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The Holy Reality
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Registered: 02/19/22
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First attempt at Agar was an epic failure! 1
#27788418 - 05/22/22 04:09 PM (2 years, 7 months ago) |
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Made a Spore print from my first GT grow & transferred to some pasty plates. I just did the glass over the cap on tinfoil thing, no SAB cause I was gonna do transfers so wasn't really worried about contams. I did use a SAB and sterile techs when transferring the spores though. It's been 5 days & this is what grew on 5 of 7 plates. When I first saw it I thought maybe the good stuff might outgrow the bad so I could get a small piece to transfer, but not anymore. I'm thinking these are trash. The green I'm guessing is penicillium but the yellow I'm not to sure. Any ideas? Thanks in advance

-------------------- Don't tell & don't sell
Edited by The Holy Reality (05/22/22 04:33 PM)
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jack_straw2208
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Registered: 02/12/07
Posts: 3,141
Loc: Earth
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Epic failure or two slides of success?
-------------------- If you canβt tell what you desperately need, itβs probably sleep.
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Infinite Monkeys

Registered: 09/01/21
Posts: 276
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Re: First attempt at Agar was an epic failure! [Re: jack_straw2208] 2
#27788460 - 05/22/22 04:48 PM (2 years, 7 months ago) |
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Try not to see this as a failure because it was not one. This is an opportunity to learn. We all began at the same point.
Roger rabbit's signature has a wonderful phrase.
Quote:
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
When it comes to mush cult, I prefer to go through each step and try to understand out what went wrong and what I can do differently next time.
First and foremost, the problem could be the printing method you used. You want to be sterile while taking the print, regardless of whether you plan to clean it up with agar.
What method did you use to create the agar? Do you have any remaining agar plates?
I like to leave a couple of agar plates uninoculated from each batch. If contams are found on the unused plates, this indicates that the agar pouring/creation procedure is one of the sources of the problem.
What method did you use to put the spores on the plate?
You should use an inoculation loop or a sterile swab to streak a very small amount of spores to the agar,
It makes logical to streak the fewest amount of spores to each agar plant if we presume the spore print is infected. This will help to reduce the quantity of potential contamination on each dish. This also allows the spores more time to germinate and produce mycelium before any possible contamination occurs.
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The Holy Reality
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Registered: 02/19/22
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Re: First attempt at Agar was an epic failure! [Re: Infinite Monkeys] 2
#27788533 - 05/22/22 05:48 PM (2 years, 7 months ago) |
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Yeah I'm not giving up for sure. I'm positive the print tech. I used contributed to it. The plates were about two weeks old, just had them sitting in the fridge & I used pastywhites easy agar tek. Left them wrapped up from the PC until I used them. I did use an inoculation loop and flamed it every time, SAB, A/C turned off, Iso wiped everything. I think the print is the main culprit. I should have been more sterile when taking it. I underestimated the contaminants strength for sure. I'm gonna make up some more Agar tomorrow and give it another go. I got a PES amazonian print from a site trusted vendor I'm gonna try & I ain't giving up on my GT print either. Success is just around the corner!
-------------------- Don't tell & don't sell
Edited by The Holy Reality (05/22/22 05:50 PM)
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ImJonas
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Registered: 05/21/22
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I tried working with agar. At this point I think I'm growing some very well isolated mold.
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Tile
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The Holy Reality
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Registered: 02/19/22
Posts: 154
Loc: Around the corner
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Re: First attempt at Agar was an epic failure! [Re: Tile]
#27820663 - 06/15/22 11:05 AM (2 years, 6 months ago) |
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Sorry for bumping my own thread but just wanted to say I spoke to soon. Seven of my GT print pastyplates had to be thrown out due to hella contam but the last one, which grew very slow I was able to get four rice size transfers to new plates. The plate in the first pic looks a little sketchy to me. Growth came from two different sides. so I'm gonna do a second transfer today. The other two were very uniform in their growth, no obvious contam that I could see so I tiger dropped 2.0'd them to WBS after about 90% plate coverage. Sorry for the condensation and pic quality but I'm not quite confident in my abilities just yet to pour Agar, but I get a little more confident every day.



-------------------- Don't tell & don't sell
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KROM
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