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OfflineAlpineHighs
Thrill Shrieker

Registered: 04/22/20
Posts: 87
Loc: Montucky
Last seen: 2 years, 5 months
"Bad" smelling agar plate - start over?
    #27789081 - 05/23/22 06:16 AM (2 years, 7 months ago)

I just inoculated 8 jars with plates I assumed were clean. They all look like this...



When I removed the plates from the SAB and smelled them...I was thoroughly disappointed. They smell pretty damn bad. Assuming bacterial.

Is there any point in seeing if the jars even colonize? Or should I toss all this shit and start over

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OfflineTheTimelessDon
The Don


Registered: 03/19/22
Posts: 194
Last seen: 4 months, 30 days
Re: "Bad" smelling agar plate - start over? [Re: AlpineHighs]
    #27789084 - 05/23/22 06:26 AM (2 years, 7 months ago)

I'm loving all your triangle cuts btw...

What kind of smell?  I'm definitely not able to help with smell identification, but the plate looks great.  Maybe something with the agar recipe or ingredients used?  I believe malt extract can give off a sugary smell...

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OfflineDERRAYLD
Constructus
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Registered: 05/13/02
Posts: 12,109
Loc: South Africa
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Re: "Bad" smelling agar plate - start over? [Re: AlpineHighs]
    #27789085 - 05/23/22 06:28 AM (2 years, 7 months ago)

What was your agar recipe?
Nothing stands out from that plate, bacteria generally smells before mold but I don't see any bacteria.

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OfflineAlpineHighs
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Registered: 04/22/20
Posts: 87
Loc: Montucky
Last seen: 2 years, 5 months
Re: "Bad" smelling agar plate - start over? [Re: DERRAYLD]
    #27789094 - 05/23/22 06:38 AM (2 years, 7 months ago)

Smells musty and dirty. Not quite sour, but it's terrible. Not the faintest hint of mushroom smell. Maybe there's a white mold growing with the myc?

10g agar - 9g LME - 500ml H20

Bummed. I'm just staring at my grow space trying not to concede to a month of wasted time. Ha.

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OfflineDERRAYLD
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Re: "Bad" smelling agar plate - start over? [Re: AlpineHighs] * 1
    #27789098 - 05/23/22 06:43 AM (2 years, 7 months ago)

The plate says T7, did you transfer 7 times to get that plate?

I think you need to work on identifying clean mycelium if this is a T7, sorry to be blunt.

Clean  plates have a slight agar smell with a hint of malt sweetness.

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OfflineAlpineHighs
Thrill Shrieker

Registered: 04/22/20
Posts: 87
Loc: Montucky
Last seen: 2 years, 5 months
Re: "Bad" smelling agar plate - start over? [Re: DERRAYLD]
    #27789104 - 05/23/22 06:48 AM (2 years, 7 months ago)

Yeah T7. I had colonized plates in ziplocks in my fridge for like 5 months. Was gunna toss them and start over with new spores, but I opened one and it smelled great. So I did transfers. Now they smell awful.

Could be a very contaminated older syringe. I inoculated a bunch of plates, and the germination was weak. Opened a few germination transfer plates, and they stink too.

Back to square one I guess.

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