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Offlinehazyhorse
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27770598 - 05/10/22 01:13 AM (2 years, 8 months ago)

i think you’ll get fruits man. you can get away with less than stellar stuff & get fruits which is nice. the hard part is getting it to perform super well. all just about dialing things in


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OfflineA.k.aM
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Re: Spores to Mycelium with Grain in 2 days [Re: hazyhorse]
    #27770813 - 05/10/22 07:45 AM (2 years, 8 months ago)

Yeah you’ll almost definitely get a decent yield off those.


The focus on shroomery is mainly on doing things in a clean and reliable method, but if you just want some fucking mushrooms already then syringes are fine. Quick and (possibly) dirty methods are effective if youre not doing this as a serious hobby.


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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: A.k.a]
    #27771258 - 05/10/22 01:59 PM (2 years, 8 months ago)

Well I’ve got 12 jars of good looking agar cultures now so if my tubs don’t do well I think I’ll be okay I can just try again.

And thanks if I can just get a couple to clone with I’ll be happy.

I think my technique has been pretty good, besides the mistake of wet grain. My main mistake was doing spore to grain but I really do think the syringe was pretty clean because my spore to agar jars don’t seem to have any contamination yet but they’ve already germinated and started growing.

I’m pretty skeptical on those though and I have them put aside in case they do contaminate.

Anyway can’t wait for the next run I already got 12 more quarts of grain spawn PCd and ready to go as of this morning.

I really want to see if I can get a haploid from the ghost and pink Buffalo and try to breed the two, that would be pretty cool

But yeah once I get everything dialed in and fine tuned I think I want to run some very controlled experiments and document them all

Edited by UnbornCaius (05/10/22 02:38 PM)

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InvisibleBiggDaddyCane
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27771294 - 05/10/22 02:24 PM (2 years, 8 months ago)

Quote:

UnbornCaius said:








I love to see people experimenting with different casing layers, I may try this on one of my tubs.

Your methods an success are intriguing. Don't worry about some comments here. Most people have a " ONLY USE WHAT WORKS " attitude.

If you are using an method that hasn't be seen or hasn't been perfected keep documenting it.

Just me personally I would never ever go straight to grains, no matter how clean or reliable my spores are. Your Myc growth looks good. Keep this updated.


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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: BiggDaddyCane]
    #27771308 - 05/10/22 02:36 PM (2 years, 8 months ago)

Thanks Bigg, lesson learned about spore to grain, and I will continue to document everything I do. I am having a lot of ideas already about different experiments I could run, and I’ve been writing them down in my log journal.

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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27771372 - 05/10/22 03:50 PM (2 years, 8 months ago)

Spore to grain was the main method used for a long time.

When I started I probably ran 30 jars from syringe with no issues, then when I started agar lost like 1/3 :lol:


Gotta have the sterile technique down.


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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: A.k.a]
    #27771383 - 05/10/22 03:57 PM (2 years, 8 months ago)

It really shows how much of a miracle it is that these things thrive in the wild when competing with the endless waves of bacteria, molds, yeast, animals, etc…

Life is insanely vast and impressive

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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27772098 - 05/11/22 12:14 AM (2 years, 8 months ago)

So fingers still crossed that there’s no contamination.

I kind of wanted to give it a little more air since after I put the filters in I haven’t needed to mist, so I took the lids off and fanned them. While I did that I took a couple photos.

There is a photo here of the PB tub with tape on the holes, but I just put filters on it since it started a couple days later than the ghost tub

I checked for a smell but couldn’t smell much of anything at all besides a faint mushroom/substrate/rain smell


Edited by UnbornCaius (05/11/22 12:38 AM)

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Offlinehazyhorse
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27772486 - 05/11/22 10:31 AM (2 years, 8 months ago)

you don’t need to fan at all. it doesn’t help anything & your air should be exchanged passively & consistently through dialing in your tubs. both tubs look dry but that first tub looks super dry to me but it could be the pic. you want your surface to look like this at practically all times


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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: hazyhorse]
    #27772723 - 05/11/22 02:19 PM (2 years, 8 months ago)

Thanks hazy, yeah I never fanned it or even opened the lid until then so I was surprised it was that dry. I’ve been misting a couple times a day now and just letting it evaporate on its own

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Offlinehazyhorse
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27772901 - 05/11/22 04:53 PM (2 years, 8 months ago)

no problem! yeah, dialing in your tubs can be tricky at first. i find it’s good to check in on stuff while it’s colonizing until you get a feel for how your tubs perform in your specific climate. ideally you shouldn’t have to mist until the first flush starts to come in, but if you haven’t run too many tubs you might not know how much to open the holes. polyfill & giant holes covered in micropore tape can be tricky to dial in. i’ve had really good success with  pasty’s ez dialed monos & just some micropore tape over the lower/upper holes. if they seem to want more FAE as the flush comes in, i’ll take off the tape. lately i’ve been able to just let it ride with no interference on my part

just FYI if your subs get too dry, they stop holding moisture on the surface in beads, so if you’re misting a lot but don’t notice the tiny beads after the mist, that may be why.


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you're not the first to set foot here, just another
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i love glass petris & you can too!!
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new to mushroom cultivation?? read this!!
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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: hazyhorse]
    #27773014 - 05/11/22 06:52 PM (2 years, 8 months ago)

Thanks hazy I’ll check it out!

Since I’ve misted last night I checked on them today and it looks like there is still a consistent layer of mist beads sitting on top of the mycelium lawn.

So the advice is to just maintain that level of moisture on the surface and just let the FAE evaporate the moisture on its own?

And yeah my filters can definitely be improved and dialed in quite a lot more than they are. I used two different styles of filters for the tubs. Lots of learning to do

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Offlinehazyhorse
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27773040 - 05/11/22 07:14 PM (2 years, 8 months ago)

Quote:

UnbornCaius said:
Since I’ve misted last night I checked on them today and it looks like there is still a consistent layer of mist beads sitting on top of the mycelium lawn.

So the advice is to just maintain that level of moisture on the surface and just let the FAE evaporate the moisture on its own?




yup!! that’s pretty much exactly what you wanna do (:

it’s always a learning process & as long as you’re paying attention you’ll get better with every grow. eventually you get things figured out to the point where it’s mostly dialed in. everyone’s conditions are different so there’s almost always a bit of work to be done before you can really make stuff set & forget


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you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: hazyhorse]
    #27773131 - 05/11/22 08:05 PM (2 years, 8 months ago)

Cool thank you! I’m looking forward to getting to the point where I can start reliably experimenting and getting meaningful data

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Offlinehazyhorse
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27773140 - 05/11/22 08:08 PM (2 years, 8 months ago)

just have fun with it! you’ll end up with more mushrooms than you’ll ever need way faster than you realize lol


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you're not the first to set foot here, just another
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i love glass petris & you can too!!
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new to mushroom cultivation?? read this!!
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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: hazyhorse]
    #27773240 - 05/11/22 09:35 PM (2 years, 8 months ago)

Good haha exactly what I’m looking for

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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27775080 - 05/13/22 04:33 AM (2 years, 8 months ago)

Here’s an update

Just misted


Ghost tub on the left, pink Buffalo on the right



Also miraculously my 6 grain to agar jars are totally contamination free as far as I can tell

My 6 spore to agar jars also seem to be totally contamination free, the only thing I see is pretty healthy rhizomorphic mycelium

Also it must have been a fluke that the ghost colonized faster, because the pink buffalo agar jars totally reached 100% before the ghost ones and the spores also took off faster

Edited by UnbornCaius (05/13/22 04:33 AM)

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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27779821 - 05/16/22 04:57 AM (2 years, 7 months ago)

Update on ghost tub

First pins showing

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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27787744 - 05/22/22 03:39 AM (2 years, 7 months ago)

So the “ghost” tub doesn’t seem to be ghost whatsoever.

The pink Buffalo tub just started pinning today

And I think there are FAE issues because some are growing sideways.


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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27787745 - 05/22/22 03:41 AM (2 years, 7 months ago)

The picture isn’t from today but a couple days ago, and about half of them have been harvested. Not a lot of yield on the first flush I would say there is almost an ounce dried so far

Edited by UnbornCaius (05/22/22 07:06 AM)

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