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GermanShaman
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Registered: 01/05/18
Posts: 343
Last seen: 10 months, 28 days
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When is the best time to use a LC to inoculate Grains?
#27770101 - 05/09/22 04:59 PM (2 years, 8 months ago) |
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If I go from an good clean agar culture to LC (say 1% Honey Solution) When should I transfer from the LC to Grain?
I want to make sure that I do it at a time that the Mycelium is good and strong and not coming to teh end of its life cycle.
So far I have not had a great deal of success with LC. I have made my LC by just adding spres to a 1% Honey solution and I see some Myc in the jars but when I take this to grain, I have not had a much growth.
I am not getting as much trich as I use to when I used a SAB. I am now doing everything in a LFH that I built.
Thanks for your help.
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,387
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Re: When is the best time to use a LC to inoculate Grains? [Re: GermanShaman]
#27770173 - 05/09/22 05:52 PM (2 years, 8 months ago) |
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Anytime after it's colonized is good.
I have come to the conclusion that the best way to use LC, in my experience, is to not shake it or mix it around after inoculation.
Just use enough to wet the rim of bag or jar all the way around until the LC reaches the bottom of the grain. The LC goes so much farther and grows faster this way vs shaking a ton of it on the grains. And you see the growth very clearly and can tell if shit's going south right away.
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GermanShaman
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Registered: 01/05/18
Posts: 343
Last seen: 10 months, 28 days
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Re: When is the best time to use a LC to inoculate Grains? [Re: sandman420]
#27773571 - 05/12/22 07:07 AM (2 years, 8 months ago) |
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Thanks for that Sandman. I always inject the culture and then shake the jar to coat as much grain as possible and then let the jar sit for a few weeks.
I only shake the jar once it is fully colonized and I am going to bulk. I have not had much success with shaking jars that are partially colonized.
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,387
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Re: When is the best time to use a LC to inoculate Grains? [Re: GermanShaman]
#27773681 - 05/12/22 09:11 AM (2 years, 8 months ago) |
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Losing a jar after a shake means you have underlying bacteria issues. You may need to sterilize your grain longer or purge the air longer when using the pressure cooker.
The bacteria endospores would be the hardest thing to kill with sterilizing so partial success in sterilization leaves just the bacteria.
Edited by sandman420 (05/12/22 09:13 AM)
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normalperson
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Registered: 10/31/19
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Re: When is the best time to use a LC to inoculate Grains? [Re: GermanShaman]
#27773710 - 05/12/22 09:42 AM (2 years, 8 months ago) |
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Quote:
GermanShaman said: I have made my LC by just adding spres to a 1% Honey solution
It's quite likely that you have never had a clean LC. Put the spores to agar first, transfer to new plates until clean, and then add agar wedges to your broth.dont do only one jar, inoculate a few and deliberately contaminat one, it'll help you recognize a bad culture. Ime, once a inoculated grain jar starts showing new myc growth shaking it speeds up colonization. Shaking helps insure all of the grains are colonized.
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