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QuantumReality
Mycopath 🗡



Registered: 05/20/07
Posts: 3,204
Loc: BoobyTraps
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Can I get 16yo spores to germinate? (Ancient Spore Revival) 1
#27765764 - 05/06/22 10:19 AM (2 years, 8 months ago) |
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So basically I found a stack of prints (most dating back 16 or so years) that were kept in an insulated bag in my garage. Is there any chance at reviving some of these..? What are the best Tips to get some cube spores to germinate? I gotta say, I'm hopeful. Would be a shame for this little library of spores to be of no good 
TIA
QR
Edited by QuantumReality (05/14/22 04:30 PM)
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RedNeckBow
Sporulatting



Registered: 02/23/22
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Re: Can I get 16yo spores to germinate? [Re: QuantumReality] 1
#27765768 - 05/06/22 10:24 AM (2 years, 8 months ago) |
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No way to really know until you put them to agar and find out for yourself.
Hydration by pressure is an option.
I would just get a fresh print anyways.
-------------------- You know what's ironic.
Gettingg slammed in the head with an encyclopedia and loosing consciousness.
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Tight Lunchbox
Drunk cat


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Re: Can I get 16yo spores to germinate? [Re: QuantumReality] 1
#27765773 - 05/06/22 10:28 AM (2 years, 8 months ago) |
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I wouldn't expect much from spores that old. Try germinating them on softer agar. That's the only trick I know of.
-------------------- "it's all a joke between mom contractions and coffin fittings"
The most useful tool for noobs
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Goatrider
Rhythm Guitarist



Registered: 04/08/20
Posts: 4,801
Loc: Germany
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Re: Can I get 16yo spores to germinate? [Re: QuantumReality] 7
#27765781 - 05/06/22 10:31 AM (2 years, 8 months ago) |
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As usual with old spores, i´d first hydrate them for a while. Make some sterile water. Scrape spores into a sterile shotglas, shoot sterile water in, mix up a bit, and load the syringe. Let it sit at room temp for a few weeks to let them hydrate properly.
For germination i always recommend this. I promise you: Spores that won´t germinate on brf pucks aren´t viable. Brf is that damn nutritive and a beast of a weapon against bacteria. After germination you can transfer to agar for further clean up. Often you see pins on brf pucks, so you could even clone right from there.
Hope that helped a bit 
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WellDuYaPunk
Being



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Re: Can I get 16yo spores to germinate? [Re: Goatrider] 1
#27766767 - 05/07/22 12:02 AM (2 years, 8 months ago) |
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Tracking this. I believe I saw an older post here somewhere of someone getting 10 year old spores to germinate.
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QuantumReality
Mycopath 🗡



Registered: 05/20/07
Posts: 3,204
Loc: BoobyTraps
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Re: Can I get 16yo spores to germinate? [Re: WellDuYaPunk]
#27767227 - 05/07/22 11:02 AM (2 years, 8 months ago) |
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Thank you, I will try all of the above.
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SwabMarley
Twisted Metal Head



Registered: 12/07/20
Posts: 1,571
Loc: Drunken Stupor
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Yeah I’m curious to find out whether these are still viable. I second what Goatrider says, hydration and BRF puck. Keep us posted bud!
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QuantumReality
Mycopath 🗡



Registered: 05/20/07
Posts: 3,204
Loc: BoobyTraps
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Re: Can I get 16yo spores to germinate? [Re: SwabMarley]
#27768720 - 05/08/22 01:54 PM (2 years, 8 months ago) |
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Ok, so I'm playing with 12 strains of 'ancient cubensis spores'. I've made x5 soft agar plates with MDK, Cambo, Arg, B+, PFC. I've noc'd x5 lots of LC's (4% honey) with the same spores that went on agar. And lastly I've made x7 spore syringes with GT, BanPhangKa, BurmeseYangoon, EQ, KSSS, PE & Malaysian.
All of the LC and MS will incubate at 77-80F for a few weeks then will test on agar and BRF.
Would love to know how to force hydrate via pressure. Obviously it would have to be cold pressure. I believe I saw a contraption once from RR where he used a tyre valve and a pressure meter on a jar lid screwed to a jar and kept it at about 5psi. Cant remember for how long or if it was a tek he posted...
Anyways, I'll post some updates soon enough. Fingers crossed.
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A.k.a
Stranger



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Re: Can I get 16yo spores to germinate? [Re: QuantumReality] 2
#27768746 - 05/08/22 02:14 PM (2 years, 8 months ago) |
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Just wrap a rubber band from top to bottom so it’s pulling the plunger down, pretty sure that’s how people force hydrate.
Definitely curious, I’m sure with that many prints something will go off.
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LAGM2020     
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QuantumReality
Mycopath 🗡



Registered: 05/20/07
Posts: 3,204
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Re: Can I get 16yo spores to germinate? [Re: A.k.a]
#27771060 - 05/10/22 11:45 AM (2 years, 8 months ago) |
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Quote:
A.k.a said: Just wrap a rubber band from top to bottom so it’s pulling the plunger down, pretty sure that’s how people force hydrate.
Legendary
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WellDuYaPunk
Being



Registered: 01/23/22
Posts: 147
Loc: h ness monster
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Any action yet?
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Rotnpins
🤮 Rotten-Pins 🍄



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SwabMarley
Twisted Metal Head



Registered: 12/07/20
Posts: 1,571
Loc: Drunken Stupor
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Re: Can I get 16yo spores to germinate? [Re: Rotnpins]
#27775432 - 05/13/22 10:54 AM (2 years, 8 months ago) |
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QuantumReality
Mycopath 🗡



Registered: 05/20/07
Posts: 3,204
Loc: BoobyTraps
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Re: Can I get 16yo spores to germinate? [Re: WellDuYaPunk]
#27775492 - 05/13/22 11:42 AM (2 years, 8 months ago) |
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Nah, nothing yet 
The MS syringes are 30-60cc capacity so I will be testing plates weekly. I nocd up some plates last night so will keep any progress posted.
Also considering making one of these
RogerRabbit said: Here's the pressure chamber I built during the Redboy experiments to help hydrate old spores. Glass jars are not designed to hold pressure, so I was afraid to ever go over 5 to 7 psi with it. RR
Found here: https://www.shroomery.org/forums/showflat.php/Number/11063684/fpart/all
Looks like both fun & a pain in the ass to make 😂 just how I like it
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SwabMarley
Twisted Metal Head



Registered: 12/07/20
Posts: 1,571
Loc: Drunken Stupor
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Quote:
QuantumReality said: Nah, nothing yet 
The MS syringes are 30-60cc capacity so I will be testing plates weekly. I nocd up some plates last night so will keep any progress posted.
Also considering making one of these
RogerRabbit said: Here's the pressure chamber I built during the Redboy experiments to help hydrate old spores. Glass jars are not designed to hold pressure, so I was afraid to ever go over 5 to 7 psi with it. RR
Found here: https://www.shroomery.org/forums/showflat.php/Number/11063684/fpart/all
Looks like both fun & a pain in the ass to make 😂 just how I like it</font></font>
Although RR is the fuckin man! This post is from 12 years ago! I like to compare mushcult to computers or mobile (cell) phones. In the past 10 years alone they’ve come on leaps and bounds. So information from 12 years ago May as well be from 30 years ago.
Or something.
Probably.
Drunk.
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QuantumReality
Mycopath 🗡



Registered: 05/20/07
Posts: 3,204
Loc: BoobyTraps
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Re: Can I get 16yo spores to germinate? [Re: SwabMarley]
#27775668 - 05/13/22 01:58 PM (2 years, 8 months ago) |
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Quote:
Myc Hunt said: I like to compare mushcult to computers or mobile (cell) phones. In the past 10 years alone they’ve come on leaps and bounds. So information from 12 years ago May as well be from 30 years ago. </font>
nice analogy. You are probably right on that one. It definitely seems that way, since coming back into the game, much has changed.
There are people saying to use the syringe tek and then others who say the tek has been debunked and it doesnt work. Some say spores will germinate at 0.1 psi others say a phenomenal amount greater, I just want to hydrate these spores the best way really. I'm not gonna lie some of the spores I just worked with while some were easy to scrape of a print - others flaked off. The kind of flake you think "what am I even doing here?"
Nonetheless I am quite hopeful
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Tight Lunchbox
Drunk cat


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You might make some brf jars while you're at it. It can't hurt anything.
-------------------- "it's all a joke between mom contractions and coffin fittings"
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A.k.a
Stranger



Registered: 10/27/19
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That’s a good idea too.
Idk about old spores but with pans sometimes spores won’t want to grow on agar but if you put them to grain they pop off no problem.
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LAGM2020     
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QuantumReality
Mycopath 🗡



Registered: 05/20/07
Posts: 3,204
Loc: BoobyTraps
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Re: Can I get 16yo spores to germinate? [Re: A.k.a]
#27776452 - 05/14/22 04:02 AM (2 years, 8 months ago) |
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Quote:
Tight Lunchbox said: You might make some brf jars while you're at it. It can't hurt anything.
Quote:
A.k.a said: That’s a good idea too.
Idk about old spores but with pans sometimes spores won’t want to grow on agar but if you put them to grain they pop off no problem.
Yes some brf jars will be nocd up with MS and LC. How long do you guys think I should hydrate the spore for? I was thinking about 3 weeks min? 
Quote:
A.k.a said: Just wrap a rubber band from top to bottom so it’s pulling the plunger down, pretty sure that’s how people force hydrate.
What do think about a clamping of the syringe, rather than a rubber band? Surely the increased pressure would help?
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A.k.a
Stranger



Registered: 10/27/19
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Honestly I’ve never messed with it myself so I’m just going off what I’ve seen here in the past.
Anything to keep pressure on the plunger should work.
I’d try knocking up a couple things after a week, but waiting longer shouldn’t hurt.
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LAGM2020     
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