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Offlineasteephill
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Dextrose plate?
    #27745758 - 04/21/22 07:47 PM (2 years, 8 months ago)

I have a question about plates. I was in the lab making bacterial plates and forgot to bring in malt extract to make fungus plates at the same time.. I looked around and found dextrose. I made plates with 20g/l dextrose, 5g/l yeast extract, 5g/l salt and 15g/l agar.

Will this support mycelial growth?

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OfflineSilvJailbiddi
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Re: Dextrose plate? [Re: asteephill]
    #27745794 - 04/21/22 08:26 PM (2 years, 8 months ago)

Wait, lemme get this clear, you’re making bacterial plates to support mycelium growth? Or am i high? Edit- oh I misread it. Yeah as hamloaf says, all sugar and no nutrients makes jack a dull boy

Edited by SilvJailbiddi (04/21/22 09:39 PM)

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OfflinehamloafM
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Re: Dextrose plate? [Re: SilvJailbiddi]
    #27745835 - 04/21/22 09:02 PM (2 years, 8 months ago)

That’s a lot of sugar.  You may send the mycelium into diabetic shock with that much dextrose and so little nutrient.


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Invisiblefungusul
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Re: Dextrose plate? [Re: hamloaf]
    #27745857 - 04/21/22 09:31 PM (2 years, 8 months ago)

:whathesaid:

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InvisibleSudoNimh
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Re: Dextrose plate? [Re: asteephill]
    #27746000 - 04/21/22 11:47 PM (2 years, 8 months ago)

You might have a great yeast culture with all of that sugar though! :smile:

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Offlinedyel
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Re: Dextrose plate? [Re: hamloaf]
    #27746062 - 04/22/22 12:54 AM (2 years, 8 months ago)

Quote:

hamloaf said:
That’s a lot of sugar.  You may send the mycelium into diabetic shock with that much dextrose and so little nutrient.



and high blood pressure from all that salt

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Offlineasteephill
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Re: Dextrose plate? [Re: dyel]
    #27746337 - 04/22/22 06:55 AM (2 years, 8 months ago)

K. Seems inconclusive. I'll let everyone know if it works... They grow in honey water eating dextrose. I figure there's a chance.

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OfflineDERRAYLD
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Re: Dextrose plate? [Re: asteephill]
    #27746342 - 04/22/22 06:59 AM (2 years, 8 months ago)

:lol: inconclusive?

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OfflineRotnpins
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Re: Dextrose plate? [Re: asteephill]
    #27746358 - 04/22/22 07:13 AM (2 years, 8 months ago)

:popcorn:

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Offlineasteephill
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Re: Dextrose plate? [Re: DERRAYLD]
    #27746361 - 04/22/22 07:14 AM (2 years, 8 months ago)

No science. Just smart ass replies. So, yes, inconclusive

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OfflineDERRAYLD
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Re: Dextrose plate? [Re: asteephill]
    #27746368 - 04/22/22 07:18 AM (2 years, 8 months ago)

Quote:

asteephill said:
No science. Just smart ass replies. So, yes, inconclusive




Is that really what you took from the responses?
Maybe read what people wrote and understand rather.

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Offlineasteephill
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Re: Dextrose plate? [Re: DERRAYLD]
    #27746394 - 04/22/22 07:43 AM (2 years, 8 months ago)

4% honey water typically used for liquid culture is ~3% dextrose/sugar. These plates are 2% dextrose. Yeast extract, even at the low levels added, adds more nutrition than that 4% honey. The sodium may not have been necessary, and, it may have been better to use potassium chloride, but I do not foresee any negative effects of it being there.

Now, yes, there is no science or math in any of the responses. If mushrooms got diabetes, then they would get it in honey water first AND they obviously don't have blood for blood pressure.

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Offlinedyel
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Re: Dextrose plate? [Re: asteephill]
    #27746568 - 04/22/22 10:06 AM (2 years, 8 months ago)

dude chill we were just kidding :smile:
I'm curious what bacteria are you growing on .5% salt?

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InvisibleGoatrider
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Re: Dextrose plate? [Re: dyel]
    #27746580 - 04/22/22 10:17 AM (2 years, 8 months ago)

I guess it´s meant as kind of Chapman-agar,
for checking food samples for example.
The salt hinders most bacteria, that aren´t relevant for the test.
Isolating Staphylococcus is what is done here.
I don´t think you´ll see good mycelium growth with this if any.

                :cookiemonster:


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Offlinedyel
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Re: Dextrose plate? [Re: Goatrider]
    #27746594 - 04/22/22 10:30 AM (2 years, 8 months ago)

fucking staphy, someone close to me had a very bad experience with this one

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InvisibleMTZ
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Re: Dextrose plate? [Re: dyel]
    #27746604 - 04/22/22 10:37 AM (2 years, 8 months ago)

Got it in a VA hospital after shaving. About two hours later, half my neck was a yellowish blister. Anyway, I can see how this thread is going to end up. Same every time.


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Offlinedyel
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Re: Dextrose plate? [Re: MTZ]
    #27746650 - 04/22/22 11:23 AM (2 years, 8 months ago)

It's always a hospital

I'm now curious about about op results, some fungy do tolerate high amount of sodium, we all had penicillium munching on that old parmesan we forgot about in the fridge

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Offlineasteephill
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Re: Dextrose plate? [Re: dyel]
    #27746781 - 04/22/22 01:31 PM (2 years, 8 months ago)

Almost all bacteria will grow at this concentration of salt. The most common broth recipe for bacterial growth (LB broth) contains either this % or double it.
I use it specifically for molecular cloning of DNA in e coli.

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Offlineasteephill
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Re: Dextrose plate? [Re: asteephill]
    #27748434 - 04/23/22 05:51 PM (2 years, 8 months ago)




It seems to work for now.

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InvisibleSudoNimh
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Re: Dextrose plate? [Re: asteephill]
    #27748452 - 04/23/22 06:03 PM (2 years, 8 months ago)

Wow, you beat me finishing posting this by a few minutes lol Can't wait to see more developments!

****** Original Comment ******

Jokes aside-- do you have some progress with results yet...? I truly would like to see it. Since smart-ass responses are apparently unwanted (lol), I'd like to see some RealScience(TM) in regards to your results and this concoction's efficacy.

Until then, I'm afraid that the smart-assery and general conjecture may continue. All data is good data even if any of us is wrong. It's also virtually meaningless until it's repeated and peer-reviewed.

I do believe that weapons-grade sarcasm and humor have been massive contributing factors to my scientific academia. That's also a personal sampling bias and anecdotal fallacy at once :}

Edited by SudoNimh (04/23/22 06:09 PM)

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