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OfflineLozMyKeys
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What mould is this? Enmeshed?
    #27702216 - 03/20/22 08:28 AM (1 year, 10 months ago)

My plates started out fine from spores and with each transfer they got worse and worse with this mould. I've seen posts talk about "enmeshed" mould I'm assuming that means its always present within the mycelium so transferring away from it will be impossible.





I can't seem to get away from it. Sometimes its just a halo around the mycelium which then grows over it so you barely can see it. These transfers were the worst yet.

Any help?


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Offlinesan pedro guy
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Re: What mould is this? Enmeshed? [Re: LozMyKeys]
    #27702224 - 03/20/22 08:38 AM (1 year, 10 months ago)

I’m pretty new but I had plates like that, it wasn’t “mould” as far as I could tell.

It was an issue with my agar recipe.


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OfflineLozMyKeys
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Re: What mould is this? Enmeshed? [Re: san pedro guy] * 1
    #27702228 - 03/20/22 09:00 AM (1 year, 10 months ago)

This must be some sort of mould its not allowing the mycelium to grow in certain areas, and others its just completely taken over.


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Offlineshuna
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Re: What mould is this? Enmeshed? [Re: LozMyKeys]
    #27702232 - 03/20/22 09:04 AM (1 year, 10 months ago)

That first pic you might be able to get a transfer, the rest look bad.

Its probably best to start off again from spores on a print imho. You could be at that for months transferring.

Otherwise mybe use a brf verm cake and transfer to that, hoping the myc outruns the bacteria. or search josex poke.


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OfflineLozMyKeys
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Re: What mould is this? Enmeshed? [Re: shuna]
    #27702237 - 03/20/22 09:14 AM (1 year, 10 months ago)

The problem is its underneath the mycelium, so transferring just transfers the mould with it. These were fine from spores to start off no signs of mould them slowly got worse.


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Edited by LozMyKeys (03/20/22 09:14 AM)


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Offlineshuna
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Re: What mould is this? Enmeshed? [Re: LozMyKeys]
    #27702244 - 03/20/22 09:27 AM (1 year, 10 months ago)

Yeah, spent way too much time trying to clean stuff like that up on agar and not knowing better.

Had to go back to a spore print and eventually after like half a dozen times of trying, eventually got some clean myc. Dirty spore prints can be like that.

Mybe someone else will chime in with better ideas.

There is anti bacterial agar and chemicals you can try if its important to keep.

I've tried doing a hot pour, adding another layer of agar on top in the hope that the myc will grow up to the top layer a few hours before the bacteria. Didn't work out for me though.

You could mess about with the agar recipe, low nute content to encourage vigourous myc growth and do a firm agar. Frustrating but you'll get some clean myc one way or another, mybe try a new print.

Edit: Not 100% sterile scalpel or inoculation loop can do this too


Edited by shuna (03/20/22 09:38 AM)


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OfflinePBJ710
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Re: What mould is this? Enmeshed? [Re: LozMyKeys]
    #27702245 - 03/20/22 09:30 AM (1 year, 10 months ago)

If it's just getting worse with every transfer, it's not likely to randomly start getting better.  Start a new spore plate and make sure your initial transfers are from clean colonies.  Streak your spores to the plate so you can easily distinguish the different colonies.


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OfflineLozMyKeys
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Re: What mould is this? Enmeshed? [Re: PBJ710]
    #27702247 - 03/20/22 09:33 AM (1 year, 10 months ago)

Quote:

PBJ710 said:
If it's just getting worse with every transfer, it's not likely to randomly start getting better.  Start a new spore plate and make sure your initial transfers are from clean colonies.  Streak your spores to the plate so you can easily distinguish the different colonies.




They started out looking very clean. Then some began to have the halo around them and growth would slow. So how do I know what's clean and what's not at the beginning? Very hard to see by eye until it gets to this stage


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OfflineA.k.aM
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Re: What mould is this? Enmeshed? [Re: LozMyKeys]
    #27702281 - 03/20/22 10:31 AM (1 year, 10 months ago)

Is it just this culture??

I had a very similar issue that was caused by my plates. Something was off with a batch of agar and every culture grew slow and thin like that. As soon as I moved them to new plates with good agar they were fine.


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OfflinePBJ710
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Re: What mould is this? Enmeshed? [Re: LozMyKeys]
    #27702291 - 03/20/22 10:43 AM (1 year, 10 months ago)

Quote:

LozMyKeys said:
Quote:

PBJ710 said:
If it's just getting worse with every transfer, it's not likely to randomly start getting better.  Start a new spore plate and make sure your initial transfers are from clean colonies.  Streak your spores to the plate so you can easily distinguish the different colonies.




They started out looking very clean. Then some began to have the halo around them and growth would slow. So how do I know what's clean and what's not at the beginning? Very hard to see by eye until it gets to this stage




I just hold them up to a strong light source and look at them from both sides.  Look for fast growing mycelium that's along the streaks and take a transfer from the very leading edge of growth in a clean part of the plate.  If you blast MS on a plate and let growth completely cover the surface, it makes reliably selecting a clean initial transfer more difficult.

Agar recipe *can* alter the growth of mycelium, but it's generally consistent to all growth on the plate from my experiences.  What recipe and % was used?


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OfflineLozMyKeys
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Re: What mould is this? Enmeshed? [Re: A.k.a]
    #27702293 - 03/20/22 10:44 AM (1 year, 10 months ago)

No actually I'm running 4 at the moment and all have been this very weak whispy mycelium. Lots of slow growth they all seem to have the "halo" thing. All have been really thin mycelium up until T3 where I've seen rhizo growth towards the edge of the plate. Now on T4 and they look like this. A transfer from another culture on these new green plates however looked really strong and thick but got contaminated so did a quick transfer of that today so I'll see how they go.

I buy my plates premade as I don't really have the means to do it myself right now as I'm living with family. Would love to try and do it myself eventually because its getting expensive and before I know it I'm out of plates and have nowhere near the strong/clean growth I want so need to buy more


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OfflineLozMyKeys
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Re: What mould is this? Enmeshed? [Re: LozMyKeys]
    #27702299 - 03/20/22 10:52 AM (1 year, 10 months ago)

Here's how it started:

Swabs to plate looked strong to start and grew super quick and really thick.



Then after taking transfers and letting grow out they looked like this:




Then I started seeing some rhizo strands so i transfered those



then plates started looking like this:




now this new batch of plates I got I got the really weird looking mould slime stuff but one of my cultures was like this looked really thick and strong but got contaminated.

I took some transfers from this one today


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Invisiblesandman420
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Re: What mould is this? Enmeshed? [Re: LozMyKeys]
    #27702342 - 03/20/22 11:30 AM (1 year, 10 months ago)

your last picture makes it pretty clear that the mold makes the big fat clear mycelium on your green agar mix first.


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OfflineLozMyKeys
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Re: What mould is this? Enmeshed? [Re: sandman420]
    #27702344 - 03/20/22 11:34 AM (1 year, 10 months ago)

Quote:

sandman420 said:
your last picture makes it pretty clear that the mold makes the big fat clear mycelium on your green agar mix first.



in english please?


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OfflinePBJ710
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Re: What mould is this? Enmeshed? [Re: LozMyKeys] * 1
    #27702348 - 03/20/22 11:39 AM (1 year, 10 months ago)

Both of those starter plates are full of mold IMO - not worth trying to use.

I speak a little jive, but I couldn't quite figure out what he meant either :shrug:


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Offlineshuna
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Re: What mould is this? Enmeshed? [Re: PBJ710]
    #27702361 - 03/20/22 11:52 AM (1 year, 10 months ago)

Quote:

PBJ710 said:
Both of those starter plates are full of mold IMO - not worth trying to use.

I speak a little jive, but I couldn't quite figure out what he meant either :shrug:




:whathesaid:

how are you sterilising your scalpel?


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OfflineLozMyKeys
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Re: What mould is this? Enmeshed? [Re: shuna]
    #27702366 - 03/20/22 11:55 AM (1 year, 10 months ago)

Quote:

PBJ710 said:
Both of those starter plates are full of mold IMO - not worth trying to use.

I speak a little jive, but I couldn't quite figure out what he meant either :shrug:



Quote:

shuna said:
Quote:

PBJ710 said:
Both of those starter plates are full of mold IMO - not worth trying to use.

I speak a little jive, but I couldn't quite figure out what he meant either :shrug:




:whathesaid:

how are you sterilising your scalpel/loop?




Ye the one with the swab in looks like there might be some of that clear mould already but the top looked like spore germination to me. Hard to tell. I have a whole bunch of pre-sterilised tattoo needles that I use. I flame them before every transfer


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Offlineshuna
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Re: What mould is this? Enmeshed? [Re: LozMyKeys]
    #27702372 - 03/20/22 11:59 AM (1 year, 10 months ago)

like a disposable lighter?


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OfflineLozMyKeys
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Re: What mould is this? Enmeshed? [Re: shuna]
    #27702388 - 03/20/22 12:23 PM (1 year, 10 months ago)

Quote:

shuna said:
like a disposable lighter?




Ye it’s a disposable lighter I wipe it down with iso before use and sometimes in between transfers. But if there’s mould on the first plate from swab I was obviously doomed to start. How do I know if all my swabs don’t have this? And I know swabs and spores are inherently dirty so “cleaning” them up through transfers is the idea but that’s what I’ve been doing and I’m struggling to shake this stuff.

It’s strange tho you see the last picture of the green plate, that’s the first time since working with these cultures that I’ve seen some solid thick growth like that. And I don’t see any of the clear stuff on that one


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Invisiblesandman420
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Re: What mould is this? Enmeshed? [Re: LozMyKeys]
    #27702392 - 03/20/22 12:27 PM (1 year, 10 months ago)

If you look at the mold satellite on your last pic you can see the large "clear" outer circle that is also present on your transfers.


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OfflineLozMyKeys
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Re: What mould is this? Enmeshed? [Re: sandman420]
    #27702397 - 03/20/22 12:32 PM (1 year, 10 months ago)

Quote:

sandman420 said:
If you look at the mold satellite on your last pic you can see the large "clear" outer circle that is also present on your transfers.




If you’re referring to the trich like green mould that is not the same as the ‘clear’ stuff on the other plates. That’s a completely separate contamination


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Invisiblesandman420
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Re: What mould is this? Enmeshed? [Re: LozMyKeys]
    #27702426 - 03/20/22 01:10 PM (1 year, 10 months ago)

it is a separate contamination but you can see the big clear growth is what it does, and see it on your other plates.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


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OfflineLozMyKeys
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Re: What mould is this? Enmeshed? [Re: sandman420]
    #27702445 - 03/20/22 01:26 PM (1 year, 10 months ago)

Quote:

sandman420 said:
it is a separate contamination but you can see the big clear growth is what it does, and see it on your other plates.




There is clear growth around the green but I’m pretty certain it’s not the same thing. If it was I’m
sure I would have seen green on many more of my plates


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Invisiblesandman420
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Re: What mould is this? Enmeshed? [Re: LozMyKeys]
    #27702483 - 03/20/22 02:06 PM (1 year, 10 months ago)

Not really. Through enzymes in the metabolites or other unfavorable conditions the parasite won't always show sporulation on agar plates.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


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Offlineshuna
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Re: What mould is this? Enmeshed? [Re: sandman420]
    #27702497 - 03/20/22 02:26 PM (1 year, 10 months ago)

There's someone in the marketplace offering international random cube prints. I use a meth spirit burner made out of baby food jar and cotton ball wick, or mybe a butane gas torch. lighter doesn't get hot enough imo.

just curious what your gonna send the agar wedge to with no PC access, brf cake? spider man tek?


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OfflineLozMyKeys
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Re: What mould is this? Enmeshed? [Re: shuna] * 1
    #27702513 - 03/20/22 02:47 PM (1 year, 10 months ago)

Quote:

shuna said:
There's someone in the marketplace offering international random cube prints. I use a meth spirit burner made out of baby food jar and cotton ball wick, or mybe a butane gas torch. lighter doesn't get hot enough imo.

just curious what your gonna send the agar wedge to with no PC access, brf cake? spider man tek?




I buy pre-sterilised grain bags, and drop my wedges into there then send to bulk in a monotub.


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OfflineLozMyKeys
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Re: What mould is this? Enmeshed? [Re: LozMyKeys]
    #27704431 - 03/22/22 10:02 AM (1 year, 10 months ago)

Update:

The mycelium seems to be coming through thick on these plates now, its out running whatever this stuff is. Still undecided whether the clear stuff was just the beginnings of a weak mycelium. Mixed reviews from everyone. Anyway here are the pictures for those interested.







I was ready to give up on these plates too but I thought I'd let them ride it out and see how they cope. I'll let them grow out a bit more before I take another transfer.


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