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OfflineUnbornCaius
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Spores to Mycelium with Grain in 2 days * 1
    #27744658 - 04/21/22 05:09 AM (2 years, 8 months ago)

So just curious if this is a common thing or if I am just getting lucky?

I inoculated 12 WBS/gypsum/coffee quart jars.

6 with pink Buffalo and 6 with Texas ghost

I have only done one grow before years ago and it took 5 days before I noticed any growth, this time it was a solid 2 days in 8 jars so far.

I don’t see any contams yet!


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OfflineAnonuser77
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius] * 1
    #27744700 - 04/21/22 06:03 AM (2 years, 8 months ago)

Wait a few more days for them.

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InvisibleMTZ
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius] * 1
    #27744719 - 04/21/22 06:26 AM (2 years, 8 months ago)

So... Your jars are SOAKING wet. Doesn't look like much grain there either. And bacteria grows extremely fast. In about two or three days sometimes. If that's not nasties growing? You set a record of some kind. Especially for MSS to grain. Fingers crossed... 🥺🤞


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Rehears your death every morning and night. Only when you constantly live as though already a corpse will you find freedom in the martial way, and fulfill your duties without fault throughout your life. ~ The Book Of Samurai
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OfflineBobbins
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius] * 1
    #27744791 - 04/21/22 07:24 AM (2 years, 8 months ago)

:popcorn:


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OfflineA.k.aM
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Re: Spores to Mycelium with Grain in 2 days [Re: Bobbins] * 1
    #27745012 - 04/21/22 10:21 AM (2 years, 8 months ago)

Looks like myc starting, sometimes spores germ super fast especially if they’re fresh.


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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: MTZ] * 1
    #27745156 - 04/21/22 12:09 PM (2 years, 8 months ago)

Yeah they were a lot more wet than my first grow unfortunately. I used a weird tek for the WBS overnight soak and dry. And I measured my grain incorrectly so I basically had enough to fill half of each jar, then on top of inoculation the extra moisture really made it worse.

I don’t see any contamination yet, I used sterile technique and inoculated spores with self healing injection ports to lower contamination chances.

I just checked on the jars and there’s still only 8 but the growth looks thick and bright white now, there are numerous points in the one jar I took pictures of, it looks like 10 unique inoculation points started growing already

Edited by UnbornCaius (04/21/22 12:12 PM)

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Offlinehazyhorse
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius] * 1
    #27745625 - 04/21/22 05:56 PM (2 years, 8 months ago)

it looks like myc to me as well but you’re definitely not out of the woods yet. spore syringe directly to grains has a notorious contamination rate, & sometimes bacteria & trich don’t rear their head until half way through colonization. i wish you the best, but you won’t know if they are clean until you hit 100% colonization after a shake


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Invisiblefungusul
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Re: Spores to Mycelium with Grain in 2 days [Re: hazyhorse] * 1
    #27745849 - 04/21/22 09:24 PM (2 years, 8 months ago)

Welcome to Shroomery! :mushroom2:

:imo: Sorry. It has a great chance to be contaminated before 100% colonization given the amount of extra water and spore syringe directly to grains.

- Spore syringe directly to grains is a no-no because of notorious contamination rate.
- Gypsum is not required
- Coffee is not required
- Extra water increases the chances of contamination
- Good WBS teks FooMan WBS Prep or Reverse FooMan WBS Prep

Read Clean spawn checklist for the new grower to help you identify clean spawn.

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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: fungusul] * 1
    #27746169 - 04/22/22 03:35 AM (2 years, 8 months ago)

I’ll keep you guys posted.

I did the same method last time except there was less water, more grain and I had 100% success rate with 10 pint jars then. I also didn’t utilize self healing injection ports so I had to open the lids inside a SAB to inoculate with spores.

I’m hoping to at least get half of them from this, but no big deal it’s all an experience.

Edited by UnbornCaius (04/22/22 03:44 AM)

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OfflineAnonuser77
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius] * 1
    #27746183 - 04/22/22 03:58 AM (2 years, 8 months ago)

Agar isnt done for fun.

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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius] * 1
    #27746185 - 04/22/22 04:03 AM (2 years, 8 months ago)

So this is going on day 4

I understand this isn’t even close to the most efficient way to do it.

I just want to experience it for myself.

I am totally doing agar first next time and want to make clones with it as well. I just didn’t this time, no big deal.

Same jar as in the first two photos, I think this might be the farthest along but I leave the other ones alone for the most part.

This jar I leave in a visible location to kind of check on the progress.

Temps stay around 70-75 and I keep my jars in a closet, this one I keep in my SAB just in an area where I can see it.


Edited by UnbornCaius (04/22/22 04:05 AM)

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Offlinedyel
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius] * 1
    #27746238 - 04/22/22 05:02 AM (2 years, 8 months ago)

do you have pics of the syringes?I've seen spore syringes with visible mycelium wich might explain the speed

also i wonder what the guy in your avatar puts in his syringes, probably tren everyone is on fucking tren these days

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OfflineRotnpins
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius] * 1
    #27746260 - 04/22/22 05:24 AM (2 years, 8 months ago)

:popcorn:

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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: dyel] * 1
    #27749419 - 04/24/22 10:48 AM (2 years, 8 months ago)

I don’t have pics of the syringes, but they looked normal.

I got them from a reliable source. So far 12/12 jars have healthy colonization occurring and no signs of contamination. Some jars are farther than others. Once the first jar is fully colonized I will post pictures.

In every jar there are dozens of growth points it seems since I shook the jars pretty thoroughly after inoculating.

I will update at the first signs of contamination as well.

Also I have on good authority the guy in the photo was not injecting anything 😂

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Offlinedyel
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius] * 1
    #27749812 - 04/24/22 05:34 PM (2 years, 8 months ago)

My suspicion is that the syringes were already germinating and you were injecting mycelium
about your friend i suggest he switches to injectibles (if he is not doing that already) because orals will rek his liver on the long run
read about liver first pass if you are interested

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Offlinesmalltalk_canceled
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Re: Spores to Mycelium with Grain in 2 days [Re: dyel] * 1
    #27749851 - 04/24/22 06:09 PM (2 years, 8 months ago)

its all good if it goes good

we'll see who makes it out alive


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OfflineLieutenant Pan
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Re: Spores to Mycelium with Grain in 2 days [Re: smalltalk_canceled] * 1
    #27750104 - 04/25/22 12:12 AM (2 years, 8 months ago)

We wish you luck because here at the shroomery we support the growth of our members abilities.  Try to find RR's lets grow mushrooms grain prep video.  I bet $152 they're so wet they'll stall to bacteria overtime.  If your spores are that strong then re-starting new jars is probably best!


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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: dyel] * 1
    #27750270 - 04/25/22 06:07 AM (2 years, 8 months ago)

I have agar plates ordered along with parafilm and I intend to do that first on my next run. I made a bunch of mistakes this time.

I even told my girlfriend (wow this grain is way too wet and I don’t have enough to fill all these jars) before sterilization yet I didn’t continue drying them 😅

And to dyel about the orals, I feel like I know an intermediate amount about PEDs and pharmacological intervention, but thanks for the tip because that’s great advice for someone who doesn’t know how toxic orals can be on the liver.

I’m a NASM certified personal trainer and nutritionist and I’ve been studying PEDs and basic bio chemistry interactions as a hobby and interest of mine for the past 5 years so I respect when somebody speaks with caution about that stuff.

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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius] * 1
    #27750272 - 04/25/22 06:09 AM (2 years, 8 months ago)

And the syringes may have been germinating already, but I looked as closely as I could at them beforehand to look for contamination or anything weird and all I saw was clear water and spores clumped together. I shook the syringes until they were separated enough and injected them. I’m barely a novice so I don’t know for certain but I’m pretty sure I didn’t see any mycelium growth yet.

Edited by UnbornCaius (04/25/22 07:20 AM)

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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: Lieutenant Pan] * 1
    #27750320 - 04/25/22 07:19 AM (2 years, 8 months ago)

Quote:

Lieutenant Pan said:
We wish you luck because here at the shroomery we support the growth of our members abilities.  Try to find RR's lets grow mushrooms grain prep video.  I bet $152 they're so wet they'll stall to bacteria overtime.  If your spores are that strong then re-starting new jars is probably best!




I will look for that grain prep video. And right now 12/12 jars are growing mycelium. One of the jars is definitely 50% done colonizing, but there is also one jar that looks like it just started growing mycelium. This is day 7 since inoculation

To be honest it looks like somehow a lot of the water has dried up already.

If I can get the agar plates in time I might sacrifice a couple jars to see if I can get some of each strain safely transferred as backups.

Like I said by the way, when a jar either reaches full colonization or any sign of contamination I will post pics of all the jars.

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