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Offlinechaoscity
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Simple grain jar question
    #27770059 - 05/09/22 04:40 PM (2 years, 8 months ago)

I inoculated some wbs on 4/20. it still hasn't grown not even 1 percent just took over a few of the grains. I used agar, can I use more agar to help speed it up?

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OfflineHuskies
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Re: Simple grain jar question [Re: chaoscity]
    #27770069 - 05/09/22 04:47 PM (2 years, 8 months ago)

got pics of the plate?


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Offlinechaoscity
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Re: Simple grain jar question [Re: Huskies]
    #27770090 - 05/09/22 04:55 PM (2 years, 8 months ago)


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InvisibleMycelium Juice
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Re: Simple grain jar question [Re: chaoscity]
    #27770212 - 05/09/22 06:21 PM (2 years, 8 months ago)

Something ain’t right.  How did you hydrate your wbs?
  Might as well give sterilization process too.

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OfflineHuskies
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Re: Simple grain jar question [Re: chaoscity]
    #27770213 - 05/09/22 06:22 PM (2 years, 8 months ago)

was it spores to agar? did you make multiple transfer? best to do 3 transfer of mycelium to make sure there are no bacteria or mold on there.

I'd multiply that agar, and toss the jar. Don't try to salvage doomed endeavors in this hobby.


--------------------
I call them Huskies cause they Mush!


Instant Pot Agar
Gnocchi Water Agar
Let's Talk Cowtek

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Offlinechaoscity
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Re: Simple grain jar question [Re: Huskies]
    #27770947 - 05/10/22 09:46 AM (2 years, 8 months ago)

Quote:

Mycelium Juice said:
Something ain’t right.  How did you hydrate your wbs?
  Might as well give sterilization process too.




I used feelin funny's tek - soaked 24 hours, picked out the floaters, rinsed a lot.. A LOT. boiled water stirred, pressure cooked, then let it dry. Did everything in the tek step by step.


Quote:

Huskies said:
was it spores to agar? did you make multiple transfer? best to do 3 transfer of mycelium to make sure there are no bacteria or mold on there.

I'd multiply that agar, and toss the jar. Don't try to salvage doomed endeavors in this hobby.




it was spore syringe to agar, why is it doomed?

Edited by chaoscity (05/10/22 09:51 AM)

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Offlinechaoscity
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Re: Simple grain jar question [Re: chaoscity]
    #27771172 - 05/10/22 12:52 PM (2 years, 8 months ago)

So I checked again, apparently one is now about 10 percent!

I think I made a mistake... I misted one of the jars about 3 times to see if that makes a difference since I thought it could be too dry. Let me know if this was a bad idea.. I have 5 1/2 jars total so hopefully if the other ones colinize (I'm guessing they should since 1 is starting to} there will be enough to salvage a small tub?

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Offlinedyel
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Re: Simple grain jar question [Re: chaoscity] * 1
    #27771190 - 05/10/22 12:59 PM (2 years, 8 months ago)

you mean you opened the jar and misted it? if so throw it away

edit: don't throw away the container, just the content container is perfectly reusable

Edited by dyel (05/10/22 01:00 PM)

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Offlinechaoscity
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Re: Simple grain jar question [Re: dyel]
    #27771224 - 05/10/22 01:20 PM (2 years, 8 months ago)

thought that was a mistake, thanks

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InvisibleMTZ
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Re: Simple grain jar question [Re: dyel]
    #27771225 - 05/10/22 01:20 PM (2 years, 8 months ago)

Sounds like he PC'ed it before putting the grain in jars too. Unless it was a typo.


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Rehears your death every morning and night. Only when you constantly live as though already a corpse will you find freedom in the martial way, and fulfill your duties without fault throughout your life. ~ The Book Of Samurai
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Offlinechaoscity
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Re: Simple grain jar question [Re: MTZ]
    #27771287 - 05/10/22 02:19 PM (2 years, 8 months ago)

Quote:

MTZ said:
Sounds like he PC'ed it before putting the grain in jars too. Unless it was a typo.



No I didn't was a typo I meant to put after.

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OfflineHuskies
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Re: Simple grain jar question [Re: chaoscity] * 1
    #27772276 - 05/11/22 05:44 AM (2 years, 8 months ago)



--------------------
I call them Huskies cause they Mush!


Instant Pot Agar
Gnocchi Water Agar
Let's Talk Cowtek

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