stuff inside the SAB to lower the amount of contams on the surface so there's even less chance of accidentally knocking anything in, but it can't sterilize a surface to make it clean enough to pour agar into or anything.


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Offlinesyu
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Re: Is it possible that I fucked up my agar mix? [Re: hazyhorse]
    #27793826 - 05/26/22 02:53 PM (2 years, 7 months ago)

That will help, In that case I will directly switch, you are right reducing
the risk of contamination by directly PC'ing is a game changer.

Do you have a tek in mind that you could share?
At the moment I only have potato flakes, honey and agar agar.

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Offlinehazyhorse
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Re: Is it possible that I fucked up my agar mix? [Re: syu]
    #27793835 - 05/26/22 03:04 PM (2 years, 7 months ago)

yeah that's the peace of mind i prefer with SAB agar. though, if your plates stay clean for a week before use you know your pours were successful. it does make it easier to just PC it wholesale, though.

this agar tek is basically just those ingredients anyways! you can really use any type of container as long as it's glass or PP5, you don't need those exact glad minirounds. i used the twist top ziplock 1/4 pint containers just fine.


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

Extras: Filter Print Post Top
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