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CatsLoveHouseMusic
SpeakerFreaker



Registered: 11/03/18
Posts: 1,085
Last seen: 2 years, 6 months
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Favorite LC tek or tips? (I have very good success with agar, wanted to try LC instead of G2G.)
#27767984 - 05/07/22 10:40 PM (2 years, 8 months ago) |
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So I do very very well with agar. Either G2G or LC would be the best way to get boxes going, and i’m thinking I might do LC…. and try some G2G.
What are your favorite teks for LC? Any suggestions you find crucial when doing LC? I truly appreciate your advice guys!
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MysticMycologist
Dirt Sherpa



Registered: 10/14/21
Posts: 1,755
Loc: seeking samadhi
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Re: Favorite LC tek or tips? (I have very good success with agar, wanted to try LC instead of G2G.) [Re: CatsLoveHouseMusic]
#27767999 - 05/07/22 10:51 PM (2 years, 8 months ago) |
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Josex’s No Mercy. Hands down for LC. Liquid culture can be a real pain to make clean. Using the smallest transfer possible really helps to exclude contamination a lot.
Oh, and always test your LC on agar before using it on a bunch of grain. Made the mistake of blindly trusting my LC once, and ruined a whole batch of grain jars quick.
-------------------- Two eyes to look, One eye to see.
Prying open my third eye

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CatsLoveHouseMusic
SpeakerFreaker



Registered: 11/03/18
Posts: 1,085
Last seen: 2 years, 6 months
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Re: Favorite LC tek or tips? (I have very good success with agar, wanted to try LC instead of G2G.) [Re: MysticMycologist]
#27768020 - 05/07/22 11:04 PM (2 years, 8 months ago) |
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Is this the tek you’re talking about? https://www.shroomery.org/forums/showflat.php/Number/24740168/fpart/1/vc/1
Anything you would say is crucial when making LC? I havnt had a contam on agar in the last like 60+ plates, so am doing something right.
I always do a bunch of research on something if it’s important yet kinda difficult. Want to make sure I start off correctly! I do appreciate your time bud.
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MysticMycologist
Dirt Sherpa



Registered: 10/14/21
Posts: 1,755
Loc: seeking samadhi
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Re: Favorite LC tek or tips? (I have very good success with agar, wanted to try LC instead of G2G.) [Re: CatsLoveHouseMusic]
#27768340 - 05/08/22 09:04 AM (2 years, 8 months ago) |
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That’s the one
-------------------- Two eyes to look, One eye to see.
Prying open my third eye

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CatsLoveHouseMusic
SpeakerFreaker



Registered: 11/03/18
Posts: 1,085
Last seen: 2 years, 6 months
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Re: Favorite LC tek or tips? (I have very good success with agar, wanted to try LC instead of G2G.) [Re: MysticMycologist]
#27768598 - 05/08/22 11:51 AM (2 years, 8 months ago) |
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This is a lot easier thern i thought, fyi for whomever reading
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nerdintheshell



Registered: 09/11/21
Posts: 95
Loc: 'MURICA!
Last seen: 1 month, 22 days
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Re: Favorite LC tek or tips? (I have very good success with agar, wanted to try LC instead of G2G.) [Re: CatsLoveHouseMusic]
#27768660 - 05/08/22 12:58 PM (2 years, 8 months ago) |
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Josex poke is great for inoculating lc. Only additive that I think is worth a damn is peptone. Peptone really seems to increase growth ime. Also semi solid lc is the shiz.
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