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InvisibleCreonAntigone
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Plant cell culture
    #27698184 - 03/16/22 08:17 PM (2 years, 2 months ago)

Maybe 'advanced botany' as opposed to mycology, but -

I see that many labs grow plant cells in culture media. How do they go about this? What media could be used? What kind of nutrients might a plant need that could be added to culture media?

I have read about the growth of moss in culture. But moss is a special case as it has spores. How would I go about culturing, say, a tomato plant? Could I go from a tomato leaf to a culture plate of tomato cells?

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OfflineMrBlueshrooms
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Re: Plant cell culture [Re: CreonAntigone]
    #27698273 - 03/16/22 10:00 PM (2 years, 2 months ago)

There is a book I read many years ago called plants from test tubes that describes this. Generally you don't culture cells, but explants.  Cells can be cultured though by using physical methods to get the cells (like a blender) or chemicals or enzymes. Media is more complicated but sterile technique is the same.
To get tissue into agar several washes and rinses  are used. I think diluted bleach and water iso and dish soap did the trick.

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Offlinehazyhorse
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Re: Plant cell culture [Re: MrBlueshrooms]
    #27698418 - 03/17/22 03:06 AM (2 years, 2 months ago)

i saw an old thread about this recently. it sounds cool, but what is the point of doing this? i just didn't understand the practical purpose of it & no one really mentioned WHY they were talking about it. can you grow more plants from it or something?


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Invisiblepablokabute
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Re: Plant cell culture [Re: hazyhorse]
    #27698432 - 03/17/22 03:26 AM (2 years, 2 months ago)

Quote:

hazyhorse said:
i saw an old thread about this recently. it sounds cool, but what is the point of doing this? i just didn't understand the practical purpose of it & no one really mentioned WHY they were talking about it. can you grow more plants from it or something?





In my country, Most, if not all, banana plant cultures for farming bananas are from tissue culture and most of the time are affordable(but when you need, say, a thousand plants or more, then it quickly adds up) so my guess is,depending on what specific plant youre trying to grow, it might be practical.

Yes you could probably grow way more plants vs traditional propagation methods.


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: โ€œAll CONTAMINATED things and events are unsatisfactory.โ€'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

--Anonymous Guerilla, or is he..

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Offlinehazyhorse
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Re: Plant cell culture [Re: pablokabute]
    #27698870 - 03/17/22 01:03 PM (2 years, 2 months ago)

thatโ€™s super interesting!! i didnโ€™t realize it was such a viable propagation method. iโ€™ll have to look more into it, the concept is pretty cool


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

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Invisiblepablokabute
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Re: Plant cell culture [Re: hazyhorse]
    #27699288 - 03/17/22 06:47 PM (2 years, 2 months ago)

Quote:

hazyhorse said:
thatโ€™s super interesting!! i didnโ€™t realize it was such a viable propagation method. iโ€™ll have to look more into it, the concept is pretty cool





You can search for 'micropropagation'.

Id also like to learn to do it someday. :smile:


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: โ€œAll CONTAMINATED things and events are unsatisfactory.โ€'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

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Offlineclueless
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Re: Plant cell culture [Re: pablokabute]
    #27699331 - 03/17/22 07:27 PM (2 years, 2 months ago)

I've been doing tissue culture work for a few years now. Murashige and Skoog media is most commonly used, but orchids and walnuts have their own formulas. As for coming from a stem or leaf, that's absolutely possible.


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InvisibleCreonAntigone
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Re: Plant cell culture [Re: pablokabute]
    #27699439 - 03/17/22 08:47 PM (2 years, 2 months ago)

Quote:

hazyhorse said:
i saw an old thread about this recently. it sounds cool, but what is the point of doing this? i just didn't understand the practical purpose of it & no one really mentioned WHY they were talking about it. can you grow more plants from it or something?




One of my intentions is to explore it for mycorrhizal or parasitic fungal species that need plants to live. Some species only grow with plants and won't germinate on MEA. So the idea would be 'co-culturing', both fungi and plant cells.

Example: there is a fungus that lives on the leaves of morning glory plants and makes alkaloids. It was tested on sterile media and it didn't grow. One might be able to produce alkaloids though in a dish where you grow out morning glory leaf cells.

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OfflineSingularFusion
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Re: Plant cell culture [Re: CreonAntigone]
    #27699601 - 03/17/22 11:15 PM (2 years, 2 months ago)

I have this in my list of things to do sooner rather than later

The myriad applications for high value plants are pretty spectacular

Consider if you grow cannabis and have a couple dozen mother plants you keep as a genetic library, well that is using resources and grow space and obviously if you could keep most of that in cold storage and wake it as needed, that would be quite a saving of resources and of the daily effort to keep that all going in live plant form

Not to mention that now if you want backups kept to ensure the clone is never lost to law enforcement or sudden death, a friendly grower buddy is not needed anymore, anyone with a fridge can hold your cuts essentially. And with most folks not having the necessary skills to wake that up off a plate, the chance of theft is minimal, whereas taking a cutting from a live plant any idiot can do

Orchids are also interesting but that is a whole other discussion I don't know shit about... yet

Guess these techniques might be able to be applied to cacti as well, I dunno, hoping to learn more soon. I have seen there is added complexity to the plate media from the small amount of research I already did, I guess maybe some premixed media you buy is the way here, even though I would always make my own agar for mycology

good stuff fellas :rockon:


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Invisiblepablokabute
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Re: Plant cell culture [Re: SingularFusion]
    #27699617 - 03/17/22 11:43 PM (2 years, 2 months ago)

Quote:

Nef said:
I have this in my list of things to do sooner rather than later

The myriad applications for high value plants are pretty spectacular

Consider if you grow cannabis and have a couple dozen mother plants you keep as a genetic library, well that is using resources and grow space and obviously if you could keep most of that in cold storage and wake it as needed, that would be quite a saving of resources and of the daily effort to keep that all going in live plant form

Not to mention that now if you want backups kept to ensure the clone is never lost to law enforcement or sudden death, a friendly grower buddy is not needed anymore, anyone with a fridge can hold your cuts essentially. And with most folks not having the necessary skills to wake that up off a plate, the chance of theft is minimal, whereas taking a cutting from a live plant any idiot can do

Orchids are also interesting but that is a whole other discussion I don't know shit about... yet

Guess these techniques might be able to be applied to cacti as well, I dunno, hoping to learn more soon. I have seen there is added complexity to the plate media from the small amount of research I already did, I guess maybe some premixed media you buy is the way here, even though I would always make my own agar for mycology

good stuff fellas :rockon:



Thanks for all the info, this is the kind of info Im trying to absorb from this site! Cheers.
:mindblown:


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: โ€œAll CONTAMINATED things and events are unsatisfactory.โ€'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

--Anonymous Guerilla, or is he..

Edited by pablokabute (03/17/22 11:44 PM)

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InvisibleCreonAntigone
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Registered: 05/30/21
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Re: Plant cell culture [Re: SingularFusion]
    #27699659 - 03/18/22 12:45 AM (2 years, 2 months ago)

Quote:

clueless said:
I've been doing tissue culture work for a few years now. Murashige and Skoog media is most commonly used, but orchids and walnuts have their own formulas. As for coming from a stem or leaf, that's absolutely possible.




Quote:

Nef said:
I have seen there is added complexity to the plate media from the small amount of research I already did, I guess maybe some premixed media you buy is the way here, even though I would always make my own agar for mycology





Yeah I'm sure these special medias provide the best results... but I would highly doubt if there wasn't something that could be made in a home pretty easily. This site seems to have taught me that a lot of complex cultivation tools have a DIY equivalent. I guess it needs testing.

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Offline7SunsS
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Re: Plant cell culture [Re: CreonAntigone]
    #27700169 - 03/18/22 12:58 PM (2 years, 2 months ago)

Hey guys I was actually just about to do a write up on a home made version of the murashige and skoog or modified fries medium that I concocted from published science articles

Iโ€™m deep into the study of ecto/mycorrhizal species and this is something Iโ€™ve been trying to mimic in house so Iโ€™ll share the link as soon as I finish the write up.... everything can be bought locally except ph paper

Also if youโ€™re going this route I also recommend a quick look at my post about ph effects on agar solidification

https://www.shroomery.org/forums/showflat.php/Number/27696637


Also hereโ€™s my home made antibiotic agar recipe if you are starting from a wild specimen itโ€™s very helpful since itโ€™s more then likely heavily contaminated with bacteria especially chanterelles that can have bacteria incorporated internally

https://www.shroomery.org/forums/showflat.php/Number/27576801


--------------------
Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage
7suns grain sac hack
7suns straw log hack
7suns official OTC antibiotic agar recipe
7suns DIY filter bags
7suns cheap AF flowhood
7suns cold weather fruit chamber

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Offline7SunsS
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Re: Plant cell culture [Re: 7Suns]
    #27700171 - 03/18/22 12:59 PM (2 years, 2 months ago)

Also there a Facebook page I believe itโ€™s called the โ€œcactus laboratoryโ€
Where the guy uses this medium for rooting cactus cuttings, really fascinating work


--------------------
Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage
7suns grain sac hack
7suns straw log hack
7suns official OTC antibiotic agar recipe
7suns DIY filter bags
7suns cheap AF flowhood
7suns cold weather fruit chamber

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Offlinehazyhorse
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Re: Plant cell culture [Re: 7Suns]
    #27700290 - 03/18/22 02:45 PM (2 years, 2 months ago)

fuck yeah, this is really cool. glad i found this thread! some home DIY methods for getting into this kind of work would be really awesome. the example of keeping a library of mother plants alone would be so practical & worth it. iโ€™m keeping one as a โ€œbonsaiโ€ mother right now but fuck, she grows like 6โ€ in a week lol. plus itโ€™s so easy to under water once they get root bound, not to mention nutrients etc. the cacti applications are also quite fascinating. would love to go down this rabbit hole more


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ

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Offlinenerdintheshell
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Re: Plant cell culture [Re: CreonAntigone]
    #27703018 - 03/21/22 01:13 AM (2 years, 1 month ago)

For media murashige and skoog agar seems to be a common one. The ingredient list is pretty varied and may not be as easy to make at home as other mediums like pda or mea.

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Invisiblepablokabute
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Re: Plant cell culture [Re: nerdintheshell]
    #27703027 - 03/21/22 01:53 AM (2 years, 1 month ago)

Quote:

nerdintheshell said:
For media murashige and skoog agar seems to be a common one. The ingredient list is pretty varied and may not be as easy to make at home as other mediums like pda or mea.





Which reminds me of overcomplicated Cordyceps liquid culture recipes and yet its progressing to somewhat more accessible ingredients now.


Maybe if more people catches on to doing plant cell cultures, easier, more accesible recipes will be opened up?


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: โ€œAll CONTAMINATED things and events are unsatisfactory.โ€'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

--Anonymous Guerilla, or is he..

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Offline7SunsS
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Re: Plant cell culture [Re: pablokabute]
    #27703135 - 03/21/22 06:57 AM (2 years, 1 month ago)

Did you guys not read my comment?

I have a simple home recipe I created as a substitute for this recipe


--------------------
Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage
7suns grain sac hack
7suns straw log hack
7suns official OTC antibiotic agar recipe
7suns DIY filter bags
7suns cheap AF flowhood
7suns cold weather fruit chamber

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Invisiblepablokabute
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Registered: 11/22/11
Posts: 5,187
Loc: rural ghetto
Re: Plant cell culture [Re: 7Suns]
    #27703188 - 03/21/22 07:54 AM (2 years, 1 month ago)

Quote:

7Suns said:
Did you guys not read my comment?

I have a simple home recipe I created as a substitute for this recipe




I only saw low pH agar and antibiotic agar recipes, is that what youre referring to?


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: โ€œAll CONTAMINATED things and events are unsatisfactory.โ€'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

--Anonymous Guerilla, or is he..

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Offline7SunsS
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Re: Plant cell culture [Re: 7Suns]
    #27703203 - 03/21/22 08:07 AM (2 years, 1 month ago)

Quote:

7Suns said:
Hey guys I was actually just about to do a write up on a home made version of the murashige and skoog or modified fries medium that I concocted from published science articles

Iโ€™m deep into the study of ecto/mycorrhizal species and this is something Iโ€™ve been trying to mimic in house so Iโ€™ll share the link as soon as I finish the write up.... everything can be bought locally except ph paper


This โ˜๏ธ


--------------------
Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage
7suns grain sac hack
7suns straw log hack
7suns official OTC antibiotic agar recipe
7suns DIY filter bags
7suns cheap AF flowhood
7suns cold weather fruit chamber

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OfflineSingularFusion
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Re: Plant cell culture [Re: 7Suns]
    #27703242 - 03/21/22 08:49 AM (2 years, 1 month ago)

look forward to hearing your take on it 7suns :cheers:


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