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Offlinemud1
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Registered: 01/05/22
Posts: 46
Last seen: 2 years, 14 days
Space efficient experiment tek for cloning?
    #27765902 - 05/06/22 12:29 PM (2 years, 8 months ago)

I'm thinking of doing an amateur experiment to get the best clones from MSS.  My plan is to do either:
1. Innoc ~5 agar plates with MSS
2. For each agar plate, isolate another ~5 plates with good looking growth
3. Continue with those until I have an isolated strain
4. Fruit and clone the best ones


Or:
1. Just fruit ~10 MSS
2. Clone the best fruits into agar (~3 plates per clone)
3. Grow them and keep track of which agar clone produced the best fruits

With both setups, the built-in redundancy should yield some good results even if the success rate is low.  But the issue is both paths will need like 20+ fruiting chambers.  Is there a space efficient container I can use?  I was considering PF Tek as a possible solution due to the small cake size but was wondering if anyone else here had other container ideas

Thanks!

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OfflineSirPsycho
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I'm a teapot User Gallery


Registered: 01/01/20
Posts: 7,340
Loc: Rent free in your head
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Trusted Cultivator
Re: Space efficient experiment tek for cloning? [Re: mud1]
    #27765936 - 05/06/22 12:57 PM (2 years, 8 months ago)



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Offlinemud1
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Registered: 01/05/22
Posts: 46
Last seen: 2 years, 14 days
Re: Space efficient experiment tek for cloning? [Re: SirPsycho]
    #27766310 - 05/06/22 05:30 PM (2 years, 8 months ago)

Looks good, thanks!

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OfflineB Traven
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Registered: 03/10/20
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Re: Space efficient experiment tek for cloning? [Re: mud1] * 1
    #27766549 - 05/06/22 08:39 PM (2 years, 8 months ago)

I'm mainly just replying to bookmark those links....

But another layer that could save you space would be to just pump agar plates and keep running transfers, then let them all fully colonize and watch for pins to clone. No, you wouldn't have much info about how they'd perform until you grew them out, but you could at least start- in bottles or whatever you settled on as the space-saving fruiting test- with cleaned-up clone lines that are probably eager pinners.


--------------------
Beware of advice- even this.

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