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Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures

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Offlinemake air go wooo
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Registered: 12/02/21
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Agar condensation.
    #27790392 - 05/24/22 08:10 AM (2 years, 7 months ago)

Should I wait until I see no condensation on the plates before I streak them with a syringe?
I have the SAB holes covered with a towel.

Edited by make air go wooo (05/24/22 08:16 AM)

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OfflinePBJ710
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Re: Agar condensation. [Re: make air go wooo]
    #27790414 - 05/24/22 08:29 AM (2 years, 7 months ago)

They're fine to use like that, just not as easy to see through.  After you seal them back up, some of the moisture will be absorbed by the agar - you just don't want any moisture to collect and pool on the surface of the plates.  If the condensation is really bad, you can turn the plate sideways and pour out the moisture from the gap under the lid while the dish stays closed.  Try to pour your plates closer to 118-120f next time and stack your plates higher while cooling and usually only the top couple plates will have any condensation. 

I get why you closed up the SAB to help keep them clean, but I'm curious how you expect the air in the SAB to dry them if you have no air exchange to bring fresh dry air into the SAB?

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Offlinemake air go wooo
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Re: Agar condensation. [Re: PBJ710]
    #27790447 - 05/24/22 09:01 AM (2 years, 7 months ago)

Very good reply

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OfflineScrewup
Googles your dumb questions
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Registered: 01/27/22
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Re: Agar condensation. [Re: PBJ710]
    #27790498 - 05/24/22 10:03 AM (2 years, 7 months ago)

So does the stacking the plates after you’ve cooled them also help with condensation? I saw on one of teks that if you have condensation to put warm water in a flat item on top of the dish. This is the same thing?

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OfflinePBJ710
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Re: Agar condensation. [Re: Screwup]
    #27790515 - 05/24/22 10:20 AM (2 years, 7 months ago)

The stacking / warm cup thing only really works well while plates are still cooling.  Having warm plates above prevents the temperature differential from occurring as much so the moisture tends to remain in the agar instead of condensing out along the cooler top of the plate.  Once it cools and the water condenses, adding the warm cup above doesn't do near as much good since the cool agar will not readily re-absorb the moisture.  It won't hurt anything to try it though.

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