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InvisibleLotKid
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Re: Mycology question...kind of technical question..... [Re: B Traven]
    #27773793 - 05/12/22 10:33 AM (2 years, 8 months ago)

:whathesaid:


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OfflineSockadin
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Re: Mycology question...kind of technical question..... [Re: LotKid]
    #27773818 - 05/12/22 10:50 AM (2 years, 8 months ago)

Popcorn kernels tend to expand in the microwave I wonder if that works with Spores as well?

:shrug:

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OnlineDERRAYLD
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Re: Mycology question...kind of technical question..... [Re: Sockadin]
    #27773860 - 05/12/22 11:28 AM (2 years, 8 months ago)

Maybe if you add butter!

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OfflineRotnpins
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Re: Mycology question...kind of technical question..... [Re: DERRAYLD]
    #27773896 - 05/12/22 11:50 AM (2 years, 8 months ago)

Quote:

DERRAYLD said:
Maybe if you add butter!




:popcorn:Dont forget to add a dash of salt, or seasoning of your choice :popcorn:

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InvisibleQuantumReality
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Re: Mycology question...kind of technical question..... [Re: LotKid]
    #27773953 - 05/12/22 12:20 PM (2 years, 8 months ago)

Quote:

LotKid said:
If i ate my memberberries then i think the conversion rate is something like almost 5 psi per ML. So the bigger the syringe the more psi it will tolerate. I think. Pretty sure i heard a scientist say it one time.




Might be a stupid question, but what about using a clamp instead of a rubber band/hair tie? :shrug:
I've got super old spores, old enough to drive a car kinda old, that I am trying to hydrate.

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InvisibleLotKid
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Re: Mycology question...kind of technical question..... [Re: QuantumReality]
    #27773962 - 05/12/22 12:24 PM (2 years, 8 months ago)

Quote:

Rotnpins said:
Quote:

DERRAYLD said:
Maybe if you add butter!




:popcorn:Dont forget to add a dash of salt, or seasoning of your choice :popcorn:





Quote:

QuantumReality said:
Quote:

LotKid said:
If i ate my memberberries then i think the conversion rate is something like almost 5 psi per ML. So the bigger the syringe the more psi it will tolerate. I think. Pretty sure i heard a scientist say it one time.




Might be a stupid question, but what about using a clamp instead of a rubber band/hair tie? :shrug:
I've got super old spores, old enough to drive a car kinda old, that I am trying to hydrate.



i was being sarcastic.  and i dont think those will work either. ppl have tried the rubber band method and.... myth busted.


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OfflineB Traven
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Re: Mycology question...kind of technical question..... [Re: LotKid]
    #27773994 - 05/12/22 12:41 PM (2 years, 8 months ago)

Yah, you'd need to apply pressure to the actual solution, like the way that a pressure washer works, or some sort of hyperbaric chamber, and not just squeeze the outside of the container.

But there's still no reason to think that it would make much of a difference in germination terms, and even if it did, it would be a pointless amount of time saved for the effort involved. If you don't have a few weeks to see if old spores will germinate, then you don't have time to grow mushrooms...


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InvisibleQuantumReality
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Re: Mycology question...kind of technical question..... [Re: B Traven]
    #27774006 - 05/12/22 12:50 PM (2 years, 8 months ago)

Yeah this is quite true.

I remember RR posting a tek once or something with a jar that had a lid rigged up with a one way tyre valve and a pressure meter, kept at 5 or so psi to force hydrate the spores.

On my behalf, it's not the time factor I've got plenty of that, I'm just seeing no signs of life on agar after weeks so I'm getting desperate. It's easy to obtain new spores. It's the canning of a vast library of non-viable spores that's breaking my heart.

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OfflineB Traven
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Re: Mycology question...kind of technical question..... [Re: QuantumReality]
    #27774030 - 05/12/22 01:10 PM (2 years, 8 months ago)

Quote:

QuantumReality said:
Yeah this is quite true.

I remember RR posting a tek once or something with a jar that had a lid rigged up with a one way tyre valve and a pressure meter, kept at 5 or so psi to force hydrate the spores.

On my behalf, it's not the time factor I've got plenty of that, I'm just seeing no signs of life on agar after weeks so I'm getting desperate. It's easy to obtain new spores. It's the canning of a vast library of non-viable spores that's breaking my heart.




Ok, I could see that 5 psi setup maybe helping a bit with really old spores...maybe emulsifiers would help, too? (longbow, 9 months is not very old at all)

I've had pretty good luck quenching my inoculating loop in sterilized water before swiping old prints.


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InvisibleQuantumReality
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Re: Mycology question...kind of technical question..... [Re: B Traven]
    #27774062 - 05/12/22 01:32 PM (2 years, 8 months ago)

Emulsification, not a bad idea :strokebeard:

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OfflineA.k.aM
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Re: Mycology question...kind of technical question..... [Re: Pastywhyte] * 1
    #27776827 - 05/14/22 11:44 AM (2 years, 8 months ago)

Quote:

Pastywhyte said:
Instantly you say?





Yep, once you get the rubber bands on tight enough it squeezes the mycelium out of the spore.


:fingerpistol:


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LAGM2020

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InvisiblePastywhyteMDiscord
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Re: Mycology question...kind of technical question..... [Re: A.k.a]
    #27776879 - 05/14/22 12:31 PM (2 years, 8 months ago)

Quote:

A.k.a said:
Quote:

Pastywhyte said:
Instantly you say?





Yep, once you get the rubber bands on tight enough it squeezes the mycelium out of the spore.


:fingerpistol:




:knockmeonmyass::kittylaugh:

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InvisibleGoatrider
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Re: Mycology question...kind of technical question..... [Re: A.k.a] * 1
    #27777738 - 05/15/22 03:07 AM (2 years, 7 months ago)

Quote:

A.k.a said:
Yep, once you get the rubber bands on tight enough it squeezes the mycelium out of the spore.





Absolutely agreed.

I´m on a tek with this.
Once i accidently injected spores to my bike tires.
I thought o.k... the coat reads 65psi max,
so not bad at all.
After a few weeks there were massive shrooms all around the tires.
Made a quick bike tour for easy harvest, done.

        :cookiemonster:


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