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OfflineUnbornCaius
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Spores to Mycelium with Grain in 2 days * 1
    #27744658 - 04/21/22 05:09 AM (2 years, 8 months ago)

So just curious if this is a common thing or if I am just getting lucky?

I inoculated 12 WBS/gypsum/coffee quart jars.

6 with pink Buffalo and 6 with Texas ghost

I have only done one grow before years ago and it took 5 days before I noticed any growth, this time it was a solid 2 days in 8 jars so far.

I don’t see any contams yet!


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OfflineAnonuser77
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius] * 1
    #27744700 - 04/21/22 06:03 AM (2 years, 8 months ago)

Wait a few more days for them.

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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius] * 1
    #27744719 - 04/21/22 06:26 AM (2 years, 8 months ago)

So... Your jars are SOAKING wet. Doesn't look like much grain there either. And bacteria grows extremely fast. In about two or three days sometimes. If that's not nasties growing? You set a record of some kind. Especially for MSS to grain. Fingers crossed... 🥺🤞


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Rehears your death every morning and night. Only when you constantly live as though already a corpse will you find freedom in the martial way, and fulfill your duties without fault throughout your life. ~ The Book Of Samurai
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OfflineBobbins
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius] * 1
    #27744791 - 04/21/22 07:24 AM (2 years, 8 months ago)

:popcorn:


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OfflineA.k.aM
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Re: Spores to Mycelium with Grain in 2 days [Re: Bobbins] * 1
    #27745012 - 04/21/22 10:21 AM (2 years, 8 months ago)

Looks like myc starting, sometimes spores germ super fast especially if they’re fresh.


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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: MTZ] * 1
    #27745156 - 04/21/22 12:09 PM (2 years, 8 months ago)

Yeah they were a lot more wet than my first grow unfortunately. I used a weird tek for the WBS overnight soak and dry. And I measured my grain incorrectly so I basically had enough to fill half of each jar, then on top of inoculation the extra moisture really made it worse.

I don’t see any contamination yet, I used sterile technique and inoculated spores with self healing injection ports to lower contamination chances.

I just checked on the jars and there’s still only 8 but the growth looks thick and bright white now, there are numerous points in the one jar I took pictures of, it looks like 10 unique inoculation points started growing already

Edited by UnbornCaius (04/21/22 12:12 PM)

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Offlinehazyhorse
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius] * 1
    #27745625 - 04/21/22 05:56 PM (2 years, 8 months ago)

it looks like myc to me as well but you’re definitely not out of the woods yet. spore syringe directly to grains has a notorious contamination rate, & sometimes bacteria & trich don’t rear their head until half way through colonization. i wish you the best, but you won’t know if they are clean until you hit 100% colonization after a shake


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Re: Spores to Mycelium with Grain in 2 days [Re: hazyhorse] * 1
    #27745849 - 04/21/22 09:24 PM (2 years, 8 months ago)

Welcome to Shroomery! :mushroom2:

:imo: Sorry. It has a great chance to be contaminated before 100% colonization given the amount of extra water and spore syringe directly to grains.

- Spore syringe directly to grains is a no-no because of notorious contamination rate.
- Gypsum is not required
- Coffee is not required
- Extra water increases the chances of contamination
- Good WBS teks FooMan WBS Prep or Reverse FooMan WBS Prep

Read Clean spawn checklist for the new grower to help you identify clean spawn.

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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: fungusul] * 1
    #27746169 - 04/22/22 03:35 AM (2 years, 8 months ago)

I’ll keep you guys posted.

I did the same method last time except there was less water, more grain and I had 100% success rate with 10 pint jars then. I also didn’t utilize self healing injection ports so I had to open the lids inside a SAB to inoculate with spores.

I’m hoping to at least get half of them from this, but no big deal it’s all an experience.

Edited by UnbornCaius (04/22/22 03:44 AM)

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OfflineAnonuser77
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius] * 1
    #27746183 - 04/22/22 03:58 AM (2 years, 8 months ago)

Agar isnt done for fun.

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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius] * 1
    #27746185 - 04/22/22 04:03 AM (2 years, 8 months ago)

So this is going on day 4

I understand this isn’t even close to the most efficient way to do it.

I just want to experience it for myself.

I am totally doing agar first next time and want to make clones with it as well. I just didn’t this time, no big deal.

Same jar as in the first two photos, I think this might be the farthest along but I leave the other ones alone for the most part.

This jar I leave in a visible location to kind of check on the progress.

Temps stay around 70-75 and I keep my jars in a closet, this one I keep in my SAB just in an area where I can see it.


Edited by UnbornCaius (04/22/22 04:05 AM)

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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius] * 1
    #27746238 - 04/22/22 05:02 AM (2 years, 8 months ago)

do you have pics of the syringes?I've seen spore syringes with visible mycelium wich might explain the speed

also i wonder what the guy in your avatar puts in his syringes, probably tren everyone is on fucking tren these days

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OfflineRotnpins
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius] * 1
    #27746260 - 04/22/22 05:24 AM (2 years, 8 months ago)

:popcorn:

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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: dyel] * 1
    #27749419 - 04/24/22 10:48 AM (2 years, 8 months ago)

I don’t have pics of the syringes, but they looked normal.

I got them from a reliable source. So far 12/12 jars have healthy colonization occurring and no signs of contamination. Some jars are farther than others. Once the first jar is fully colonized I will post pictures.

In every jar there are dozens of growth points it seems since I shook the jars pretty thoroughly after inoculating.

I will update at the first signs of contamination as well.

Also I have on good authority the guy in the photo was not injecting anything 😂

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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius] * 1
    #27749812 - 04/24/22 05:34 PM (2 years, 8 months ago)

My suspicion is that the syringes were already germinating and you were injecting mycelium
about your friend i suggest he switches to injectibles (if he is not doing that already) because orals will rek his liver on the long run
read about liver first pass if you are interested

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Re: Spores to Mycelium with Grain in 2 days [Re: dyel] * 1
    #27749851 - 04/24/22 06:09 PM (2 years, 8 months ago)

its all good if it goes good

we'll see who makes it out alive


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OfflineLieutenant Pan
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Re: Spores to Mycelium with Grain in 2 days [Re: smalltalk_canceled] * 1
    #27750104 - 04/25/22 12:12 AM (2 years, 8 months ago)

We wish you luck because here at the shroomery we support the growth of our members abilities.  Try to find RR's lets grow mushrooms grain prep video.  I bet $152 they're so wet they'll stall to bacteria overtime.  If your spores are that strong then re-starting new jars is probably best!


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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: dyel] * 1
    #27750270 - 04/25/22 06:07 AM (2 years, 8 months ago)

I have agar plates ordered along with parafilm and I intend to do that first on my next run. I made a bunch of mistakes this time.

I even told my girlfriend (wow this grain is way too wet and I don’t have enough to fill all these jars) before sterilization yet I didn’t continue drying them 😅

And to dyel about the orals, I feel like I know an intermediate amount about PEDs and pharmacological intervention, but thanks for the tip because that’s great advice for someone who doesn’t know how toxic orals can be on the liver.

I’m a NASM certified personal trainer and nutritionist and I’ve been studying PEDs and basic bio chemistry interactions as a hobby and interest of mine for the past 5 years so I respect when somebody speaks with caution about that stuff.

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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius] * 1
    #27750272 - 04/25/22 06:09 AM (2 years, 8 months ago)

And the syringes may have been germinating already, but I looked as closely as I could at them beforehand to look for contamination or anything weird and all I saw was clear water and spores clumped together. I shook the syringes until they were separated enough and injected them. I’m barely a novice so I don’t know for certain but I’m pretty sure I didn’t see any mycelium growth yet.

Edited by UnbornCaius (04/25/22 07:20 AM)

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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: Lieutenant Pan] * 1
    #27750320 - 04/25/22 07:19 AM (2 years, 8 months ago)

Quote:

Lieutenant Pan said:
We wish you luck because here at the shroomery we support the growth of our members abilities.  Try to find RR's lets grow mushrooms grain prep video.  I bet $152 they're so wet they'll stall to bacteria overtime.  If your spores are that strong then re-starting new jars is probably best!




I will look for that grain prep video. And right now 12/12 jars are growing mycelium. One of the jars is definitely 50% done colonizing, but there is also one jar that looks like it just started growing mycelium. This is day 7 since inoculation

To be honest it looks like somehow a lot of the water has dried up already.

If I can get the agar plates in time I might sacrifice a couple jars to see if I can get some of each strain safely transferred as backups.

Like I said by the way, when a jar either reaches full colonization or any sign of contamination I will post pics of all the jars.

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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27750336 - 04/25/22 07:43 AM (2 years, 8 months ago)

The problem with wet grains, is that sometimes the wet colonized grain jars will be so bacterial that they moment you spawn them, the trich shows up long before a first flush can compete.

Ive lost 200 subs to that shit when I was starting out, easily.
Now I dont even bother knocking up jars like that.

The people warning against wet grain are trying to help you avoid loss and pain


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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: smalltalk_canceled]
    #27750424 - 04/25/22 09:22 AM (2 years, 8 months ago)

You’re probably right, and I’m not arguing against that at all. I would rather experience it for myself than to just go by the word of a stranger, even if everyone here knows more than me and has years of experience.

I could just follow what everyone says word for word, but if I did that I wouldn’t have experienced the failure on my own and I would be able to place blame. If I fail here I know it 100% was my fault.

I do appreciate all the advice here and I am listening, I just want to follow through with this to see exactly how contamination comes to happen, if it does, even though my goal is to find success.

After all, the master has made more mistakes than the novice has even tried.

In this stage of my journey I think I would learn more from making mistakes and following through than to just scrap what I have now, so that I can experience the mistake on my own and gain insight and experience from that in itself for next time.

I think the data I can acquire from every run at this stage is more important to me than avoiding mistakes, but that’s just me.

I have plenty of time and I’m not in any rush.

That being said I still accept criticism here and advice and I will look into that and use that for next time.

I already started documenting this run in a notebook and took pictures (I know I said I didn’t have pictures of the spore syringes, I technically do but the photo only shows the label and you can’t see the liquid or spores behind it)

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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: smalltalk_canceled]
    #27750429 - 04/25/22 09:28 AM (2 years, 8 months ago)



This is the jar that is farthest along I think

By the way I just wiped the jar off with a Lysol wipe to clean my fingerprints off because they were obstructing the view with the flash on. So that’s why the outside my appear smudged or wet

Edited by UnbornCaius (04/25/22 09:31 AM)

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OfflineDERRAYLD
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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27750431 - 04/25/22 09:29 AM (2 years, 8 months ago)

As much as it's good to learn from your mistakes it can also be incredibly demotivating, don't get too hung up on the lesson if it's going to cause you to lose interest in the hobby.

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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: DERRAYLD]
    #27750439 - 04/25/22 09:39 AM (2 years, 8 months ago)

Anyway, I’ll just share my progress here and leave updates in case anyone is interested.

I appreciate the care everyone has for me maintaining an interest in this, and believe me mycology has been an interest of mine for about 5 years and I own and have read dozens of mycology and mushroom identification books, I’m on mushroom facebook groups and frequently go foraging for mushrooms in my area.

I strongly believe mushrooms can change and even save the world as Paul Stamets says.

I just haven’t had a lot of experience with cultivation because I’ve been so busy, but now I have time for it so I’m taking advantage of it.

I’m not worried about a failed grow crushing my spirits, I understand that’s just how things go if you make mistakes and I won’t take it too personally.

I think maybe the reason the spores germinated so quickly was maybe due to the source. I’ve been looking into it and the source seems to be highly appraised

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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27750521 - 04/25/22 10:58 AM (2 years, 8 months ago)

Quote:

UnbornCaius said:
And the syringes may have been germinating already, but I looked as closely as I could at them beforehand to look for contamination or anything weird and all I saw was clear water and spores clumped together. I shook the syringes until they were separated enough and injected them. I’m barely a novice so I don’t know for certain but I’m pretty sure I didn’t see any mycelium growth yet.



you just might have hit jackpot with some Ronnie Coleman genetics and got hyper responders there :lol:
lightweight babe lightweight

good luck on your grow and keep us posted

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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: dyel]
    #27758776 - 05/01/22 02:06 PM (2 years, 8 months ago)

Thanks Dyel :grin: I think you are right, the Texas ghost genetics seem to be extremely aggressive. The source I got it from seems to be highly reputable and passionate about mycology

It seems 12/12 jars are healthy and free of contamination as far as I can tell. The Texas ghost strain has grown a lot more aggressively than the pink Buffalo. Once the jars reached 30% colonization I beat them with a tennis ball to break them up and then allowed them to fully colonize which happened within a day or two after that.

The first jar to reach 100% was a Texas ghost jar which took 9 days total from spore. Right now there are two pink Buffalo jars that have not fully colonized yet but should be there within a couple of days I would imagine.

I used a local field aged straw and grass fed horse manure that I collected myself which were in nugget form, they were leeched and dried naturally on the pasture.

I mixed about 20% vermiculite and 5% gypsum with 9 quarts of crumbled manure, brought to field capacity, put the substrate into a black lab garbage bag, and then used a steam pasteurization chamber with the temp gauge stuck directly in the center of the bag which was suspended using a wire rack to keep it out of the collecting water.

I let the temp get to 150-160F and kept it there for an hour.

Once that was done I let it cool to about 80F and set up my mono tub, it has 6 total holes which are 2” diameter. The lower holes are 3” above the bottom of the tub.

I used a black lawn bag and set a liner after thoroughly sanitizing the inside of the tub, and put a layer of manure sub, then grain spawn. I continued this until I used about 4 quarts of grain.

Before I inoculated with grain spawn I took a moment to get a good sense of what the spawn smelled like, it was incredibly earthy and smelled like textbook mushroom smell. Really pleasant earthy smell in each of the jars, nothing smelled weird or off. The manure smelled like fresh soil on a cool summer night after a brief rain shower.

Now I will wait for the pink buffalo to finish colonizing their grain and wait for the fruiting chamber to colonize as well.

I’m hoping to get some good samples to agar plates from the fruits.

Here are a bunch of progress pictures since my last post.


Edited by UnbornCaius (05/02/22 12:00 AM)

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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27759577 - 05/02/22 06:08 AM (2 years, 8 months ago)

I'm curious how fast will these be as LC or LI
ofc it is still on trial for yeald and potency before being named a winner
:popcorn:

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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: dyel]
    #27760004 - 05/02/22 01:26 PM (2 years, 8 months ago)

I’m really curious as well.

I will keep the thread updated, I will probably take photos and make notes as I go like my last post and then make a longer post all at once with photos and chronology.

So it hasn’t even been 24 hours since I inoculated the bulk substrate with the grain and I already see mycelium growth coming through to the top of the manure layer.

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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27760542 - 05/02/22 07:59 PM (2 years, 8 months ago)

Looking great I love an underdog story !

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Re: Spores to Mycelium with Grain in 2 days [Re: 1Lowkey75]
    #27760823 - 05/03/22 12:35 AM (2 years, 8 months ago)

Omg haha I love the heavy amount of doubters at the beginning of this thread spewing out notes from their rule books, telling you how death was imminent, your pep talks to yourself, and now I’m looking at many jars of colonized grain and a set monotub. You also are doing all of this from scratch with all of your own materials making your own bulk substrate. I hope you have a bountiful supply of mushrooms soon!

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Re: Spores to Mycelium with Grain in 2 days [Re: PGwojowski] * 1
    #27760853 - 05/03/22 01:43 AM (2 years, 8 months ago)

Quote:

PGwojowski said:
Omg haha I love the heavy amount of doubters at the beginning of this thread spewing out notes from their rule books, telling you how death was imminent, your pep talks to yourself, and now I’m looking at many jars of colonized grain and a set monotub. You also are doing all of this from scratch with all of your own materials making your own bulk substrate. I hope you have a bountiful supply of mushrooms soon!




There's a healthy amount of doubters for a reason, those jars are all bacterial.
That doesn't mean he won't get fruit but it also means he may get nothing.

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Re: Spores to Mycelium with Grain in 2 days [Re: PGwojowski]
    #27760870 - 05/03/22 03:06 AM (2 years, 8 months ago)

I hope op gets to harvest but a few unwanted spores in the syringe and he could be raising a mold farm and believe me you don't want to be the one cleaning that shit.
what is recommended here is to have control over your grows (as much as possible with limited resources) and not following the spray and pray mentality
there is another place for that and it's called reddit i believe

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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: dyel]
    #27761753 - 05/03/22 06:08 PM (2 years, 8 months ago)

I agree using the spore to grain method is not desirable and I didn’t really think about it when I was doing it, since I did it the first time I grew and had 100% success.

Now I know and certainly will do spore to agar beforehand to make sure, but let’s consider this thread my personal experiment.

Even if I have success I’ll understand it was luck, so I appreciate all the quality advice and everything.

I learned that spore to grain isn’t optimal because collecting spores is an inherently risky operation in terms of contamination, unless your entire grow room and spore print area are in the same room that has positive pressure, double filtered through a HEPA system, and there is a decontamination chamber before entry… and even then there is still risk of contamination especially if the substrate isn’t 100% sterilized through a double ended autoclave that goes through a wall into your grow room.

Basically none of that crossed my mind how the spore syringes were made, so now I know.. but that’s alright because making mistakes is all part of the learning experience and that’s what we have communities like this for, to share our experience and help eachother.

Edited by UnbornCaius (05/03/22 06:09 PM)

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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27761778 - 05/03/22 06:31 PM (2 years, 8 months ago)

Exactly. Even if this works, later syringes may not be clean enough.
The proposition to go agar is about reliability, if one wants to throw the dice and be reliant on other people's work, spore solutions to grain is perfectly acceptable.


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OfflineUnbornCaius
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Re: Spores to Mycelium with Grain in 2 days [Re: smalltalk_canceled]
    #27767488 - 05/07/22 03:09 PM (2 years, 8 months ago)

Here is a small update,

I realized I had about a cc of spores left in each syringe, and I had a jar of grain left from both strains. I bought a 12 pack of 4oz jam jars that I used to make malt extract agar jars.

1.5 days ago I put ghost spores in 3 jars, pink Buffalo spores in 3 jars, I put a little piece of mycelium from ghost grains in 3 jars, and mycelium from pink Buffalo in 3 jars.

So far all of the grain to agar transfers have started growing onto the agar, no contamination visible.

None of the spore to agar jars have grown anything, I don’t see contam yet either.

The tubs seem to be growing really fast according to what I’ve seen in most grows.

The furthest colonized tub is about 70 quarts day 6 after spawn to bulk substrate and it is the ghost strain, a lot of rhizomorphic growth

The other tub is about 92 quarts day 4 after spawn to bulk, and it is the pink Buffalo strain, a lot of fluffy tomentose growth in comparison to the ghost tub

So I’ve been thinking about casing teks, I just read this book called “Conscious Cultivator: Psilocybe Edition” and there is something called a cheesecloth tek which seems really interesting. I looked it up and didn’t see really anything here on shroomery but I found some pretty unscientific ways of testing it other places.

I think this time I’m going to just do 50% peat moss 50% vermiculite, increase ph with lime to about 7.2-7.8 and then stabilize the ph with gypsum, but I do want to run tests on the cheesecloth casing when I get some clones and have fine tuned control of all the variables. That way I can make sure every variable except the casing is different, and I would run the experiment multiple times and track the data to compare the differences.

Anyway if anyone has any advice on a good casing tek I would be happy to hear it because this is my first time growing in a bulk substrate.

EDIT: I’ve decided to just run these mono tubs without a casing layer, since I don’t think I really need it, but I will be doing experiments on it later on in future grows.

By the way I will get pics of the agar jars when they look more interesting.

The photos of the tubs are kind of unclear because they’re taken behind the plastic. I don’t want to open the lids until they are ready for a casing layer, I don’t want to unnecessarily introduce contaminants when it isn’t 100% colonized, and I don’t want to release CO2 and humidity which I think may give mixed signals to the mycelium to start pinning. I’d rather make effective and efficient steps rather than poking my nose in the tub everyday, maybe it’s not that big of a deal but that’s just my noob opinion




Edited by UnbornCaius (05/07/22 09:10 PM)

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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27767938 - 05/07/22 09:50 PM (2 years, 8 months ago)

Here are some of the grain to agar jars, and I decided to start fruiting the ghost tub since the mycelium looked about 80%-100% done colonizing


Edited by UnbornCaius (05/07/22 10:22 PM)

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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27769818 - 05/09/22 01:39 PM (2 years, 8 months ago)

Day 8: Update of the tubs

Pink Buffalo still has tape over the holes until the sub colonized.
Still haven’t opened the lids, there is still a lot of light moisture in the tubs.


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Re: Spores to Mycelium with Grain in 2 days [Re: fungusul]
    #27769848 - 05/09/22 02:02 PM (2 years, 8 months ago)

Quote:

fungusul said:
Welcome to Shroomery! :mushroom2:

:imo: Sorry. It has a great chance to be contaminated before 100% colonization given the amount of extra water and spore syringe directly to grains.

- Spore syringe directly to grains is a no-no because of notorious contamination rate.
- Gypsum is not required
- Coffee is not required
- Extra water increases the chances of contamination
- Good WBS teks FooMan WBS Prep or Reverse FooMan WBS Prep

Read Clean spawn checklist for the new grower to help you identify clean spawn.



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Re: Spores to Mycelium with Grain in 2 days [Re: Ashtray161]
    #27770050 - 05/09/22 04:33 PM (2 years, 8 months ago)

I do have 6 seemingly healthy cultures from my grain spawn to agar with no visible contamination, and 6 MSS to agar that just started germinating. (I see some suspicious areas on the MSS-agar jars but not certain yet, I moved them in a separate area in isolation) I guess we will see what happens here.

I hope I can get fruits here, and I don’t see anything indicative of contamination yet but if it does pop up oh well not the end of the world.

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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27770598 - 05/10/22 01:13 AM (2 years, 8 months ago)

i think you’ll get fruits man. you can get away with less than stellar stuff & get fruits which is nice. the hard part is getting it to perform super well. all just about dialing things in


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Re: Spores to Mycelium with Grain in 2 days [Re: hazyhorse]
    #27770813 - 05/10/22 07:45 AM (2 years, 8 months ago)

Yeah you’ll almost definitely get a decent yield off those.


The focus on shroomery is mainly on doing things in a clean and reliable method, but if you just want some fucking mushrooms already then syringes are fine. Quick and (possibly) dirty methods are effective if youre not doing this as a serious hobby.


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Re: Spores to Mycelium with Grain in 2 days [Re: A.k.a]
    #27771258 - 05/10/22 01:59 PM (2 years, 8 months ago)

Well I’ve got 12 jars of good looking agar cultures now so if my tubs don’t do well I think I’ll be okay I can just try again.

And thanks if I can just get a couple to clone with I’ll be happy.

I think my technique has been pretty good, besides the mistake of wet grain. My main mistake was doing spore to grain but I really do think the syringe was pretty clean because my spore to agar jars don’t seem to have any contamination yet but they’ve already germinated and started growing.

I’m pretty skeptical on those though and I have them put aside in case they do contaminate.

Anyway can’t wait for the next run I already got 12 more quarts of grain spawn PCd and ready to go as of this morning.

I really want to see if I can get a haploid from the ghost and pink Buffalo and try to breed the two, that would be pretty cool

But yeah once I get everything dialed in and fine tuned I think I want to run some very controlled experiments and document them all

Edited by UnbornCaius (05/10/22 02:38 PM)

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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27771294 - 05/10/22 02:24 PM (2 years, 8 months ago)

Quote:

UnbornCaius said:








I love to see people experimenting with different casing layers, I may try this on one of my tubs.

Your methods an success are intriguing. Don't worry about some comments here. Most people have a " ONLY USE WHAT WORKS " attitude.

If you are using an method that hasn't be seen or hasn't been perfected keep documenting it.

Just me personally I would never ever go straight to grains, no matter how clean or reliable my spores are. Your Myc growth looks good. Keep this updated.


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Re: Spores to Mycelium with Grain in 2 days [Re: BiggDaddyCane]
    #27771308 - 05/10/22 02:36 PM (2 years, 8 months ago)

Thanks Bigg, lesson learned about spore to grain, and I will continue to document everything I do. I am having a lot of ideas already about different experiments I could run, and I’ve been writing them down in my log journal.

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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27771372 - 05/10/22 03:50 PM (2 years, 8 months ago)

Spore to grain was the main method used for a long time.

When I started I probably ran 30 jars from syringe with no issues, then when I started agar lost like 1/3 :lol:


Gotta have the sterile technique down.


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Re: Spores to Mycelium with Grain in 2 days [Re: A.k.a]
    #27771383 - 05/10/22 03:57 PM (2 years, 8 months ago)

It really shows how much of a miracle it is that these things thrive in the wild when competing with the endless waves of bacteria, molds, yeast, animals, etc…

Life is insanely vast and impressive

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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27772098 - 05/11/22 12:14 AM (2 years, 8 months ago)

So fingers still crossed that there’s no contamination.

I kind of wanted to give it a little more air since after I put the filters in I haven’t needed to mist, so I took the lids off and fanned them. While I did that I took a couple photos.

There is a photo here of the PB tub with tape on the holes, but I just put filters on it since it started a couple days later than the ghost tub

I checked for a smell but couldn’t smell much of anything at all besides a faint mushroom/substrate/rain smell


Edited by UnbornCaius (05/11/22 12:38 AM)

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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27772486 - 05/11/22 10:31 AM (2 years, 8 months ago)

you don’t need to fan at all. it doesn’t help anything & your air should be exchanged passively & consistently through dialing in your tubs. both tubs look dry but that first tub looks super dry to me but it could be the pic. you want your surface to look like this at practically all times


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Re: Spores to Mycelium with Grain in 2 days [Re: hazyhorse]
    #27772723 - 05/11/22 02:19 PM (2 years, 8 months ago)

Thanks hazy, yeah I never fanned it or even opened the lid until then so I was surprised it was that dry. I’ve been misting a couple times a day now and just letting it evaporate on its own

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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27772901 - 05/11/22 04:53 PM (2 years, 8 months ago)

no problem! yeah, dialing in your tubs can be tricky at first. i find it’s good to check in on stuff while it’s colonizing until you get a feel for how your tubs perform in your specific climate. ideally you shouldn’t have to mist until the first flush starts to come in, but if you haven’t run too many tubs you might not know how much to open the holes. polyfill & giant holes covered in micropore tape can be tricky to dial in. i’ve had really good success with  pasty’s ez dialed monos & just some micropore tape over the lower/upper holes. if they seem to want more FAE as the flush comes in, i’ll take off the tape. lately i’ve been able to just let it ride with no interference on my part

just FYI if your subs get too dry, they stop holding moisture on the surface in beads, so if you’re misting a lot but don’t notice the tiny beads after the mist, that may be why.


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Re: Spores to Mycelium with Grain in 2 days [Re: hazyhorse]
    #27773014 - 05/11/22 06:52 PM (2 years, 8 months ago)

Thanks hazy I’ll check it out!

Since I’ve misted last night I checked on them today and it looks like there is still a consistent layer of mist beads sitting on top of the mycelium lawn.

So the advice is to just maintain that level of moisture on the surface and just let the FAE evaporate the moisture on its own?

And yeah my filters can definitely be improved and dialed in quite a lot more than they are. I used two different styles of filters for the tubs. Lots of learning to do

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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27773040 - 05/11/22 07:14 PM (2 years, 8 months ago)

Quote:

UnbornCaius said:
Since I’ve misted last night I checked on them today and it looks like there is still a consistent layer of mist beads sitting on top of the mycelium lawn.

So the advice is to just maintain that level of moisture on the surface and just let the FAE evaporate the moisture on its own?




yup!! that’s pretty much exactly what you wanna do (:

it’s always a learning process & as long as you’re paying attention you’ll get better with every grow. eventually you get things figured out to the point where it’s mostly dialed in. everyone’s conditions are different so there’s almost always a bit of work to be done before you can really make stuff set & forget


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Re: Spores to Mycelium with Grain in 2 days [Re: hazyhorse]
    #27773131 - 05/11/22 08:05 PM (2 years, 8 months ago)

Cool thank you! I’m looking forward to getting to the point where I can start reliably experimenting and getting meaningful data

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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27773140 - 05/11/22 08:08 PM (2 years, 8 months ago)

just have fun with it! you’ll end up with more mushrooms than you’ll ever need way faster than you realize lol


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Re: Spores to Mycelium with Grain in 2 days [Re: hazyhorse]
    #27773240 - 05/11/22 09:35 PM (2 years, 8 months ago)

Good haha exactly what I’m looking for

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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27775080 - 05/13/22 04:33 AM (2 years, 8 months ago)

Here’s an update

Just misted


Ghost tub on the left, pink Buffalo on the right



Also miraculously my 6 grain to agar jars are totally contamination free as far as I can tell

My 6 spore to agar jars also seem to be totally contamination free, the only thing I see is pretty healthy rhizomorphic mycelium

Also it must have been a fluke that the ghost colonized faster, because the pink buffalo agar jars totally reached 100% before the ghost ones and the spores also took off faster

Edited by UnbornCaius (05/13/22 04:33 AM)

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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27779821 - 05/16/22 04:57 AM (2 years, 7 months ago)

Update on ghost tub

First pins showing

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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27787744 - 05/22/22 03:39 AM (2 years, 7 months ago)

So the “ghost” tub doesn’t seem to be ghost whatsoever.

The pink Buffalo tub just started pinning today

And I think there are FAE issues because some are growing sideways.


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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27787745 - 05/22/22 03:41 AM (2 years, 7 months ago)

The picture isn’t from today but a couple days ago, and about half of them have been harvested. Not a lot of yield on the first flush I would say there is almost an ounce dried so far

Edited by UnbornCaius (05/22/22 07:06 AM)

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Re: Spores to Mycelium with Grain in 2 days [Re: UnbornCaius]
    #27789016 - 05/23/22 04:06 AM (2 years, 7 months ago)

MS luck of the draw, maybe try cloning one of the better clusters to try and get something more consistent

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Re: Spores to Mycelium with Grain in 2 days [Re: dyel]
    #27789315 - 05/23/22 10:39 AM (2 years, 7 months ago)

I have 24 agar jars fully colonized, 12 are from grains of each strain and 12 are from MSS each strain.

I plan on taking clones from the second flush of the “ghost” which I assume is probably B+ and not ghost. And also from the pink Buffalo

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