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InvisibleQM33
(NOT A PUPPET!) ❤❤❤❤❤
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Registered: 04/09/20
Posts: 4,739
Loc: Oregon
Genetic Transformation of Basidiomycetes assisted with Calcium Chloride (Ez Fusion Kit by MAGIC MYCO)
    #27789051 - 05/23/22 05:20 AM (1 year, 8 months ago)

Scam?!

Seriously you think your smart? Let's make since of this.

I talked to him. And he's using Calc2, calcium chloride essentially to either force anastomosis, or pull plasmid from the cell and have it drawn into another.

I hope theirs someone who can talk to me about this. I have a few papers I could provide that I had to find myself.


He actually also pretty much told me it hasn't been verified to work and people are encouraged to join the experiment for future results on Patreon lol!?

Relative info


https://www.sciencedirect.com/science/article/abs/pii/037811198390238X


https://www.mybiosource.com/learn/testing-procedures/cacl2-transformation-technique/

Probably most straight to the point.
https://en.m.wikipedia.org/wiki/Calcium_chloride_transformation

Also I asked for instructions which I should be able to post if necessary but it does involve a cold shock and a heat pad or something along those lines, instructed from Magic Myco.


Okay so here's the delima.. I'm pretty sure it only would work with prokaryotes, not eukaryotes, mainly because the cell wall is protecting them.





PLEASE PROVE ME WRONG.

OR PLEASE SUPPORT ME IN WEEDING OUT ALL THESE FAKE MONEY HUNGRY, EVIL, PROFITEERS.

How many sells and no results?

Sounds like Dr myc again...


Good thing I got a buddy deep into experiments and preparing to present all this to the world.



Halllllppp




*Bump, title change.


--------------------
OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum...
There are known knowns, there are known unknowns,
          there are also unknown unknowns.
With great privilege comes great responsibility.

Quantom Qups PROOF AND Soft Drops
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Edited by QM33 (06/01/22 05:44 PM)


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InvisibleQM33
(NOT A PUPPET!) ❤❤❤❤❤
I'm a teapot User Gallery


Registered: 04/09/20
Posts: 4,739
Loc: Oregon
Re: Ez Fusion Kit by MAGIC MYCO [Re: QM33]
    #27789063 - 05/23/22 05:39 AM (1 year, 8 months ago)

Also I could see where the confusion arise also because non only bacteria are prokaryotic, but also as an example, a subphylum as ascomycetes known as
https://en.m.wikipedia.org/wiki/Saccharomycotina




Also I was talking to someone else and they were talking about PEG8000 in conjunction with calc2. Which also seems to make since in a vague, not necessarily related since.

https://www.frontiersin.org/articles/10.3389/fmicb.2019.02675/full

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6883257/

But I also know magic myco was experimenting with peg8000 for a while, and he also wa she one guiding this other person doing similar things.

So as far as I'm concerned it's all on Magicmyco. And again he has provided nothing but admission that this hasn't been proven successful




Confused yet? Me too.





TLDR,

can you use calcium chloride ot PEG to force DNA inheretence ,in any form , in Basidiomycetes/ Eukaryotic or dikaryotic fungi?


--------------------
OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum...
There are known knowns, there are known unknowns,
          there are also unknown unknowns.
With great privilege comes great responsibility.

Quantom Qups PROOF AND Soft Drops
Turn your Swab to a Syringe and Syringe to Multiple Syringes!
No Pours (QuantomStyal)
Magic Fruit Leather
DMT for IandI


Extras: Filter Print Post Top
InvisibleQM33
(NOT A PUPPET!) ❤❤❤❤❤
I'm a teapot User Gallery


Registered: 04/09/20
Posts: 4,739
Loc: Oregon
Genetic Transformation of Basidiomycetes assisted with Calcium Chloride (Ez Fusion Kit by MAGIC MYCO) [Re: QM33]
    #27801767 - 06/01/22 05:49 PM (1 year, 7 months ago)

Basically I think it works for prokaryotes because the calcium chloride can penetrate directly through the organism and effect the DNA.

Whereas in a eukaryotic or Mon or dikaryotic cell, it still has the cell protecting the DNA contained in the nuclei, and therefore cannot be effected by the calcium chloride.


Come on, Alan? Bod? Eclipse? Anyone?


Can anyone help me find one paper that shows this would work for eukaryotes?!

I have Doma telling me it's calcium chloride and the instructions are nearly identical to what the wiki article and others I've seen describe.



Bump/title change


--------------------
OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum...
There are known knowns, there are known unknowns,
          there are also unknown unknowns.
With great privilege comes great responsibility.

Quantom Qups PROOF AND Soft Drops
Turn your Swab to a Syringe and Syringe to Multiple Syringes!
No Pours (QuantomStyal)
Magic Fruit Leather
DMT for IandI


Edited by QM33 (06/01/22 05:49 PM)


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OfflineMuad.Dweeb
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Registered: 04/13/17
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Last seen: 5 months, 7 days
Re: Genetic Transformation of Basidiomycetes assisted with Calcium Chloride (Ez Fusion Kit by MAGIC MYCO) [Re: QM33]
    #27939935 - 09/08/22 07:32 AM (1 year, 4 months ago)

Ca+ cations are used in cellular orchestration, but I don't think it's involved in anything that will dedikaryotize or strip the cells off a culture.

Besides, it's completely unneeded to breed cultures. All you need is spore.


--------------------
A beginning is the time for taking the most delicate care that the balances are correct. This every sister of the Bene Gesserit knows.



How to Breed like the Bene Gesserit
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