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OfflineSeamonkey84
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Seamonkey84’s dive into the myco-verse
    #27710934 - 03/27/22 08:15 PM (2 years, 9 months ago)

Newbie myco nut here. I feel like mycology is a next step for me as a biology nut. I’ve kept exotic fish and animals my whole life, I have an indoor garden in a legal state, and I’ve made wine and mead. This seems to combine skills from all of those and takes them a step further with the need for sanitization/sterilization. Honestly the idea of having a mini bio lab setup just makes me giddy inside. So After several weeks of reading, youtubeing, and planning, I finally inoculated my first bag of grains today!
I don’t have a pressure canner yet, so I bought a couple pre sterilized injectable grain bags from a local company (they’re actually a sponsor here!). For a flow hood, I put together a vertical chamber using a few plastic totes, a spare blower fan, and a HEPA filter from a air purifier. Honestly I’m really happy with the way this all fit together. I ordered the speed controller for the blower fan, and I might add some LEDs into it for work lights.




I cut the access window large enough to allow qt jars to have plenty of clearance.


I got a 4 cultured plates of a few isolates instead of spores. I decided to go with the Z-strain for my first grow.


Here I have everything prepped and sprayed down with rubbing alcohol, the blower was turned on to purge the box well before putting the items in to dry. I’m using bottles of reduced sugar apple juice as a LC solution. I figured I’d treat it like yeast since LC recipes are basically the same as yeast starters. To reduce the handling of my master culture, I took out what I needed all at once (roughly 1/8-1/4 of the plate) I performed a mycelium transfer to a blank agar plate, some into a bottle of apple juice to start a LC, and the rest got injected  into a 3lb grain bag.



Fingers crossed that the filter works and I avoided contamination.
When I’m all done, the flow hood collapses into itself for storage :cool:


I got my LC and inoculated grain bag sitting on top of my plant grow tent. I’ll continue to update as I see progress or problems.

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InvisibleAtomsplit
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27710947 - 03/27/22 08:23 PM (2 years, 9 months ago)

Welcome! I like unorthodox approaches to anything but I think a SAB will work better than your really cool build.

But I don't see how clearing the box with filtered air can hurt. I just don't think it's a necessary step.

We can learn together.

:popcorn:


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.

Edited by Atomsplit (03/27/22 08:35 PM)

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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: Atomsplit]
    #27710950 - 03/27/22 08:37 PM (2 years, 9 months ago)

I’ve seen many videos where the person is using a vertical flow hood instead of a horizontal laminar flow. I figured Its basically a mini post it I’ve pressure chamber. I actually saw this video after I put mine together

Edited by Seamonkey84 (03/27/22 08:39 PM)

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InvisibleAtomsplit
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27710963 - 03/27/22 08:54 PM (2 years, 9 months ago)



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OfflineWeavieWonder
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27710966 - 03/27/22 08:58 PM (2 years, 9 months ago)

Not to be hater, but if that thing works I will eat my own shoes.

:whoawut:

Let us know how it goes.

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OfflineMycCheck
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27710975 - 03/27/22 09:09 PM (2 years, 9 months ago)

Im 12 shoeboxes and one monotub into the game.  The Zstrain was the most beginner friendly variety out of the 4 I raised

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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: WeavieWonder]
    #27710992 - 03/27/22 09:31 PM (2 years, 9 months ago)

Fingers crossed, but we’ll know in a few days if I have contamination on the agars. Though I’m really wondering if the high rate of air flow makes the filter less efficient. I can’t keep a lighter lit in front of the opening.

Edited by Seamonkey84 (03/27/22 09:33 PM)

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InvisibleLotKid
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84] * 2
    #27711165 - 03/28/22 02:44 AM (2 years, 9 months ago)

:popcorn:


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OfflineBobbins
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Re: Seamonkey84’s dive into the myco-verse [Re: LotKid]
    #27711174 - 03/28/22 03:13 AM (2 years, 9 months ago)

Another day another disruptive innovation. It makes you wonder how no-one ever came up with this before.


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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: Bobbins]
    #27711263 - 03/28/22 07:45 AM (2 years, 9 months ago)

Well it’s not proven yet, and just reading in another post that positive pressure boxes keep circulating some of the contamination around instead of it all being blown out. If this doesn’t work, I can still use the filter part or up size it to another filter if that’s the case

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InvisibleMysticMycologist
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Re: Seamonkey84’s dive into the myco-verse [Re: Bobbins]
    #27711307 - 03/28/22 08:36 AM (2 years, 9 months ago)

Could it be that because without laminar flow, a good SAB will out preform these contraptions? By all means, keep innovating, but I just have a feeling that my big ol’ SAB works as good or better.


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Offlinesan pedro guy
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Re: Seamonkey84’s dive into the myco-verse [Re: MysticMycologist] * 2
    #27711337 - 03/28/22 09:24 AM (2 years, 9 months ago)

turbulent air flow is frowned upon around here.

Still Air boxes work really well and so simple it’s hard to justify anything more for small scale stuff.

Wishing you no or very low contams!


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Noob Grow Along 2022

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InvisibleMysticMycologist
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Re: Seamonkey84’s dive into the myco-verse [Re: san pedro guy] * 2
    #27711361 - 03/28/22 09:54 AM (2 years, 9 months ago)



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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: MysticMycologist]
    #27711383 - 03/28/22 10:19 AM (2 years, 9 months ago)

Yea I read that one this morning, if this doesn’t work I might just use that too box eight he filter as a horizontal flow hood, or make it bigger if that’s the case. Or just stick with a SAB

Edited by Seamonkey84 (03/28/22 10:20 AM)

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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27713724 - 03/30/22 07:16 AM (2 years, 9 months ago)

Well I checked yesterday afternoon (48hrs after the transfer) and the agars did not show any signs of contamination.  there’s signs of mycelium growth on the new plate and the bare spots from where I scraped the original plate. I’ll check again today when I get home and try to take a pic.

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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27714283 - 03/30/22 03:49 PM (2 years, 9 months ago)

72hr update on the agar plate. I see no contamination growing, just the transferee mycelium. I really can’t seem to get a clear pic since there’s condensation and glare on the cover. Also, the guy who I got the blank plate from used some sort of pink coloring that didn’t dissolve all the way so it looks like there’s does forming but they’re not on the agar surface. I’m not seeing any contamination on the original colonized plate either, just new growth on the areas I scrapped off.

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Offlinesan pedro guy
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27714509 - 03/30/22 06:50 PM (2 years, 9 months ago)

hell yea, looking good brother!


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OfflineWeavieWonder
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84] * 1
    #27714514 - 03/30/22 06:54 PM (2 years, 9 months ago)

:popcorn:

Let's have a look at day 7.

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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: WeavieWonder]
    #27716750 - 04/01/22 04:12 PM (2 years, 9 months ago)

So the other night I dropped the agar plate, it landed on one edge. It was sealed and a still intact, but I knew there’s a chance it could of knocked something loose. Well now I see tiny white specks starting to grow along one side, at this point I can’t tell if it’s contamination or some mycelium that got knocked loose from the shock. So for the time being, I’m just going to keep an eye on it and see if it’s the same growth as the culture and merges or if it’s something else and takes over. In the mean time, I got my cordyceps LC in, so I im going to need to really test this with a blank plate before I get a tub of rice sitting in there. Gotta do the expansion for It first anyway.

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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27716803 - 04/01/22 04:46 PM (2 years, 9 months ago)

Oh gawd, more rice :lol:


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InvisibleAshtray161
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27716810 - 04/01/22 04:51 PM (2 years, 9 months ago)

Hey there OP, I like your creativity, but honestly I gotta side with others here and bet on that setup not being as effective as a typical SAB. I wont beat a dead horse on explaining why, *cough laminar flow cough* cuz the folx above me did a very nice job of that, but I'm excited to see how your journey moves along. Most peoples labs look very different on day 100 than day 1 so some growth time is to be expected. I think a lot of people drawn to this craft are the "find out the hard way" types lol including myself.
Good luck and welcome to da shroomery


--------------------
(You Know What Time It Is)
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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: Ashtray161]
    #27717298 - 04/02/22 12:16 AM (2 years, 9 months ago)

Yea learning as I go, still giving this thing a testing though. I prepped the box by spraying down the entire inside with alcohol, then let it run for a hood 10-15 mins. Then I opened a blank agar plate and set it face up, while I wrapped 4 other agar plates with parafilm. I took my time with the wrapping, so that plate had several minutes sitting open. It’s been labeled and set aside it watch for growth.
Then after that, I put two different cordyceps LC syringes to agar and expansion. I didn’t use the whole syringe of either though, so I still have clean backups.

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InvisibleMysticMycologist
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27717328 - 04/02/22 12:50 AM (2 years, 9 months ago)

I try make sure to give plates 10-14 days before I am sure they are contam free. Especially when trying a new sterile technique process or using unsterilized condiment cups. Sometimes the nasties can take a while to show up.


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OfflineLilJonB
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Re: Seamonkey84’s dive into the myco-verse [Re: MysticMycologist]
    #27717602 - 04/02/22 08:02 AM (2 years, 9 months ago)

What's up bro!

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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: LilJonB]
    #27717604 - 04/02/22 08:12 AM (2 years, 9 months ago)

Hey hey! Fancy seeing you here lol.

Well mycelium from the transfer has met one of the specks that are growing. Going to keep eye on it and see what happens. Part of what fascinates me with this microbiology stuff.

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InvisibleMysticMycologist
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27717639 - 04/02/22 08:57 AM (2 years, 9 months ago)

Those satellites look a lot like trich.


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Two eyes to look, One eye to see.
Prying open my third eye

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OfflineBobbins
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Re: Seamonkey84’s dive into the myco-verse [Re: MysticMycologist]
    #27717689 - 04/02/22 09:48 AM (2 years, 9 months ago)

Maybe your blow hood ain't working so well.


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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: MysticMycologist]
    #27718904 - 04/03/22 12:38 AM (2 years, 9 months ago)

Yea those spots turned green 😐. Goin to work with it as an SAB for now until I see the results from the open plate test. Since eveyone says SAB work, I’ll practice with it. I also got filter disks and injection ports, so going forward I can also go no touch with just about everything up till mixing to bulk. No pour agar, LC, and grain jars and bags all with modified lids/injection ports.

But still playing with the simple blower filter build. The mycology supply site sells a small FFU that uses a readily available 13x15 HEPA filter (AP1518). I don’t know what that filters resistance is, it “only” needs 135cfm of air coming out of it to be 100fps. Would something like a 442cfm blower with 550Pa (ipower 6”) be able to handle it, or even need to be turned down?  I also don’t know the output curve of the blower either.

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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27719079 - 04/03/22 08:01 AM (2 years, 9 months ago)

If the open plate test is a fail, I think I still have a workable flow hood. It would be small, but I can at the least use the top bin with the blower and filter as a mini flow hood for agar work or cooling a small pot of rice for cordyceps. I got the controller for the fan so I can adjust the output. Might have to buy a new tote and put the hole on one side so it’s not a pain to balance or mount on its side.


But fingers crossed it didn’t completely screw up the mycelium water/slurry I used to inoculate my grain bag and LC. The grain bag is coming along, I noticed a bit of growth on the bottom, and I picked it up the bag to see this. Sorry for the horrible lighting



Edited by Seamonkey84 (04/03/22 08:10 AM)

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InvisibleAshtray161
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84] * 2
    #27719662 - 04/03/22 04:17 PM (2 years, 9 months ago)

Friend you're missing a HUGE part of a flowhood, the flow. Laminar flow to be exact. Blowing turbulent air throu a filter does absoutlely nothing, it just picks up contams from the dirty air around it. You need laminar flow to make a flowhod, which is why they're so expensive


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InvisibleMysticMycologist
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Re: Seamonkey84’s dive into the myco-verse [Re: Ashtray161]
    #27719693 - 04/03/22 04:38 PM (2 years, 9 months ago)

:whathesaid:
Yup.
SAB bro. They work if you work them.


--------------------
Two eyes to look, One eye to see.
Prying open my third eye

Edited by MysticMycologist (04/03/22 05:36 PM)

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InvisibleAshtray161
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Re: Seamonkey84’s dive into the myco-verse [Re: MysticMycologist] * 1
    #27719717 - 04/03/22 04:47 PM (2 years, 9 months ago)

Yep SABs work very well. Using gravity and still air to keep your work clean.

If it was as easy as sticking a fan behind a filter box we wouldnt spend thousands on flowhoods lol


--------------------
(You Know What Time It Is)
Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086:elmo:
"You never have to prove the fool a fool, just let them speak."
Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED.
Be Gay, Do Crime 161 1312

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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: Ashtray161]
    #27720433 - 04/04/22 05:13 AM (2 years, 9 months ago)

What’s the difference in having a furnace blower pushing air into a box behind a filter? None of the designs I’ve seen have any thing to make the air flow laminar before going through the filter. What I’ve read says the back pressure going through filter itself at a certain speed is what causes the air to line up and be laminar.

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OfflineKROM
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27720460 - 04/04/22 05:45 AM (2 years, 9 months ago)



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InvisibleSunny Skies
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Re: Seamonkey84’s dive into the myco-verse [Re: KROM]
    #27720503 - 04/04/22 07:11 AM (2 years, 9 months ago)

Seamonkey I would do a bit more reading up on flow hoods...honestly you dont need one to get great results..its all in your sterile methods..keeping it simple works.

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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: KROM]
    #27720506 - 04/04/22 07:13 AM (2 years, 9 months ago)

That’s just talking about how to choose the blower to match the filter so it has enough pressure and volume to do the job. So long as it’s pushing air through fast enough to get at least 100fpm. The blower I have for the little filter would need to be turned down to not instantly blow out the flame to a lighter when I did the lighter test. I understand that I probably shouldn’t have the blower facing directly at the filter, just to get the space pressurized evenly.

I understand that I don’t need to have a hood, just makes things so much easier and less risky. But yea, I know there’s ways around it and just need to practice with a SAB. Putting injection ports on everything including no pour agar lids would actually really simply things even with a flow hood. Cloning and mycelium transfers can be done with needle biopsy size samples. The biggest thing is I didn’t want to grow cordyceps just in jars, but leaving a tub of rice to cool and dry in a SAB just doesn’t seem right

Edited by Seamonkey84 (04/04/22 07:27 AM)

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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84] * 1
    #27726342 - 04/08/22 09:44 PM (2 years, 9 months ago)

Here is the test plate after one week. It was left open in the box while I was wrapping 4 other plates, then I wrapped it as the 5th plate. Looks like it works!


3 days after break and shake on my first batch, this z-strain (or starting from pieces off a cultured plate) seems to be colonizing this 3lb bag faster than my buddy’s 8oz rice bags that he started with spores syringe. Now I gotta find some coco. Prefer to get it locally but that’s tough right now. The pet store I work at has been out for about a month.


The two cordyceps plates I started to keep a culture (and test the LC syringe). Now I have to test the LC I made. I have all the ingredients for the nutrient broth. Im getting a small rice cooker just for this, don’t want to add off flavors to the household one.



Some hornworms that’s Im going to attempt to grow them in



Got 4 packets of Agar from the Asian market. Prob going to do some no pour tek with 4oz jars and modified lids. The Josex poke tek combined with injection ports seem a lot easier than setting up the box every time I want to do anything.

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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27726903 - 04/09/22 08:30 AM (2 years, 9 months ago)

do you clone the bug eating cordyceps on regular pda or lme agar? do they only need the bugs to "fruit"? this is so brutal

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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: dyel]
    #27726966 - 04/09/22 09:12 AM (2 years, 9 months ago)

Cordyceps  Militaris will fruit from a nutrient broth enriched rice, that’s how it’s commercially grown. But in nature, it also have a wide range of butterfly/moth/beetle species it will parasitize.  The sinsensis species needs a host to fruit, but their natural host is only a mountain moth larva. there’s some success with fruiting them from silk worms. I don’t have live silk worms at the moment, so I’m trying packaged/canned silk worms sold for lizard food and I have live hornworms. Sinensis is found growing from the larval stage, while Militaris unusually comes out of the pupa. Im almost positive Militaris will work, just waiting for a few of these to pupate. With the sinensis, I’m going to have to try injecting them prob midway through their growth stage.
Im not sure if Militaris is found in my state, but it’s likely. Im going to do some looking while I’m out either way.

Edited by Seamonkey84 (04/09/22 09:17 AM)

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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27735322 - 04/15/22 12:15 AM (2 years, 8 months ago)

My Apple juice LC of Z-strain and two bottles of cordyceps Militaris seem to be testing clean on agar (sampled and streaked with inoculation loop)



The Cordy Sinensis developed a nice pellicle while in the Apple juice


My grain bag is fully colonized and ready to go


Boil in bag sanitized some CVG


The tissue samples I took from a gold oyster I bought from Whole Foods aren’t so clean. There’s one I have hopes of some mycelium growth, just hope I can find a clean spot to transfer. I should crack that open and remove that one that’s just rotting and flush it with some hydrogen peroxide.

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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27735355 - 04/15/22 01:12 AM (2 years, 8 months ago)

Looks like That Z-strain plate has trich all over it to me. It may turn green soon.


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Re: Seamonkey84’s dive into the myco-verse [Re: MysticMycologist]
    #27735376 - 04/15/22 02:45 AM (2 years, 8 months ago)

Bruh are you sure your grain bag is OK, seems a lot of moisture in that bag... Could be bacterial?


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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: Bobbins]
    #27735465 - 04/15/22 06:17 AM (2 years, 8 months ago)

The grains have pulled away from the bag and a lot of condensation is forming…

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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84] * 3
    #27735573 - 04/15/22 08:25 AM (2 years, 8 months ago)

Looks bacterial


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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: LotKid]
    #27739750 - 04/18/22 06:28 AM (2 years, 8 months ago)

Well the plates are clean, all growth have remained white and consistent with each other. When I was breaking up the grain bag, I smelled nothing but fresh mushrooms. I made three shoeboxes, mixed 1:1 with CVG, and I also got two boxes of cordyceps started.

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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27743738 - 04/20/22 02:20 PM (2 years, 8 months ago)

I’m starting to hate parafilm, or I have to get a new roll (got this one on amazon). Or maybe I should fold a wider piece so it’s doubled all the way around. I’ve had several plates that were doing perfectly until suddenly there’s contamination on the very edge of the plate, they line right up with tiny holes in the film that weren’t there before.
I know I should prob put the plates into ziplock bags too.

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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27748961 - 04/23/22 11:41 PM (2 years, 8 months ago)

Day six update on the shoeboxes, all three are coming along nicely



Got some jars for agar, LC, and grains modified. A few for growing cordyceps in the jars. I had to toss one of the cordyceps Militaris bins due to two patches of mold that started to grow.

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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27752529 - 04/26/22 06:13 PM (2 years, 8 months ago)

Day 9 on the shoeboxes. Here’s the same one I posted above, the one that’s been the fastest to colonize. I put this box into fruiting conditions the other day, and the colonization on the side with the cracked open lid has just exploded



The cordyceps Militaris bin that didn’t get contaminated is fully colonized (was prob ready two days ago) and I stuck it into a modified tote to start fruiting. The temps in the basement are much better for this species, so the tote is really to keep the contamination out.


No updates pics of the cordyceps sinensis project, but the pasteurized  silk worms are about 75% colonized.  The live  hornworm that I can see through the side of the container looks like it’s now likely dead. It might be colonizing from the inside out as I see some areas of white protruding along parts of the body. If they turn all fuzzy or different colors I’m just tossing the whole container.

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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27752636 - 04/26/22 08:03 PM (2 years, 8 months ago)

Are you trying to do unmodified shoeboxes with those?

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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: GhostHustler]
    #27752691 - 04/26/22 09:24 PM (2 years, 8 months ago)

Yes I am. I realized after that I prob should of filled the box up more.

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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27756563 - 04/29/22 01:13 PM (2 years, 8 months ago)

The pasteurized silkworms are still being colonized by cordyceps sinensis. Once they’re fully colonized I’m going to stick them in the fridge to simulate overwintering.  apparently they grow slowly but start to fruit in the cold conditions, 15-20 weeks to fruit in in the cold.


The hornworms that were inoculated and buried themselves are showing signs of being colonized from the inside out, there are two against the side of the container, one more visible than the other.



The Cordyceps militaris rice bin that didn’t show any mold is changing color, looks good to me,


I have several hornworm pupas that I am going to store in the fridge to keep dormant, along with a few more hornworms that will turn in the next week or so. I’m starting the next batch of LC soon to inoculate them all at once.


The couple drops of the morel LC I put on agar produced one point of growth, wasn’t sure what to make of it since it was bullseye patterned, but it’s fully colonized in no time and the entire plate is a dark brownish to almost black.
This was 4 days ago

Now



The shoebox I put into “growing conditions” is definitely colonizing faster than the others, but I’m still hesitant to crack the lids on the other two until I know I’m not just making fungus gnat breeding beds.



One that still has the lid latched, if you could see past the condensation, the surface isn’t nearly as colonized



After I make up my jars of LC solution, I’m going to start them with Mazatat (mazatapec x TAT), cordyceps militaris, and bulk cultures of Morchella rufobrunnea to apply outdoors.

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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84] * 1
    #27756906 - 04/29/22 06:31 PM (2 years, 8 months ago)

:neat:


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Re: Seamonkey84’s dive into the myco-verse [Re: LotKid]
    #27757004 - 04/29/22 08:21 PM (2 years, 8 months ago)

One of the shoebox started pinning! Turns out this shoebox has a warped rim so the lid doesn’t close down on one of the long sides.  It’s been getting FAE this whole time 😝

Edited by Seamonkey84 (04/30/22 03:32 PM)

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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27761137 - 05/03/22 09:01 AM (2 years, 8 months ago)

White spots on the young caps, from water droplets or signs of stress? Anything to be concerned about?


This shoebox was the last one I filled and the one with the least substrate in it. It has the warped rim so the lid didn’t close down on it all the way and has had FAE the whole time.  I know that is fine since there’s still plenty of condensation. But it’s not colonizing evenly, but I’m not seeing signs of contamination on the surface, just pins popping through the CVG in the spots that’s not fully colonized.


I didn’t take any pics of the other two boxes, but they’re fully colonized on the surface. One I intentionally cracked the lid on is pinning, and  the final box has been cracked open too.

The Cordyceps militaris bin looks like a very thick sauced Mac n cheese. Not much to see on the sinensis


I started LC of morels and militaris, playing with new stir plate. I made a 4% honey water solution and added peptone. Steam sterilized for 2hrs. Still want to get some LME for LC and agar. Oh, still need to make my no pour agar, going with potato honey peptone agar for now. I gotta get to the brew shop, it’s tricky between my work schedule and their shortened hours.
Talking to my guy, I think I’ll be adding the Starry/Nebula APE to my collection…

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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84] * 1
    #27765076 - 05/05/22 08:21 PM (2 years, 8 months ago)

First caps are opened! These are cubes right :tongue: ?



Second box

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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27765187 - 05/05/22 09:54 PM (2 years, 8 months ago)

They appear to be. Congrats


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Re: Seamonkey84’s dive into the myco-verse [Re: MysticMycologist] * 2
    #27769028 - 05/08/22 06:40 PM (2 years, 8 months ago)

Second box



Both blocks have been soaked with distilled water for a day and are sitting with the lid sitting back on top.

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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84] * 1
    #27769063 - 05/08/22 07:13 PM (2 years, 8 months ago)

Very good seamonkey!
:rockon:


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