Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   PhytoExtractum Kratom Powder for Sale   Kraken Kratom Red Vein Kratom   North Spore Bulk Substrate   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: < Back | 1 | 2 | 3 | 4 | Next >  [ show all ]
Offlinesmalltalk_canceled
Babnik
 User Gallery

Registered: 07/13/20
Posts: 3,174
Last seen: 16 hours, 46 minutes
Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27750336 - 04/25/22 07:43 AM (2 years, 8 months ago)

The problem with wet grains, is that sometimes the wet colonized grain jars will be so bacterial that they moment you spawn them, the trich shows up long before a first flush can compete.

Ive lost 200 subs to that shit when I was starting out, easily.
Now I dont even bother knocking up jars like that.

The people warning against wet grain are trying to help you avoid loss and pain


--------------------
Willpower is the one true virtue


Extras: Filter Print Post Top
OfflineUnbornCaius
Hobbyist
 User Gallery

Registered: 04/20/22
Posts: 45
Last seen: 2 years, 7 months
Re: Spores to Mycelium with Grain in 2 days [Re: smalltalk_canceled]
    #27750424 - 04/25/22 09:22 AM (2 years, 8 months ago)

You’re probably right, and I’m not arguing against that at all. I would rather experience it for myself than to just go by the word of a stranger, even if everyone here knows more than me and has years of experience.

I could just follow what everyone says word for word, but if I did that I wouldn’t have experienced the failure on my own and I would be able to place blame. If I fail here I know it 100% was my fault.

I do appreciate all the advice here and I am listening, I just want to follow through with this to see exactly how contamination comes to happen, if it does, even though my goal is to find success.

After all, the master has made more mistakes than the novice has even tried.

In this stage of my journey I think I would learn more from making mistakes and following through than to just scrap what I have now, so that I can experience the mistake on my own and gain insight and experience from that in itself for next time.

I think the data I can acquire from every run at this stage is more important to me than avoiding mistakes, but that’s just me.

I have plenty of time and I’m not in any rush.

That being said I still accept criticism here and advice and I will look into that and use that for next time.

I already started documenting this run in a notebook and took pictures (I know I said I didn’t have pictures of the spore syringes, I technically do but the photo only shows the label and you can’t see the liquid or spores behind it)

Extras: Filter Print Post Top
OfflineUnbornCaius
Hobbyist
 User Gallery

Registered: 04/20/22
Posts: 45
Last seen: 2 years, 7 months
Re: Spores to Mycelium with Grain in 2 days [Re: smalltalk_canceled]
    #27750429 - 04/25/22 09:28 AM (2 years, 8 months ago)



This is the jar that is farthest along I think

By the way I just wiped the jar off with a Lysol wipe to clean my fingerprints off because they were obstructing the view with the flash on. So that’s why the outside my appear smudged or wet

Edited by UnbornCaius (04/25/22 09:31 AM)

Extras: Filter Print Post Top
OfflineDERRAYLD
Constructus
Male User Gallery

Registered: 05/13/02
Posts: 12,109
Loc: South Africa
Last seen: 7 hours, 26 minutes
Trusted Cultivator
OG Cultivator
Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27750431 - 04/25/22 09:29 AM (2 years, 8 months ago)

As much as it's good to learn from your mistakes it can also be incredibly demotivating, don't get too hung up on the lesson if it's going to cause you to lose interest in the hobby.

Extras: Filter Print Post Top
OfflineUnbornCaius
Hobbyist
 User Gallery

Registered: 04/20/22
Posts: 45
Last seen: 2 years, 7 months
Re: Spores to Mycelium with Grain in 2 days [Re: DERRAYLD]
    #27750439 - 04/25/22 09:39 AM (2 years, 8 months ago)

Anyway, I’ll just share my progress here and leave updates in case anyone is interested.

I appreciate the care everyone has for me maintaining an interest in this, and believe me mycology has been an interest of mine for about 5 years and I own and have read dozens of mycology and mushroom identification books, I’m on mushroom facebook groups and frequently go foraging for mushrooms in my area.

I strongly believe mushrooms can change and even save the world as Paul Stamets says.

I just haven’t had a lot of experience with cultivation because I’ve been so busy, but now I have time for it so I’m taking advantage of it.

I’m not worried about a failed grow crushing my spirits, I understand that’s just how things go if you make mistakes and I won’t take it too personally.

I think maybe the reason the spores germinated so quickly was maybe due to the source. I’ve been looking into it and the source seems to be highly appraised

Extras: Filter Print Post Top
Offlinedyel
Male User Gallery


Registered: 10/15/21
Posts: 920
Last seen: 3 months, 4 days
Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27750521 - 04/25/22 10:58 AM (2 years, 8 months ago)

Quote:

UnbornCaius said:
And the syringes may have been germinating already, but I looked as closely as I could at them beforehand to look for contamination or anything weird and all I saw was clear water and spores clumped together. I shook the syringes until they were separated enough and injected them. I’m barely a novice so I don’t know for certain but I’m pretty sure I didn’t see any mycelium growth yet.



you just might have hit jackpot with some Ronnie Coleman genetics and got hyper responders there :lol:
lightweight babe lightweight

good luck on your grow and keep us posted

Extras: Filter Print Post Top
OfflineUnbornCaius
Hobbyist
 User Gallery

Registered: 04/20/22
Posts: 45
Last seen: 2 years, 7 months
Re: Spores to Mycelium with Grain in 2 days [Re: dyel]
    #27758776 - 05/01/22 02:06 PM (2 years, 8 months ago)

Thanks Dyel :grin: I think you are right, the Texas ghost genetics seem to be extremely aggressive. The source I got it from seems to be highly reputable and passionate about mycology

It seems 12/12 jars are healthy and free of contamination as far as I can tell. The Texas ghost strain has grown a lot more aggressively than the pink Buffalo. Once the jars reached 30% colonization I beat them with a tennis ball to break them up and then allowed them to fully colonize which happened within a day or two after that.

The first jar to reach 100% was a Texas ghost jar which took 9 days total from spore. Right now there are two pink Buffalo jars that have not fully colonized yet but should be there within a couple of days I would imagine.

I used a local field aged straw and grass fed horse manure that I collected myself which were in nugget form, they were leeched and dried naturally on the pasture.

I mixed about 20% vermiculite and 5% gypsum with 9 quarts of crumbled manure, brought to field capacity, put the substrate into a black lab garbage bag, and then used a steam pasteurization chamber with the temp gauge stuck directly in the center of the bag which was suspended using a wire rack to keep it out of the collecting water.

I let the temp get to 150-160F and kept it there for an hour.

Once that was done I let it cool to about 80F and set up my mono tub, it has 6 total holes which are 2” diameter. The lower holes are 3” above the bottom of the tub.

I used a black lawn bag and set a liner after thoroughly sanitizing the inside of the tub, and put a layer of manure sub, then grain spawn. I continued this until I used about 4 quarts of grain.

Before I inoculated with grain spawn I took a moment to get a good sense of what the spawn smelled like, it was incredibly earthy and smelled like textbook mushroom smell. Really pleasant earthy smell in each of the jars, nothing smelled weird or off. The manure smelled like fresh soil on a cool summer night after a brief rain shower.

Now I will wait for the pink buffalo to finish colonizing their grain and wait for the fruiting chamber to colonize as well.

I’m hoping to get some good samples to agar plates from the fruits.

Here are a bunch of progress pictures since my last post.


Edited by UnbornCaius (05/02/22 12:00 AM)

Extras: Filter Print Post Top
Offlinedyel
Male User Gallery


Registered: 10/15/21
Posts: 920
Last seen: 3 months, 4 days
Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27759577 - 05/02/22 06:08 AM (2 years, 8 months ago)

I'm curious how fast will these be as LC or LI
ofc it is still on trial for yeald and potency before being named a winner
:popcorn:

Extras: Filter Print Post Top
OfflineUnbornCaius
Hobbyist
 User Gallery

Registered: 04/20/22
Posts: 45
Last seen: 2 years, 7 months
Re: Spores to Mycelium with Grain in 2 days [Re: dyel]
    #27760004 - 05/02/22 01:26 PM (2 years, 8 months ago)

I’m really curious as well.

I will keep the thread updated, I will probably take photos and make notes as I go like my last post and then make a longer post all at once with photos and chronology.

So it hasn’t even been 24 hours since I inoculated the bulk substrate with the grain and I already see mycelium growth coming through to the top of the manure layer.

Extras: Filter Print Post Top
Offline1Lowkey75
1LowKey
Male
Registered: 02/01/22
Posts: 1
Loc: USA
Last seen: 2 years, 4 months
Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27760542 - 05/02/22 07:59 PM (2 years, 8 months ago)

Looking great I love an underdog story !

Extras: Filter Print Post Top
OfflinePGwojowski
Stranger

Registered: 05/30/21
Posts: 32
Last seen: 4 months, 20 days
Re: Spores to Mycelium with Grain in 2 days [Re: 1Lowkey75]
    #27760823 - 05/03/22 12:35 AM (2 years, 8 months ago)

Omg haha I love the heavy amount of doubters at the beginning of this thread spewing out notes from their rule books, telling you how death was imminent, your pep talks to yourself, and now I’m looking at many jars of colonized grain and a set monotub. You also are doing all of this from scratch with all of your own materials making your own bulk substrate. I hope you have a bountiful supply of mushrooms soon!

Extras: Filter Print Post Top
OfflineDERRAYLD
Constructus
Male User Gallery

Registered: 05/13/02
Posts: 12,109
Loc: South Africa
Last seen: 7 hours, 26 minutes
Trusted Cultivator
OG Cultivator
Re: Spores to Mycelium with Grain in 2 days [Re: PGwojowski] * 1
    #27760853 - 05/03/22 01:43 AM (2 years, 8 months ago)

Quote:

PGwojowski said:
Omg haha I love the heavy amount of doubters at the beginning of this thread spewing out notes from their rule books, telling you how death was imminent, your pep talks to yourself, and now I’m looking at many jars of colonized grain and a set monotub. You also are doing all of this from scratch with all of your own materials making your own bulk substrate. I hope you have a bountiful supply of mushrooms soon!




There's a healthy amount of doubters for a reason, those jars are all bacterial.
That doesn't mean he won't get fruit but it also means he may get nothing.

Extras: Filter Print Post Top
Offlinedyel
Male User Gallery


Registered: 10/15/21
Posts: 920
Last seen: 3 months, 4 days
Re: Spores to Mycelium with Grain in 2 days [Re: PGwojowski]
    #27760870 - 05/03/22 03:06 AM (2 years, 8 months ago)

I hope op gets to harvest but a few unwanted spores in the syringe and he could be raising a mold farm and believe me you don't want to be the one cleaning that shit.
what is recommended here is to have control over your grows (as much as possible with limited resources) and not following the spray and pray mentality
there is another place for that and it's called reddit i believe

Extras: Filter Print Post Top
OfflineUnbornCaius
Hobbyist
 User Gallery

Registered: 04/20/22
Posts: 45
Last seen: 2 years, 7 months
Re: Spores to Mycelium with Grain in 2 days [Re: dyel]
    #27761753 - 05/03/22 06:08 PM (2 years, 8 months ago)

I agree using the spore to grain method is not desirable and I didn’t really think about it when I was doing it, since I did it the first time I grew and had 100% success.

Now I know and certainly will do spore to agar beforehand to make sure, but let’s consider this thread my personal experiment.

Even if I have success I’ll understand it was luck, so I appreciate all the quality advice and everything.

I learned that spore to grain isn’t optimal because collecting spores is an inherently risky operation in terms of contamination, unless your entire grow room and spore print area are in the same room that has positive pressure, double filtered through a HEPA system, and there is a decontamination chamber before entry… and even then there is still risk of contamination especially if the substrate isn’t 100% sterilized through a double ended autoclave that goes through a wall into your grow room.

Basically none of that crossed my mind how the spore syringes were made, so now I know.. but that’s alright because making mistakes is all part of the learning experience and that’s what we have communities like this for, to share our experience and help eachother.

Edited by UnbornCaius (05/03/22 06:09 PM)

Extras: Filter Print Post Top
Offlinesmalltalk_canceled
Babnik
 User Gallery

Registered: 07/13/20
Posts: 3,174
Last seen: 16 hours, 46 minutes
Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27761778 - 05/03/22 06:31 PM (2 years, 8 months ago)

Exactly. Even if this works, later syringes may not be clean enough.
The proposition to go agar is about reliability, if one wants to throw the dice and be reliant on other people's work, spore solutions to grain is perfectly acceptable.


--------------------
Willpower is the one true virtue


Extras: Filter Print Post Top
OfflineUnbornCaius
Hobbyist
 User Gallery

Registered: 04/20/22
Posts: 45
Last seen: 2 years, 7 months
Re: Spores to Mycelium with Grain in 2 days [Re: smalltalk_canceled]
    #27767488 - 05/07/22 03:09 PM (2 years, 8 months ago)

Here is a small update,

I realized I had about a cc of spores left in each syringe, and I had a jar of grain left from both strains. I bought a 12 pack of 4oz jam jars that I used to make malt extract agar jars.

1.5 days ago I put ghost spores in 3 jars, pink Buffalo spores in 3 jars, I put a little piece of mycelium from ghost grains in 3 jars, and mycelium from pink Buffalo in 3 jars.

So far all of the grain to agar transfers have started growing onto the agar, no contamination visible.

None of the spore to agar jars have grown anything, I don’t see contam yet either.

The tubs seem to be growing really fast according to what I’ve seen in most grows.

The furthest colonized tub is about 70 quarts day 6 after spawn to bulk substrate and it is the ghost strain, a lot of rhizomorphic growth

The other tub is about 92 quarts day 4 after spawn to bulk, and it is the pink Buffalo strain, a lot of fluffy tomentose growth in comparison to the ghost tub

So I’ve been thinking about casing teks, I just read this book called “Conscious Cultivator: Psilocybe Edition” and there is something called a cheesecloth tek which seems really interesting. I looked it up and didn’t see really anything here on shroomery but I found some pretty unscientific ways of testing it other places.

I think this time I’m going to just do 50% peat moss 50% vermiculite, increase ph with lime to about 7.2-7.8 and then stabilize the ph with gypsum, but I do want to run tests on the cheesecloth casing when I get some clones and have fine tuned control of all the variables. That way I can make sure every variable except the casing is different, and I would run the experiment multiple times and track the data to compare the differences.

Anyway if anyone has any advice on a good casing tek I would be happy to hear it because this is my first time growing in a bulk substrate.

EDIT: I’ve decided to just run these mono tubs without a casing layer, since I don’t think I really need it, but I will be doing experiments on it later on in future grows.

By the way I will get pics of the agar jars when they look more interesting.

The photos of the tubs are kind of unclear because they’re taken behind the plastic. I don’t want to open the lids until they are ready for a casing layer, I don’t want to unnecessarily introduce contaminants when it isn’t 100% colonized, and I don’t want to release CO2 and humidity which I think may give mixed signals to the mycelium to start pinning. I’d rather make effective and efficient steps rather than poking my nose in the tub everyday, maybe it’s not that big of a deal but that’s just my noob opinion




Edited by UnbornCaius (05/07/22 09:10 PM)

Extras: Filter Print Post Top
OfflineUnbornCaius
Hobbyist
 User Gallery

Registered: 04/20/22
Posts: 45
Last seen: 2 years, 7 months
Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27767938 - 05/07/22 09:50 PM (2 years, 8 months ago)

Here are some of the grain to agar jars, and I decided to start fruiting the ghost tub since the mycelium looked about 80%-100% done colonizing


Edited by UnbornCaius (05/07/22 10:22 PM)

Extras: Filter Print Post Top
OfflineUnbornCaius
Hobbyist
 User Gallery

Registered: 04/20/22
Posts: 45
Last seen: 2 years, 7 months
Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27769818 - 05/09/22 01:39 PM (2 years, 8 months ago)

Day 8: Update of the tubs

Pink Buffalo still has tape over the holes until the sub colonized.
Still haven’t opened the lids, there is still a lot of light moisture in the tubs.


Extras: Filter Print Post Top
InvisibleAshtray161
SettledNomad
Other


Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
Re: Spores to Mycelium with Grain in 2 days [Re: fungusul]
    #27769848 - 05/09/22 02:02 PM (2 years, 8 months ago)

Quote:

fungusul said:
Welcome to Shroomery! :mushroom2:

:imo: Sorry. It has a great chance to be contaminated before 100% colonization given the amount of extra water and spore syringe directly to grains.

- Spore syringe directly to grains is a no-no because of notorious contamination rate.
- Gypsum is not required
- Coffee is not required
- Extra water increases the chances of contamination
- Good WBS teks FooMan WBS Prep or Reverse FooMan WBS Prep

Read Clean spawn checklist for the new grower to help you identify clean spawn.



:whathesaid:


--------------------
(You Know What Time It Is)
Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086:elmo:
"You never have to prove the fool a fool, just let them speak."
Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED.
Be Gay, Do Crime 161 1312

Extras: Filter Print Post Top
OfflineUnbornCaius
Hobbyist
 User Gallery

Registered: 04/20/22
Posts: 45
Last seen: 2 years, 7 months
Re: Spores to Mycelium with Grain in 2 days [Re: Ashtray161]
    #27770050 - 05/09/22 04:33 PM (2 years, 8 months ago)

I do have 6 seemingly healthy cultures from my grain spawn to agar with no visible contamination, and 6 MSS to agar that just started germinating. (I see some suspicious areas on the MSS-agar jars but not certain yet, I moved them in a separate area in isolation) I guess we will see what happens here.

I hope I can get fruits here, and I don’t see anything indicative of contamination yet but if it does pop up oh well not the end of the world.

Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3 | 4 | Next >  [ show all ]

Shop: OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   PhytoExtractum Kratom Powder for Sale   Kraken Kratom Red Vein Kratom   North Spore Bulk Substrate   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* Which spores are grain lovin?????? mushroom_man 1,161 3 11/16/02 11:26 AM
by Anonymous
* What Kills Spores/Mycelium BigBlackNinja 2,487 1 03/26/03 05:43 AM
by SixCee
* The ease of rye grain. Anonymous 14,728 19 06/03/16 03:10 PM
by Disco Shiny
* Re: Will H2O2 kill my spores???? PanTrop 1,835 5 02/02/00 05:51 PM
by vivid
* Would this work to innoculate grain?
( 1 2 all )
NiGGy 3,150 23 01/04/03 01:30 PM
by Skryllz
* can spores germinate in water? XOCHI 2,444 2 06/26/02 05:53 AM
by Mycelium
* Whats the point of grains? Fd3000 1,382 11 07/24/02 09:04 PM
by phrozendata
* NO MY SPORES WERE CONFISCATED KatatoniK 4,077 18 06/23/02 12:41 PM
by KatatoniK

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
3,047 topic views. 21 members, 99 guests and 66 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.009 seconds on 14 queries.