Home | Community | Message Board

MushroomCube.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Jump to first unread post Pages: 1
Offlineangryteabag
Stranger
Registered: 06/08/14
Posts: 21
Last seen: 2 years, 8 months
Can isopropyl alcohol vapours kill mycelium?
    #27113352 - 12/29/20 12:54 AM (3 years, 1 month ago)

I am thinking about doing an experiment in a glove box, but I want to be overzealous with the isopropyl alcohol.

If I spray down the inside with the alcohol and leave it wet inside the container while working with mycelium, assuming the mycelium never comes in contact with the liquid isopropyl alcohol and is only exposed to the atmosphere (containing vapors), will the mycelium die?


Extras: Filter Print Post Top
OfflineLeon ForestFacebookDiscord
Male


Registered: 02/11/13
Posts: 574
Last seen: 1 month, 20 days
Re: Can isopropyl alcohol vapours kill mycelium? [Re: angryteabag] * 1
    #27113363 - 12/29/20 01:17 AM (3 years, 1 month ago)

I would use water with a drop of soap in it for your sab walls not iso.


Extras: Filter Print Post Top
OfflineZakkery
I'm a teapot User Gallery


Registered: 10/15/18
Posts: 293
Loc: UK Flag
Last seen: 12 days, 15 hours
Re: Can isopropyl alcohol vapours kill mycelium? [Re: Leon Forest]
    #27113483 - 12/29/20 04:42 AM (3 years, 1 month ago)

I'm curious what your experiment is? Does it require a glove box?

If you just need a clean environment to work in, the best advice is to remove the gloves and use it as a SAB. This will be more effective than an ISO soaked glove box for clean procedures.


Extras: Filter Print Post Top
OfflineEldest Oyster
Stranger
Registered: 05/14/19
Posts: 8
Last seen: 1 year, 5 months
Re: Can isopropyl alcohol vapours kill mycelium? [Re: Zakkery]
    #27728451 - 04/10/22 08:14 AM (1 year, 10 months ago)

OK, I am thinking of an experiment to reduce condensation when I pour agar plates.  It involves reducing the water vapor in the air inside the SAB.  Replacing the water with alcohol inside the SAB (no open flame of course)So I had the same question about the vapor and the Mycelium.


Extras: Filter Print Post Top
InvisibleLotKid
Never.Trust.A.Prankster
Male User Gallery


Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
Trusted Cultivator
Re: Can isopropyl alcohol vapours kill mycelium? [Re: Eldest Oyster] * 1
    #27728560 - 04/10/22 09:52 AM (1 year, 10 months ago)

:justno:


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:


Extras: Filter Print Post Top
Offlinechris77
Archaic Revivalist
 User Gallery


Registered: 01/17/22
Posts: 1,925
Last seen: 12 hours, 22 minutes
Re: Can isopropyl alcohol vapours kill mycelium? [Re: LotKid]
    #27728593 - 04/10/22 10:28 AM (1 year, 10 months ago)

Quote:

LotKid said:
:justno:



as much i am pro experimentation, i think that lotkid is spot on..


--------------------
the observer is the observed
            j. krishnamurti


Extras: Filter Print Post Top
Offlinethe man
still masked
Other User Gallery


Registered: 08/12/99
Posts: 6,681
Loc: C A N A D A
Last seen: 18 days, 5 hours
Re: Can isopropyl alcohol vapours kill mycelium? [Re: chris77]
    #27728723 - 04/10/22 11:34 AM (1 year, 10 months ago)

yup, iso absorbs water but not THAT much. remember 99% iso is a thing, if it absorbed MAD water it would drop to 80% pretty quick. it helps keep water from dropping out of gasoline in freezing temps but thats MUCH different than abosrbing many ml of condensation FROM INSIDE a petri dish..long and short no it wont work and yes if enough iso to try and get a osmotic suck of water it will kill myc... thats why it kills myc is it sucks out the water :wink: (or destroys lipid cell walls of bac or virus)

if want to do something try to use TEMPERATURE to prevent condensation. pan of warm water over lid sof petri or just remember petris are poured daily with no or little condensation.


Extras: Filter Print Post Top
OfflineEldest Oyster
Stranger
Registered: 05/14/19
Posts: 8
Last seen: 1 year, 5 months
Re: Can isopropyl alcohol vapours kill mycelium? [Re: the man]
    #27730153 - 04/11/22 11:19 AM (1 year, 10 months ago)

Ah, a clarification.  My concept is that I will spray iso on the table and inside of SAB instead of water.  The stuff floating in the air would stick to the alcohol instead of the water with a bit of soap in it that I usually use.

The humidity of the air inside the SAB will be lower, as there is not the mist of water on the surfaces evaporating into the air and making it more humid. 

I would also warm the glass petri dishes and cool the agar to the point it is almost congealing.  If I can dehumidify t he room air somehow I'll do that. 

If it all works, I'll post a tek!


Extras: Filter Print Post Top
InvisibleLotKid
Never.Trust.A.Prankster
Male User Gallery


Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
Trusted Cultivator
Re: Can isopropyl alcohol vapours kill mycelium? [Re: Eldest Oyster]
    #27730168 - 04/11/22 11:45 AM (1 year, 10 months ago)

:justno:


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:


Extras: Filter Print Post Top
Jump to top Pages: 1


Similar ThreadsPosterViewsRepliesLast post
* 1. does lysol \ alcohol kill mycelium \ spores ? growin 9,489 9 06/29/02 07:36 AM
by DrGoodtrips
* Ethanol VS Isopropyl Alcohol holio1 4,085 7 03/30/04 02:08 PM
by thenetherworld
* Anyone a fan of Isopropyl Alcohol for Sterility?? Viking 1,383 3 01/14/03 02:35 AM
by Sorted
* Mass mycelium extraction tek flatalbert 25,072 18 09/17/19 12:07 AM
by bodhisatta
* Alcohol-sprayed glovebox: how to sterilize scalpel? vzalkaloid 4,008 3 07/14/03 01:14 PM
by vzalkaloid
* Alcohol? Bushdoctor 1,289 14 10/22/03 03:28 AM
by Shamanorrhea
* 99 Percent Rubbing Alcohol. Signo 5,233 9 11/02/03 03:20 PM
by daussaulit
* Re: Alcohol 80% better than 96%!!
( 1 2 all )
paddoholland 5,223 25 03/15/01 09:40 PM
by Shroomzilla

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,688 topic views. 38 members, 251 guests and 57 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.005 seconds on 12 queries.