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Offlinesyu
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Registered: 04/22/22
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Is that a good spore print?
    #27793443 - 05/26/22 08:57 AM (2 years, 7 months ago)

All in the title


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OfflineThe Tao
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Registered: 09/12/19
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Re: Is that a good spore print? [Re: syu]
    #27793507 - 05/26/22 09:52 AM (2 years, 7 months ago)

It looks nice and dark. Did you make it or buy it? Is it covered with a bit of clear plastic?

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OfflineGarryJarcia
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Registered: 03/09/22
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Re: Is that a good spore print? [Re: The Tao]
    #27793510 - 05/26/22 10:00 AM (2 years, 7 months ago)

Looks good.


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OfflineRotnpins
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Re: Is that a good spore print? [Re: GarryJarcia]
    #27793515 - 05/26/22 10:06 AM (2 years, 7 months ago)

Quote:

GarryJarcia said:
Looks good.





:whathesaid: :thumbup:

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Offlinesyu
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Re: Is that a good spore print? [Re: The Tao]
    #27793522 - 05/26/22 10:13 AM (2 years, 7 months ago)

I made it myself, my first try is there any sanitary procedure or doing agar will clean it

Edited by syu (05/26/22 10:14 AM)

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InvisibleGuerrilla
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Registered: 01/30/21
Posts: 3,179
Loc: United Kingdom
Re: Is that a good spore print? [Re: Rotnpins]
    #27793523 - 05/26/22 10:14 AM (2 years, 7 months ago)

Looks good, but is this in a sterile environment? If this is in open air then that print is fucked already.


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Being pissed on does not make you a real man.

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OfflineMrBlueshrooms
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Re: Is that a good spore print? [Re: Guerrilla]
    #27793533 - 05/26/22 10:24 AM (2 years, 7 months ago)

Also make sure you dry your prints before storing them. This will greatly increase their shelf life.

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Offlinesyu
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Re: Is that a good spore print? [Re: MrBlueshrooms]
    #27793541 - 05/26/22 10:34 AM (2 years, 7 months ago)

Quote:

Guerrilla said:
Looks good, but is this in a sterile environment? If this is in open air then that print is fucked already.




My procedure was to:
Cut the mushroom top with gloves and scissor sanitized then get a 8qt shoebox wipe it down with 70% iso
get my foil cut it in half put it down in the shoebox with the mushroom cap close it,
4h later take switch the foil for a new one and waited 24h.


Quote:

MrBlueshrooms said:
Also make sure you dry your prints before storing them. This will greatly increase their shelf life.




I might have fucked up there, just reopened the print and
put it back in the shoebox for drying might affect his contamination rate
but it't just a trial.

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OfflineThe Tao
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Re: Is that a good spore print? [Re: syu]
    #27793572 - 05/26/22 10:54 AM (2 years, 7 months ago)

I do 1st  print for 6-12  hours then 2nd print 12-24 hours. I do let prints dry for 24 hours before sealing them up to allow moisture to evaporate.
What did the 1st print look like?
Having it in open air isn’t a good idea but it isn’t ruined. No spore print is ever considered to be clean.

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Offlinesyu
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Re: Is that a good spore print? [Re: The Tao]
    #27793595 - 05/26/22 11:15 AM (2 years, 7 months ago)

It looked like that same thing but faintly like there was less. And it wasn't open air I closed the lid.

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OfflineThe Tao
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Re: Is that a good spore print? [Re: syu]
    #27793608 - 05/26/22 11:28 AM (2 years, 7 months ago)

2nd print is considered cleaner but still not clean. I keep both prints and label them 1st or 2nd.
Why is the foil dimpled? I thought it was moisture the first time I looked at it.

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Offlinesyu
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Re: Is that a good spore print? [Re: The Tao]
    #27793617 - 05/26/22 11:36 AM (2 years, 7 months ago)

No idea honestly it came like that and was the only foil I had. I hope this print is not full of contamination and I will be able to use it lol.

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OfflineThe Tao
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Re: Is that a good spore print? [Re: syu]
    #27793621 - 05/26/22 11:40 AM (2 years, 7 months ago)

Take it to agar or BRF. Take it to grains at your own peril.
How did you end up with a mushroom to take a spore print from in the first place?

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Offlinesyu
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Re: Is that a good spore print? [Re: The Tao]
    #27793636 - 05/26/22 11:53 AM (2 years, 7 months ago)

I did MSS to grain, using brown rice.

But now I want to switch to agar with oats, bought everything but
my agar doesn't seem to work. Or I'm being impatient but really excited
since I inoculated 6 agar plates and still have 8 for transfer.

Another post for my agar if you wanna see it.
https://www.shroomery.org/forums/showflat.php/Number/27793624

And I still got around 2oz using mss to brown rice.

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OfflineRotnpins
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Re: Is that a good spore print? [Re: The Tao]
    #27793654 - 05/26/22 12:19 PM (2 years, 7 months ago)

Quote:

The Tao said:
2nd print is considered cleaner but still not clean. I keep both prints and label them 1st or 2nd.
Why is the foil dimpled? I thought it was moisture the first time I looked at it.




I think its from a box of pre-cut foil sheets.. I have a box with foil that looks just like that.

Spores can take a while to germinate... first time I did spore solution to agar, I waited like a week and thought it failed, but I kept the plates sitting on my shelf.. I decided to look at them after about 2 more weeks and a couple of them had growth.... when doing germination plates, especially from spore solution and not a print or swab, I usually do a few extra plates just to make sure I get a couple options to work with.

Edited by Rotnpins (05/26/22 12:25 PM)

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Offlinesyu
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Re: Is that a good spore print? [Re: Rotnpins]
    #27793663 - 05/26/22 12:36 PM (2 years, 7 months ago)

That's good to hear, I was just impatient.

I'm just skeptical because all agar that I tried failed miserably
probably due to lack of technique, I bought some pre-poured agar.
And couldn't get a single one to germinate. So I decided
to do my oned and that's where I am now.

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OfflineRotnpins
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Re: Is that a good spore print? [Re: syu]
    #27793672 - 05/26/22 12:42 PM (2 years, 7 months ago)

I used pre-mades for a little while. If I was using them as a germ plate, and they didn't germinate,  I would re-use the same plate and give it a second try... I was a lot more conservative with the pre made plates. I wish you the best of luck with doing your own plates.. it's definitely a skill set that opens a lot of doors in this hobby.

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