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OfflineKevinspacey
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First time
    #27778272 - 05/15/22 12:59 PM (2 years, 7 months ago)

Hi guys, first time grower here and looking for some advice. I bought a kit and the spores. I inoculated the jars on April 29 and still no mycelium. I have another syringe, is it cool to put it into the same jars? Also I have them on a bookshelf in indirect light, should they be in total darkness? Any advice is greatly appreciated!

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OfflineJW123
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Re: First time [Re: Kevinspacey]
    #27778320 - 05/15/22 01:21 PM (2 years, 7 months ago)

Is it grain jars or PF tek? Either way don't waste your other syringe by innoculating something more than once.

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OfflineRotnpins
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Re: First time [Re: Kevinspacey]
    #27778361 - 05/15/22 01:42 PM (2 years, 7 months ago)

Are they PF Tek (brf) jars? If so, I'd be patient,  I've had jars take up to 3 weeks to start showing... What temperature are the jars being stored at? can you post a picture of your jar(s)? How many holes did you inoculate? 1/2/4? Were they toward the edges or the center of the jar?

I wouldn't use another syringe on the same jars.. if you're making your own jars, and not using pre-mades, I would  recommend making a second set of jars and getting them started.

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OfflineKevinspacey
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Re: First time [Re: JW123]
    #27778462 - 05/15/22 02:31 PM (2 years, 7 months ago)

They are grain jars

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OfflineKevinspacey
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Re: First time [Re: Rotnpins]
    #27778471 - 05/15/22 02:34 PM (2 years, 7 months ago)

Since it’s my first time I used premade grain jars. They had a rubber port where I stuck the needle and a small hole covered with micropore tape. To be safe I covered the rubber with micropore tape when I was done, I did 4 jars.

Edited by Kevinspacey (05/15/22 02:35 PM)

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Offlinehazyhorse
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Re: First time [Re: Kevinspacey]
    #27778540 - 05/15/22 03:07 PM (2 years, 7 months ago)

spore to grain is notorious for failing & sub par spawn. do PF tek (you don’t need a pressure cooker) or get a PC & get into agar. skipping crucial steps in this hobby will punish you


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OfflineRotnpins
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Re: First time [Re: hazyhorse]
    #27778550 - 05/15/22 03:11 PM (2 years, 7 months ago)

Quote:

hazyhorse said:
spore to grain is notorious for failing & sub par spawn. do PF tek (you don’t need a pressure cooker) or get a PC & get into agar. skipping crucial steps in this hobby will punish you




:whathesaid:
Updated PF Tek Guide

Edited by Rotnpins (05/15/22 03:26 PM)

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OfflineKevinspacey
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Re: First time [Re: Rotnpins]
    #27778817 - 05/15/22 05:11 PM (2 years, 7 months ago)

Thank you! I want to do the pf tek method and make my own jars but wanted to do this method first but I guess I’m going to jump in feet first! Is it difficult to make?

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InvisibleWyoMX
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Re: First time [Re: Kevinspacey]
    #27778830 - 05/15/22 05:19 PM (2 years, 7 months ago)

Nope pftek is simple as making brownies out of a box. Mix 3 ingredients and put into a jar. Steam those jars and then once there cool you inoculate. Just follow that updated pftek thread and you'll be golden. I would look around and decide how you want to fruit them as well. You've got the humidity chamber which to me seems to work really well, the classic sgfc which I'm not a fan of but others swear by and you can also shred the cakes and mix with coir in a shoebox. I've never done it but plenty of people have and had success while some don't like it. Just gotta figure out what you wanna try and give it a go.

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Offlinehazyhorse
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Re: First time [Re: Kevinspacey] * 1
    #27778831 - 05/15/22 05:19 PM (2 years, 7 months ago)

PF tek? not at all!! PF is super easy. you basically just steam jars of brown rice flower & then inject them with the syringe. if you don’t have a PC it’s definitely the way to go. if you do have a pressure cooker, you can totally try jumping into agar. it seems scary but it’s actually really simple once you try it out. if not, PF tek all the way!

edit: myco beat me to it, but yeah. it’s easy


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

Edited by hazyhorse (05/15/22 05:25 PM)

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OfflineKevinspacey
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Re: First time [Re: WyoMX]
    #27779043 - 05/15/22 07:04 PM (2 years, 7 months ago)

Thank you!

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OfflineKevinspacey
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Re: First time [Re: Rotnpins]
    #27780261 - 05/16/22 01:15 PM (2 years, 7 months ago)

The link is broke

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Offlinehazyhorse
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Re: First time [Re: Kevinspacey]
    #27780263 - 05/16/22 01:17 PM (2 years, 7 months ago)



--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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OnlineThe Tao
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Re: First time [Re: Kevinspacey]
    #27780292 - 05/16/22 01:39 PM (2 years, 7 months ago)

Quote:

Kevinspacey said:
Since it’s my first time I used premade grain jars. They had a rubber port where I stuck the needle and a small hole covered with micropore tape. To be safe I covered the rubber with micropore tape when I was done, I did 4 jars.



How much of the syringe did you inject into each jar? What was the variety of the mushroom?
Can you use this link to post some pictures?
https://www.shroomery.org/forums/showflat.php?Cat=&Board=255&Number=27127917&fpart=&PHPSESSID=

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OfflineKevinspacey
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Re: First time [Re: The Tao]
    #27780328 - 05/16/22 02:14 PM (2 years, 7 months ago)


The variety is golden teacher and I did about 2 mls per jar. It seems to me like the grains inside the jar should be wetter then they are. Going to try the pf tek method in the next few days, it just sucks I wasted those spores

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OfflineRotnpins
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Re: First time [Re: Kevinspacey]
    #27780331 - 05/16/22 02:16 PM (2 years, 7 months ago)

Quote:

Kevinspacey said:
The link is broke





Oops, sorry about that! Thanks hazy!

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OfflineRotnpins
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Re: First time [Re: Kevinspacey]
    #27780336 - 05/16/22 02:18 PM (2 years, 7 months ago)

Quote:

Kevinspacey said:

The variety is golden teacher and I did about 2 mls per jar. It seems to me like the grains inside the jar should be wetter then they are. Going to try the pf tek method in the next few days, it just sucks I wasted those spores





It happens... fortunately,  if you stick with this hobby, you'll have the ability to make your own spore prints and syringes after your first harvest.. soon you'll be able to provide yourself with a never ending supply of spores.

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OfflineKevinspacey
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Re: First time [Re: Rotnpins]
    #27780342 - 05/16/22 02:21 PM (2 years, 7 months ago)

That’s the goal!

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OnlineThe Tao
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Re: First time [Re: Kevinspacey]
    #27780372 - 05/16/22 02:43 PM (2 years, 7 months ago)

You still have an entire syringe plus a couple of mls from the one you used. Small loss and lesson learned.
Find some wide mouth 1/2 pint jars if you’re trying BRF tek. The jars that you have grain in have shoulders and you wouldn’t be able to remove the cakes.

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OfflineKevinspacey
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Re: First time [Re: Rotnpins]
    #27780690 - 05/16/22 07:00 PM (2 years, 7 months ago)

With the dry vermiculite layer, is there a certain amount you add or just enough to cover the surface of the cake? Also once I inoculate the jars I should cover the holes with micropore tape right? Sorry for all the questions, that post was a little vague on those points.

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OnlineThe Tao
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Re: First time [Re: Kevinspacey] * 2
    #27780788 - 05/16/22 08:18 PM (2 years, 7 months ago)

I bring the BRF to the bottom of the threads on the jar then add dry vermiculite to the top of the jar. I don’t use tape but other members do. I think the dry vermiculite is enough and tape restricts air.

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OfflineKevinspacey
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Re: First time [Re: The Tao]
    #27780798 - 05/16/22 08:30 PM (2 years, 7 months ago)

Thank you!

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OfflineTheTimelessDon
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Re: First time [Re: Kevinspacey]
    #27780836 - 05/16/22 09:16 PM (2 years, 7 months ago)

If you haven't seen them already, The RR videos walk you through the whole process very easy and he says some cool tips - i.e. wiping the inside of the jar after you put BRF in and before the layer of verm.

You said you had a second syringe?  Did the vendor send you an extra?  I've tried a few different vendors now, and if you are using the one I'm thinking of - did you get a buy one get one GT?

If so... Don't stop what you're doing at all!  Just allow me to plant a seed and say, both those syringes may be screwed up.  Complete guess and no proof of course.  That's just what I think, because it reminds me of the time when I was once sent 2 GT syringes and only ordered 1... 

So continue what you're about to do by making your own brf jars!  Know that you don't need a pressure cooker to work with agar, and agar into a BRF jar is sooo good.  It's also easier to make than the brf jars, you'll just need to add 2 more things to your cart. (Malt Extract and agar agar is what I use)  And that's all you need :smile:  You mix that up and cook in lil 4oz jars the same way you're about to do the pf tek and you don't even need to buy petri dishes or anything fancy.  This way - you don't have to worry about the syringes you have being a crap shoot forever. 

And if I can get a second opinion, could he shake these failed jars?  If there's no contamination, and probably just going to throw away, try shaking up a bunch to spread out ANY germination, if anything is happening inside there.  Lol, but only if someone else co-signs - because leaving it alone or throwing away are possibly two better options.

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OfflineRotnpins
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Re: First time [Re: TheTimelessDon]
    #27780852 - 05/16/22 09:32 PM (2 years, 7 months ago)

Like The Don said, stick with trusted vendors, for sure.. the first spore syringes I got were super clear, and I think they were also buy 1 get one free.. I really should have done a little more research instead of jumping on the first "good deal" that I saw. I still have one of them and was thinking about trying it out again to see if I can get anything to grow. :shrug:

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OfflineKevinspacey
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Re: First time [Re: TheTimelessDon]
    #27781258 - 05/17/22 07:55 AM (2 years, 7 months ago)

I actually ordered two, just in case. They came from a place called hidden forest that seemed pretty reputable. You can definitely tell there are spores in the syringes

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Re: First time [Re: Kevinspacey]
    #27781273 - 05/17/22 08:10 AM (2 years, 7 months ago)

Cool cool :thumbup: If you ever need to get a syringe in the future,  I definitely recommend checking out the trusted vendors... I don't think I can say which one, but one of them has really nice ones that are very dark and if you mention the shroomery they hook you up with a freebie.

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OfflineKevinspacey
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Re: First time [Re: Rotnpins]
    #27781333 - 05/17/22 08:53 AM (2 years, 7 months ago)

Is there a list of trusted vendors on this board?

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OfflineRotnpins
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Re: First time [Re: Kevinspacey]
    #27781337 - 05/17/22 08:56 AM (2 years, 7 months ago)

Yupp... right Here

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OfflineKevinspacey
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Re: First time [Re: Rotnpins]
    #27781369 - 05/17/22 09:16 AM (2 years, 7 months ago)

You’re the man, thank you!

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Re: First time [Re: Kevinspacey]
    #27781393 - 05/17/22 09:56 AM (2 years, 7 months ago)

Quote:

Kevinspacey said:
You’re the man, thank you!




No problem! Best of luck with the PF Tek. It's really easy, I'm sure you'll do great 👍

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OfflineKevinspacey
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Re: First time [Re: Rotnpins]
    #27785599 - 05/20/22 11:41 AM (2 years, 7 months ago)

Hey man, after you inoculate your jars, do you cover the holes or just let the dry vermiculite do the filter work? Starting my jars today

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Re: First time [Re: Kevinspacey]
    #27785642 - 05/20/22 12:18 PM (2 years, 7 months ago)

The dry verm will be enough.. I used to cover mine also, but I switched to 1 inoculation hole in the center, instead of 4 on the edges, and I was told that it wouldn't  provide enough FAE.

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OfflineTheTimelessDon
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Re: First time [Re: Rotnpins]
    #27785661 - 05/20/22 12:27 PM (2 years, 7 months ago)

+1 for the Verm just does it's thing.  I've tried leaving open holes and covering with the foil after.  Didn't notice any differences.  I still put foil back on after inoculating them, until the jars reach their final destination in the other room.  Then I take it off with tape holes still punctured, and never had any issues with contams getting in, let alone getting past verm.  It's a great technique for many reasons!

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Re: First time [Re: Rotnpins]
    #27785685 - 05/20/22 12:45 PM (2 years, 7 months ago)

Quote:

Rotnpins said:
The dry verm will be enough.. I used to cover mine also, but I switched to 1 inoculation hole in the center, instead of 4 on the edges, and I was told that it wouldn't  provide enough FAE.



One Center hole? How much do you inject and how long does the jar usually take to consolidate?

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Re: First time [Re: The Tao]
    #27785758 - 05/20/22 01:27 PM (2 years, 7 months ago)

I still do around 1cc.. I've only done like 40 jars this way so far, and it seems to take around the same time.. the main difference is that I don't let them consolidate for a week. My logic being that once you see mycelium on the sides, the jar should be ready since you started in the center..
I haven't really decided which way I like better.

I'm thinking about trying a batch of jars using a spore syringe, and a batch using a LC this weekend.. I haven't done LC yet, because I don't trust my cultures enough yet to make a LC for grain, but I figure PFtek is more resilient, so I'm going to give it a go.

Edited by Rotnpins (05/20/22 01:31 PM)

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OfflineKevinspacey
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Re: First time [Re: Rotnpins]
    #27795171 - 05/27/22 02:00 PM (2 years, 7 months ago)

Thanks again dude, I inoculated six days ago and can already see all my jars are starting to colonize, the vermiculite I got was pretty big chunks so I was worried but apparently the spores didn’t mind! Did four holes and just dry verm on top with no tape

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