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InvisibleBigwormS
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Melmak spores to agar problem
    #27750681 - 04/25/22 01:41 PM (2 years, 8 months ago)

I swabbed some Melmak TP spores to some agar about 10 days ago and see nothing but the streaks left on the agar from the swab scratching it. I scraped the swab with a scalpel over one plate as well as cut some of the swab off and left it on the agar in a few plates. Still nothing. Any ideas? Keeping temps around 21c/70f. I'm really hoping these germinate.

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Invisiblefungusul
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Re: Melmak spores to agar problem [Re: Bigworm] * 1
    #27751153 - 04/25/22 07:29 PM (2 years, 8 months ago)

What recipe have you used for agar?

:imo: It's preferred to use a softer 1.5-1.7% agar to favor spore germination. Harder 2% agar can take longer for spores to germinate.

You could also try to cut and insert swab tip into agar. It worked for me and other shroomers had good success using this technique.

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InvisibleMTZ
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Re: Melmak spores to agar problem [Re: Bigworm] * 1
    #27751170 - 04/25/22 07:36 PM (2 years, 8 months ago)

Are you buying those janky rippoff plates the vendor hacks are scamming people with these days? Could be your problem. My macs, Mac tp etc are generally real fast to germ.


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InvisibleBigwormS
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Re: Melmak spores to agar problem [Re: MTZ]
    #27751938 - 04/26/22 10:45 AM (2 years, 8 months ago)

Fungusul, I used PDY with 2% agar from my last pour. Do you think the food coloring may be doing something? I just started agar about 3 weeks ago so they aren't really old. I did slice the agar open and wedge a piece of the swab I cut off into it. Not a big piece but I can see the fibers sticking up out of it. I'll give it a few more days before I start really worrying.

MTZ no I'm not using some vendor hack plates. I just started agar and so far only had 1 contaminate after the parafilm split and the plate opened. I lost a clone I was hoping to grow out but I have a few more so I'm good. I think my clone is looking good. It's way cheaper to pour my own.


Edited by Bigworm (04/26/22 11:41 AM)

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Invisiblefungusul
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Re: Melmak spores to agar problem [Re: Bigworm] * 1
    #27752179 - 04/26/22 01:43 PM (2 years, 8 months ago)

Quote:

I used PDY with 2% agar from my last pour.




It should be okay to use though it's preferable to be 1.7%.

Quote:

Do you think the food coloring may be doing something?




Depends on food coloring used. There are some problems with red food coloring which contains something toxic for mycelium. I'm using Club House Green without any issues.

Quote:

I'll give it a few more days before I start really worrying.




If you aren't forced to reuse those agar dishes, you could wait longer.

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Offlinehazyhorse
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Re: Melmak spores to agar problem [Re: fungusul] * 1
    #27752235 - 04/26/22 02:35 PM (2 years, 8 months ago)

spores can sometimes just take awhile too, so on top of stuffer agar it could just be prolonged. 10 days is nothing. i’d start to be concerned after 3 weeks. sometimes fresh spores germ fast but older spores can take a bit sometimes


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InvisibleBigwormS
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Re: Melmak spores to agar problem [Re: hazyhorse]
    #27752526 - 04/26/22 06:08 PM (2 years, 8 months ago)

Wow. I had no idea it could be that long. Reading some posts I seen a few days so I thought 10 days I should have seen something. I have no problem waiting now that I know it can take a long time. I'll just forget about them for a little. It's just normal blue food coloring and no rush on plates. I probably won't be reusing then anyway. Thanks everyone!

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Re: Melmak spores to agar problem [Re: Bigworm] * 1
    #27752894 - 04/27/22 03:50 AM (2 years, 8 months ago)

It helps to hydrate swabs with 1 drop of water. Ill purposely pour 1 plate extra hot so I have condensation. Steak 3 plates then bust the swab an inch from the end and jab that sucker in the 4th plate.

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Offlinehazyhorse
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Re: Melmak spores to agar problem [Re: 0001001] * 1
    #27753416 - 04/27/22 01:58 PM (2 years, 8 months ago)

fresh spores can germ super fast, but older spores can be really slow. if you were to put a fresh print to agar, you’d probably be surprised, try a print that’s 2 years & you might be waiting a bit. but as long as there aren’t contams on the plate & it’s not super stiff they’ll probably germ after awhile


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InvisibleBigwormS
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Re: Melmak spores to agar problem [Re: hazyhorse]
    #27753554 - 04/27/22 03:48 PM (2 years, 8 months ago)

0001001, the last of my plates did have condensation which I did knock down to the agar and used that to hydrate the spores. I didn't want to use tap water and didn't have any steril water but I figured it would help. I didn't want to cut the swab yet, plus I see nothing but contaminated plates when people do that. Have you had luck leaving the swab end in there with no contaminates?

Hazyhorse I am still learning patience. I thought I was a patient person until I started this hobby. You have truly been helpful with all my stupid questions since I've joined. Thank you!

I will update if/when things happen. I got a tidalwave syringe on the way as a back up as well so I'll be putting that to agar once I get it. Hopefully that goes well. Thank you all for the help.

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Offlinehazyhorse
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Re: Melmak spores to agar problem [Re: Bigworm] * 1
    #27753578 - 04/27/22 04:14 PM (2 years, 8 months ago)

in my experience with swabs, i’ve never had contams come from putting the whole swab in. i’m sure it can happen, but i honestly just put iso on my hands & use bare fingers to put the swab on the agar & don’t have issues.

mycology is really great for teaching you what actual patience looks like for sure lol. i totally feel ya. i’m always happy to help & i know it can be a lot to take in while getting started, so don’t sweat it (: i’m still learning things myself & no one is every truly a master


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Invisible0001001
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Re: Melmak spores to agar problem [Re: Bigworm] * 1
    #27753581 - 04/27/22 04:15 PM (2 years, 8 months ago)

Ive had good luck jabbing the swab into the agar. Once you have mycelium transfer away the leading edge a couple times to clean it up.

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InvisibleBigwormS
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Re: Melmak spores to agar problem [Re: 0001001]
    #27753735 - 04/27/22 05:38 PM (2 years, 8 months ago)

I still have 1 swab left so if these 8 plates fail I will give it another shot and jab the last plate with the tip.

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Re: Melmak spores to agar problem [Re: Bigworm] * 1
    #27753747 - 04/27/22 05:43 PM (2 years, 8 months ago)

Do contams usually grow faster than myc? Maybe it’s better for slow germ so you can spot any contams before the cube spores germ?

I don’t know but it sounds right, anybody know?


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Offlinehazyhorse
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Re: Melmak spores to agar problem [Re: san pedro guy] * 1
    #27753767 - 04/27/22 05:56 PM (2 years, 8 months ago)

contams generally move pretty fast in my experience. if you swabbed a germ plate & see contams before myc it’s musically pretty fucked. i’d want my germ plates to germ faster than contams personally, but the whole point of agar is to clean up cultures so i’m sure as long as you have myc that doesn’t have an insane sporeload from contams along with it you’re probably good to just transfer


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you're not the first to set foot here, just another
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InvisibleBigwormS
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Re: Melmak spores to agar problem [Re: hazyhorse]
    #27766170 - 05/06/22 03:26 PM (2 years, 8 months ago)

Something is finally happening! It's on a spot I really pushed the swab into when inoculating the agar. I'm not sure but I think it looks ok so far. What's everyone else think. Hopefully it's not a contam growing instead of myc. This is the only spot out of all my plates that has anything goin on right now.

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Re: Melmak spores to agar problem [Re: Bigworm] * 1
    #27769384 - 05/09/22 03:36 AM (2 years, 8 months ago)

the pic is a little hard to see but it looks fine to me. did it grow out at all?


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you're not the first to set foot here, just another
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i love glass petris & you can too!!
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new to mushroom cultivation?? read this!!
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InvisibleBigwormS
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Re: Melmak spores to agar problem [Re: hazyhorse]
    #27769724 - 05/09/22 12:04 PM (2 years, 8 months ago)

It is getting a bit larger. I plan on transferring in the next day or two from MEA to PDA with blue tint to see how it looks.

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InvisibleBigwormS
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Re: Melmak spores to agar problem [Re: hazyhorse]
    #27771870 - 05/10/22 08:34 PM (2 years, 8 months ago)

So I think this looks ok. I have not transferred to a blue plate yet since I see a satellite growth around the 9 o' clock position. I put the plate over a blue plate for more contrast. I was thinking taking 3 samples from the 3 o'clock position and putting on 3 separate plates to see how it goes. It should I wait to see what the secondary growth is and transfer that as well?

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Invisiblesandman420
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Re: Melmak spores to agar problem [Re: Bigworm] * 1
    #27771948 - 05/10/22 09:41 PM (2 years, 8 months ago)

transfer now


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