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Ashtray161
SettledNomad



Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
#27716810 - 04/01/22 04:51 PM (2 years, 9 months ago) |
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Hey there OP, I like your creativity, but honestly I gotta side with others here and bet on that setup not being as effective as a typical SAB. I wont beat a dead horse on explaining why, *cough laminar flow cough* cuz the folx above me did a very nice job of that, but I'm excited to see how your journey moves along. Most peoples labs look very different on day 100 than day 1 so some growth time is to be expected. I think a lot of people drawn to this craft are the "find out the hard way" types lol including myself. Good luck and welcome to da shroomery
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(You Know What Time It Is)
Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086
"You never have to prove the fool a fool, just let them speak."
Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED.
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Seamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: Ashtray161]
#27717298 - 04/02/22 12:16 AM (2 years, 9 months ago) |
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Yea learning as I go, still giving this thing a testing though. I prepped the box by spraying down the entire inside with alcohol, then let it run for a hood 10-15 mins. Then I opened a blank agar plate and set it face up, while I wrapped 4 other agar plates with parafilm. I took my time with the wrapping, so that plate had several minutes sitting open. It’s been labeled and set aside it watch for growth. Then after that, I put two different cordyceps LC syringes to agar and expansion. I didn’t use the whole syringe of either though, so I still have clean backups.
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MysticMycologist
Dirt Sherpa



Registered: 10/14/21
Posts: 1,755
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
#27717328 - 04/02/22 12:50 AM (2 years, 9 months ago) |
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I try make sure to give plates 10-14 days before I am sure they are contam free. Especially when trying a new sterile technique process or using unsterilized condiment cups. Sometimes the nasties can take a while to show up.
-------------------- Two eyes to look, One eye to see.
Prying open my third eye

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LilJonB
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What's up bro!
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Seamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: LilJonB]
#27717604 - 04/02/22 08:12 AM (2 years, 9 months ago) |
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Hey hey! Fancy seeing you here lol.
Well mycelium from the transfer has met one of the specks that are growing. Going to keep eye on it and see what happens. Part of what fascinates me with this microbiology stuff.
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MysticMycologist
Dirt Sherpa



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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
#27717639 - 04/02/22 08:57 AM (2 years, 9 months ago) |
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Those satellites look a lot like trich.
-------------------- Two eyes to look, One eye to see.
Prying open my third eye

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Bobbins
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Maybe your blow hood ain't working so well.
-------------------- DeALeRsHrOoMs
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Seamonkey84
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Yea those spots turned green 😐. Goin to work with it as an SAB for now until I see the results from the open plate test. Since eveyone says SAB work, I’ll practice with it. I also got filter disks and injection ports, so going forward I can also go no touch with just about everything up till mixing to bulk. No pour agar, LC, and grain jars and bags all with modified lids/injection ports.
But still playing with the simple blower filter build. The mycology supply site sells a small FFU that uses a readily available 13x15 HEPA filter (AP1518). I don’t know what that filters resistance is, it “only” needs 135cfm of air coming out of it to be 100fps. Would something like a 442cfm blower with 550Pa (ipower 6”) be able to handle it, or even need to be turned down? I also don’t know the output curve of the blower either.
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Seamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
#27719079 - 04/03/22 08:01 AM (2 years, 9 months ago) |
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If the open plate test is a fail, I think I still have a workable flow hood. It would be small, but I can at the least use the top bin with the blower and filter as a mini flow hood for agar work or cooling a small pot of rice for cordyceps. I got the controller for the fan so I can adjust the output. Might have to buy a new tote and put the hole on one side so it’s not a pain to balance or mount on its side.

But fingers crossed it didn’t completely screw up the mycelium water/slurry I used to inoculate my grain bag and LC. The grain bag is coming along, I noticed a bit of growth on the bottom, and I picked it up the bag to see this. Sorry for the horrible lighting

Edited by Seamonkey84 (04/03/22 08:10 AM)
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Ashtray161
SettledNomad



Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84] 2
#27719662 - 04/03/22 04:17 PM (2 years, 9 months ago) |
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Friend you're missing a HUGE part of a flowhood, the flow. Laminar flow to be exact. Blowing turbulent air throu a filter does absoutlely nothing, it just picks up contams from the dirty air around it. You need laminar flow to make a flowhod, which is why they're so expensive
--------------------
(You Know What Time It Is)
Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086
"You never have to prove the fool a fool, just let them speak."
Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED.
Be Gay, Do Crime 161 1312
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MysticMycologist
Dirt Sherpa



Registered: 10/14/21
Posts: 1,755
Loc: seeking samadhi
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Re: Seamonkey84’s dive into the myco-verse [Re: Ashtray161]
#27719693 - 04/03/22 04:38 PM (2 years, 9 months ago) |
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 Yup. SAB bro. They work if you work them.
-------------------- Two eyes to look, One eye to see.
Prying open my third eye

Edited by MysticMycologist (04/03/22 05:36 PM)
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Ashtray161
SettledNomad



Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
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Yep SABs work very well. Using gravity and still air to keep your work clean.
If it was as easy as sticking a fan behind a filter box we wouldnt spend thousands on flowhoods lol
--------------------
(You Know What Time It Is)
Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086
"You never have to prove the fool a fool, just let them speak."
Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED.
Be Gay, Do Crime 161 1312
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Seamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: Ashtray161]
#27720433 - 04/04/22 05:13 AM (2 years, 9 months ago) |
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What’s the difference in having a furnace blower pushing air into a box behind a filter? None of the designs I’ve seen have any thing to make the air flow laminar before going through the filter. What I’ve read says the back pressure going through filter itself at a certain speed is what causes the air to line up and be laminar.
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KROM
Local Oaf



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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
#27720460 - 04/04/22 05:45 AM (2 years, 9 months ago) |
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🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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Sunny Skies
Cluster Head


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Re: Seamonkey84’s dive into the myco-verse [Re: KROM]
#27720503 - 04/04/22 07:11 AM (2 years, 9 months ago) |
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Seamonkey I would do a bit more reading up on flow hoods...honestly you dont need one to get great results..its all in your sterile methods..keeping it simple works.
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Seamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: KROM]
#27720506 - 04/04/22 07:13 AM (2 years, 9 months ago) |
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That’s just talking about how to choose the blower to match the filter so it has enough pressure and volume to do the job. So long as it’s pushing air through fast enough to get at least 100fpm. The blower I have for the little filter would need to be turned down to not instantly blow out the flame to a lighter when I did the lighter test. I understand that I probably shouldn’t have the blower facing directly at the filter, just to get the space pressurized evenly.
I understand that I don’t need to have a hood, just makes things so much easier and less risky. But yea, I know there’s ways around it and just need to practice with a SAB. Putting injection ports on everything including no pour agar lids would actually really simply things even with a flow hood. Cloning and mycelium transfers can be done with needle biopsy size samples. The biggest thing is I didn’t want to grow cordyceps just in jars, but leaving a tub of rice to cool and dry in a SAB just doesn’t seem right
Edited by Seamonkey84 (04/04/22 07:27 AM)
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Seamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84] 1
#27726342 - 04/08/22 09:44 PM (2 years, 9 months ago) |
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Here is the test plate after one week. It was left open in the box while I was wrapping 4 other plates, then I wrapped it as the 5th plate. Looks like it works!

3 days after break and shake on my first batch, this z-strain (or starting from pieces off a cultured plate) seems to be colonizing this 3lb bag faster than my buddy’s 8oz rice bags that he started with spores syringe. Now I gotta find some coco. Prefer to get it locally but that’s tough right now. The pet store I work at has been out for about a month.

The two cordyceps plates I started to keep a culture (and test the LC syringe). Now I have to test the LC I made. I have all the ingredients for the nutrient broth. Im getting a small rice cooker just for this, don’t want to add off flavors to the household one.


Some hornworms that’s Im going to attempt to grow them in

Got 4 packets of Agar from the Asian market. Prob going to do some no pour tek with 4oz jars and modified lids. The Josex poke tek combined with injection ports seem a lot easier than setting up the box every time I want to do anything.
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dyel



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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
#27726903 - 04/09/22 08:30 AM (2 years, 9 months ago) |
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do you clone the bug eating cordyceps on regular pda or lme agar? do they only need the bugs to "fruit"? this is so brutal
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Seamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: dyel]
#27726966 - 04/09/22 09:12 AM (2 years, 9 months ago) |
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Cordyceps Militaris will fruit from a nutrient broth enriched rice, that’s how it’s commercially grown. But in nature, it also have a wide range of butterfly/moth/beetle species it will parasitize. The sinsensis species needs a host to fruit, but their natural host is only a mountain moth larva. there’s some success with fruiting them from silk worms. I don’t have live silk worms at the moment, so I’m trying packaged/canned silk worms sold for lizard food and I have live hornworms. Sinensis is found growing from the larval stage, while Militaris unusually comes out of the pupa. Im almost positive Militaris will work, just waiting for a few of these to pupate. With the sinensis, I’m going to have to try injecting them prob midway through their growth stage. Im not sure if Militaris is found in my state, but it’s likely. Im going to do some looking while I’m out either way.
Edited by Seamonkey84 (04/09/22 09:17 AM)
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Seamonkey84
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Re: Seamonkey84’s dive into the myco-verse [Re: Seamonkey84]
#27735322 - 04/15/22 12:15 AM (2 years, 8 months ago) |
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My Apple juice LC of Z-strain and two bottles of cordyceps Militaris seem to be testing clean on agar (sampled and streaked with inoculation loop)


The Cordy Sinensis developed a nice pellicle while in the Apple juice

My grain bag is fully colonized and ready to go

Boil in bag sanitized some CVG

The tissue samples I took from a gold oyster I bought from Whole Foods aren’t so clean. There’s one I have hopes of some mycelium growth, just hope I can find a clean spot to transfer. I should crack that open and remove that one that’s just rotting and flush it with some hydrogen peroxide.
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