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Offlinemake air go wooo
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Registered: 12/02/21
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Spore syringe to agar.
    #27793404 - 05/26/22 08:07 AM (2 years, 7 months ago)

When I was spreading the mushy seeds onto my agar plates with a inoculation loop, I would heat up the loop, cool it by dipping in agar, then dip the loop into the syringe liquid.  Many times I would hear a hissing sound when dipping the loop into the syringe liquid.  Did this kill any good mushy seeds that I was spreading on the agar plates?

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OfflineScrewup
Googles your dumb questions
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Registered: 01/27/22
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Re: Spore syringe to agar. [Re: make air go wooo]
    #27793479 - 05/26/22 09:22 AM (2 years, 7 months ago)

If you heard hissing that loop wasn’t as cool as it probably should’ve been. Some spores may have died or all of them. You’ll only know after time.

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OfflineDERRAYLD
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Registered: 05/13/02
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Re: Spore syringe to agar. [Re: Screwup]
    #27793488 - 05/26/22 09:29 AM (2 years, 7 months ago)

Considering you're most likely streaking thousands of spores... you're okay.

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