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OfflineFunfarm
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Re: Weird color [Re: WyoMX]
    #27743379 - 04/20/22 10:02 AM (2 years, 8 months ago)

Maybe I'm getting bad Agar plates thru order? Can anyone suggest a good place for agar plates? I checked the trusted vendors but didnt see any?

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OfflineFunfarm
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Re: Weird color [Re: Funfarm]
    #27743433 - 04/20/22 10:39 AM (2 years, 8 months ago)

420!
Looking for good agar plates to order

Edited by Funfarm (04/20/22 10:40 AM)

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OfflineFunfarm
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Re: Weird color [Re: Funfarm]
    #27744086 - 04/20/22 06:17 PM (2 years, 8 months ago)

Grandma is laughing at me, she says they look like urinal cakes. The mold is growing right where the spores are.


Edited by Funfarm (04/20/22 06:19 PM)

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Invisible0001001
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Re: Weird color [Re: Funfarm] * 1
    #27744218 - 04/20/22 07:32 PM (2 years, 8 months ago)

Just make your own agar plates?!?!?!??!?!?

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OfflineFunfarm
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Re: Weird color [Re: 0001001]
    #27745240 - 04/21/22 01:30 PM (2 years, 8 months ago)

I did see a pretty good receipe an process on line involving using a glass liquor bottle. Looked like all the Ingredients could be obtained. Honestly it was my fault not the plates

Edited by Funfarm (04/21/22 02:35 PM)

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OfflineFunfarm
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Re: Weird color [Re: Funfarm]
    #27754754 - 04/28/22 09:05 AM (2 years, 8 months ago)

Hello I was wondering how long it takes your agar plates 2 become fully colonised with mycelium once inoculated?

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OfflineB Traven
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Re: Weird color [Re: Funfarm]
    #27754826 - 04/28/22 09:46 AM (2 years, 8 months ago)

Quote:

Funfarm said:
Hello I was wondering how long it takes your agar plates 2 become fully colonised with mycelium once inoculated?




It depends on a lot of different factors, but full colonization is rarely your goal anyway.

I'd say you should have spore germination by about the 2 week mark. Then you should really be off to the races- immediate transfer of germinated patches, and then sequential transfers about once a week.

Failure rates can be high on agar, depending on your plate prep, spore solution and sterile techniques. But the great advantage is that you can easily start lots of plates until you find some winners.

That mold you have might have come straight from the spore syringe. Or, the liquid you  squirted encouraged its growth. Keep trying, you'll get it!

I have found that my failure rate dropped once I started streaking spore prints onto plates, rather than using syringes. You could give that a try if you get frustrated.


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OfflineFunfarm
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Re: Weird color [Re: B Traven]
    #27754966 - 04/28/22 11:26 AM (2 years, 8 months ago)

Hey thanks for the great response I appreciate it

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OfflineFunfarm
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Re: Weird color [Re: Funfarm]
    #27755737 - 04/28/22 09:06 PM (2 years, 8 months ago)

So heres the next line of question.... what causes an agar plate to stall in growth? Has the plate run out of sugar fuel, all being absorbed to promote what was rapid colonization only to stall in mycellium, is the agar plate running out of fuel? Perhaps remove the healthy thick percentage of thick mycellium to a new fresh agar plate with fresh fuel to further substantiate new aggressive colonization? Is there validity to this new ponder? Hmmm? Rapid growth, no more growth.... biopsy old plate to new fresh pour plate, does the mycellium then continue growth pattern? Inquiring mind wants to know, to much vodka and sativa dom psychoactive thc has mindless fool wondering. Glorious triumphant, happy growth and trips for you.

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OfflineB Traven
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Re: Weird color [Re: Funfarm]
    #27756161 - 04/29/22 05:55 AM (2 years, 8 months ago)

Mycelium will typically colonize all of a plate if it has the chance to, and then often start pinning. Or, if left sitting long enough, it'll dry out. Plates that I forget about, if they're not contaminated, tend to either dry out or kick out little mini fruits that dump spores all over them.

Colonizing all of the available substrate, and then using up most of the available nutrients, is a major trigger for fruiting, because that's when it's time to kick out spores to colonize new locations.

If you get a plate that stalls out before fully colonizing, then it's likely an issue with contamination.

But again, you're usually not trying for full colonization. You're trying to get transfers before the mycelium is hopelessly intertwined with any potential contaminants. Only on plates that you were using for inoculation, or plates you wanted to grow pins on, would you want full colonization.

When you start collecting and obsessing over plates, that's when the real geek-out begins 👍

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OfflineFunfarm
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Re: Weird color [Re: B Traven]
    #27756313 - 04/29/22 09:09 AM (2 years, 8 months ago)

So the name of the game is constant agar 2 agar transfer of healthy mycellium chunks?

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InvisibleAshtray161
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Re: Weird color [Re: Funfarm]
    #27756315 - 04/29/22 09:12 AM (2 years, 8 months ago)

Quote:

Funfarm said:
So the name of the game is constant agar 2 agar transfer of healthy mycellium chunks?




That's indeed the name of the game good sir, madam, or human.


--------------------
(You Know What Time It Is)
Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086:elmo:
"You never have to prove the fool a fool, just let them speak."
Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED.
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OfflinehaniblecterS
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Re: Weird color [Re: WyoMX]
    #27757282 - 04/30/22 07:32 AM (2 years, 8 months ago)

gotta disagree. messing with a whole 'nother system to avoid some jar contams makes no sense. truly believe it does new people a diservice telling them agar is the only way when MS syringe will get it done.

but ya, SAB is the only way. hole saws work but you have to be super slow, almost to the point of melting the plastic.

side note, cut the top out and duct tape some plexiglass to it. itll help SOO MUCH with visibility.


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InvisibleAshtray161
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Re: Weird color [Re: haniblecter]
    #27757493 - 04/30/22 11:29 AM (2 years, 8 months ago)

Quote:

haniblecter said:
gotta disagree. messing with a whole 'nother system to avoid some jar contams makes no sense. truly believe it does new people a diservice telling them agar is the only way when MS syringe will get it done.

but ya, SAB is the only way. hole saws work but you have to be super slow, almost to the point of melting the plastic.

side note, cut the top out and duct tape some plexiglass to it. itll help SOO MUCH with visibility.




You're dead wrong man. Agar is needed in this hobby and the quicker you learn it the better. Agar is the only reliable way to go with MS syringes.

OP, learn agar.


--------------------
(You Know What Time It Is)
Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086:elmo:
"You never have to prove the fool a fool, just let them speak."
Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED.
Be Gay, Do Crime 161 1312

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OfflineB Traven
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Re: Weird color [Re: haniblecter]
    #27757569 - 04/30/22 12:41 PM (2 years, 8 months ago)

Quote:

haniblecter said:
gotta disagree. messing with a whole 'nother system to avoid some jar contams makes no sense. truly believe it does new people a diservice telling them agar is the only way when MS syringe will get it done.






Agar isn't the only way by any means- PF tek usually works fine with straight spore syringes, if you want to keep it simple.

But I really wish I had been more strongly discouraged from injecting spores into grain jars. It wasted a lot of my time, and taught me very little.


--------------------
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InvisibleAshtray161
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Re: Weird color [Re: B Traven]
    #27757585 - 04/30/22 01:09 PM (2 years, 8 months ago)

That's true, I didnt think about PF tek. That's the only scenario I can think of where agar isnt needed


--------------------
(You Know What Time It Is)
Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086:elmo:
"You never have to prove the fool a fool, just let them speak."
Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED.
Be Gay, Do Crime 161 1312

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OfflineFunfarm
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Re: Weird color [Re: Ashtray161]
    #27757928 - 04/30/22 05:33 PM (2 years, 8 months ago)

Will that all Races my next question that I have tons of questions what about injecting spores directly into grain bags it seems that there's mixed feelings about that it's enough to make me want to go do karate in the basement or the garage

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OfflineFunfarm
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Re: Weird color [Re: Funfarm]
    #27773809 - 05/12/22 10:43 AM (2 years, 8 months ago)

Well I did it this time, injected a whole 11 cc syringe of spores into a 2 pound bag. Frustration

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OfflineTight Lunchbox
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Re: Weird color [Re: Funfarm]
    #27773929 - 05/12/22 12:11 PM (2 years, 8 months ago)

Shooting spores into grains is not a safe practice. If you insist on doing so it is best to use as little solution as possible because it is highly unlikely that your syringe is 100% clean. You probably just wasted your bag of grain and your syringe. Good luck.


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OfflineB Traven
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Re: Weird color [Re: Tight Lunchbox]
    #27773961 - 05/12/22 12:22 PM (2 years, 8 months ago)

There's no mixed feelings, just a few ignorant loudmouths who have chosen this hill to die on and a bunch of old posts from when more of us didn't know any better...

But hey, you're headed down the same path many of us did- shooting spores into grain until we realized that it sucked.

Here's hoping you get lucky with that bag!


--------------------
Beware of advice- even this.

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