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OfflineEclipse3130
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Psilocin as a degradation byproduct of Psilocybin
    #27713906 - 03/30/22 11:00 AM (2 years, 1 month ago)

Curious on your guy's thoughts on this. I was chatting with the lab that designed Psilocybin potency tests along with many others, Miraculix-Lab out of Germany, Felix Blei CEO and Founder, pharmaceutical microbiologist.

What was previously thought Psilocybin and Psilocin were produced in tandem side by side along with other minor alkaloids and beta-carbolines in Psilocybe species, as well as the blueing reaction was thought to be an indication of Psilocin potency - seems not to be the case.

The claim is this:

Psilocin is a DEGRADATION BYPRODUCT of Psilocybin.



Meaning, 2 enzymes found in fungi de-phosphorylase and laccase are responsible for breaking down Psilocybin into Psilocin and then into its blue pigmentation byproducts when water is present.

PsiK(4-Hydroxytryptamine kinase) has a high affinity for Psilocin and readily rephosphorylates any Psilocin back into Psilocybin when the fruit is growing.

Blueing isn't actually a result of Psilocin potency, rather it is the result of Psilocybin dephosphorylation and degradation into Psilocin and its pigmented degradation byproducts via enzymes that are present within the fruiting bodies.

Lab test results that show Psilocin percentages is actually degraded psilocybin in the process of breaking down.

Apparently when a fruit is immediately freeze dried and tested, very little to no psilocin is present, the process of dehydrating(mainly the time elapsed that water is still present in the fruit) allows the enzymes to go to work and help cleave the phosphate group of Psilocybin and convert into Psilocin and its degradation pigments. The storage thereafter is also important, shown light exposure, extremely cold and hot temperatures degrade Psilocybin the quickest, while room temperature void of light preserves it the longest.

Fresh fruits are more potent not only because of a higher Psilocybin content, but also because a larger content of the traces of minor alkaloids and beta-carbolines are still present as well.



(Homogenized powder of P. Cubensis stored in zip lock baggy i.e not void of oxygen and cell walls completely broken into powder provide greatest rate of degradation)




https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7004109/


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Edited by Eclipse3130 (03/30/22 11:52 AM)

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InvisibleIcyurmt
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Re: Psilocin as a degradation byproduct of Psilocybin [Re: Eclipse3130]
    #27713922 - 03/30/22 11:23 AM (2 years, 1 month ago)

This is the paper that's coming from if anyone else is interested.
Injury‐Triggered Blueing Reactions of Psilocybe “Magic” Mushrooms

The laccase enzymes apparently link the oxidized psilocin together into chains forming oligomers/polymers which are responsible for the blue color. There's also apparently a kinase enzyme they identified that is not only responsible for the original phosphotransfer during psilocybins biosyntheses but can also re-phosphorylate psilocin back into psilocybin, if I'm understanding it correctly. Fascinating stuff imo

Edit: wrote this that before I saw your edit.


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Edited by Icyurmt (03/30/22 11:26 AM)

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OfflineEclipse3130
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Re: Psilocin as a degradation byproduct of Psilocybin [Re: Icyurmt] * 1
    #27713927 - 03/30/22 11:26 AM (2 years, 1 month ago)

It's incredible information. Big perspective change

Thanks for that link, I will add it to the bottom of the post as well.


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"In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
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Perceives Itself"

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OfflineEclipse3130
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Re: Psilocin as a degradation byproduct of Psilocybin [Re: Icyurmt] * 1
    #27713936 - 03/30/22 11:34 AM (2 years, 1 month ago)

As we also previously understood Psilocin does not fare well as a stable alkaloid, it degrades much more rapidly.

I guess the conclusions of this would be if using a dehydrator the goal should be to harvest and dehydrate as quickly as possible to prevent further degradation, and store outside of light and air. It seems avoiding the inevitable percentage break down of psilocybin during dehydration is impossible but it can be minimized.

As well as blueing isn't necessarily correlated to potency, it is correlated to enzyme content and probably chitin structure - the density of the fruit body and the amount of enzymes present could play a role in reducing degradation of psilocybin.

The real question I have is how long can Psilocin be preserved trapped within a dried fruit body stored in an air tight container void of light?


--------------------
"In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
Perceives Itself"

Edited by Eclipse3130 (03/30/22 11:44 AM)

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InvisibleQM33
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Re: Psilocin as a degradation byproduct of Psilocybin [Re: Eclipse3130]
    #27713965 - 03/30/22 11:59 AM (2 years, 1 month ago)

:happyclaps:

Wow. Epic. Beautiful.

Thank you. <3


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