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OfflineDemetrius18
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Old LC and grain longevity viability experiment
    #27746693 - 04/22/22 12:04 PM (2 years, 8 months ago)

Recently Deme's toenail fungus has gained sentience and did a bad job keeping up with cultures, so there was a liquid culture(golden teacher, 1st attempt LC, rye berry water) that had been sitting at room temperature for at least 6 months.

Flowhood + face mask + disposable gloves + excessive iso alcohol spraying, many successful flushes (not first rodeo)


Rye berry jars (sfd) 4-5 months old still sealed from PC were the recipients, with 3 different golden teacher samples, 2 g2g & 1 LC.

The 2 grain cultures (also 6+ month) had a large pool of metabolites between the glass and shrunken (body/cake), one of them had already invitro started fruiting in the jar and the fruits already withered; the other had begun pinning but was stalled (overlay..?).

Both jars had the top of the cake scraped off and removed & were transferred g2g from viable myc under the surface
(seems to be successful a couple weeks in, just slow as the myc recovers and gets fuzzy again)

The jar the LC went into had been overpcooked, so the bottom half of the jar was a starchy mess/clump of burst grains (heaven for bacillus- or whatever you call that anaerobic bacteria baculum etc) but the top half (of the empty jar) was normal enough for a transfer, fastest growth of the 3 by a mile. Elaboration at end*


Will add pictures soon, forgot the exact day but let's say transfered at the start of April, all 3 done same time.

Just trying to add experience to the longevity and viability which may not be clearly known.


Some people say burst grains are a contaminate vector, I saw a good counterargument about how is it different in/out side of a grain w the skin being the only barrier as PC-ing heats the core sub anyway

Also grain drying out because of a long period of time is the main viability factor if the environment and process was sanitary/sterile IMO from what I've seen


*The LC has the fastest growth of the 3 but it looks clean, very rizomorphic growth thready myc, not like an ice cream cake, fast but not too fast, like a centimeter in the last 4-5 days, the 2 g2g are still recovering (just finished recovering today)

AND WILL POST PICS SOON GOTTA HATE WHEN THE STORY ISNT A PICTURE BOOK LOOOL


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OfflineDemetrius18
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Re: Old LC and grain longevity viability experiment [Re: Demetrius18]
    #27748015 - 04/23/22 11:42 AM (2 years, 8 months ago)

Added pictures, lol the grain is just tilted higher on the myc side, looks waaaay too full haha.

But everything personally looks clean on all 3 so pls anyone interested let me know if you believe differently (will transfer to agar when available to fully check contams)

Also 2 agar petris over a year old from cold storage have been reintroduced, for anyone interested how viable they are as it's recommended to transfer to a new plate twice a year.

2 pictures of g2g, and 5 shots of the LC transfer closeups etc




That's a sharpie line on the LC jar from 4-17 to track growth (not something inside jars for some angles), pics taken same day as this post.

All jars nocced same time, so all three the exact same age.


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Edited by Demetrius18 (04/23/22 11:48 AM)

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InvisibleSudoNimh
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Re: Old LC and grain longevity viability experiment [Re: Demetrius18]
    #27748508 - 04/23/22 06:34 PM (2 years, 8 months ago)

Nice! What was the recipe for your rye water LC?

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OfflineDemetrius18
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Re: Old LC and grain longevity viability experiment [Re: SudoNimh]
    #27748631 - 04/23/22 07:48 PM (2 years, 8 months ago)

Didn't use an actual recipe per say, just boiled water from the rye grain prep, it had gypsum in there too, pc-ed it with the other grain jars for 1.75 hours.

Saw somewhere on the threads that if you know the basic underlying process you shouldn't need a tek for LC, which is a load of shit tbh cuz teks are teks, but regardless just played it by ear to dabble in LC.

Started some gourmet stuff as well as old plates, several in LC, they seem to be doing well from what little can be seen, THAT recipe was honey water PC-ed for 15 minutes, was looking around and I believe 4% sugar agent is the ratio, you can use several syrups fructose etc as options, just not sucrose sugar (normal table sugar)

So a tablespoon of honey per 500-600ml of water, sorry for the exorbitant ratio size, PC for 15 minutes

LC can caramelize from too long in the sterilization heat, and less is better than too much, can't remember but I think 10% sugar content won't allow it (or anything) to grow (same concept as jams, why they're called preserves)


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InvisibleSudoNimh
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Re: Old LC and grain longevity viability experiment [Re: Demetrius18]
    #27757542 - 04/30/22 12:11 PM (2 years, 8 months ago)

Thank you! Rye berries are hard for me to find but I finally got my hands on a few pounds. I don't want to waste it by guesswork when I've only used millet and horse oats for years (if it ain't broke). Rye costs a lot more for me. Any varieties that particularly thrive on rye over others...?

And I agree with teks needing to be teks lol The whole "if you know, then you know" mentality is how we end up with tribal knowledge that we've lost the context to. It's an obsolete, elitist gatekeeping that I really don't subscribe to.

Recipes take the guesswork out for everyone... even if you just throw one together. I couldn't possibly know what ratio you used that was successful lol A recipe is innately fundamental, concise, measurable and repeatable-- exactly what science should be at the end of the day. I'll never understand why people aren't into that. I'll try this one out :laugh:

Edited by SudoNimh (04/30/22 12:13 PM)

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OfflineDemetrius18
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Re: Old LC and grain longevity viability experiment [Re: SudoNimh]
    #27759350 - 05/01/22 10:09 PM (2 years, 8 months ago)

Sorry, the grain prep is part of spitball Jedis 'the basics' something like 'how I prep my rye berries' the name of it.

Yeah the LC would be difficult to pinpoint with all the minor differences in procedure/recipe but depending on the grain

most grain soaked water that's been cooked the same (i.e. cooked grain water you pour off and save when you strain grain, then PC in a jar like the grains) I think because the endospore deal and that it's raw starches and sugars not straight dextrose or etc it's fine to cook longer than those types that can caramelize.

I'm not sure if there's stuff that's particularly prolific on rye but it seems to be one of the best overall substrate or what u call it because of the loose nature that allows gaps and  growth, also very high or good nutrition

IMO they seem to be great for most everything like gourmet stuff and take this with a boulder of salt but I think it's good for spawning hardwood loving varieties, it's very recommended for spawning most gourmet mushrooms that fruit on hardwood or logs, honestly don't know if  it will work with the kinds that need to spawn (get inocc) on hardwood like dowels or whatever

This is lack of experience with alternatives but it seems to spawn to bulk very well/successfully which I think would apply to most grains that keep their shell well and are a good size but not too big like corn, I forget how big millet is but for more fine powdery stuff it seems to cake up harder (assumption) than rye would

Also off the largest south American river getting 25 1bs for 45 dollas, not sure if it's relevant but always get told how good the taste is

But to summarize, I need to expand from my original starter and see just how valid more cost effective options are, like you said 'if it ain't broke, but I've had amazing success with it, and the price isn't rock bottom but I think it's affordable, at least compared to 3 or 5 pound bags.

So double summary I believe rye is great for a majority of types of mycelium, coming from an amateur keep in mind, look up spitballs rye Tek, and then like a paragraph or so in there's a link for 'how I prep rye' in blue.

And because they are like 'berries' they are great for spawn to bulk because they can be fairly spread and still have a lot of energy to recover and colonize,

different matter but you have to get good quality ones you know haven't been sprayed with fungicide and such, good ones will even grow if you plant them

So yeah hardier bulk action prolly the biggest highlight, you can't go wrong with 'the basics' teks for rye if you follow the sites cultivation directory, again sorry for the wierd instructions, that's just how I pull it up when checking, just don't presoak it for longer than 24-30 hours, and don't boil it (full boil) longer than like 20 minutes or it will be a starchy mess like the earlier photos, 10 minutes once fully boiling is ideal, then strained and left to steam/cool for 1.5 hrs or until not sticking to paper towel/toilet paper, then jarjar Binks.

:wink:


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InvisibleSudoNimh
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Re: Old LC and grain longevity viability experiment [Re: Demetrius18]
    #27778206 - 05/15/22 12:25 PM (2 years, 7 months ago)

Nice, thanks for the details :laugh:

I have only used Spitball's rye prep actually haha To be fair, I've only used rye once but then moved on to Bod's whole oat tek and haven't looked back. I can get 50lbs of oats/$17 locally and rye is $2/lb. I have to compete with brewers and distillers for rye :/ Not as much horse competition for oats lol why is that? /sarcasm 

Oats need only a little diatomaceous earth sprinkle and membrane-sealed tubs for ez pz long-terms. I've not really found a reason to move away from it. It was so much better than WBS for labor, better for cultivation in general and so much cheaper than rye that I've not even considered either in ages.

However... I have a few lbs of rye that I've been holding onto just in case I came across spores for whatever really benefits most from it. Then I thought recently, eh, let's just get this rye off the shelf and find whatever likes it most XD

Edited by SudoNimh (05/15/22 12:27 PM)

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OfflineDemetrius18
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Re: Old LC and grain longevity viability experiment [Re: SudoNimh]
    #27797396 - 05/29/22 08:12 AM (2 years, 7 months ago)

If oats are that much cheaper I'll have to look into it, minor sourcing issues but the difference is worth looking further.

Been looking into alternative methodology, kinda like hybrid cased cakes in bottles (jars) and such (Tupperware) and different spawn/bulk materials.

Mentioned earlier how as long as you keep the requisite sterile and nutritional procedures you can really freestyle anything, allows you to get quite versatile if one is so inclined.

https://www.shroomery.org/forums/showflat.php/Number/21168958


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OfflineDemetrius18
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Re: Old LC and grain longevity viability experiment [Re: Demetrius18]
    #27797735 - 05/29/22 01:04 PM (2 years, 7 months ago)

The culture has been salvaged fairly well, very rhizomorphic and fast going, several clones are isolate, and are going faster than the 2 other original jars of the starting 3, absolute beauty, would also like to know if anyone sees anything in that one picture, feels interpretational.

First 3 is first g2g, next 4 is another g2g a week (maybe more maybe less) after the former, and last one is a week older than 2 different strains of the same culture meeting and not growing into eachother, (myco-nlyfans(some of the best looking yet, imo))



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