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OfflineBcuzz
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stacking Monotub is causing problems
    #27780125 - 05/16/22 11:27 AM (2 years, 7 months ago)

Good day,
I have a big problem and it is about the stacking of the monotub with me. The upper is totally fogged as you can see and the surface is barely colonized, the lower is dry and totally overgrown.
It is the same variety, it was planted on the same day under the same conditions, now I think that the stacking of the boxes has something to do with it. Can someone with much more experience tell me something about it?







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OfflineHindsight
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Re: stacking Monotub is causing problems [Re: Bcuzz] * 2
    #27780131 - 05/16/22 11:33 AM (2 years, 7 months ago)

The mycelium generates heat and heat rises. Weight on the lids can decrease airflow though this is more of an issue in unmod tubs - I see you have holes in the upper-sides of yours so that shouldn't impact you. The heat generated is enough to change substrate temps - the lowest sub in the stack will be coldest, highest one will be the most warm. I would be surprised if this caused colonization problems though, unless your room temp is already on the upper end of the ideal temperature range and the rising sub heat is pushing the upper tubs past that range. The fogging is from the extra differential between inner tub temp and room temp - condensation forms in this situation.

Measure your room temp with a probe thermometer then measure the sub temp of the upper sub with that same probe and report back.

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Invisible0001001
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Re: stacking Monotub is causing problems [Re: Hindsight] * 2
    #27780145 - 05/16/22 11:44 AM (2 years, 7 months ago)

I don't know if its just the picture but the very last one you posted looks hella contaminated.

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InvisibleBaba Yaga
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Re: stacking Monotub is causing problems [Re: 0001001] * 1
    #27780229 - 05/16/22 12:49 PM (2 years, 7 months ago)

^^^ what they said.

my guess is that your problem is spawn related.

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InvisibleMysticMycologist
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Re: stacking Monotub is causing problems [Re: Baba Yaga]
    #27780241 - 05/16/22 01:00 PM (2 years, 7 months ago)

How did your spawn look? How did you make it?


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OfflineBcuzz
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Re: stacking Monotub is causing problems [Re: MysticMycologist]
    #27780299 - 05/16/22 01:44 PM (2 years, 7 months ago)

first, thank you for the feedback,
my mycelium is not infested with mold. It looks like it but everything is ok. My spawn is made of rye berries and it is with coco,vermiculite and gyps.

right now the soomer is really bothering me in the apartment, i have a thermostat here that tells me in my chamber it is 25 °C (77F) with 49% humidity.

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Invisibleseldom seen
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Re: stacking Monotub is causing problems [Re: Bcuzz] * 1
    #27780306 - 05/16/22 01:51 PM (2 years, 7 months ago)

You have two different organisms in that bottom pic.


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InvisibleMysticMycologist
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Re: stacking Monotub is causing problems [Re: Bcuzz]
    #27780314 - 05/16/22 01:59 PM (2 years, 7 months ago)

How did you inoculate your grain? Spore syringe, agar, LC? Do you have any pictures of the spawn jars?


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OfflineBcuzz
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Re: stacking Monotub is causing problems [Re: MysticMycologist]
    #27781122 - 05/17/22 05:01 AM (2 years, 7 months ago)

Thank you for the questions.
At first im doing a new batch of mycelium dishes because as you can see im not happy with the look

Second, i used grain 2 grain and my mother jar was inoculated with LI Tek.

As i saied mostly the problem was why is one monotub almost done and the other one didnt even start

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OfflineDERRAYLD
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Re: stacking Monotub is causing problems [Re: Bcuzz]
    #27781135 - 05/17/22 05:14 AM (2 years, 7 months ago)

This is not related to your monotub stacking, we all stack monotubs.

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OfflineBcuzz
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Re: stacking Monotub is causing problems [Re: DERRAYLD]
    #27781433 - 05/17/22 10:41 AM (2 years, 7 months ago)

im sure youre 100% right, usually im not stacking my monotubs but it seems if im doing it im changing something and i dont know what exactly

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OfflinePBJ710
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Re: stacking Monotub is causing problems [Re: Bcuzz]
    #27781482 - 05/17/22 11:34 AM (2 years, 7 months ago)

I think it's your culture as well.  Do you have pics of the plates used to inoculate the LI?  Is this a proven fruiting culture or just a random set of genetics off a MS plate?

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OfflinePTFer
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Re: stacking Monotub is causing problems [Re: PBJ710]
    #27781509 - 05/17/22 12:03 PM (2 years, 7 months ago)

I always shuffle everything. Tubs, showboxes, grain jars, agar, even my feet. I also like to revolve everything. Thinking is to even out air, light, heat, etc. But just the same, in all likelihood you have some contam issues.


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OfflineBcuzz
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Re: stacking Monotub is causing problems [Re: PTFer]
    #27781665 - 05/17/22 02:18 PM (2 years, 7 months ago)

yea it might be the culture, this is from the F2 genetic wich mean its from the first clone i got:



i destroyed now these Batch because as you can see its not healthy, its not the first batch so its working but im not really happy with this clone.

this is also the batch from the jar:


so it works but well like i saied its still strange, this is the first time im stacking my monotub and the first time this happened.

Edited by Bcuzz (05/17/22 02:24 PM)

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InvisibleBaba Yaga
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Re: stacking Monotub is causing problems [Re: Bcuzz] * 1
    #27781723 - 05/17/22 02:58 PM (2 years, 7 months ago)

It's called coincidence. Jumping to conclusions after some random observation is the worst enemy in this hobby.

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Offlinesmalltalk_canceled
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Re: stacking Monotub is causing problems [Re: Baba Yaga] * 1
    #27781729 - 05/17/22 03:03 PM (2 years, 7 months ago)

Dont say that, once I saw a. Wait.:mushroom2:


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OfflineMushieMafia
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Re: stacking Monotub is causing problems [Re: smalltalk_canceled]
    #27781865 - 05/17/22 04:47 PM (2 years, 7 months ago)

i reckon its a spwan issue IMO


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OfflineBcuzz
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Re: stacking Monotub is causing problems [Re: MushieMafia]
    #27782820 - 05/18/22 10:22 AM (2 years, 7 months ago)

well well well it looks like the top box is really contaminated, could it be because there was a shitload of water on the top ?

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OfflineHindsight
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Re: stacking Monotub is causing problems [Re: Bcuzz] * 1
    #27782832 - 05/18/22 10:43 AM (2 years, 7 months ago)

No. High humidity isn't going to cause contamination. It was probably dirty grain spawn but also your room temp is 77F which is on the top end, and then when you add the heat of the lower boxes working their way up into the top box, your top box sub temp is probably in the low to mid 80's which is hot and gives a boost to bacteria.

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InvisibleMysticMycologist
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Re: stacking Monotub is causing problems [Re: Hindsight]
    #27782847 - 05/18/22 10:55 AM (2 years, 7 months ago)

:whathesaid:

Even slightly bacterial spawn could get nasty at those elevated temps, and really weaken the mycelium.


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OfflineBcuzz
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Re: stacking Monotub is causing problems [Re: MysticMycologist]
    #27783047 - 05/18/22 02:04 PM (2 years, 7 months ago)

this makes a lot of sense to me guys, im really great to got you all

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OfflineBcuzz
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Re: stacking Monotub is causing problems [Re: Bcuzz]
    #27786545 - 05/21/22 06:32 AM (2 years, 7 months ago)

Hey fellas,
a little Update: yea the "clean" one got a mold problem like some of you have forseen the consequences of the heat.

another question: i have some petri dishes but im showing just one, i have a "fizzy" mycelim grow and also it was a MS syringe, as you can see around the mycelium the white stuff is this also mold ? its in some Petri Dishes, this white stuff is growing where the water touched my agar


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InvisibleMysticMycologist
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Re: stacking Monotub is causing problems [Re: Bcuzz]
    #27786640 - 05/21/22 08:49 AM (2 years, 7 months ago)

I just want to chime in and clarify that your temp range wouldn’t be a problem if the spawn was anexic.  It is really looking like bacterial spawn is to blame. The elevated temps just helped the bacteria thrive.


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OfflineBcuzz
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Re: stacking Monotub is causing problems [Re: MysticMycologist]
    #27787006 - 05/21/22 02:35 PM (2 years, 7 months ago)

yea, youre right i thought maybe it will work out but this needs to be thrown away

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OfflinePBJ710
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Re: stacking Monotub is causing problems [Re: Bcuzz]
    #27787307 - 05/21/22 07:02 PM (2 years, 7 months ago)

Quote:

Bcuzz said:
Hey fellas,
a little Update: yea the "clean" one got a mold problem like some of you have forseen the consequences of the heat.

another question: i have some petri dishes but im showing just one, i have a "fizzy" mycelim grow and also it was a MS syringe, as you can see around the mycelium the white stuff is this also mold ? its in some Petri Dishes, this white stuff is growing where the water touched my agar






That cloudyness is bacteria.  Make sure that none of your transfers come from areas where this is present.  You can streak spores to a petri and it will use alot less liquid and therefore makes it more difficult for bacteria to grow while at the same time spreading out the desired mycelium colonies so you can easily pick and choose through the cultures.

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OfflineBcuzz
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Re: stacking Monotub is causing problems [Re: PBJ710]
    #27787937 - 05/22/22 09:35 AM (2 years, 7 months ago)

Thank you for the feedback PBJ710, sadly it means this is in the syringe because the clodyness is spawned where the water touched the agar "sad".

i hope i can extract some mycelium to get a clean petri dish

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