Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   OlympusMyco.com Olympus Myco Grow Bags   Mushroom-Hut Liquid Cultures   PhytoExtractum Kratom Powder for Sale   MagicBag.co All-In-One Bags That Don't Suck

Jump to first unread post Pages: 1
Invisibleredrum
Aspiring Mycologist
 User Gallery

Registered: 11/03/15
Posts: 169
Tissue to agar issue
    #27714391 - 03/30/22 05:21 PM (2 years, 1 month ago)

On 2/22/22, I took a tissue sample from the first and also biggest fruit on one of my BRF cakes and put it to agar. On 3/02/22, I had absolutely no growth on the agar but had mycelium growing on the fruit itself. With my scalpel, I flipped and moved the sample over a bit so the mycelium on the fruit was touching the agar. The same day, I also transferred a wedge from a plate I knocked up with a MS syringe back in January to another plate.

The first pic is from the MS syringe agar to agar transfer. The second is from the tissue sample. Its been over a month since the tissue transfer. Why is there a problem with growth for the tissue? Ive done this a few times in the past. I am seeing no contams at all. Even a few of my MS syringe to agar plates thrived quickly.




Extras: Filter Print Post Top
OfflineDERRAYLD
Constructus
Male User Gallery
Registered: 05/13/02
Posts: 10,170
Loc: South Africa
Last seen: 11 hours, 26 minutes
Trusted Cultivator
Re: Tissue to agar issue [Re: redrum]
    #27714914 - 03/31/22 01:48 AM (2 years, 1 month ago)

This should be posted in the cultivation forum to get more views, it's not remotely advanced.

Your pictures are too blurry to see anything,  generally speaking slow growth for me is attributes to bacterial competition.

Extras: Filter Print Post Top
Invisibleredrum
Aspiring Mycologist
 User Gallery

Registered: 11/03/15
Posts: 169
Re: Tissue to agar issue [Re: redrum]
    #27714940 - 03/31/22 03:19 AM (2 years, 1 month ago)

How can I get this moved instead of creating a dupe thread?

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag   OlympusMyco.com Olympus Myco Grow Bags   Mushroom-Hut Liquid Cultures   PhytoExtractum Kratom Powder for Sale   MagicBag.co All-In-One Bags That Don't Suck


Similar ThreadsPosterViewsRepliesLast post
* Agar, should spawn this? (Pics) Alger 1,379 4 12/20/02 03:19 PM
by Bavet
* Problems getting Rhizo growth from MEA
( 1 2 all )
upupup 5,403 32 12/05/02 06:44 AM
by stonErollEr1
* Agar Strain Isolation - Is it worth it?
( 1 2 all )
Blue Helix 13,138 20 02/23/05 01:07 AM
by Blue Helix
* Cloning from dried mushroom tissue techniques?
( 1 2 all )
SkysTheLimit 8,157 25 03/29/22 04:33 PM
by sedulous
* agar transfers
( 1 2 all )
thisone 3,398 22 11/24/03 01:33 PM
by sinoptiK
* Re: I found out I'm getting an agar set for xmas! Anonymous 1,862 10 12/22/99 05:22 PM
by AnubisRonin
* Opions Please "Liquid Growth Medium Q/A" DrShroomAholic 2,694 10 07/10/03 10:22 PM
by micro
* Methods of storeing a sterile tissue sample XxMuSHRooMHeaDxX 3,433 19 12/24/01 02:53 AM
by maxxjones

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, bodhisatta
257 topic views. 0 members, 3 guests and 3 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.007 seconds on 14 queries.