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SharaVabdas



Registered: 03/23/22
Posts: 489
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Re: Plant cell culture *DELETED* [Re: clueless]
#27723093 - 04/06/22 10:38 AM (2 years, 1 month ago) |
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Post deleted by SharaVabdas
Reason for deletion: Shroomery is full of bigots and I'm leaving.
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7Suns
Researcher



Registered: 05/11/14
Posts: 765
Loc: not present
Last seen: 1 hour, 41 minutes
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I’m going to do a write up on my media recipe and post the link here, been busy but it’s in the works just need a little more time, maybe this weekend after the farmers market I’ll finish it if I sell out before 12 I should have enough time afterwards
-------------------- Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage 7suns grain sac hack 7suns straw log hack 7suns official OTC antibiotic agar recipe 7suns DIY filter bags 7suns cheap AF flowhood 7suns cold weather fruit chamber
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SharaVabdas



Registered: 03/23/22
Posts: 489
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Re: Plant cell culture *DELETED* [Re: 7Suns]
#27723125 - 04/06/22 11:06 AM (2 years, 1 month ago) |
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Post deleted by SharaVabdas
Reason for deletion: Shroomery is full of bigots and I'm leaving.
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bambus
Amphibian furniture maker



Registered: 05/03/11
Posts: 406
Last seen: 1 hour, 55 minutes
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I can't wait to hear more about the home made recipe for this. @7suns, What do you do with your chanterelle cultures? I've tried to clone them unsuccessfully, but wasn't sure what to do with it after I got it on a plate anyways.
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CreonAntigone
Stranger

Registered: 05/30/21
Posts: 2,970
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Re: Plant cell culture [Re: bambus]
#27730938 - 04/12/22 12:25 AM (2 years, 1 month ago) |
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Quote:
bambus said: wasn't sure what to do with it after I got it on a plate anyways.
I suggest 'in vitro mycorhizal snythesis' - illustrated here -

Isolate the fungal culture, propagate as LC to increase the volume. Then mix the pure culture with a mycorhizal partner invitro -
so for example, chanterelles are partners with birch and oak. So soak oak acorns in chanterelle liquid culture, and similarly you can put rooted oak cuttings in a jar of chanterelle culture. Wait for the roots to mycorhize indicated by the new white growth. Then plant in a greenhouse or just right outside.
It is the same principle behind growing pure culture, except it is two instead of one - dual culture, the trees and the chanterelles together.
It may take several attempts for the cuttings to survive. What you can do is start some saplings in a greenhouse that are chaterelle-partnered, then plant a set of new plants inoculated with a different liquid culture (ie genetics derived from a different fruit body). It seems in my experience this strain-strain competition increases the growth of the mycorhizal species.
And if the first set of cuttings doesn't work, you might that next season they do. I have been trying to mycorhize quaking aspen, the first attempt all cuttings died, this second attempt every single cutting rooted, lived and grew. It is a learning process.
Working with old trees helps - you can inoculate them with quite a few strains and they'll survive and be fine. Yes old trees can be inoculated, they just take a stronger innoculant owing to their maturity - one can just mass-produce a liquid culture of the mycorhizael sp. and pour it on a tree, which is a very simple method of inoculation but nonetheless, effective and sustainable.
This should establish oak trees that are partners with chanterelle. But it still would take a long time, potentially years, before there is any fruiting. It is still worth doing. There's that saying about 'the best time to plant a tree'.
Edited by CreonAntigone (04/12/22 12:26 AM)
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bambus
Amphibian furniture maker



Registered: 05/03/11
Posts: 406
Last seen: 1 hour, 55 minutes
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Wow! Thanks for the detailed response. I have a great chanterelle spot, and a bunch of oaks on my land, so I'll have to give this a try at some point.
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CreonAntigone
Stranger

Registered: 05/30/21
Posts: 2,970
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Re: Plant cell culture [Re: bambus]
#27732746 - 04/13/22 11:54 AM (2 years, 1 month ago) |
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Quote:
bambus said: Wow! Thanks for the detailed response. I have a great chanterelle spot, and a bunch of oaks on my land, so I'll have to give this a try at some point.
So the way it works is, if there is a chanterelle spot, those trees are chanterelle trees - the way the partnership works is that fruiting happens at the partnership's strongest points.
So, what trees are next to your chanterelle spot? Take cuttings or samples of those trees, as they already carry chanterelle culture in them and already are in partnership.
Then you can plant these natural chanterelle trees next to ones you synthesized invitro (as in my last post) - plant them every other tree. This would create strain-strain competition.
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bambus
Amphibian furniture maker



Registered: 05/03/11
Posts: 406
Last seen: 1 hour, 55 minutes
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They're Oak trees, not sure what kind though. I'm pondering the idea of collecting some acorns from that spot, sprouting them and trying to inoculate them immediately as they sprout. If they're then planted in a nutrient poor soil, maybe they'll be more inclined to bond through necessity? The native soil is heavy clay.
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CreonAntigone
Stranger

Registered: 05/30/21
Posts: 2,970
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Re: Plant cell culture [Re: bambus]
#27734912 - 04/14/22 07:37 PM (2 years, 1 month ago) |
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Quote:
bambus said: They're Oak trees, not sure what kind though. I'm pondering the idea of collecting some acorns from that spot, sprouting them and trying to inoculate them immediately as they sprout. If they're then planted in a nutrient poor soil, maybe they'll be more inclined to bond through necessity? The native soil is heavy clay.
I do think a mycorrhizal association can make up for deficits in the soil.
Mycorrhizal species seem to prefer loam which is about in the middle when it comes to soil types. But clearly there are plenty of natural chanterelles that come up in your spot, so I'm going to guess that your soil is fine for chanterelles.
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