|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
|
Re: Why aren't my tubs fruiting?! [Re: Cosmos112]
#27753128 - 04/27/22 09:41 AM (2 years, 8 months ago) |
|
|
Don’t stress about rhizo vs tomentose, worry more about the vigour of the growth. How fast it grows. Is it looking strong or weak? When you do agar (or even brf paste) put spores to a few different plates and then choose the fastest/strongest looking candidates.
|
Cosmos112
Stargazer



Registered: 11/16/16
Posts: 100
Last seen: 1 year, 8 months
|
Re: Why aren't my tubs fruiting?! [Re: Cosmos112]
#27753146 - 04/27/22 09:48 AM (2 years, 8 months ago) |
|
|
Hmm, and you wouldn't think this kind of mishap would happen again? I mean, I really thought I had some healthy agar plates at hand and now I find out it's bacteria all along. I did some transfers yesterday and might post a few pics of what I'm dealing with,too. Maybe we can solve this enigma and make me a happy psychonaut once again 😍😂
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
|
Re: Why aren't my tubs fruiting?! [Re: Cosmos112]
#27753154 - 04/27/22 09:53 AM (2 years, 8 months ago) |
|
|
It could be the grains of course. How long did you sterilize them for? How long are you venting the PC before you build pressure? These are definitely factors.
|
Cosmos112
Stargazer



Registered: 11/16/16
Posts: 100
Last seen: 1 year, 8 months
|
Re: Why aren't my tubs fruiting?! [Re: Pastywhyte]
#27753163 - 04/27/22 10:00 AM (2 years, 8 months ago) |
|
|
I'm using an Instant Pot,which only keeps a pressure of 11 psi,so I pc two grain jars for 240 minutes,let the pressure drop on its own and immediately shake once they're cool enough to handle. Though I'm a newb at WBS/millet, it might be my prep thats off. My procedure is as following: Bring a pot of water to a boil,get it off the heat,add desired amount of millet,slap a lid on there and wait for 20 minutes. Drain and shake a little to help the water evaporate and after 30 minutes of cooling them down I load up my jars and sterilize as written up there.
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
|
Re: Why aren't my tubs fruiting?! [Re: Cosmos112]
#27753185 - 04/27/22 10:18 AM (2 years, 8 months ago) |
|
|
I don’t use or trust instapots myself. I won’t comment further on that. But I will suggest you try and get a proper PC if you can as it will remove some of the guess work.
|
Cosmos112
Stargazer



Registered: 11/16/16
Posts: 100
Last seen: 1 year, 8 months
|
Re: Why aren't my tubs fruiting?! [Re: Cosmos112]
#27753215 - 04/27/22 10:41 AM (2 years, 8 months ago) |
|
|
Maybe thats really the only viable solution to many of my problems, I shall look into getting myself a proper pressure cooker 😥
|
SharaVabdas



Registered: 03/23/22
Posts: 489
|
Re: Why aren't my tubs fruiting?! *DELETED* [Re: Cosmos112]
#27753360 - 04/27/22 12:47 PM (2 years, 8 months ago) |
|
|
Post deleted by SharaVabdas
Reason for deletion: Shroomery is full of bigots and I'm leaving.
|
Cosmos112
Stargazer



Registered: 11/16/16
Posts: 100
Last seen: 1 year, 8 months
|
Re: Why aren't my tubs fruiting?! [Re: SharaVabdas]
#27753382 - 04/27/22 01:22 PM (2 years, 8 months ago) |
|
|
Yeah, I'll just wait and see what happens. Clearly I'm overthinking a lot of things here and not a day goes by where I dont touch the tubs 🙈 Do you guys usually spawn your tubs and leave them alone?
|
PBJ710
Strangler


Registered: 07/05/19
Posts: 3,301
Last seen: 5 hours, 49 minutes
|
Re: Why aren't my tubs fruiting?! [Re: Cosmos112]
#27753385 - 04/27/22 01:27 PM (2 years, 8 months ago) |
|
|
Quote:
Cosmos112 said:Do you guys usually spawn your tubs and leave them alone?
100% of the time. I don't even look at them for the first week after spawning.
|
TheConfluence
Stranger



Registered: 12/13/21
Posts: 157
|
Re: Why aren't my tubs fruiting?! [Re: Pastywhyte]
#27753389 - 04/27/22 01:31 PM (2 years, 8 months ago) |
|
|
I use an instant too and it really does suck
-------------------- When two people dream the same dream, it ceases to be an illusion.
Philip K. Dick
Lies, Inc. (1984)
|
LSDChaos
TeamTrip



Registered: 05/19/16
Posts: 42
Last seen: 2 years, 8 months
|
Re: Why aren't my tubs fruiting?! [Re: Cosmos112] 1
#27753427 - 04/27/22 02:02 PM (2 years, 8 months ago) |
|
|
Looks like stroma, not sure if I can post a link to the site. So here's a copy/paste.
The good one — overlay and stroma are not contaminants. And there are chances to fix the problem.
The bad one — growing conditions for pinhead initiation have not been met. So, you might not get harvest at all!
Causes: ⛔️ Prolonged time for casing layer colonization. Fruiting conditions should be provided when 50-70% of the casing layer is covered with mycelium. Rhizomorph mycelium can stay in the incubator for a longer period.
⛔️ Too dry casing layer.
⛔️ Too wet casing layer.
⛔️ Low relative humidity in the GrowBox or Monotube (less than 90%).
⛔️ High carbon dioxide (CO2) concentration (above 600-800 ppm).
⛔️ High temperature (25°C or higher). Sharp temperature fluctuations.
⛔️ High evaporation rate. The humidity level in the growbox is high, but it is provided by evaporation from the casing layer instead of misting or using humidifier. This way mushroom cake dries out very quickly.
⛔️ High substrate acidity. To prevent substrate acidification, it is recommended to add buffering agents (limestone flour, dolomitic limestone flour, gypsum, chalk, marl, oyster shells) to the casing layer or secondary substrate for the monotube. Details are in Casing of magic mushrooms cakes
⛔️ Strain degeneration. Many strains from spores may be capable only for vegetative growth. Such mycelium can absorb nutrients, but is unable for pinning and fruit bodies development.
⛔️ Overlay stroma are not simply a disruption of just one fruiting parameter. It is often a complex of several factors:
high temperature + low RH + high CO2 = overlay low RH + high CO2 = overlay dry casing layer + high CO2 = overlay dry casing layer + low RH + high CO2 = overlay any of parameters + genetic changes = stroma If you don't decrease the temperature in the growbox/monotube, don't increase relative humidity (RH), don't provide oxygen regularly for mushrooms breathing, mycelium continue vegetative growth like before in the spawn jars and skip pinhead initiation process.
Edited by LSDChaos (04/27/22 02:04 PM)
|
LSDChaos
TeamTrip



Registered: 05/19/16
Posts: 42
Last seen: 2 years, 8 months
|
Re: Why aren't my tubs fruiting?! [Re: Cosmos112]
#27753451 - 04/27/22 02:18 PM (2 years, 8 months ago) |
|
|
Quote:
Cosmos112 said: Yeah, I'll just wait and see what happens. Clearly I'm overthinking a lot of things here and not a day goes by where I dont touch the tubs 🙈 Do you guys usually spawn your tubs and leave them alone?
I personally inspect everything every day, taking lots of notes I just never open anything until fully colonized. If for some reason I do open say a monotub its usually if I see 0 or very little signs of colonization after 3-5 days, but that's just to see if it has any odd smells. If it does I remove it from the grow room and wait to see what happens just to be safe.
|
Cosmos112
Stargazer



Registered: 11/16/16
Posts: 100
Last seen: 1 year, 8 months
|
Re: Why aren't my tubs fruiting?! [Re: LSDChaos]
#27753537 - 04/27/22 03:28 PM (2 years, 8 months ago) |
|
|
Holy smokes,thats a lot of information,I can't remember all of that 😱 Correct me if I'm wrong but isn't faster to directly fruit your tubs,since contaminations only occur due to funky spawn?
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
|
Re: Why aren't my tubs fruiting?! [Re: Cosmos112] 2
#27753539 - 04/27/22 03:30 PM (2 years, 8 months ago) |
|
|
I disagree with a lot of that information posted above and I always directly fruit at spawning. The reasons the gourmet industry does spawn runs in elevated CO2 do not apply to what we generally are doing when we are growing cubes.
For example stroma is incredibly rare and overlay isn’t a thing for cubensis. I’ve run 1000’s of tubs and only seen actual stroma like twice. I never once saw real overlay until I did Pans.
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
|
Re: Why aren't my tubs fruiting?! [Re: Pastywhyte]
#27753545 - 04/27/22 03:34 PM (2 years, 8 months ago) |
|
|
For example; dead dried out rubbery mycelium caused by poor conditions isn’t stroma, it’s dead dying rubbery mycelium caused by poor conditions. Personally I feel “stroma” is more of a reaction to bacteria and is at least somewhat genetically dependent. A person who was burnt in a fire isn’t the same as someone with a sunburn. Sunscreen isn’t a solution to a person who was burnt in a fire.
|
Cosmos112
Stargazer



Registered: 11/16/16
Posts: 100
Last seen: 1 year, 8 months
|
Re: Why aren't my tubs fruiting?! [Re: Pastywhyte]
#27753600 - 04/27/22 04:22 PM (2 years, 8 months ago) |
|
|
Well damn,thanks Pasty for seperating good from bad information, I shouldn't believe ALL of the advice given, even though it's meant in a positive manner 😅 So messing up the mycelium due to my inexperienced handling of the tubs is really whats going on here and the solution would be a couple of things: Check for clean spawn, dont touch the tubs after spawning to bulk and follow trusted teks to a T 🙈
|
SharaVabdas



Registered: 03/23/22
Posts: 489
|
Re: Why aren't my tubs fruiting?! *DELETED* [Re: Cosmos112]
#27754306 - 04/27/22 10:28 PM (2 years, 8 months ago) |
|
|
Post deleted by SharaVabdas
Reason for deletion: Shroomery is full of bigots and I'm leaving.
|
Cosmos112
Stargazer



Registered: 11/16/16
Posts: 100
Last seen: 1 year, 8 months
|
Re: Why aren't my tubs fruiting?! [Re: SharaVabdas] 1
#27754814 - 04/28/22 09:40 AM (2 years, 8 months ago) |
|
|
Seems like something I should get going, so I'm not too hung up on a potential loss.
Just bought four 33qt tubs which I'm probably gonna mod Pasty EzDial style for further grows (unless you guys got a different idea of course 😅).
Here is a picture of the current plate I'm growing from (it's terribly hard to take a nice picture so forgive me for the quality, but I swear there are filamentous strands reaching outwards in a tomentose manner)
|
Cosmos112
Stargazer



Registered: 11/16/16
Posts: 100
Last seen: 1 year, 8 months
|
Re: Why aren't my tubs fruiting?! [Re: Cosmos112]
#27756060 - 04/29/22 03:16 AM (2 years, 8 months ago) |
|
|
Sorry for bumping my own thread, I thought maybe someone can give me an opinion on my spawn jars,these have been inoculated with spore solution:
 I think they're looking pretty good, so technically I could rule out the bad spawn thingy 😅
|
DERRAYLD
Constructus


Registered: 05/13/02
Posts: 12,109
Loc: South Africa
Last seen: 8 hours, 1 minute
|
Re: Why aren't my tubs fruiting?! [Re: Cosmos112]
#27756061 - 04/29/22 03:18 AM (2 years, 8 months ago) |
|
|
Those don't look great, pretty sure I see some green on the left as well.
|
|