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OfflineFunfarm
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Color pissy yellow brown agar
    #27739921 - 04/18/22 09:41 AM (2 years, 8 months ago)

Couple weeks since inoculated with print and the agar plate is turning yellow brown, the mycellium remains white. Pics are top and bottom shot of plate.



Edited by Funfarm (04/18/22 09:52 AM)

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OfflineMrBlueshrooms
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Re: Weird color [Re: Funfarm]
    #27739938 - 04/18/22 09:50 AM (2 years, 8 months ago)

Those are nastey. The white looks like mold to me.

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OfflineDERRAYLD
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Re: Weird color [Re: Funfarm]
    #27739939 - 04/18/22 09:50 AM (2 years, 8 months ago)

Couple weeks you say?
Contaminated regardless the time.

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Invisible0001001
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Re: Weird color [Re: DERRAYLD]
    #27739941 - 04/18/22 09:51 AM (2 years, 8 months ago)

Throw them out and start over.

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OfflineBobbins
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Re: Weird color [Re: 0001001]
    #27739961 - 04/18/22 10:07 AM (2 years, 8 months ago)

Get behind me satan.


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OfflineFunfarm
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Re: Weird color [Re: Bobbins]
    #27739971 - 04/18/22 10:21 AM (2 years, 8 months ago)

Thank you for the quick responses. Yeah I'm quickly finding out the importance of having a still air box to work with spores and agar. My previous round I introduced gray colored agar plates to sterile rye seed bags I ordered. Inserting sliced agar thru slits in the top of the bags and then sealing them with micropore tape to only grow green slime.  Since s laminar flow hood isn't in the budget I'm going to park the attempts with agar & prints coupled with poor technique and go to less handling/contamination chances to injecting spores directly to bags inside a still air box.
Atleast experience with failed attemots. Longing for healthy mycellium on rye. Thanks again for the quick responses

Edited by Funfarm (04/18/22 10:27 AM)

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Invisible0001001
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Re: Weird color [Re: Funfarm]
    #27740153 - 04/18/22 12:44 PM (2 years, 8 months ago)

Quote:

Funfarm said:
Thank you for the quick responses. Yeah I'm quickly finding out the importance of having a still air box to work with spores and agar. My previous round I introduced gray colored agar plates to sterile rye seed bags I ordered. Inserting sliced agar thru slits in the top of the bags and then sealing them with micropore tape to only grow green slime.  Since s laminar flow hood isn't in the budget I'm going to park the attempts with agar & prints coupled with poor technique and go to less handling/contamination chances to injecting spores directly to bags inside a still air box.
Atleast experience with failed attemots. Longing for healthy mycellium on rye. Thanks again for the quick responses






You definitely need a still air or glove box at minimum.

Pouring or doing any agar work in open air is going to contaminate.

Spores can be dirty and you'll most likely have to transfer away from contams from your germ dishes even with using an S.A.B

Edited by 0001001 (04/18/22 12:46 PM)

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OfflineFunfarm
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Re: Weird color [Re: 0001001]
    #27740201 - 04/18/22 01:13 PM (2 years, 8 months ago)

Purchased a new clear tote and a 4 inch hole saw from the store. Going to order a new sterile bag of rye and spore syringe. Sterilize the needle with a torch, wipe the sealing port with alcohol. Ill do that inside the freshly constructed still aero box. I'm going to let the black spores float to the top of the syringe and just inject those couple of cc's with one poke and discard the rest of the liquid syringe. I'm not a fan of multiple pokes on one bag.

Edited by Funfarm (04/18/22 01:15 PM)

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Re: Weird color [Re: Funfarm] * 1
    #27740203 - 04/18/22 01:17 PM (2 years, 8 months ago)

don't jab grain bags with spore syringes, use a bit of the liquid on agar then once you know it's clean you can use that for grain/lc

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OfflineFunfarm
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Re: Weird color [Re: Funfarm]
    #27740205 - 04/18/22 01:19 PM (2 years, 8 months ago)

Shout out to member Forrester who advised me to build a still air box in a previous thread concerning agar. Should of listened! I'm a hard headed fool, I thought really this stuff grows in nature, does it really have to be super clean and still? 3 attempts later..... ah yeah! What a noobie

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Re: Weird color [Re: Funfarm]
    #27740215 - 04/18/22 01:24 PM (2 years, 8 months ago)

well... It does grow in nature but so is mold
we don't want anything to compete with our mushrooms for the food we are giving them
In nature its a free for all

edit: you should listen to Forrester, he never shoots from his ass, if he have your unswer he will tell you and if not he will tell you to wait for another opinion

Edited by dyel (04/18/22 01:28 PM)

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OfflineFunfarm
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Re: Weird color [Re: dyel]
    #27740216 - 04/18/22 01:25 PM (2 years, 8 months ago)

I'm nervous to work with agar now, was thinking clean syringe to sterile bag inside still box less chances for garbage

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Re: Weird color [Re: Funfarm]
    #27740219 - 04/18/22 01:28 PM (2 years, 8 months ago)

Quote:

Funfarm said:
Shout out to member Forrester who advised me to build a still air box in a previous thread concerning agar. Should of listened! I'm a hard headed fool, I thought really this stuff grows in nature, does it really have to be super clean and still? 3 attempts later..... ah yeah! What a noobie





In nature though the micro climates where they thrive are generally specific. This isn't like growing plants where people are opining about the best soil mix even though there are 500 different things you could use that would still work in the soil. In the sense of getting a living, clean culture and then getting it to thrive and still be clean on grain you have to at least look at it as attempting to keep it completely sterile and do everything towards that effect.

Once your jars are fully colonized though you're chilling and no longer have to be so concerned.


Edit : Working with agar is the lowest chance you have to end up with garbage. Spores to grain is generally a bad idea and if you are just wanting fruits and wanting to do it with your spore syringe you should do "PF Tek". If you want an easier way to get in to agar, I use Josex no-pour.

Edited by iceNock (04/18/22 01:32 PM)

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Re: Weird color [Re: Funfarm]
    #27740222 - 04/18/22 01:30 PM (2 years, 8 months ago)

I understand your point but spore syringes are usually not clean, agar gives you a quick way to verify and/or clean the culture

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Re: Weird color [Re: Funfarm]
    #27740238 - 04/18/22 01:40 PM (2 years, 8 months ago)

He's a good dude. He doesn't know it, but I've been hunting down what he advises in the gourmet section for a little over a year before I finally made an account.


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Re: Weird color [Re: MTZ]
    #27740341 - 04/18/22 02:42 PM (2 years, 8 months ago)

Funfarm,

Careful with using a hole saw to not crack the plastic. When I'd build a S.A.B I used to just use a huge enpty can and heat it up then press holes in the plastic.

You shouldn't be intimidated by working with agar. Though using a spore syringe on agar isn't the preferred way to go about it, it can still be done. You just want to use the smallest drop possible. If you'd like to try working with swabs, I probably got a few extra sets of genetics - just shoot me a D.M

Edit:

Im assuming you have a scalpel and a torch or alcohol burner? If not you really need to aquire these things before proceeding with agar work.

Edited by 0001001 (04/18/22 02:44 PM)

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OfflineFunfarm
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Re: Weird color [Re: 0001001]
    #27740489 - 04/18/22 04:05 PM (2 years, 8 months ago)

Thank you 0010001, I think I'm going to try it again inside the s.a.b. that being said I'm going to wear a face mask gloves and lots of alcohol

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Invisible0001001
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Re: Weird color [Re: Funfarm]
    #27740534 - 04/18/22 04:33 PM (2 years, 8 months ago)

Quote:

Funfarm said:
Thank you 0010001, I think I'm going to try it again inside the s.a.b. that being said I'm going to wear a face mask gloves and lots of alcohol




Careful using a torch with alcohol fumes.

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OfflineFunfarm
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Re: Weird color [Re: 0001001]
    #27742366 - 04/19/22 05:02 PM (2 years, 8 months ago)

Kaboom! So spray the wiping towelettes on the outside of the box, being careful of residual fumes.  Grandma thinks I need to learn to crawl before walk, and not work with the agar right away but consider a all in one bag with single syringe???

Edited by Funfarm (04/19/22 05:06 PM)

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Re: Weird color [Re: Funfarm] * 1
    #27742374 - 04/19/22 05:07 PM (2 years, 8 months ago)

Syringe to grain is one of the worst ways to grow. Crawling before walking would be learning agar. It seems intimidating but once you do it it's not very hard and actually pretty fun.

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OfflineFunfarm
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Re: Weird color [Re: WyoMX]
    #27743379 - 04/20/22 10:02 AM (2 years, 8 months ago)

Maybe I'm getting bad Agar plates thru order? Can anyone suggest a good place for agar plates? I checked the trusted vendors but didnt see any?

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OfflineFunfarm
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Re: Weird color [Re: Funfarm]
    #27743433 - 04/20/22 10:39 AM (2 years, 8 months ago)

420!
Looking for good agar plates to order

Edited by Funfarm (04/20/22 10:40 AM)

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OfflineFunfarm
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Re: Weird color [Re: Funfarm]
    #27744086 - 04/20/22 06:17 PM (2 years, 8 months ago)

Grandma is laughing at me, she says they look like urinal cakes. The mold is growing right where the spores are.


Edited by Funfarm (04/20/22 06:19 PM)

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Invisible0001001
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Re: Weird color [Re: Funfarm] * 1
    #27744218 - 04/20/22 07:32 PM (2 years, 8 months ago)

Just make your own agar plates?!?!?!??!?!?

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OfflineFunfarm
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Re: Weird color [Re: 0001001]
    #27745240 - 04/21/22 01:30 PM (2 years, 8 months ago)

I did see a pretty good receipe an process on line involving using a glass liquor bottle. Looked like all the Ingredients could be obtained. Honestly it was my fault not the plates

Edited by Funfarm (04/21/22 02:35 PM)

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OfflineFunfarm
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Re: Weird color [Re: Funfarm]
    #27754754 - 04/28/22 09:05 AM (2 years, 8 months ago)

Hello I was wondering how long it takes your agar plates 2 become fully colonised with mycelium once inoculated?

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Re: Weird color [Re: Funfarm]
    #27754826 - 04/28/22 09:46 AM (2 years, 8 months ago)

Quote:

Funfarm said:
Hello I was wondering how long it takes your agar plates 2 become fully colonised with mycelium once inoculated?




It depends on a lot of different factors, but full colonization is rarely your goal anyway.

I'd say you should have spore germination by about the 2 week mark. Then you should really be off to the races- immediate transfer of germinated patches, and then sequential transfers about once a week.

Failure rates can be high on agar, depending on your plate prep, spore solution and sterile techniques. But the great advantage is that you can easily start lots of plates until you find some winners.

That mold you have might have come straight from the spore syringe. Or, the liquid you  squirted encouraged its growth. Keep trying, you'll get it!

I have found that my failure rate dropped once I started streaking spore prints onto plates, rather than using syringes. You could give that a try if you get frustrated.


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OfflineFunfarm
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Re: Weird color [Re: B Traven]
    #27754966 - 04/28/22 11:26 AM (2 years, 8 months ago)

Hey thanks for the great response I appreciate it

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Re: Weird color [Re: Funfarm]
    #27755737 - 04/28/22 09:06 PM (2 years, 8 months ago)

So heres the next line of question.... what causes an agar plate to stall in growth? Has the plate run out of sugar fuel, all being absorbed to promote what was rapid colonization only to stall in mycellium, is the agar plate running out of fuel? Perhaps remove the healthy thick percentage of thick mycellium to a new fresh agar plate with fresh fuel to further substantiate new aggressive colonization? Is there validity to this new ponder? Hmmm? Rapid growth, no more growth.... biopsy old plate to new fresh pour plate, does the mycellium then continue growth pattern? Inquiring mind wants to know, to much vodka and sativa dom psychoactive thc has mindless fool wondering. Glorious triumphant, happy growth and trips for you.

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Re: Weird color [Re: Funfarm]
    #27756161 - 04/29/22 05:55 AM (2 years, 8 months ago)

Mycelium will typically colonize all of a plate if it has the chance to, and then often start pinning. Or, if left sitting long enough, it'll dry out. Plates that I forget about, if they're not contaminated, tend to either dry out or kick out little mini fruits that dump spores all over them.

Colonizing all of the available substrate, and then using up most of the available nutrients, is a major trigger for fruiting, because that's when it's time to kick out spores to colonize new locations.

If you get a plate that stalls out before fully colonizing, then it's likely an issue with contamination.

But again, you're usually not trying for full colonization. You're trying to get transfers before the mycelium is hopelessly intertwined with any potential contaminants. Only on plates that you were using for inoculation, or plates you wanted to grow pins on, would you want full colonization.

When you start collecting and obsessing over plates, that's when the real geek-out begins πŸ‘

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OfflineFunfarm
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Re: Weird color [Re: B Traven]
    #27756313 - 04/29/22 09:09 AM (2 years, 8 months ago)

So the name of the game is constant agar 2 agar transfer of healthy mycellium chunks?

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Re: Weird color [Re: Funfarm]
    #27756315 - 04/29/22 09:12 AM (2 years, 8 months ago)

Quote:

Funfarm said:
So the name of the game is constant agar 2 agar transfer of healthy mycellium chunks?




That's indeed the name of the game good sir, madam, or human.


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OfflinehaniblecterS
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Re: Weird color [Re: WyoMX]
    #27757282 - 04/30/22 07:32 AM (2 years, 8 months ago)

gotta disagree. messing with a whole 'nother system to avoid some jar contams makes no sense. truly believe it does new people a diservice telling them agar is the only way when MS syringe will get it done.

but ya, SAB is the only way. hole saws work but you have to be super slow, almost to the point of melting the plastic.

side note, cut the top out and duct tape some plexiglass to it. itll help SOO MUCH with visibility.


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Re: Weird color [Re: haniblecter]
    #27757493 - 04/30/22 11:29 AM (2 years, 8 months ago)

Quote:

haniblecter said:
gotta disagree. messing with a whole 'nother system to avoid some jar contams makes no sense. truly believe it does new people a diservice telling them agar is the only way when MS syringe will get it done.

but ya, SAB is the only way. hole saws work but you have to be super slow, almost to the point of melting the plastic.

side note, cut the top out and duct tape some plexiglass to it. itll help SOO MUCH with visibility.




You're dead wrong man. Agar is needed in this hobby and the quicker you learn it the better. Agar is the only reliable way to go with MS syringes.

OP, learn agar.


--------------------
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Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086:elmo:
"You never have to prove the fool a fool, just let them speak."
Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED.
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Re: Weird color [Re: haniblecter]
    #27757569 - 04/30/22 12:41 PM (2 years, 8 months ago)

Quote:

haniblecter said:
gotta disagree. messing with a whole 'nother system to avoid some jar contams makes no sense. truly believe it does new people a diservice telling them agar is the only way when MS syringe will get it done.






Agar isn't the only way by any means- PF tek usually works fine with straight spore syringes, if you want to keep it simple.

But I really wish I had been more strongly discouraged from injecting spores into grain jars. It wasted a lot of my time, and taught me very little.


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Re: Weird color [Re: B Traven]
    #27757585 - 04/30/22 01:09 PM (2 years, 8 months ago)

That's true, I didnt think about PF tek. That's the only scenario I can think of where agar isnt needed


--------------------
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Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086:elmo:
"You never have to prove the fool a fool, just let them speak."
Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED.
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OfflineFunfarm
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Re: Weird color [Re: Ashtray161]
    #27757928 - 04/30/22 05:33 PM (2 years, 8 months ago)

Will that all Races my next question that I have tons of questions what about injecting spores directly into grain bags it seems that there's mixed feelings about that it's enough to make me want to go do karate in the basement or the garage

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Re: Weird color [Re: Funfarm]
    #27773809 - 05/12/22 10:43 AM (2 years, 8 months ago)

Well I did it this time, injected a whole 11 cc syringe of spores into a 2 pound bag. Frustration

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Re: Weird color [Re: Funfarm]
    #27773929 - 05/12/22 12:11 PM (2 years, 8 months ago)

Shooting spores into grains is not a safe practice. If you insist on doing so it is best to use as little solution as possible because it is highly unlikely that your syringe is 100% clean. You probably just wasted your bag of grain and your syringe. Good luck.


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Re: Weird color [Re: Tight Lunchbox]
    #27773961 - 05/12/22 12:22 PM (2 years, 8 months ago)

There's no mixed feelings, just a few ignorant loudmouths who have chosen this hill to die on and a bunch of old posts from when more of us didn't know any better...

But hey, you're headed down the same path many of us did- shooting spores into grain until we realized that it sucked.

Here's hoping you get lucky with that bag!


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Re: Weird color [Re: Funfarm]
    #27774052 - 05/12/22 01:27 PM (2 years, 8 months ago)

You know the agar starts out super fast but in my opinion with no s.a.b. nor laminar air flow I think opening a bag of grain in a Petri dish to fish out the eggar exposes the mycelium to the open-air contaminants not to mention the three inches of mold I found in one of my windowsill Corners last night as I punch myself in the head only leads to the failed attempts that I've had working with agar in Rye seed so in frustration I thought that by heating the needle and pushing it in through the self-healing port that I might have better luck thank you for the kind words and happy trips

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Re: Weird color [Re: Funfarm]
    #27774146 - 05/12/22 02:27 PM (2 years, 8 months ago)

No SAB?

You gotta get a SAB.

Start with saran wrap and a cardboard box if you have to, but at least work in still air!


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Re: Weird color [Re: B Traven]
    #27774399 - 05/12/22 05:32 PM (2 years, 8 months ago)

On my next paycheck next week I'm getting another clear tote and I found a 4-in hole saw in a thrift shop so I got that going for me. I don't think I'm going to do anymore hobby enthusiasm with fungi until I get the clear tote and some holes in it. Grandma even suggested ordering one of those fancy kits from overseas where you just add water and it grows inside of a Ziploc bag. She said instead of adding the water just take the mycelium that's in the tupperware bowl and throw it in with some rye seed. Kind of a silly way to go about propagation but I guess you got to start somewhere. So I tells Grandma we're going to give it another shot with a still air box I got to figure out how to do this on my own. I'm thinking with the mold in the corner of the window and trying to do this with all the funky nasty in the air that's a huge part of the problem thanks for your responses have a super night

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Re: Weird color [Re: Funfarm]
    #27780033 - 05/16/22 09:45 AM (2 years, 7 months ago)

Got the 2 pound rye sitting in the hot water heater closet a nice 70 degrees constant. The bag has white mold all over the bottom of it growing upwards in clumps. I was hoping to get a uline pulse sealer online and do g2g transfer. I wanted to get kitchen cleaning gloves that come up high on the forearm, and transfer the contents of the inoculated 2 pound bag over a quantity of 4 two pound spawn bags. Basically a large handful of colonized grain to each bag in a attempt to keep perpetual propagation of mycellium going. The idea was 5 to 8 pounds colonized rye seed layered with 10 to 20 pounds of prepared manure in a clear tote. Again that was the plan but I can't seem to grow anything but pissy yellow brown agar and that cotton mold ingrained in the rye seed between the inner wall of the poly bag. Grandma keeps laughing at me, and I swear if she keeps mocking me, I'm going to quit buying her beer and vodka. Still air box kung fu is in order, especially if I ever do g2g. Have a glorious magnificent day.

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Re: Weird color [Re: Funfarm]
    #27780043 - 05/16/22 10:02 AM (2 years, 7 months ago)

A 4" hole is too small unless you have TINY arms.  A 6-8" hole is much more effective as your arms don't act like plungers pushing air around and they are more comfortable to use.  Many people use an old coffee or bulk food can and heat it up and push it through the plastic for a smooth edged larger hole.

Trust me, the mold in your home is not the issue.  All that matters is that the area inside the SAB with your dishes/bags stays clean.

I highly recommend AGAINST using a spore to grain spawn source for G2G.  You will very likely have some backlevel of contamination in that syringe and going G2G will allow it to expand faster than the mycelium.

IMO if you're not effectively using agar for your grain inoculation, you are fighting the odds for success.  Once you have an actually clean culture everything else falls in place.

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Re: Weird color [Re: PBJ710]
    #27780164 - 05/16/22 11:57 AM (2 years, 7 months ago)

How do identify a clean culture? In guessing uniform growth?

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Re: Weird color [Re: Funfarm]
    #27780503 - 05/16/22 03:55 PM (2 years, 7 months ago)

Uniform growth centered on your inoculation point without anything else growing on the plate is a pretty safe bet.  It's still possible to have molds get meshed with the mycelium and get uniform growth, but the growth itself looks a bit more disorganized than typical rhizo/tomatose growth.

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Re: Weird color [Re: PBJ710]
    #27782868 - 05/18/22 11:18 AM (2 years, 7 months ago)

Right on, and I tell you unfortunately that is not what I see when working with plates, typically uneven with green mold balloon heads, with the agar turning either gray or yellow.

Heres my question, can I take to separate agar plates, inoculate both of them and then sandwich them together spore side to spore sine and allow them to grow together in one taped up plate? I'm thinking the spores won't dry out and it would be embedded and would have plenty of food to grow?  Thoughts?

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Re: Weird color [Re: Funfarm]
    #27782891 - 05/18/22 11:42 AM (2 years, 7 months ago)

You're supposed to transfer away from contams, not hide from them.  Your spores are not drying out nor starving for food, but if you agar is too hard it can make it difficult for the mycelium to get started.  Try 7.5-8g agar per 500ml water on the next round and use a sterile loop to streak the spores to the plate.  You need to know where you inoculated the plate so that you can tell where good/bad growth is and then get good growth off those spore plates ASAP.  After you have a transfer from that plate you can let it grow out and start selecting sectors for the most aggressive growth.

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Re: Weird color [Re: PBJ710]
    #27782946 - 05/18/22 12:25 PM (2 years, 7 months ago)

I'd recommend watching some youtube videos to at least get the very basics of working with agar down.

Follow trusted cultivators on here and continue to educate yourself. With the right information & techniques you'll atleast have a chance at success.


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Re: Weird color [Re: 0001001]
    #27783227 - 05/18/22 04:02 PM (2 years, 7 months ago)

Right on it's like plate agar biopsies selectively taking the best genetics from the plate and puzzling it on and further propagation for better results. I'll check out some more YouTube videos for sure and in the meantime I guess I'm going to have to get online and order some more agar I know some guys have suggested making my own but I'm not at that point yet got to still air box so we're doing better guys and girls have glorious day

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Re: Weird color [Re: Funfarm]
    #27783297 - 05/18/22 04:54 PM (2 years, 7 months ago)

I made agar in a SAB for a year before I decided I was serious enough into the hobby to get an FFU.  :shrug:  I know making agar sounds super complicated when getting started, but it's really alot easier than it seems.  You can do whats called 'no pour' plates in PP5 containers if you're not confident on your SAB technique pouring them.

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Re: Weird color [Re: Funfarm]
    #27783341 - 05/18/22 05:39 PM (2 years, 7 months ago)

Quote:

Funfarm said:
Got the 2 pound rye sitting in the hot water heater closet a nice 70 degrees constant. The bag has white mold all over the bottom of it growing upwards in clumps. I was hoping to get a uline pulse sealer online and do g2g transfer. I wanted to get kitchen cleaning gloves that come up high on the forearm, and transfer the contents of the inoculated 2 pound bag over a quantity of 4 two pound spawn bags. Basically a large handful of colonized grain to each bag in a attempt to keep perpetual propagation of mycellium going. The idea was 5 to 8 pounds colonized rye seed layered with 10 to 20 pounds of prepared manure in a clear tote. Again that was the plan but I can't seem to grow anything but pissy yellow brown agar and that cotton mold ingrained in the rye seed between the inner wall of the poly bag. Grandma keeps laughing at me, and I swear if she keeps mocking me, I'm going to quit buying her beer and vodka. Still air box kung fu is in order, especially if I ever do g2g. Have a glorious magnificent day.




just wanted to say this will end in failure, even if you wipe iso on the gloves they will be too dirty to keep spawn clean. you need to use a sterile instrument. working with bags inside an SAB is hard & i would recommend moving to jars if you want to go the G2G route. that way you can just dump grains into receiving jars without having to use something to physically remove them from the master jar/bag.

agar is really easy & i recommend you try it out, even if you’re intimidated. it’s very noob friendly & no pours are perfect for SAB work. you’ll get the hang of it really quick. here is a really good beginner agar tek to follow


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Re: Weird color [Re: hazyhorse]
    #27783345 - 05/18/22 05:44 PM (2 years, 7 months ago)

Thanks for the link I think you're right it will end in Failure so I'm going to check this no poor a gar out it sounds like something that we can work with good stuff good stuff. If I can say this, if moldy containment was the name of the game, I'd be spot on.

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Re: Weird color [Re: Funfarm]
    #27796380 - 05/28/22 12:03 PM (2 years, 7 months ago)

Since I had a hard time with agar plates, I'm going to try a single 5 pound bag of sterilized rye seed and 11 cc's of a single  multiple spore syringe. My grandma is in the kitchen smoking cigs and sipping vodka screaming Failure! Going to see top gun tom cruise taking my breath away f1ying thru the skies with moldy shrooms.

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Re: Weird color [Re: Funfarm]
    #27796388 - 05/28/22 12:17 PM (2 years, 7 months ago)

spore to grain bag is a pretty bad idea, it fails more often than it works. PF tek will probably be your best bet if you want to forgo agar


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Re: Weird color [Re: hazyhorse]
    #27796408 - 05/28/22 12:38 PM (2 years, 7 months ago)

The great thing about having a hard time with agar is that you can pretty easily just keep trying until you get it right, with minimal time and expense. Going bigger when you can't get agar nailed down is not the right direction.

Do you want one random grow that you get lucky on after who knows how many failures, or do you want to know how to grow more reliably for the future?

Do you want random crapshoot genetics, or do you want some control over what your final harvest will look like?


Seriously, if you're feeling too frustrated to continue with agar right now then just do PF cakes. They'll also give you clone-able and print-able fruits, which you can maybe use to play around with agar further on down the line.


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Re: Weird color [Re: B Traven]
    #27796415 - 05/28/22 12:45 PM (2 years, 7 months ago)

:whathesaid:

agar isn’t hard once you get the hang of it. what failures did you have? maybe we can help trouble shoot


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Re: Weird color [Re: hazyhorse]
    #27796627 - 05/28/22 04:36 PM (2 years, 7 months ago)

Basically the agar color turned gray one time and then on the second attempt to turn yellow it grew mycelium and it also grew the green mold bubbleheads. I think I probably am getting off base from the original topic of the yellow pissy Brown haggar that I had the second time maybe I should start a new thread concerning multi Spore syringe and rye seed? Thank goodness for the help I get here.  I've had a couple failed attempts at injecting bags to the first time I did four of the one pounders rye seed with multi Spore syringe got nothing and then the second time I tried doing Uncle Ben's rice bags and got absolutely nothing. I thought I was on the right track when I cut up that agar that it turn to Gray and put it in a rye seed bag that was sterile of course opening it up to contaminant and waiting 3 weeks to find absolutely nothing but a bunch of dust so against Grandma's best wishes we're going to try a single five pounder that I ordered and squirt the  whole 11 cc's into it. Grandma told me to order another Spore print and just put it in a jar of popcorn seeds I'll try anything at this point. I think I'm going to go to the library and check out a book on fungus and see about operating with p.f. Tek.

Edited by Funfarm (05/28/22 05:28 PM)

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Re: Weird color [Re: Funfarm]
    #27796652 - 05/28/22 04:47 PM (2 years, 7 months ago)

are you making the agar yourself? or are you buying it premade? if you have a pressure cooker, jars are better to work with inside SABs. bags are really difficult if you aren’t in front of a flowhood


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Re: Weird color [Re: hazyhorse]
    #27796736 - 05/28/22 05:57 PM (2 years, 7 months ago)

Basically what I set up was 6 mil polypropylene plastic containment on a shower rod up against the ceiling that drops down beneath the bathtub then I sit in the bathtub with a board shelf across the top and put my plastic tote on
It upside down that I punched holes in with a big V8 can. I get in the tub gingerly and have Grandma very slowly shut the bathroom door and I'll wait about 30 minutes in the tub for all the dust settle It was the best laboratory setting I could come up with. Then i squirt everything down with bleach before I get in it and I sit in there with a respirator on I haven't got to the point of agar pours yet I just simply want to see some mycellium colony. I'd like to get all the way through one tote lining it with the sterile manure and healthy seeds Pond before I get into doing any type of eger work

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