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keeno
enthusiast



Registered: 06/01/11
Posts: 2,679
Loc: UK
Last seen: 1 month, 14 days
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Boozie]
#27772199 - 05/11/22 03:40 AM (1 year, 9 months ago) |
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Quote:
Boozie said:

Top left looks best for sure, but another clean transfer (or maybe even 2) might really give you added assurance. And like Weavie said, when you transfer, throw a lil wedge in a small tester jar while youโre at it. Not a lot to lose, might catch things early.
cheers Weavie and Boozie, I'm on it!

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MTZ
In between



Registered: 12/17/21
Posts: 702
Loc: Northern rockies
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Re: AGAR ENVY! (Anything and All things agar!) [Re: GhostHustler] 1
#27772241 - 05/11/22 04:56 AM (1 year, 9 months ago) |
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Now that is weird. Had some oysters Tam up about 6 plates before. Had company over. I was playing right beside one of my exit ducts. Makes me wonder how much spore I've inhaled. What do you guys give me for odds on bringing these forgotten plates back??


 I'd sure hate to lose a few of these. But no worries. It's shrooms. Damn. Fingers crossed. The problem is. They're potent AND pit out like a well trained whore. If you know what I mean. ๐๐๐๐ฅณ๐
-------------------- Rehears your death every morning and night. Only when you constantly live as though already a corpse will you find freedom in the martial way, and fulfill your duties without fault throughout your life. ~ The Book Of Samurai ........ I live in the void. I can go to the in between anytime I desire. I visit the spirt world.
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: AGAR ENVY! (Anything and All things agar!) [Re: MTZ] 1
#27772331 - 05/11/22 07:17 AM (1 year, 9 months ago) |
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Randomly googling around and found this gem. "Required reading" indeed.
Difco & BBL Culture Media Manual - Manual of Microbiological Culture Media 2nd Edition 2006
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Infinite Monkeys

Registered: 09/01/21
Posts: 276
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Re: AGAR ENVY! (Anything and All things agar!) [Re: sandman420]
#27772450 - 05/11/22 10:03 AM (1 year, 9 months ago) |
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The FDA have a "Bacteriological Analytical Manual" or "BAM" for short. It catalogues many agar and liquid culture (referred to as broth in the manual) recipes. I assume these recipes are used as a standard for labs all across the united states.
Each recipe is numbered. Potato Dextrose Agar, for example, is M127.
They have a few recipes that we will all be familiar (with a twist, it seems).
Quote:
M182 Malt Extract Agar (for culturing yeasts and molds)
Malt extract, powdered 20.0 g Glucose 20.0 g Peptone 1.0 g Agar 20.0 g Distilled water 1 liter
I can't resist sharing this recipe.
Quote:
M185 Sheep Blood Agar
Blood agar base (Oxoid No. 2) 95 ml Sterile sheep blood, defibrinated 5 ml
Where the fuck would one procure sterile sheeps blood lmao
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Rotnpins
๐คฎ Rotten-Pins ๐



Registered: 01/11/22
Posts: 4,738
Loc: in (front of) the hood
Last seen: 1 year, 2 months
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Quote:
Infinite Monkeys said: I can't resist sharing this recipe.
Quote:
M185 Sheep Blood Agar
Blood agar base (Oxoid No. 2) 95 ml Sterile sheep blood, defibrinated 5 ml
Where the fuck would one procure sterile sheeps blood lmao
I saw some sheep's blood agar for sale online once and I thought about asking if anyone had tried using them
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GhostHustler
Stranger



Registered: 04/26/22
Posts: 61
Last seen: 27 minutes, 43 seconds
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Quote:
Infinite Monkeys said: GhostHunter, that's cool as heck.
Do you recall when those dishes were made and the recipe you used?
Did you pour in front of a hood?
Prob 2ish months ago, a few still looked not-too-dry (that one included). Used FreshCapMushroom's agar video tek on youtube for the recipe (basic MEA I believe) and BOD's method for microwaving/PCing the bottle.
No filter (yet) and I'm super stocky so I opt to sanitize an entire 4x4 grow tent and do G2G/agar work in there rather than trying to navigate a SAB.
Somewhat open air but I generally avoid contams as long as I avoid having a smoke beforehand and make sure to change into fresh clothes.
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LotKid
Never.Trust.A.Prankster



Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
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Re: AGAR ENVY! (Anything and All things agar!) [Re: GhostHustler]
#27772521 - 05/11/22 11:00 AM (1 year, 9 months ago) |
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GhostHustler
Stranger



Registered: 04/26/22
Posts: 61
Last seen: 27 minutes, 43 seconds
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Re: AGAR ENVY! (Anything and All things agar!) [Re: GhostHustler]
#27772578 - 05/11/22 11:56 AM (1 year, 9 months ago) |
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One of my friends works in a microbiology lab, might fish it out of the trash tn and mail it over to her to see if its fungal or bacterial.
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Dendrocopos
Latin woodpecker



Registered: 11/02/21
Posts: 511
Loc: Chained in RR's cellar
Last seen: 1 year, 8 months
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
#27772598 - 05/11/22 12:13 PM (1 year, 9 months ago) |
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Does sheep blood agar give the mushies any superpowers i should l know about ?
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Lemgrub



Registered: 04/02/18
Posts: 624
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Dendrocopos] 2
#27772604 - 05/11/22 12:16 PM (1 year, 9 months ago) |
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Ghost, that's a fungus, bacteria don't show that growth pattern.
Sheep's blood is used for detecting pathogens as you can observe hemolysis.
-------------------- We getting that pirate bay, alien shishkebab cordycep money

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Seamonkey84
Stranger

Registered: 03/26/22
Posts: 506
Last seen: 1 month, 14 days
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Lemgrub]
#27772633 - 05/11/22 12:55 PM (1 year, 9 months ago) |
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A couple plates of cordyceps I forgot to stick in the fridge. Basically taken up the entire volume of the dish and lid, trying to bust out through the sides. Like I microwaved a marshmallow inside the plate
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GhostHustler
Stranger



Registered: 04/26/22
Posts: 61
Last seen: 27 minutes, 43 seconds
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Lemgrub]
#27772649 - 05/11/22 01:09 PM (1 year, 9 months ago) |
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I thought so too based off growth pattern but the lab friend said otherwise (ie theres been found bacteria showing rhizo growth), cant seem to find anything on traffic cone orange mycelium growing on google either.
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BagOfDicks
Procrastinator



Registered: 12/11/21
Posts: 419
Loc: PNW
Last seen: 2 months, 18 days
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Re: AGAR ENVY! (Anything and All things agar!) [Re: GhostHustler]
#27773247 - 05/11/22 09:39 PM (1 year, 9 months ago) |
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How much you guys paying for petris right now? I found 50 for $22.
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TheTimelessDon
The Don


Registered: 03/19/22
Posts: 194
Last seen: 1 year, 3 months
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Re: AGAR ENVY! (Anything and All things agar!) [Re: GhostHustler]
#27773270 - 05/11/22 09:54 PM (1 year, 9 months ago) |
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Quote:
GhostHustler said:

Cladosporium
I just came across an article online with a picture similar to this. Cladosporium pictures online show some craaazy looking growth. Congrats! Lol
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SingularFusion


Registered: 10/31/18
Posts: 1,225
Last seen: 2 hours, 3 minutes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: BagOfDicks]
#27773278 - 05/11/22 10:03 PM (1 year, 9 months ago) |
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bout 100 bucks for a box of 500 currently where I'm at, middle of the road quality
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ClusterB
Aspiring Myco-Dork


Registered: 09/16/21
Posts: 124
Loc: PNW
Last seen: 12 days, 7 hours
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Re: AGAR ENVY! (Anything and All things agar!) [Re: SingularFusion]
#27773445 - 05/12/22 01:55 AM (1 year, 9 months ago) |
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Quote:
Nef said: bout 100 bucks for a box of 500 currently where I'm at, middle of the road quality
I don't know if I've landed on a go to for dishes yet but I just ordered a box of 500 for about that price.
Are those polystyrene? Any big problems that are deal breakers?
-------------------- I used to do drugs. I still do, but I used to too. -Mitch Hedberg
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nektar61
Into SporePlay



Registered: 07/04/20
Posts: 3,242
Loc: Cube Satellite
Last seen: 4 days, 16 hours
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Re: AGAR ENVY! (Anything and All things agar!) [Re: ClusterB]
#27773533 - 05/12/22 05:15 AM (1 year, 9 months ago) |
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You want good inexpensive petris? These are good. It was about $110 with shipping for 500. https://www.southernlabware.com/petri-dish-polystyrene-90-x-15mm-sterile-500-cs.html
Work well, ship quick enough. Listing says 20 per bag, but arrived in bags of 10, not 20, so easier to not have leftovers when making up a batch. When getting down to the end, pour that last 10 thicker or thinner and it works. With 20 per bag that doesn't always work.
Also says 100 mm but measure as 90mm. And the URL has 90-x-15mm- in it.
So this place is bad at listing catalog info but sells good Petris. I like 90 cm better than 100 cm too. Use less agar, take up less room if you have a lot poured. I'm usually transferring or sending to grain before even a 90 mm is filled.
They have ventilation ribs (for her pleasure!) so wrap it well.
I know of one other person on here who also uses this vendor for Petris no problem.
Some I ordered from a better known place (and cost $70 more per 500) had about 10% of bags punctured. I didn't notice until had in storage for over three months, so too late to return. It may have happened from shipping, I don't know. But Southern is good.
-------------------- -NEW? Start here.
Edited by nektar61 (05/12/22 05:53 AM)
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BagOfDicks
Procrastinator



Registered: 12/11/21
Posts: 419
Loc: PNW
Last seen: 2 months, 18 days
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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] 1
#27773565 - 05/12/22 07:00 AM (1 year, 9 months ago) |
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Damn! Thanks guys! That would be half the price costed out.. just gotta cough up the dough might as well get a media bottle also!
It was my birthday, so I might as well..
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Enjoil



Registered: 10/29/20
Posts: 2,383
Last seen: 8 hours, 29 minutes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: BagOfDicks]
#27773894 - 05/12/22 11:49 AM (1 year, 9 months ago) |
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I still use condiment cupsโฆ Iโve had unwrapped ones do fine for long long long long time.
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Rotnpins
๐คฎ Rotten-Pins ๐



Registered: 01/11/22
Posts: 4,738
Loc: in (front of) the hood
Last seen: 1 year, 2 months
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Enjoil]
#27773898 - 05/12/22 11:53 AM (1 year, 9 months ago) |
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Quote:
Enjoil said: I still use condiment cupsโฆ Iโve had unwrapped ones do fine for long long long long time.
Do you have a link to the ones you use?
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