|
zilker
No Entitlements Here


Registered: 05/25/22
Posts: 66
Loc: Texas
Last seen: 4 months, 24 days
|
Newbie Needs Help - Monotub with Forced AE
#27791959 - 05/25/22 08:20 AM (2 years, 7 months ago) |
|
|
This is my second attempt. The first of which ended with trich...such a depressing event that was. However, I built a pop-up clean room, cleaned everything with 10% bleach and let dry, then 70% ISO and let dry.
I followed a video online and layered substrate and grain spawn...3 layers of each. I used a product called MGP Plus to help reduce the risk of trich and also promote growth. I layered the tub on May 16 and introduced the light on May 20 (was fully re-colonized due to growth additive). I have it on a 10 hour light cycle (on 10, off 14) and I'm pushing in air for 30 minutes at a time with 60 minutes in between cycles. The air is being pushed in from an air compressor and goes through two 0.22um filters. The volume of air going in is low...blow up a balloon in 90 seconds or so.
The first picture was taken on May 21. The second & third pictures were taken today, May 25...same picture, different lighting effect. I'm not really sure what I'm dealing with here, but I have a feeling it isn't progressing the way it should.
Can someone please give some input on whether or not this looks right and if not, provide some pointers on how to fix?


|
Goatrider
Rhythm Guitarist



Registered: 04/08/20
Posts: 4,801
Loc: Germany
|
Re: Newbie Needs Help - Monotub with Forced AE [Re: zilker]
#27793165 - 05/26/22 01:03 AM (2 years, 7 months ago) |
|
|
TrichEVICT... I never used this stuff, but believe me, if you don´t wanna see trich before a first flush, just make clean spawn. It´s not a bad idea to be clean, but you don´t need to overthink it to grow mushrooms. A SAB is all you need to do your crucial works in. By the time you open your fully colonized healthy jars, sterility needs are gone.
My advise to you: Forget about the videos, and stick to one of the teks on the board. Agar, grains of your choice and coir. Agar for making a healthy culture, grains for nutrients to inoculate, and coir to bulk and for spending moisture. No more additives needed. The more you put in, the more confusion you´ll have if it doesn´t work.
Forget about the air pump. Really not needed for cubensis. If unmodified, just flip the lid for passive air exchange or try this. Also no need for a direct lighting cycle while colonizing. Some ambient will be enough. Start the cycle 12/12 by the time you see primordia.
So i don´t know if you´ll get something out there. There metabolites, so bacterial activity. Just let it fight, and leave it alone. Provide some little passive air exchange and wait for a surprise. In the meantime do some reseach on the board. Look here, pick a tek of your needs and start real cultivation 
--------------------
|
DERRAYLD
Constructus


Registered: 05/13/02
Posts: 12,105
Loc: South Africa
Last seen: 8 minutes, 14 seconds
|
Re: Newbie Needs Help - Monotub with Forced AE [Re: Goatrider] 1
#27793172 - 05/26/22 01:20 AM (2 years, 7 months ago) |
|
|
Long story detailing how you created a clean room but no mention of how you go about creating clean spawn, 100% that's where your problems are coming from. Trichevict won't clean your dirty spawn.
|
zilker
No Entitlements Here


Registered: 05/25/22
Posts: 66
Loc: Texas
Last seen: 4 months, 24 days
|
Re: Newbie Needs Help - Monotub with Forced AE [Re: DERRAYLD]
#27793290 - 05/26/22 06:15 AM (2 years, 7 months ago) |
|
|
Liquid culture from Spore Works. Grain spawn bags and substrate from Midwest. Could be dirty spawn or bad culture, but I've read good reviews about the suppliers, so I don't know.
Edited by zilker (05/26/22 06:37 AM)
|
LotKid
Never.Trust.A.Prankster



Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
|
Re: Newbie Needs Help - Monotub with Forced AE [Re: zilker] 1
#27793324 - 05/26/22 06:43 AM (2 years, 7 months ago) |
|
|
You sure it was LC? Only LC i know of that SW sells are gourmet and medicinal ones. Any cubes only come in prints and syringes and are for microscopy only.
If you attempted this with a spore syringe, which i belive is the case. Yes, SW is reputable. But, dosnt matter how reputable you are or how nice your lab is.... spore syringes are never gonna be 100% clean.
You, my friend, have dirty spawn and an over complicated setup. Its very easy to over think this hobby. Its very easy to love your project to death. But, its not hard to grow cubes.
|
zilker
No Entitlements Here


Registered: 05/25/22
Posts: 66
Loc: Texas
Last seen: 4 months, 24 days
|
Re: Newbie Needs Help - Monotub with Forced AE [Re: LotKid]
#27793392 - 05/26/22 07:59 AM (2 years, 7 months ago) |
|
|
Yes, it was a spore syringe, not LC...my foul, coach! The strain is APE. I actually read a post this morning specific to APE and my takeaway from it was that "blobs" are somewhat normal if the casing layer is too thin. I don't know if I have blobs or stroma or bacteria or some other F'ing issue (not short for Fruiting!), but believe me when I say I've read, and read, and read, and read. However, I do find there to be quite a lot of conflicting information.
Over complicated setup...guilty. For reasons unsaid, and to further prove your statement correct, my mono is actually inside a larger tub to conceal my hobby. That's why I'm using forced air, it doesn't get proper AE without being forced into the mono.
A middle-aged, modern day wise man once said "Nothing proves difficult once learned. It's only the learning curve that proves difficult". That's exactly where I am...at the bottom of the learning curve, looking up.
Edited by zilker (05/27/22 03:09 PM)
|
DERRAYLD
Constructus


Registered: 05/13/02
Posts: 12,105
Loc: South Africa
Last seen: 8 minutes, 14 seconds
|
Re: Newbie Needs Help - Monotub with Forced AE [Re: zilker] 1
#27793419 - 05/26/22 08:31 AM (2 years, 7 months ago) |
|
|
I'm also noticing a distinct lack of a SAB mentioned here. You setup this pop-up room but do your grain work in there which is almost definitely part of your problem as you are most likely introducing a crap load of your own contamination just moving in the space.
|
KeyMaker
Urban Wizard

Registered: 01/03/22
Posts: 561
Loc: in a van down by the river
|
Re: Newbie Needs Help - Monotub with Forced AE [Re: DERRAYLD]
#27793440 - 05/26/22 08:52 AM (2 years, 7 months ago) |
|
|
I've always used 6 different 1 1/8" holes drilled in my tubs with Polyfil, never had an AE problem. I usually don't open the lids until I see pins, even then, after that I just open it to pick mature fruit. It sounds like you did a great job on your clean room. Now put that energy into an SAB and be as meticulous as you were when you cleaned the room. Make some agar, that changed everything for me.
|
zilker
No Entitlements Here


Registered: 05/25/22
Posts: 66
Loc: Texas
Last seen: 4 months, 24 days
|
Re: Newbie Needs Help - Monotub with Forced AE [Re: Goatrider]
#27795010 - 05/27/22 11:31 AM (2 years, 7 months ago) |
|
|
Taking your advice on the teks. I honestly hate to f'n read as I'm much more of a movie kinda guy, but I get it. I couldn't help but laugh at myself when reading the following, b/c after all replies (thank you ALL very much, BTW), it appears I got kicked in the nuts a second time:
you bought a grow kit  Take a look here and see if there's any way to salvage it. Chances are you just gave your money to someone who shipped you a box full of reminders that you just got kicked in the nuts. Don't fret though for less money then you spent on the kit you can get going the right way.
I do have a quite few questions about the tasks completed in SABs, but I'll first try to located the answers myself.
Again, thanks to all who replied to my thread.
|
LotKid
Never.Trust.A.Prankster



Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
|
Re: Newbie Needs Help - Monotub with Forced AE [Re: zilker] 1
#27796141 - 05/28/22 07:36 AM (2 years, 7 months ago) |
|
|
If you hate reading then this hobby might not be for you. Because there's crazy amounts of reading invloved.
|
|