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Offlinefranksterling
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Registered: 07/31/14
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spores to agar - how to continue?
    #27762552 - 05/04/22 09:30 AM (2 years, 8 months ago)

Hi,
I inoculated a couple of petri dishes with spores of a syringe and can see mycelium forming on some of them.
From my understanding it makes sense to select some and transferr to new agar plates a couple of times to get the ideal bit of it.
Now lets say I have 5 dishes of agar with mycelium - where do I take the parts from to transfer to the next dishes?
Do I look for the dish that grows fastest and take just of this and transfer to new dishes or do I take of several dishes?
Also which parts of the original dishes are the ones I should take to the next dishes?
As I obviously did not had the chance to evaluate the fruits I am wondering what indicators there are to select which mycelium is worth replicating. Also what is a realistic number of plates for the second generation? How often should I repeat before going to rye?
Back in the days I used to take a pice of the best fruit I had in a growkit but this is my first time using spores so I don't really know how to choose.
Also I am wondering why not to take small jars instead of petri dishes. The cost roughly the same, I could sterilize the agar inside and don't have to deal with parafilm.
Thanks for helping :smile:

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Offlinedyel
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Registered: 10/15/21
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Re: spores to agar - how to continue? [Re: franksterling]
    #27762579 - 05/04/22 09:47 AM (2 years, 8 months ago)

those are a lot of questions, for starters post pics of your plates so people can see and point to best transfer locations

and yes you can use almost anything as a no-pour as long it seals and have decent visibility, i use all kind of stuff from ikea pp containers to empty tomato paste glass ones

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Offlinefranksterling
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Registered: 07/31/14
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Re: spores to agar - how to continue? [Re: dyel]
    #27762616 - 05/04/22 10:09 AM (2 years, 8 months ago)

Thanks for your fast answer.
So far the mycelium is still very small (1 cm diameter isles).
I just thought I gonna ask before so I dont miss the right time to go on.
Might post some photos once the mycelium has developed a bit further

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