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OfflineFunfarm
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Color pissy yellow brown agar
    #27739921 - 04/18/22 09:41 AM (2 years, 8 months ago)

Couple weeks since inoculated with print and the agar plate is turning yellow brown, the mycellium remains white. Pics are top and bottom shot of plate.



Edited by Funfarm (04/18/22 09:52 AM)

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OfflineMrBlueshrooms
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Re: Weird color [Re: Funfarm]
    #27739938 - 04/18/22 09:50 AM (2 years, 8 months ago)

Those are nastey. The white looks like mold to me.

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OfflineDERRAYLD
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Re: Weird color [Re: Funfarm]
    #27739939 - 04/18/22 09:50 AM (2 years, 8 months ago)

Couple weeks you say?
Contaminated regardless the time.

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Invisible0001001
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Registered: 02/04/21
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Re: Weird color [Re: DERRAYLD]
    #27739941 - 04/18/22 09:51 AM (2 years, 8 months ago)

Throw them out and start over.

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OfflineBobbins
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Re: Weird color [Re: 0001001]
    #27739961 - 04/18/22 10:07 AM (2 years, 8 months ago)

Get behind me satan.


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OfflineFunfarm
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Re: Weird color [Re: Bobbins]
    #27739971 - 04/18/22 10:21 AM (2 years, 8 months ago)

Thank you for the quick responses. Yeah I'm quickly finding out the importance of having a still air box to work with spores and agar. My previous round I introduced gray colored agar plates to sterile rye seed bags I ordered. Inserting sliced agar thru slits in the top of the bags and then sealing them with micropore tape to only grow green slime.  Since s laminar flow hood isn't in the budget I'm going to park the attempts with agar & prints coupled with poor technique and go to less handling/contamination chances to injecting spores directly to bags inside a still air box.
Atleast experience with failed attemots. Longing for healthy mycellium on rye. Thanks again for the quick responses

Edited by Funfarm (04/18/22 10:27 AM)

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Invisible0001001
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Re: Weird color [Re: Funfarm]
    #27740153 - 04/18/22 12:44 PM (2 years, 8 months ago)

Quote:

Funfarm said:
Thank you for the quick responses. Yeah I'm quickly finding out the importance of having a still air box to work with spores and agar. My previous round I introduced gray colored agar plates to sterile rye seed bags I ordered. Inserting sliced agar thru slits in the top of the bags and then sealing them with micropore tape to only grow green slime.  Since s laminar flow hood isn't in the budget I'm going to park the attempts with agar & prints coupled with poor technique and go to less handling/contamination chances to injecting spores directly to bags inside a still air box.
Atleast experience with failed attemots. Longing for healthy mycellium on rye. Thanks again for the quick responses






You definitely need a still air or glove box at minimum.

Pouring or doing any agar work in open air is going to contaminate.

Spores can be dirty and you'll most likely have to transfer away from contams from your germ dishes even with using an S.A.B

Edited by 0001001 (04/18/22 12:46 PM)

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OfflineFunfarm
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Re: Weird color [Re: 0001001]
    #27740201 - 04/18/22 01:13 PM (2 years, 8 months ago)

Purchased a new clear tote and a 4 inch hole saw from the store. Going to order a new sterile bag of rye and spore syringe. Sterilize the needle with a torch, wipe the sealing port with alcohol. Ill do that inside the freshly constructed still aero box. I'm going to let the black spores float to the top of the syringe and just inject those couple of cc's with one poke and discard the rest of the liquid syringe. I'm not a fan of multiple pokes on one bag.

Edited by Funfarm (04/18/22 01:15 PM)

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Offlinedyel
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Re: Weird color [Re: Funfarm] * 1
    #27740203 - 04/18/22 01:17 PM (2 years, 8 months ago)

don't jab grain bags with spore syringes, use a bit of the liquid on agar then once you know it's clean you can use that for grain/lc

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OfflineFunfarm
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Re: Weird color [Re: Funfarm]
    #27740205 - 04/18/22 01:19 PM (2 years, 8 months ago)

Shout out to member Forrester who advised me to build a still air box in a previous thread concerning agar. Should of listened! I'm a hard headed fool, I thought really this stuff grows in nature, does it really have to be super clean and still? 3 attempts later..... ah yeah! What a noobie

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Offlinedyel
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Re: Weird color [Re: Funfarm]
    #27740215 - 04/18/22 01:24 PM (2 years, 8 months ago)

well... It does grow in nature but so is mold
we don't want anything to compete with our mushrooms for the food we are giving them
In nature its a free for all

edit: you should listen to Forrester, he never shoots from his ass, if he have your unswer he will tell you and if not he will tell you to wait for another opinion

Edited by dyel (04/18/22 01:28 PM)

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OfflineFunfarm
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Re: Weird color [Re: dyel]
    #27740216 - 04/18/22 01:25 PM (2 years, 8 months ago)

I'm nervous to work with agar now, was thinking clean syringe to sterile bag inside still box less chances for garbage

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OfflineiceNock


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Re: Weird color [Re: Funfarm]
    #27740219 - 04/18/22 01:28 PM (2 years, 8 months ago)

Quote:

Funfarm said:
Shout out to member Forrester who advised me to build a still air box in a previous thread concerning agar. Should of listened! I'm a hard headed fool, I thought really this stuff grows in nature, does it really have to be super clean and still? 3 attempts later..... ah yeah! What a noobie





In nature though the micro climates where they thrive are generally specific. This isn't like growing plants where people are opining about the best soil mix even though there are 500 different things you could use that would still work in the soil. In the sense of getting a living, clean culture and then getting it to thrive and still be clean on grain you have to at least look at it as attempting to keep it completely sterile and do everything towards that effect.

Once your jars are fully colonized though you're chilling and no longer have to be so concerned.


Edit : Working with agar is the lowest chance you have to end up with garbage. Spores to grain is generally a bad idea and if you are just wanting fruits and wanting to do it with your spore syringe you should do "PF Tek". If you want an easier way to get in to agar, I use Josex no-pour.

Edited by iceNock (04/18/22 01:32 PM)

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Offlinedyel
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Re: Weird color [Re: Funfarm]
    #27740222 - 04/18/22 01:30 PM (2 years, 8 months ago)

I understand your point but spore syringes are usually not clean, agar gives you a quick way to verify and/or clean the culture

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InvisibleMTZ
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Re: Weird color [Re: Funfarm]
    #27740238 - 04/18/22 01:40 PM (2 years, 8 months ago)

He's a good dude. He doesn't know it, but I've been hunting down what he advises in the gourmet section for a little over a year before I finally made an account.


--------------------
Rehears your death every morning and night. Only when you constantly live as though already a corpse will you find freedom in the martial way, and fulfill your duties without fault throughout your life. ~ The Book Of Samurai
........
I live in the void. I can go to the in between anytime I desire. I visit the spirt world.

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Invisible0001001
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Re: Weird color [Re: MTZ]
    #27740341 - 04/18/22 02:42 PM (2 years, 8 months ago)

Funfarm,

Careful with using a hole saw to not crack the plastic. When I'd build a S.A.B I used to just use a huge enpty can and heat it up then press holes in the plastic.

You shouldn't be intimidated by working with agar. Though using a spore syringe on agar isn't the preferred way to go about it, it can still be done. You just want to use the smallest drop possible. If you'd like to try working with swabs, I probably got a few extra sets of genetics - just shoot me a D.M

Edit:

Im assuming you have a scalpel and a torch or alcohol burner? If not you really need to aquire these things before proceeding with agar work.

Edited by 0001001 (04/18/22 02:44 PM)

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OfflineFunfarm
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Re: Weird color [Re: 0001001]
    #27740489 - 04/18/22 04:05 PM (2 years, 8 months ago)

Thank you 0010001, I think I'm going to try it again inside the s.a.b. that being said I'm going to wear a face mask gloves and lots of alcohol

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Invisible0001001
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Re: Weird color [Re: Funfarm]
    #27740534 - 04/18/22 04:33 PM (2 years, 8 months ago)

Quote:

Funfarm said:
Thank you 0010001, I think I'm going to try it again inside the s.a.b. that being said I'm going to wear a face mask gloves and lots of alcohol




Careful using a torch with alcohol fumes.

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OfflineFunfarm
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Re: Weird color [Re: 0001001]
    #27742366 - 04/19/22 05:02 PM (2 years, 8 months ago)

Kaboom! So spray the wiping towelettes on the outside of the box, being careful of residual fumes.  Grandma thinks I need to learn to crawl before walk, and not work with the agar right away but consider a all in one bag with single syringe???

Edited by Funfarm (04/19/22 05:06 PM)

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InvisibleWyoMX
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Re: Weird color [Re: Funfarm] * 1
    #27742374 - 04/19/22 05:07 PM (2 years, 8 months ago)

Syringe to grain is one of the worst ways to grow. Crawling before walking would be learning agar. It seems intimidating but once you do it it's not very hard and actually pretty fun.

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