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InvisibleMysticMycologist
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27735355 - 04/15/22 01:12 AM (2 years, 8 months ago)

Looks like That Z-strain plate has trich all over it to me. It may turn green soon.


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OfflineBobbins
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Re: Seamonkey84’s dive into the myco-verse [Re: MysticMycologist]
    #27735376 - 04/15/22 02:45 AM (2 years, 8 months ago)

Bruh are you sure your grain bag is OK, seems a lot of moisture in that bag... Could be bacterial?


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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: Bobbins]
    #27735465 - 04/15/22 06:17 AM (2 years, 8 months ago)

The grains have pulled away from the bag and a lot of condensation is forming…

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InvisibleLotKid
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84] * 3
    #27735573 - 04/15/22 08:25 AM (2 years, 8 months ago)

Looks bacterial


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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: LotKid]
    #27739750 - 04/18/22 06:28 AM (2 years, 8 months ago)

Well the plates are clean, all growth have remained white and consistent with each other. When I was breaking up the grain bag, I smelled nothing but fresh mushrooms. I made three shoeboxes, mixed 1:1 with CVG, and I also got two boxes of cordyceps started.

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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27743738 - 04/20/22 02:20 PM (2 years, 8 months ago)

I’m starting to hate parafilm, or I have to get a new roll (got this one on amazon). Or maybe I should fold a wider piece so it’s doubled all the way around. I’ve had several plates that were doing perfectly until suddenly there’s contamination on the very edge of the plate, they line right up with tiny holes in the film that weren’t there before.
I know I should prob put the plates into ziplock bags too.

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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27748961 - 04/23/22 11:41 PM (2 years, 8 months ago)

Day six update on the shoeboxes, all three are coming along nicely



Got some jars for agar, LC, and grains modified. A few for growing cordyceps in the jars. I had to toss one of the cordyceps Militaris bins due to two patches of mold that started to grow.

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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27752529 - 04/26/22 06:13 PM (2 years, 8 months ago)

Day 9 on the shoeboxes. Here’s the same one I posted above, the one that’s been the fastest to colonize. I put this box into fruiting conditions the other day, and the colonization on the side with the cracked open lid has just exploded



The cordyceps Militaris bin that didn’t get contaminated is fully colonized (was prob ready two days ago) and I stuck it into a modified tote to start fruiting. The temps in the basement are much better for this species, so the tote is really to keep the contamination out.


No updates pics of the cordyceps sinensis project, but the pasteurized  silk worms are about 75% colonized.  The live  hornworm that I can see through the side of the container looks like it’s now likely dead. It might be colonizing from the inside out as I see some areas of white protruding along parts of the body. If they turn all fuzzy or different colors I’m just tossing the whole container.

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OfflineGhostHustler
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27752636 - 04/26/22 08:03 PM (2 years, 8 months ago)

Are you trying to do unmodified shoeboxes with those?

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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: GhostHustler]
    #27752691 - 04/26/22 09:24 PM (2 years, 8 months ago)

Yes I am. I realized after that I prob should of filled the box up more.

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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27756563 - 04/29/22 01:13 PM (2 years, 8 months ago)

The pasteurized silkworms are still being colonized by cordyceps sinensis. Once they’re fully colonized I’m going to stick them in the fridge to simulate overwintering.  apparently they grow slowly but start to fruit in the cold conditions, 15-20 weeks to fruit in in the cold.


The hornworms that were inoculated and buried themselves are showing signs of being colonized from the inside out, there are two against the side of the container, one more visible than the other.



The Cordyceps militaris rice bin that didn’t show any mold is changing color, looks good to me,


I have several hornworm pupas that I am going to store in the fridge to keep dormant, along with a few more hornworms that will turn in the next week or so. I’m starting the next batch of LC soon to inoculate them all at once.


The couple drops of the morel LC I put on agar produced one point of growth, wasn’t sure what to make of it since it was bullseye patterned, but it’s fully colonized in no time and the entire plate is a dark brownish to almost black.
This was 4 days ago

Now



The shoebox I put into “growing conditions” is definitely colonizing faster than the others, but I’m still hesitant to crack the lids on the other two until I know I’m not just making fungus gnat breeding beds.



One that still has the lid latched, if you could see past the condensation, the surface isn’t nearly as colonized



After I make up my jars of LC solution, I’m going to start them with Mazatat (mazatapec x TAT), cordyceps militaris, and bulk cultures of Morchella rufobrunnea to apply outdoors.

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InvisibleLotKid
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84] * 1
    #27756906 - 04/29/22 06:31 PM (2 years, 8 months ago)

:neat:


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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: LotKid]
    #27757004 - 04/29/22 08:21 PM (2 years, 8 months ago)

One of the shoebox started pinning! Turns out this shoebox has a warped rim so the lid doesn’t close down on one of the long sides.  It’s been getting FAE this whole time 😝

Edited by Seamonkey84 (04/30/22 03:32 PM)

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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27761137 - 05/03/22 09:01 AM (2 years, 8 months ago)

White spots on the young caps, from water droplets or signs of stress? Anything to be concerned about?


This shoebox was the last one I filled and the one with the least substrate in it. It has the warped rim so the lid didn’t close down on it all the way and has had FAE the whole time.  I know that is fine since there’s still plenty of condensation. But it’s not colonizing evenly, but I’m not seeing signs of contamination on the surface, just pins popping through the CVG in the spots that’s not fully colonized.


I didn’t take any pics of the other two boxes, but they’re fully colonized on the surface. One I intentionally cracked the lid on is pinning, and  the final box has been cracked open too.

The Cordyceps militaris bin looks like a very thick sauced Mac n cheese. Not much to see on the sinensis


I started LC of morels and militaris, playing with new stir plate. I made a 4% honey water solution and added peptone. Steam sterilized for 2hrs. Still want to get some LME for LC and agar. Oh, still need to make my no pour agar, going with potato honey peptone agar for now. I gotta get to the brew shop, it’s tricky between my work schedule and their shortened hours.
Talking to my guy, I think I’ll be adding the Starry/Nebula APE to my collection…

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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84] * 1
    #27765076 - 05/05/22 08:21 PM (2 years, 8 months ago)

First caps are opened! These are cubes right :tongue: ?



Second box

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InvisibleMysticMycologist
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
    #27765187 - 05/05/22 09:54 PM (2 years, 8 months ago)

They appear to be. Congrats


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OfflineSeamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: MysticMycologist] * 2
    #27769028 - 05/08/22 06:40 PM (2 years, 8 months ago)

Second box



Both blocks have been soaked with distilled water for a day and are sitting with the lid sitting back on top.

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InvisibleMysticMycologist
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84] * 1
    #27769063 - 05/08/22 07:13 PM (2 years, 8 months ago)

Very good seamonkey!
:rockon:


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