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Eclipse3130
Servant of the Fungi



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2022 Spring Psilocybin Cup 4
#27780114 - 05/16/22 11:16 AM (1 year, 8 months ago) |
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-------------------- "In The Material World One seeks retirement and grows Old In The Magical World One seeks Enlightenment and grows Wiser In The Miraculous World One seeks nothing and grows Lighter As we all tread the Homeward Path we will explore many Realms And one day... we will all Realize that all experiences are Simply Different ways in which The All-That Is Perceives Itself"
Edited by Eclipse3130 (06/01/22 11:46 PM)
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Bobbins
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Re: 2022 Spring Psilocybin Cup [Re: Eclipse3130] 1
#27781028 - 05/17/22 02:05 AM (1 year, 8 months ago) |
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This is interesting though these daft categories are just stupid and making the whole thing feel increasingly cheap and tacky.
Thanks for posting
-------------------- DeALeRsHrOoMs
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Eclipse3130
Servant of the Fungi



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Re: 2022 Spring Psilocybin Cup [Re: Bobbins]
#27781355 - 05/17/22 09:07 AM (1 year, 8 months ago) |
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Yeah one could say that, I personally don't mind it because it creates more winners for the community and the overall potency profiles are still displayed in order of category. I find it useful for personal reference and questions being answered than a well put out competition, that's the goal of it anyway. For instance aborts were proven to be substantially more potent than their non aborted counterparts here.
What's interesting is the levels of Psilocin you would normally see are quite low comparatively. For example the top 3 winners had 0.1%, 0.043% and 0.077% psilocin levels respectively.
All generally around 1.5% psilocybin, comparatively to the 2021 spring cup you would likely see 5-10x the amount of psilocin on average for these potencies(around 0.5% psilocin)
I find that interesting, generally the higher psilocybin you see you will also see psilocin tag along, it's just not the case here. Which leaves the question open are psilocin levels produced in their own quantities within the mushroom separately to psilocybin though they are produced in tandem, if we view the Panaleous Aguadilla that was submitted it had 7x higher Psilocin than its Psilocybin counterpart. This seems glaringly striking that Psilocin CAN be produced in its own quantities separately.
It seems quite obvious in the case of the Pan submitted that it is producing its own psilocin levels separately of psilocybin, but there's more questions at play still, maybe it could be lacking an enzyme(PsiK) that is responsible for polymerizing psilocin into psilocybin while it grows, because it also has the lowest reported Psilocybin level(0.1%) or, maybe genetically it is producing copious psilocin independently of enzymes.
Also, I'm sure different levels of tissue damage and drying/storage techniques could ultimately end up resulting in higher Psilocin levels as it also forms as psilocybin breaks down. In some cases I'm sure it's hard to determine the difference.
-------------------- "In The Material World One seeks retirement and grows Old In The Magical World One seeks Enlightenment and grows Wiser In The Miraculous World One seeks nothing and grows Lighter As we all tread the Homeward Path we will explore many Realms And one day... we will all Realize that all experiences are Simply Different ways in which The All-That Is Perceives Itself"
Edited by Eclipse3130 (05/17/22 09:46 AM)
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Icyurmt
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Re: 2022 Spring Psilocybin Cup [Re: Eclipse3130]
#27781516 - 05/17/22 12:09 PM (1 year, 8 months ago) |
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Really interesting to see the drastic variations between some of the weakest samples and the most potent. 🤯
Quote:
Also, I'm sure different levels of tissue damage and drying/storage techniques could ultimately end up resulting in higher Psilocin levels as it also forms as psilocybin breaks down.
I think this is most likely the case. I am very skeptical that psilocin can be produced independently of psilocybin in cubensis. As far as I'm aware, for cubes at least, the biosynthetic pathway has been established and any psilocin produced is a result of psilocybin dephosphorylation.
"psilocybin biosynthesis begins when tryptophan is decarboxylated by PsiD to form tryptamine. Tryptamine is then hydroxylated on the 4-position of the indole backbone by the cytochrome P-450 monooxygenase, PsiH, to form 4-hydroxytryptamine. The hydroxyl group of 4-hydroxytryptamine is next phosphorylated by PsiK to form norbaeocystin. Iterative methylation of the amine by PsiM then forms baeocystin, and then psilocybin. Psilocin is derived from the spontaneous dephosphorylation of psilocybin and can be converted back to psilocybin by PsiK."
 Diversity, biology, and history of psilocybin-containing fungi: Suggestions for research and technological development
Quote:
Which leaves the question open are psilocin levels produced in their own quantities within the mushroom separately to psilocybin though they are produced in tandem, if we view the Panaleous Aguadilla that was submitted it had 7x higher Psilocin than its Psilocybin counterpart. This seems glaringly striking that Psilocin CAN be produced in its own quantities separately.
I believe it's quite possible that other species like that panaeolus, could be producing psilocin independent of psilocybin, as they may have different biosynthesis routes/enzymes involved compared with cubes. To me that would make sense and could also explain why pan cinctulus for example don't bruise but are still relatively potent. This paper is pre-publish but it shows that some sp have different gene cluster arrangements for psilocybin production and therefore potentially different enzymes. It even suggests that different psilocybe sp could be producing novel psilocybin like compounds because of it. Convergent evolution of psilocybin biosynthesis by psychedelic mushrooms
"Additionally, in Psilocybe cyanescens there is a second monooxygenase (which we name here PsiH2) that is slightly different from the one found in all five species, as well as a third MFS-type transporter, all of which are expressed at the RNA level (Fig 1a). These findings hint at the exciting possibility that mushrooms from the Psilocybe genus, and particularly P. cyanescens, could be producing novel psilocybin-like molecules."
-------------------- 👁️ 🌊 why you are empty. Hunt for the habitat not the mushroom.
Edited by Icyurmt (05/17/22 12:18 PM)
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7Suns
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Re: 2022 Spring Psilocybin Cup [Re: Icyurmt]
#27792989 - 05/25/22 09:17 PM (1 year, 7 months ago) |
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How does one enter in this and how is this “legal” I thought it was only decriminalized and would you have to be in a decriminalized city to participate? And what are the requirements, could it be any psilocin/Psilocybe strain you grew or can it be other species like pan cyan, or gymnopilus etc etc
-------------------- Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage 7suns grain sac hack 7suns straw log hack 7suns official OTC antibiotic agar recipe 7suns DIY filter bags 7suns cheap AF flowhood 7suns cold weather fruit chamber
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Eclipse3130
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Re: 2022 Spring Psilocybin Cup [Re: 7Suns] 1
#27793211 - 05/26/22 03:36 AM (1 year, 7 months ago) |
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The lab is ran out of Oakland which is decriminalized. A group of people I think mostly Instagram based run it, the main guy being Oakland Hyphae. They also throw frequent psychedelic conferences at various locations which like minds meet to converge.. speakers, booths, chemists, cultivators, you name it gather to elevate as a community. I believe his purpose is to mostly be a force against big money trying to come into the space and take over like they did with cannabis, and to keep the mistakes of the cannabis industry ever happening within the psychedelic space as it grows, to keep its roots and culture protected and remembered, and to keep it decriminalized/medical use instead of legalized for as long as possible to keep corporate greed out.
Anyone can enter for a fee generally, sometimes included is tickets for the events as well. It's mostly Instagram based if you want to follow Oakland Hyphae you can keep up with the upcoming cups and events, and you should be able to submit through instruction at the time, any species is welcome, a Panaleous was submitted to this cup and others have in the past as well. You ship your dried sample to them in Oakland and they will run it through HPLC and compile the data for the cup
-------------------- "In The Material World One seeks retirement and grows Old In The Magical World One seeks Enlightenment and grows Wiser In The Miraculous World One seeks nothing and grows Lighter As we all tread the Homeward Path we will explore many Realms And one day... we will all Realize that all experiences are Simply Different ways in which The All-That Is Perceives Itself"
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7Suns
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Re: 2022 Spring Psilocybin Cup [Re: Eclipse3130]
#27793285 - 05/26/22 06:09 AM (1 year, 7 months ago) |
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Very informative thank you, I’m going to have to look into this, I’ve always wanted to test to see if the substrate makes a difference so if they allow me 2 submissions I might finally get answers
-------------------- Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage 7suns grain sac hack 7suns straw log hack 7suns official OTC antibiotic agar recipe 7suns DIY filter bags 7suns cheap AF flowhood 7suns cold weather fruit chamber
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Rotnpins
🤮 Rotten-Pins 🍄



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Re: 2022 Spring Psilocybin Cup [Re: Eclipse3130]
#27796186 - 05/28/22 08:27 AM (1 year, 7 months ago) |
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Alan Rockefeller
Mycologist

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Re: 2022 Spring Psilocybin Cup [Re: Eclipse3130]
#27800380 - 05/31/22 01:39 PM (1 year, 7 months ago) |
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Quote:
Eclipse3130 said: What's interesting is the levels of Psilocin you would normally see are quite low comparatively. For example the top 3 winners had 0.1%, 0.043% and 0.077% psilocin levels respectively.
All generally around 1.5% psilocybin, comparatively to the 2021 spring cup you would likely see 5-10x the amount of psilocin on average for these potencies(around 0.5% psilocin)
That is likely due to improved testing methodology. If the solvents aren't completely dry, psilocybin converts to psilocin.
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Eclipse3130
Servant of the Fungi



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That's what I would guess, so would it be reasonable to add the psilocin percentages of the 2021 spring cup to the psilocybin for a more precise psilocybin percentage?
It's also interesting that specifically the 2021 spring cup had a large range of very potent mushrooms comparatively to the following 3 cups. Do you believe these mushrooms were coming out higher than say they would if they were submitted to this cup, or by chance that cup just had tons of potent mushrooms submitted that we haven't seen since really.
112 samples this cup and only 3 had 1.5% psilocybin, whereas the 2021 cup had quite a few more potent mushrooms especially if taking into account converting the psilocin back into psilocybin.
Something seems odd, specifically that cup had an abnormal amount of potent mushrooms submitted and we just haven't seen that since for some reason
-------------------- "In The Material World One seeks retirement and grows Old In The Magical World One seeks Enlightenment and grows Wiser In The Miraculous World One seeks nothing and grows Lighter As we all tread the Homeward Path we will explore many Realms And one day... we will all Realize that all experiences are Simply Different ways in which The All-That Is Perceives Itself"
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Alan Rockefeller
Mycologist

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Re: 2022 Spring Psilocybin Cup [Re: Eclipse3130] 1
#27801541 - 06/01/22 02:05 PM (1 year, 7 months ago) |
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Quote:
Eclipse3130 said: That's what I would guess, so would it be reasonable to add the psilocin percentages of the 2021 spring cup to the psilocybin for a more precise psilocybin percentage?
Probably.
Quote:
It's also interesting that specifically the 2021 spring cup had a large range of very potent mushrooms comparatively to the following 3 cups. Do you believe these mushrooms were coming out higher than say they would if they were submitted to this cup, or by chance that cup just had tons of potent mushrooms submitted that we haven't seen since really.
My guess is that the testing methodology has gotten more accurate over time.
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Eclipse3130
Servant of the Fungi



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That's what I would assume. And that's a good thing! I just want to see some competition in the next cup I submitted an ape that wasn't even all that strong and it took 1st which means people aren't submitting strong samples for some reason, or rather not many people are growing potent variety
-------------------- "In The Material World One seeks retirement and grows Old In The Magical World One seeks Enlightenment and grows Wiser In The Miraculous World One seeks nothing and grows Lighter As we all tread the Homeward Path we will explore many Realms And one day... we will all Realize that all experiences are Simply Different ways in which The All-That Is Perceives Itself"
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QM33
(NOT A PUPPET!) ❤❤❤❤❤



Registered: 04/09/20
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Re: 2022 Spring Psilocybin Cup [Re: Eclipse3130]
#27801761 - 06/01/22 05:42 PM (1 year, 7 months ago) |
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Wait you got first this year?
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Eclipse3130
Servant of the Fungi



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Re: 2022 Spring Psilocybin Cup [Re: QM33] 3
#27801810 - 06/01/22 06:29 PM (1 year, 7 months ago) |
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Yeah that is my ape bc sample I submitted
-------------------- "In The Material World One seeks retirement and grows Old In The Magical World One seeks Enlightenment and grows Wiser In The Miraculous World One seeks nothing and grows Lighter As we all tread the Homeward Path we will explore many Realms And one day... we will all Realize that all experiences are Simply Different ways in which The All-That Is Perceives Itself"
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Rotnpins
🤮 Rotten-Pins 🍄



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Re: 2022 Spring Psilocybin Cup [Re: Eclipse3130]
#27802117 - 06/01/22 11:05 PM (1 year, 7 months ago) |
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Quote:
Eclipse3130 said: Yeah that is my ape bc sample I submitted
Congratulations! That's awesome!
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QM33
(NOT A PUPPET!) ❤❤❤❤❤



Registered: 04/09/20
Posts: 4,739
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Re: 2022 Spring Psilocybin Cup [Re: Eclipse3130]
#27802276 - 06/02/22 05:28 AM (1 year, 7 months ago) |
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Quote:
Eclipse3130 said: Yeah that is my ape bc sample I submitted
That's hilarious! I'm over hear searching IG for whoever BC is lol. Is the BC for blue cap or..?
Is it Sporeworks ape genetics and did you isolate them at all?
That's awesome bro. Goodjob! Putting the PNW on the map! I hope your from oregonhaha
-------------------- OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum... There are known knowns, there are known unknowns, there are also unknown unknowns. With great privilege comes great responsibility.
  Quantom Qups PROOF AND Soft Drops Turn your Swab to a Syringe and Syringe to Multiple Syringes! No Pours (QuantomStyal)Magic Fruit Leather DMT for IandI
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7Suns
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Re: 2022 Spring Psilocybin Cup [Re: QM33]
#27802315 - 06/02/22 06:27 AM (1 year, 7 months ago) |
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Should have led with that lmao I mean modesty is cool but I believe in giving credit when it’s due so congrats
Ps: I’m coming for your spot 2023
-------------------- Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage 7suns grain sac hack 7suns straw log hack 7suns official OTC antibiotic agar recipe 7suns DIY filter bags 7suns cheap AF flowhood 7suns cold weather fruit chamber
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QM33
(NOT A PUPPET!) ❤❤❤❤❤



Registered: 04/09/20
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Re: 2022 Spring Psilocybin Cup [Re: 7Suns] 3
#27802328 - 06/02/22 06:46 AM (1 year, 7 months ago) |
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Personally I think it's all hype. Especially if you look at how the cannabis cup was judged.
And idk I've heard sketchy stuff about Oakland hyphae even. And I know they have worked closely with Magic Myco who is a fucking fake.
Magic Myco is a perfect example of people with money who buy everything they need to and claim to be the best because of it. Dudes overcharge for garbage, spread misinformation and seem to be effecting the scene negatively because of their actions imo. And Oakland hyphae I'm sure is aware of this and similar situations in the bay area where this hype movement if originating.
Don't forget it's sacred medicine first and foremost fam. 90+ bucks for a swab, even 25 for a culture is insane
Looks what seeds go for. Remember how long it takes to grow a seed.
Remember how cheap it is and quick to make cultures.
Think for yourself. These big names are bought. And in time will be swept under the rug by those who are worthy.
-------------------- OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum... There are known knowns, there are known unknowns, there are also unknown unknowns. With great privilege comes great responsibility.
  Quantom Qups PROOF AND Soft Drops Turn your Swab to a Syringe and Syringe to Multiple Syringes! No Pours (QuantomStyal)Magic Fruit Leather DMT for IandI
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Eclipse3130
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Re: 2022 Spring Psilocybin Cup [Re: QM33] 4
#27802427 - 06/02/22 08:46 AM (1 year, 7 months ago) |
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The cup is great for getting questions answered, as well as some friendly competition. I personally submitted this ape to gauge vs the accuracy of the at home Psilo Q psilocybin test kits, which I can say they are damn near accurate which is awesome because they're only $20 each. I now feel comfortable and confident the numbers they spit back to me are 90%+ accurate vs HPLC
And yeah the genetic isn't all that stable so you have to clone and run spore frequently. Gotta stay real close to masters and back to spore or you'll run into aborts and reverts, BC stands for blue cap pheno, client names are on the left
I don't even feel like I won because I could of submitted a stronger mushroom I was honestly expecting the ape to place in the top 10. I just wanted fresh results comparing to the psilo Q test and this ape happened to be the most recent one I had tested with the last test kit I had on hand.
I can't say much about magic myco besides they do make a lot of genetics and I picked up some spores from them from their booth at the event for $10 each. $25 for an agar plate is a fairly normal price as well, especially if it's a great genetic. Doma the head of magic myco seems fairly educated at the least compared to some people
But yeah most of the scene is people in way too deep for their own good, trying to be big and run the mushroom world and cash out when they don't even have the skills or experience for themselves. I'm over here winning the cups and don't even have a desire to try and become big within the scene like some people do, I just work in silence and grow the best medicine I can. But at the end of the day there is fake people in any scene, overall what's happening is great for the mushroom itself and understanding it better.
We can say for certain now that Psilocin is a degradation byproduct of Psilocybin and they're not produced in tandem together. Any Psilocin seen in literature of dried fruit is from poor methodology or poor fruit handling and preserving process. Psilocin does not appear to be produced independently and lines up with the research that points to it being catalyzed back into psilocybin while the mushroom is growing by the kinase PsiK, any resulting psilocin seen is from the damage of picking, drying and preserving. The biosynthetic pathway to Psilocybin production in cubensis is ultimately a string of alkaloids that the end up culminating into Psilocybin.
The process looks like Tryptophan>Tryptamine>4HydroxyTryptamine>Norbaeocystin>Baeocystin>Psilocybin and ultimately Psilocin being a decarboxylated form of Psilocybin. Kinase PsiK has shown to catalyze Psilocin back into Psilocybin while the mushroom is growing. Enzymatic process helps convert and ultimately form psilocybin in the end. It makes sense to see small traces of these alkaloids in dry fruit.
Blueing is actually the result of enzymes breaking down psilocybin into oilgomer byproduct before ultimately turning into psilocin. It wasn't too long ago I thought blueing was a sign of psilocin potency, or potency in general. Sometimes it is true, but not always. It more so has to do with the structure/density/pigment of the mushroom itself and its overall enzyme content
-------------------- "In The Material World One seeks retirement and grows Old In The Magical World One seeks Enlightenment and grows Wiser In The Miraculous World One seeks nothing and grows Lighter As we all tread the Homeward Path we will explore many Realms And one day... we will all Realize that all experiences are Simply Different ways in which The All-That Is Perceives Itself"
Edited by Eclipse3130 (06/02/22 09:18 AM)
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QM33
(NOT A PUPPET!) ❤❤❤❤❤



Registered: 04/09/20
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Re: 2022 Spring Psilocybin Cup [Re: Eclipse3130]
#27802457 - 06/02/22 09:12 AM (1 year, 7 months ago) |
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Omfg. Ive looked at that same page several times the last month or so and everytime I saw the clients name I saw "mycelialnetwork".
MYCELICNETWORK!

Accept my friend request
-------------------- OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum... There are known knowns, there are known unknowns, there are also unknown unknowns. With great privilege comes great responsibility.
  Quantom Qups PROOF AND Soft Drops Turn your Swab to a Syringe and Syringe to Multiple Syringes! No Pours (QuantomStyal)Magic Fruit Leather DMT for IandI
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Eclipse3130
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Re: 2022 Spring Psilocybin Cup [Re: QM33]
#27802476 - 06/02/22 09:33 AM (1 year, 7 months ago) |
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Who dat?!
-------------------- "In The Material World One seeks retirement and grows Old In The Magical World One seeks Enlightenment and grows Wiser In The Miraculous World One seeks nothing and grows Lighter As we all tread the Homeward Path we will explore many Realms And one day... we will all Realize that all experiences are Simply Different ways in which The All-That Is Perceives Itself"
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QM33
(NOT A PUPPET!) ❤❤❤❤❤



Registered: 04/09/20
Posts: 4,739
Loc: Oregon
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Re: 2022 Spring Psilocybin Cup [Re: Eclipse3130]
#27802508 - 06/02/22 10:14 AM (1 year, 7 months ago) |
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-------------------- OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum... There are known knowns, there are known unknowns, there are also unknown unknowns. With great privilege comes great responsibility.
  Quantom Qups PROOF AND Soft Drops Turn your Swab to a Syringe and Syringe to Multiple Syringes! No Pours (QuantomStyal)Magic Fruit Leather DMT for IandI
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Healing Oakland
Mushroom Co-op

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Re: 2022 Spring Psilocybin Cup [Re: Eclipse3130]
#27809061 - 06/06/22 11:06 PM (1 year, 7 months ago) |
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Digging the great work Eclipse! 
-------------------- Healing Oakland The Magic Mushroom Co-operative Are you a cultivator in or near the Bay Area?
Healing Oakland is accepting new cultivators We are also accepting applications for co-op assistants and customer service reps.
OAKLAND DECRIMINALIZED
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Psilosophy23
Psychonaught


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Re: 2022 Spring Psilocybin Cup [Re: Eclipse3130]
#27826134 - 06/18/22 05:44 PM (1 year, 7 months ago) |
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Wow those are crazy results. When is the next psilocybin cup?
Edited by Psilosophy23 (06/18/22 06:19 PM)
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Eclipse3130
Servant of the Fungi



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Fall, I believe possibly in September of this year
-------------------- "In The Material World One seeks retirement and grows Old In The Magical World One seeks Enlightenment and grows Wiser In The Miraculous World One seeks nothing and grows Lighter As we all tread the Homeward Path we will explore many Realms And one day... we will all Realize that all experiences are Simply Different ways in which The All-That Is Perceives Itself"
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Psilosophy23
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Re: 2022 Spring Psilocybin Cup [Re: Eclipse3130]
#27826711 - 06/19/22 02:06 AM (1 year, 7 months ago) |
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Nice i will try to catch it
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Unknownfungi
Amateur mycologist



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praisetheking7
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Re: 2022 Spring Psilocybin Cup [Re: Eclipse3130]
#27871582 - 07/21/22 01:12 PM (1 year, 6 months ago) |
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Does anyone know how I could get my hands on one of the samples from the Psilocybin cup. MycelicNetwork had a sample off the charts. Ape Bc was the sample name. Thanks
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