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OfflineNewbieAstro
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Help this dummy grow Golden Teacher
    #27773105 - 05/11/22 07:53 PM (2 years, 8 months ago)

Hi, so I am attempting my very first cultivation. I got a spore syringe with Golden Teacher and a growing kit that consists of:

1 Tall shoe box size tote
1 Myco bag (2Pounds) of pasteurized custom blend substrate. (Dehydrated elephant and horse manure, vermiculite, gypsum.)
1 Quart jar (1 Pound) of sterilized organic yellow corn with filter patch and injection port. (Jars are 3/4 full to leave room for gas exchange.)
2.7oz spray bottle of distilled water
1oz spray bottle of 99% Isopropyl alcohol
2 alcohol pads
2 pair of disposable gloves
1 black liner

And I also heard I need to sterilize the hell outta this room before I even start. Do I have everything that I need? What else do I need and how else can I prepare?

Again, the strain is Golden Teacher.

Edit: Also dealing with dry weather that ranges from 70-90 F degrees during that day and dips to 50 at night. CA.

Edited by NewbieAstro (05/11/22 07:54 PM)

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InvisibleAtomsplit
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Re: Help this dummy grow Golden Teacher [Re: NewbieAstro]
    #27773111 - 05/11/22 07:57 PM (2 years, 8 months ago)

You forgot your brf cakes or agar.

SAB?


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OfflineNewbieAstro
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Re: Help this dummy grow Golden Teacher [Re: Atomsplit]
    #27773117 - 05/11/22 08:00 PM (2 years, 8 months ago)

Yes sir, it is an SAB.

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InvisibleAtomsplit
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Re: Help this dummy grow Golden Teacher [Re: NewbieAstro]
    #27773128 - 05/11/22 08:04 PM (2 years, 8 months ago)

I personally would run the pftek or swipe some agar dishes.

Spore syringes are a crapshoot onto grain. Save yourself some time and run the pftek and/or agar.

Good luck!!!


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Offlinehazyhorse
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Re: Help this dummy grow Golden Teacher [Re: NewbieAstro] * 1
    #27773138 - 05/11/22 08:07 PM (2 years, 8 months ago)

you don’t need to sterilize your room. unless you work in a lab grade clean room, your efforts of sterilization are futile. you don’t even need gloves if your sterile work is on point. if you weren’t planning on using agar i highly recommend it. spore to grain has a notoriously bad failure rate, & it’s better to skip it if you don’t already have bags/jars going. it sounds like you have a jar, but in case it’s a bag… bags can be really tricky to work with in an SAB & i’d recommend jars over bags if you aren’t working in front of a hood. im not saying it can’t be done, but bags kinda ruin the still air part of the still air box if you’re not really careful & have a larger than average SAB. if you don’t want to do agar (which isn’t that hard, i promise it seems more intimidating than it really is), you should do PF tek & then spawn the cakes to a shoebox of coco coir or your manure mixture

also elephant dung? that’s probably the most exotic thing i’ve heard of to grow mushrooms lol. in the future coco coir is probably your best bet. doesn’t need sterilization/pasteurization & works like a charm.


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Offlinenormalperson
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Re: Help this dummy grow Golden Teacher [Re: NewbieAstro] * 1
    #27773139 - 05/11/22 08:07 PM (2 years, 8 months ago)

If you have enough money to"sterilize ... this room" you'd be better off having your private jet diliver fresh picked shrooms from Mexico. Just saying that is a waste of time. Get a still air box instead.

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OfflineNewbieAstro
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Re: Help this dummy grow Golden Teacher [Re: hazyhorse]
    #27773151 - 05/11/22 08:15 PM (2 years, 8 months ago)

Ok, so say I want to use Agar. Would I make my own mason jar of it and have to create an injection port like the 1 Quart jar (1 Pound) of sterilized organic yellow corn? But instead of corn, it'll be agar then?

Ah, found a guide. Never mind.

Edited by NewbieAstro (05/11/22 08:20 PM)

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InvisibleLotKid
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Re: Help this dummy grow Golden Teacher [Re: NewbieAstro] * 1
    #27773170 - 05/11/22 08:32 PM (2 years, 8 months ago)

Quote:

NewbieAstro said:
Hi, so I am attempting my very first cultivation. I got a spore syringe with Golden Teacher and a growing kit that consists of:

1 Tall shoe box size tote
1 Myco bag (2Pounds) of pasteurized custom blend substrate. (Dehydrated elephant and horse manure, vermiculite, gypsum.)
1 Quart jar (1 Pound) of sterilized organic yellow corn with filter patch and injection port. (Jars are 3/4 full to leave room for gas exchange.)
2.7oz spray bottle of distilled water
1oz spray bottle of 99% Isopropyl alcohol
2 alcohol pads
2 pair of disposable gloves
1 black liner

And I also heard I need to sterilize the hell outta this room before I even start. Do I have everything that I need? What else do I need and how else can I prepare?

Again, the strain is Golden Teacher.

Edit: Also dealing with dry weather that ranges from 70-90 F degrees during that day and dips to 50 at night. CA.



holy fuck.... that's a "kit" someone is prepping and selling?

gawd dayum i gotta get in on this


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:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
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OfflineNewbieAstro
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Re: Help this dummy grow Golden Teacher [Re: LotKid]
    #27773182 - 05/11/22 08:40 PM (2 years, 8 months ago)

Currently buying some agar and watching videos. This stuff really does start to feel like a lab experiment. Although I am trying to wrap my head around dissecting and isolating to a new agar dish to keep contaminations off and keep it monoculture.

I am a little concerned as to where to store the spore syringe at the mean time while I wait for my agar.

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Offlinehazyhorse
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Re: Help this dummy grow Golden Teacher [Re: NewbieAstro]
    #27773184 - 05/11/22 08:46 PM (2 years, 8 months ago)

saw you said you found a guide, but just in case this wasn’t the one you found pastywhyte’s agar tek is a really good place to start. no pours are super simple


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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OfflineNewbieAstro
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Re: Help this dummy grow Golden Teacher [Re: hazyhorse]
    #27773218 - 05/11/22 09:20 PM (2 years, 8 months ago)

I also got some supplies from shroomsupply. Their basic agar kit that consists of: Two packs of four pre-poured malt extract agar petri dishes (eight dishes in total)
Alcohol Burner Lamp with wick (optional upgrade to wickless lamp available)
Surgical Steel Scalpel with five blades, and 10 Inoculation Loop - Sterilized apparently via gamma irradiated.

And a Glovebag Portable Still-Air Glovebox for a semi-sterile environment.

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InvisibleAtomsplit
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Re: Help this dummy grow Golden Teacher [Re: hazyhorse]
    #27773226 - 05/11/22 09:25 PM (2 years, 8 months ago)

You might as well run the pftek and learn about agar at the same time.

Although worth the time. You will most likely spend some time dialing in your agar game.

You will use 8 plates really fast! I swipe 4-5 using a print. Then you still have some cleaning to do.

Make & pour your own.


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Offlinehazyhorse
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Re: Help this dummy grow Golden Teacher [Re: NewbieAstro] * 1
    #27773230 - 05/11/22 09:27 PM (2 years, 8 months ago)

glove boxes are worse than an SAB, you’ll wanna take the gloves off. it causes more air movement inside the box


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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OfflineNewbieAstro
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Re: Help this dummy grow Golden Teacher [Re: Atomsplit]
    #27773232 - 05/11/22 09:30 PM (2 years, 8 months ago)

Probably will have to if this goes poorly. I am a total newbie at this and never actually tried mushrooms, and I heard this can yield to an ounce if things go well? I don't really know what I'd do with an ounce to be honest. But yeah, I currently only have one syringe since this is my first attempt at all. I heard prints are easier to work with?

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OfflineNewbieAstro
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Re: Help this dummy grow Golden Teacher [Re: hazyhorse]
    #27773234 - 05/11/22 09:30 PM (2 years, 8 months ago)

Ah, I was going to use it to handle the material rather than store it inside the glovebox.

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Offlinehazyhorse
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Re: Help this dummy grow Golden Teacher [Re: NewbieAstro]
    #27773276 - 05/11/22 09:59 PM (2 years, 8 months ago)

i like prints better personally. you can easily grow pounds if you really want. a shoebox can easily yield an ounce +. trust me you def end up with more than you know what to do with lol. i give a lot of mine away

you do all your sterile work inside the SAB, you don’t need to store anything inside the glove box. you use the SAB to open agar plates/jars without the risk of contamination (if your sterile technique is good). you can store plates & jars out in the open as long as you don’t open them


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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InvisibleBiggDaddyCane
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Re: Help this dummy grow Golden Teacher [Re: NewbieAstro]
    #27773402 - 05/12/22 12:00 AM (2 years, 8 months ago)

I know its been said but I'll drop in to say Agar.

Find some small Air tight containers, they don't need to be specific. I've seen people do agar in rubbermaids. Be sure its clean and spray down with alcohol and air dry.

Search a cheap Agar recipe.

It's smarter money wise to trash a few grams of Agar then trashing multiple jars and bags of corn, rye, or wbs.


Build a flow hood or SAB.

I build my own flow hood. Out of a blower fan attach to a box with two cheap HEPA Filters on the end. Maybe cost me 100 bucks for everything tops. An my Agars come out fine.

Still Air Box or Custom Flow Hood... As long as the end result is little to no contamination roll with it. If you still get contam agars with SAB or your flow hood. Tweak your methods.

I've seen some shitty custom flow hoods that users upload and said still work. If it works, it works....... I personally don't like SAB's over Filtered Fresh Air.

Get agar an a sterile environment setup an follow tek an you will be fine.


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OfflineGarryJarcia
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Re: Help this dummy grow Golden Teacher [Re: Atomsplit]
    #27773599 - 05/12/22 07:43 AM (2 years, 8 months ago)

Quote:

Atomsplit said:
Make & pour your own.



:whathesaid:


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OfflineRotnpins
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Re: Help this dummy grow Golden Teacher [Re: NewbieAstro]
    #27773713 - 05/12/22 09:42 AM (2 years, 8 months ago)

Quote:

NewbieAstro said:
Probably will have to if this goes poorly. I am a total newbie at this and never actually tried mushrooms, and I heard this can yield to an ounce if things go well? I don't really know what I'd do with an ounce to be honest. But yeah, I currently only have one syringe since this is my first attempt at all. I heard prints are easier to work with?




Just so you have realistic expectations.. getting an oz of dry will take a while, I wouldn't expect very high yields at first... I just broke a dry ounce in 1 flush, but it took 4- 1/2 pint cakes shredded to bulk using a shoebox tek...

I think I read the same thing you did before I started. It said you can expect up to an ounce per cake.. so when I ended up with just a few grams per cake, I was really disappointed with my first grow.. just try and keep your expectations realistic, and stick with it.. if you put in the hard work, eventually it will pay off and you'll be able to produce consistent results.

:goodluck::cheers:

Updated PF Tek:
https://www.shroomery.org/forums/showflat.php/Number/24179086

Bod's comprehensive agar tek:
https://www.shroomery.org/forums/showflat.php/Number/21922023

Bod's cleaning and isolation agar walkthrough:
https://www.shroomery.org/forums/showflat.php/Number/25347656

D3's perfect transfer and agar tek
https://www.shroomery.org/forums/showflat.php?Cat=&Board=2&Number=26628234&fpart=&PHPSESSID=

Also, this is an excellent thread to skim through and to participate in discussions about agar... I'd recommend following this thread:
https://www.shroomery.org/forums/showflat.php/Number/25662166


Edited by Rotnpins (05/12/22 09:47 AM)

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