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nektar61
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Re: DIY Psilocybin Content Percentage Detection [Re: Icyurmt] 1
#27758239 - 04/30/22 11:48 PM (1 year, 8 months ago) |
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Quote:
Icyurmt said: If not marquis perhaps it's mecke?
I think it's Marquis. Might even be that and nothing else.
I ordered some marquis along with some Ehrlich.
I don't know if you saw the chart I posted on the previous page, but the two actives in shrooms, one turns Marquis brown and one turns yellow. Dark yellow liquids look brown.
--
Quote:
Icyurmt said: I know mecke also reacts with sugars so you would likely want to use an extract solvent that accounted for that if possible.?
One of the cop drug test links I posted earlier on this thread mentioned extracting with methanol then washing with acetone to remove sugars, but that was prepping for GC. https://www.swgdrug.org/Monographs/PSILOCIN.pdf
That page also mentions using Froehde’s Reagent. Turns yellow (eventually) for both alkeloids. Again, dark yellow liquids look brown.
The PsiloQ test produces brown.
"The Froehde reagent is used as a simple spot-test to presumptively identify alkaloids, especially opioids, as well as other compounds. It is composed of a mixture of molybdic acid or a molybdate salt dissolved in hot, concentrated sulfuric acid, which is then dripped onto the substance being tested.
"The United States Department of Justice method for producing the reagent is the addition of 100 ml of hot, concentrated (95–98%) sulfuric acid to 0.5 g of sodium molybdate or molybdic acid."
"The Virginia Department of Forensic Science method uses 0.5 g ammonium molybdate per 100 ml H2SO4 (conc.)[2]
"Unheated sulfuric acid can be used to prepare the reagent in a less dangerous manner, but 2–4 hours must be allowed for the molybdate to dissolve." https://en.wikipedia.org/wiki/Froehde_reagent
That's an interesting one. Turns a lot of different colors, can test presence of a lot of types of drugs. Look at that link on the wikipedia link.
I'm going by the guess that anything that can change color to detect presence of something might change color more in the presence of more of that thing. Some reagents maybe more than others.
-------------------- -NEW? Start here.
Edited by nektar61 (05/01/22 12:01 AM)
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Forrester
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Re: DIY Psilocybin Content Percentage Detection [Re: nektar61] 2
#27758372 - 05/01/22 04:54 AM (1 year, 8 months ago) |
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Quote:
nektar61 said: There is some first-hand (non-scientific) info on Shroomery of someone storing non-ground shrooms in a hot garage for 10 years and still having them be very strong.
Forrester's quote here, in the vacuum pack and long-term storage thread: https://www.shroomery.org/forums/showflat.php/Number/27660424#27660424
Seems to imply that a warm garage is less damaging to potency than air is. I'll ask him to clarify. (He did not vacuum pack, he stored in a jar, but did not grind the shrooms.)
I've probably seen 30 people on here recommend against grinding shrooms for storage....all say it degrades potency quicker.
Yeah I think it's pretty well accepted that it's oxidation that degrades the actives over time, not temperature. My experiment was very anecdotal of course, but during that ten years they were stored, the jars were moved several times between hot garages and basements (I lived in 3 different places over the period) so the temperature was far from stable. I think the chitin cell walls of un-ground fruit bodies keep the actives pretty well protected from oxidation, and wide swings in temperature didn't seem to cause any issues. Vacuum packing would obviously be an even better way to do it, to get the oxygen out of there, but I'm not sure how much difference it makes over the long term as long as the fruit bodies are intact.
If you're interested in the "purging with an inert gas" idea, you can always just buy one of these:
wine preserver
But I wouldn't waste the money for storing mushrooms. This stuff was recommended by a few on here years ago for storing other powders (RC's mostly) to keep them protected from oxidation. It would be interesting to see if it worked to help extend the shelf life of DMT freebase which is pretty short.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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nektar61
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Re: DIY Psilocybin Content Percentage Detection [Re: Forrester] 1
#27760386 - 05/02/22 06:30 PM (1 year, 8 months ago) |
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I confirmed with Forrester via email, the shrooms that lasted 10 years in varying amounts of heat with no noticeable degradation of potency were NOT ground (as I suspected), they were whole.
They were not PE, probably B+. Someone else said PE on the long-term storage thread.
-------------------- -NEW? Start here.
Edited by nektar61 (05/02/22 08:18 PM)
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nektar61
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Re: DIY Psilocybin Content Percentage Detection [Re: nektar61] 1
#27760569 - 05/02/22 08:19 PM (1 year, 8 months ago) |
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Does anyone (A-Rock?) know if psilocin is soluble in Isopropyl alcohol?
I'm thinking 70% ISO would be better extraction solvent for testing shrooms, especially since growers have it on hand.
Methanol is VERY toxic, not something people who don't know chemistry should play with....at least not the wide group of people we'd be aiming for with an open-source grower's test.
The 30% water in 70% ISO would dissolve the Psilocybin, and I'm hoping the ISO would dissolve the psilocin.
Most chemistry sites that have profiles of chemicals list solubility in everything else: water, methanol, ethanol, acetone, ether. Not ISO.
Web search mostly points to Shroomery extractions. haha. This extraction said it made a strong goo, but could have just been https://www.shroomery.org/forums/showflat.php/Number/15982455
Most tests use methanol. According to this: https://www.swgdrug.org/Monographs/PSILOCIN.pdf
psilocin dissolves in methanol, but psilocybin is only slightly soluble in methanol. If that's the case, common tests are mostly only testing the psilocybin, not much of the psilocin.
I'm sure we want to test both.
Of course Everclear (ethanol and water) would work, and work well. But Iso alcohol 70% is what most of us already have on hand.
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SpectreOfCommunism
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Re: DIY Psilocybin Content Percentage Detection [Re: nektar61] 2
#27761942 - 05/03/22 08:11 PM (1 year, 8 months ago) |
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Quote:
nektar61 said: Does anyone (A-Rock?) know if psilocin is soluble in Isopropyl alcohol?
I'm thinking 70% ISO would be better extraction solvent for testing shrooms, especially since growers have it on hand.
Methanol is VERY toxic, not something people who don't know chemistry should play with....at least not the wide group of people we'd be aiming for with an open-source grower's test.
The 30% water in 70% ISO would dissolve the Psilocybin, and I'm hoping the ISO would dissolve the psilocin.
Most chemistry sites that have profiles of chemicals list solubility in everything else: water, methanol, ethanol, acetone, ether. Not ISO.
Web search mostly points to Shroomery extractions. haha. This extraction said it made a strong goo, but could have just been https://www.shroomery.org/forums/showflat.php/Number/15982455
Most tests use methanol. According to this: https://www.swgdrug.org/Monographs/PSILOCIN.pdf
psilocin dissolves in methanol, but psilocybin is only slightly soluble in methanol. If that's the case, common tests are mostly only testing the psilocybin, not much of the psilocin.
I'm sure we want to test both.
Of course Everclear (ethanol and water) would work, and work well. But Iso alcohol 70% is what most of us already have on hand.
Psilocybin and psilocin are BOTH soluble in water and methanol. Both are strongly polar molecules that will dissolve in most polar solvents and will not dissolve in nonpolar solvents like petroleum ether, naphtha, etc. Think about it — if psilocin wasn’t soluble in water, then mushroom tea made from fresh mushrooms would be no more potent than mushroom tea made with 1/10 of that weight of dried mushrooms because fresh mushrooms are more potent primarily because of their psilocin content which is largely destroyed by the drying process. Also you can literally watch psilocin oxidize in an aqueous solution because that aqueous solution will turn blue. Psilocin is slightly MORE soluble than psilocybin in methanol and psilocybin is slightly MORE soluble than psilocin in water but they are both soluble in polar solvents in general.
Also, isopropyl alcohol is toxic too btw and ordinary people handle methanol all the time whenever they put gas-line antifreeze and water-removers like HEET into their gas tank. Or whenever people put winter-season wiper fluid in their cars. Or whenever people use denatured alcohol as a household solvent or paint cleaner. Just use gloves and don’t drink the stuff. Simple as that.
-------------------- Found Species: Ps. Azurescens, Ps. Cyanescens, Ps. Baeocystis, Ps. Semilanceata, Pan. Cinctulus
 
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nektar61
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Re: DIY Psilocybin Content Percentage Detection [Re: shr00med] 3
#27764174 - 05/05/22 10:03 AM (1 year, 8 months ago) |
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Here's results of my first test. I'm saving these on my Journal. ----
First test, May 5, 2022, Ehrlich's Reagent
Crushed up 1.28 grams of penis envy. Was harvested 3 months ago, was dried in dehydrator for 24 hours. Is strong, per my eating a gram a while back. Is not clone, is multispore, will be using clone for further tests.
Crushed with spoon on a plate (I've ordered an electric grinder). Put in test tube, added 20 ml Isopropyl alcohol 70%. (measured with syringe.)
Let sit at room temp for 6 hours. Shook 10 shakes each every 2 hours. Used a coffee glass as a test tube holder:
Filtered with sieve and funnel. Result is mostly clear straw-colored liquid.
Poured some into 4 small vials. Shook test tube 5 shakes between each pour.
Vial 1 got .5 ml solution. vial 2 got 1 ml solution. vial 3 got 2 ml solution. vial 4 got 3 ml solution. (all measured with syringe.)
Topped each off with 70% ISO so they were all 3 ml.
Unless I'm missing something, I think this step is important, otherwise you'll get different dilutions of the purple color reaction. Shook each one 10 shakes.
Added 10 drops Ehrlich's Reagent to each vial.
Shook vials all together 10 shakes at start, and every 15 min, including at end.
After one hour, result is inconclusive / paradoxical:
Doesn't seem to be much difference in purple tint, if anything, seems stronger 1, 2, and 3, which all had less material than 4.
Somehow maybe that IS the result? I don't know.
Will retry this test again at some point with 10 ml ISO instead of 20, to get a stronger solution to see color better.
Next, I will try Marquis reagent. I have higher hopes for that one, and did even before this test, based on reading.
(The remaining shroom / ISO extract in the test tube got thrown out. I'm going to mix fresh for each test.)
-------------------- -NEW? Start here.
Edited by nektar61 (05/05/22 10:28 AM)
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QM33
(NOT A PUPPET!) ❤❤❤❤❤



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Re: DIY Psilocybin Content Percentage Detection [Re: nektar61] 1
#27764314 - 05/05/22 12:20 PM (1 year, 8 months ago) |
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4 almost looks foggy no?
Maybe you maxed out the reagent.?
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joze



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Re: DIY Psilocybin Content Percentage Detection [Re: nektar61] 1
#27764456 - 05/05/22 01:51 PM (1 year, 8 months ago) |
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Quote:
nektar61 said: After one hour, result is inconclusive / paradoxical:
Doesn't seem to be much difference in purple tint, if anything, seems stronger 1, 2, and 3, which all had less material than 4.
Somehow maybe that IS the result? I don't know.
Will retry this test again at some point with 10 ml ISO instead of 20, to get a stronger solution to see color better.
Next, I will try Marquis reagent. I have higher hopes for that one, and did even before this test, based on reading.
(The remaining shroom / ISO extract in the test tube got thrown out. I'm going to mix fresh for each test.)
You're probably experiencing difficulties due to the alkalinity of the mushrooms. See SpectreOfCommunism's post I quoted below.
Quote:
SpectreOfCommunism said:
Quote:
joze said: Cool to see your progress. You should try this reaction at many different pH values.
I would recommend getting some pH indicator strips and testing the pH of your mushroom tea aftering boiling, before you add the reagent. Different amounts of mushroom may change the pH slightly. Buffer your mushroom tea to a set of different pH (perhaps pH 3, 5, 7, 9) and then carry out the reaction and see if different pH values lead to significantly different color changes. You may find that this reaction is much more sensitive if you buffer the reaction precisely.
Funny that you mention that because this was exactly my line of thinking this morning after observing two more trials with different ratios of extract to reagent. I’d expected a stronger reaction after increasing the amount of extract to 2ml but, when the opposite happened, I got to thinking and I’m pretty sure the result was due to me unwittingly raising the pH too much with the increased extract volume. I got out my pH test paper and sure enough the mushroom extract was more basic than I’d assumed. Once I get back home tonight, my plan is to use drops of phosphoric acid to adjust the pH of the extract so that it is slightly acidic before adding it to the reagent bottle. Does that seem sensible or would you suggest a different buffer?
I may also experiment with using methanol instead of water for the extract but one thing at a time…
--------------------
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nektar61
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Re: DIY Psilocybin Content Percentage Detection [Re: joze] 1
#27765058 - 05/05/22 08:11 PM (1 year, 8 months ago) |
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Quote:
joze said:
You're probably experiencing difficulties due to the alkalinity of the mushrooms. See SpectreOfCommunism's post I quoted below.
Thank you both.
So my shrooms need to take some acid?
All I have handy is vinegar. That good? Maybe 1 ml per 5 ml of solvent?
Is this going to make toxic gas? I think HCL and isopropanol make chlorine. I guess I already did that, since there is HCL in Erlich's. I did all this in a bathroom with the window open and a strong ceiling fan to vent.
Do you think I'll need the acid with other reagents? I have some Marquis now. That's formaldehyde and concentrated sulfuric acid.
-------------------- -NEW? Start here.
Edited by nektar61 (05/05/22 08:17 PM)
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joze



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Re: DIY Psilocybin Content Percentage Detection [Re: nektar61] 1
#27765145 - 05/05/22 09:08 PM (1 year, 8 months ago) |
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If you're experiencing the same effect, you'll likely need to buffer the pH regardless of the reagent you're using. I don't really know though, different reagents may be less sensitive to pH.
White vinegar (acetic acid) will work well, you could also make a dilute solution of HCl.
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nektar61
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Re: DIY Psilocybin Content Percentage Detection [Re: joze] 1
#27765193 - 05/05/22 09:58 PM (1 year, 8 months ago) |
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Quote:
joze said: If you're experiencing the same effect, you'll likely need to buffer the pH regardless of the reagent you're using. I don't really know though, different reagents may be less sensitive to pH.
White vinegar (acetic acid) will work well, you could also make a dilute solution of HCl.
Thank you.
I'll try white vinegar. I like to use what's on hand. Also, if I do anything worth sharing with other growers, I'd like to have as much of it as possible safer things from the supermarket.
Some people are reluctant to order chemicals, can get you on a list. I also still maintain that ISO is less toxic than methanol, despite SpectreOfCommunism saying it didn't matter.
Methanol is fine for them, for me, since I have a heart condition, the less toxic the better.
ISO is considered "relatively non-toxic."
On the other hand, "Ingesting as little as 10 mL of pure methanol can cause permanent blindness by destruction of the optic nerve."
I know he said "don't drink it", but someone will accidentally ingest (or even just inhale it), so I think for the common worker bee average shroom grower, less toxic is better, and less likely to get you on a list is better.
White vinegar is a better chemical for the average bear to be using than concentrated HCL (where diluting it could cause a spill or breathing fumes).
Most people have no idea how to safely work with common lab chemicals.
I know there are acids in the reagents, but we're not using much of the reagent, and we're diluting it, dropwise, into a larger amount of alcohol.
-------------------- -NEW? Start here.
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joze



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Loc: PNW
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Re: DIY Psilocybin Content Percentage Detection [Re: nektar61] 2
#27765873 - 05/06/22 11:53 AM (1 year, 8 months ago) |
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Quote:
nektar61 said:
Quote:
joze said:
You're probably experiencing difficulties due to the alkalinity of the mushrooms. See SpectreOfCommunism's post I quoted below.
Thank you both.
So my shrooms need to take some acid?
All I have handy is vinegar. That good? Maybe 1 ml per 5 ml of solvent?
Is this going to make toxic gas? I think HCL and isopropanol make chlorine. I guess I already did that, since there is HCL in Erlich's. I did all this in a bathroom with the window open and a strong ceiling fan to vent.
Do you think I'll need the acid with other reagents? I have some Marquis now. That's formaldehyde and concentrated sulfuric acid.
I just realized I didn't directly answer all of these questions.
In terms of toxicity, you shouldn't be generating anything seriously harmful. Having a window open and being mindful should keep you plenty safe. However, please avoid dumping your waste down a sink. DMAB is a pollutant. Keep a bucket of waste or something and find a waste disposal center.
For how much acid you should be using: I think it will take some trial and error to find the right amount to use, but you should be able to find out a constant pH that gives the strongest reaction with DMAB. You should get some pH test strips and see how much the mushroom tissue affects the pH. I have outlined a general procedure that I think would work well for you, based on your above procedure.
- Add sample and isopropyl (or whichever solvent you're using) to test tube. Let sample sit for 6 hours.
- Filter sample. Test sample pH and record.
- Use syringe to measure sample into test tube(s).
- Adjust sample pH dropwise with white vinegar. Re-test pH and buffer to ideal range (perhaps start with pH 6 or 7).
- Use syringe to adjust sample volume with isopropyl (if necessary).
- Add 10 drops of Ehrlich's reagent, shake, wait one hour.
- Record color change.
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nektar61
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Re: DIY Psilocybin Content Percentage Detection [Re: joze] 1
#27766368 - 05/06/22 06:15 PM (1 year, 8 months ago) |
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Quote:
joze said:
I just realized I didn't directly answer all of these questions....... [/LIST]
Thank you.
My next tests will be with Marquis agent and no extra acid, then I'll try both reagents again with extra acid, buffered to neutral. I have some pH strips.
-------------------- -NEW? Start here.
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nektar61
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Re: DIY Psilocybin Content Percentage Detection [Re: shr00med] 2
#27766934 - 05/07/22 06:44 AM (1 year, 8 months ago) |
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Test 2, Marquis Reagent
Ground dry strong cube clone (x7x) to fine with new grinder. Zeroed scale with paper before adding powder.

Added 20 mil ISO alcohol 70%
in 4 oz jar. Shook every hour for 6 hours:
Strained result, then filtered with coffee filter.
Lost some of it during filtering, so didn't have enough to do all planned tests. Just did 1 test, plus a part of another.
Vial 1: .5 ml shroom solution Vial 1: 1 ml shroom solution Vial 1: 2 ml shroom solution Vial 1: 3 ml shroom solution
On first 3, used more ISO to make up volume to 3 ml like vial 4.
Vial 5; 1.5 ml shroom solution + 1.5 ml white vinegar.
added 10 drops Marquis reagent to each vial:
Was an exothermic reactions. Vials got very warm to the touch soon after adding the reagent.
Shook every 15 min for 90 min. Result doesn't look useful. 1-4 are about the same color as before reagent. Maybe 4 is a little more yellow (which is one known reaction to psilocybin). But not enough to call it useful. Vial 5 looks milky and inconclusive.
I'm posting these because even non-results should be included, to avoid cherry picking results.

Next time I'll make it a point to photograph the before and after on the same background to make any differences more clear.
-------------------- -NEW? Start here.
Edited by nektar61 (05/07/22 07:16 AM)
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nektar61
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Re: DIY Psilocybin Content Percentage Detection [Re: shr00med] 1
#27768195 - 05/08/22 06:32 AM (1 year, 8 months ago) |
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Test 3, different things, and some promising results:
- I decided the thing to do was to try a few things, and look for ANY bold result...either brighter colors or very dark reaction.
Then do further tests next time to see if that can be tweaked to be quantitative.
First thing different from my previous tests: Instead of an alcohol, I tried a solution of an acid in water as the extractor. Something somewhere was telling me to do that (without the alcohol).
I had white vinegar on hand, I use it to clean my water distiller. It says on the label that it's adjusted to 5% acetic acid. The rest is mostly water.
Second new thing: I did a shorter extraction of my powdered shrooms, I did 2 hours instead of 6 hours. A test like this shouldn't take all day.
Third, fourth, and fifth new thing: Added heat. Also used more shrooms, and more extractor solution, so I'd have more to work with. Once I soaked the shrooms (3.24 grams of BBM clone) in 40 ml of white vinegar for 2 hours, I put the test tube in a glass of warm (90 f) water. Put it back there between pouring vials.
The extraction was very slightly blue, likely just shroom bluing reaction:
I used vials numbered from the last tests that I ended up not having enough extract to use last time. Vial number 7 is missing and was not used. This is the vials after adding reagent to only the first one (#6), but not the rest:
This is after adding different things. Got some dark results and some colorful results in 10 minutes.
They all have 1 ml of extraction, except the first, one, Vial 6, has 3 ml. (Once I started pouring, I realized I didn't have enough to put 3 ml in each one): LEGEND: Vial 6: 15 drops Ehrlich's Vial 8: 15 drops Marquis Vial 9: 5 drops Marquis Vial 10: 15 drops Ehrlich's Vial 11: 7 drops Ehrlich's, 7 drops Marquis, 7 drops Isopropyl alcohol 70% (water 30%) Vial 12: CONTROL. Nothing added.
This is at 15 minutes. Some color starting to fade.

This is at 1 hour. Purple on #10 and #11 mostly faded. 
I think Vial 10 (15 drops Ehrlich's) looks most promising with its purple result.
Though Vial 6 (15 drops Ehrlich's in 3x as much extract) and Vial 11 (7 drops Ehrlich's, 7 drops Marquis, 7 drops Isopropyl alcohol 70%) could be promising.
Interesting that the same amount of Ehrlich's in 3x as much solution turned brown, where in the 1 ml it looks purple.
Next test's goal would be to have stronger reaction of that purple or brown, then try with different amounts to see if can be tweaked to be quantitative.
Anyone who wants to jump in and try some, I'd love to see it, especially with stronger acids I don't feel comfortable using.
Might also add: -Heat. Warm water bath for the reaction.
--Check and photograph each minute for the first 15 minutes after adding reagent(s).
--Stronger extract? Counting loss from absorption into coffee filter used for filtering, and loss of absorption into shroom powder, I'd guess I ended up with something like 100 mg of shroom material per ml. Could double that.
--Stronger acid? I'm not going to mess with that, but some other people on here might.
--Try the extraction for 1 hour, not 2. I think most of the material might be extracted in 1 hour. Maybe not, but worth a try. The less this test costs, in time and money, the more useful it might be.
Note the Ehrlich's gives off heat when added to the extract. Also did when added to just ISO alcohol in previous tests. Might be reaction with the shroom components, not sure.
I'm saving used vials to neutralize outside with baking soda before sealing. Good idea? Will wear eye protection and good mask. Used both plus fan and open window for all my tests.
-------------------- -NEW? Start here.
Edited by nektar61 (05/08/22 12:44 PM)
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joze



Registered: 11/10/20
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Re: DIY Psilocybin Content Percentage Detection [Re: nektar61] 1
#27768544 - 05/08/22 11:24 AM (1 year, 8 months ago) |
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Awesome! Great progress nektar. I appreciate how well you're documenting your process.
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nektar61
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Re: DIY Psilocybin Content Percentage Detection [Re: joze]
#27768615 - 05/08/22 12:11 PM (1 year, 8 months ago) |
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Quote:
joze said: Awesome! Great progress nektar. I appreciate how well you're documenting your process.
Thank you. I try. What I lack in schooling I make up for in that my day job includes writing weekly reports on the whole warehouse, so organization is second nature. Though I did actually pay attention in high school chemistry, and I'm old enough that they used to teach chemistry in high school when I was there.
I'm wearing gloves, eye protection, a good mask and have a fan blowing everything out the window (or work outside when it's warm enough). Also keeping some baking soda solution handy in case any acid (like both of these reagents, or even the vinegar) gets on skin or in eyes. I got some of this vinegar on a paper cut a while back, hurt like the devil, and it's much weaker than the acids in the reagents.
Don't smoke or have flame around this. A little bit of hydrogen is being given off in that exothermic reaction.
For anyone wanting to jump in on these experiments and throw chemicals against the wall and see what sticks (hopefully based on some reading more than just a hunch), here's a possibly useful post I found on the Hive, chemicals you should not mix for one reason or another (usually because they result in either immediate fire, or immediate toxic gas): -----
Incompatible Chemicals By Boris
A wide variety of chemicals react dangerously when mixed with certain other materials. Some of the more widely-used incompatible chemicals are given below, but the absence of a chemical from this list should not be taken to indicate that it is safe to mix it with any other chemical!
acetic acid: chromic acid, ethylene glycol, nitric acid, hydroxyl compounds, perchloric acid, peroxides, permanganates acetone: concentrated sulphuric and nitric acid mixtures acetylene: chlorine, bromine, copper, fluorine, silver, mercury alkali and alkaline earth metals: water, chlorinated hydrocarbons, carbon dioxide, halogens, alcohols, aldehydes, ketones, acids aluminium (powdered): chlorinated hydrocarbons, halogens, carbon dioxide, organic acids. anhydrous ammonia: mercury, chlorine, calcium hypochlorite, iodine, bromine, hydrofluoric acid ammonium nitrate: acids, metal powders, flammable liquids, chlorates, nitrites, sulphur, finely divided organic combustible materials aniline: nitric acid, hydrogen peroxide arsenic compounds: reducing agents azides: acids bromine: ammonia, acetylene, butadiene, hydrocarbons, hydrogen, sodium, finely-divided metals, turpentine, other hydrocarbons calcium carbide: water, alcohol calcium oxide: water carbon, activated: calcium hypochlorite, oxidizing agents chlorates: ammonium salts, acids, metal powders, sulphur, finely divided organic or combustible materials chromic acid: acetic acid, naphthalene, camphor, glycerin, turpentine, alcohols, flammable liquids in general chlorine: see bromine chlorine dioxide: ammonia, methane, phosphine, hydrogen sulphide copper: acetylene, hydrogen peroxide cumene hydroperoxide: acids, organic or inorganic cyanides: acids flammable liquids: ammonium nitrate, chromic acid, hydrogen peroxide, nitric acid, sodium peroxide, halogens hydrocarbons: fluorine, chlorine, bromine, chromic acid, sodium peroxide hydrocyanic acid: nitric acid, alkali hydrofluoric acid: aqueous or anhydrous ammonia hydrogen peroxide: copper, chromium, iron, most metals or their salts, alcohols, acetone, organic materials, aniline, nitromethane, flammable liquids, oxidizing gases hydrogen sulphide: fuming nitric acid, oxidizing gases hypochlorites: acids, activated carbon iodine: acetylene, ammonia (aqueous or anhydrous), hydrogen mercury: acetylene, fulminic acid, ammonia mercuric oxide: sulphur nitrates: sulphuric acid nitric acid (conc.): acetic acid, aniline, chromic acid, hydrocyanic acid, hydrogen sulphide, flammable liquids, flammable gases oxalic acid: silver, mercury perchloric acid: acetic anhydride, bismuth and its alloys, ethanol, paper, wood peroxides (organic): acids, avoid friction or shock phosphorus (white): air, alkalies, reducing agents, oxygen potassium: carbon tetrachloride, carbon dioxide, water potassium chlorate: acids potassium perchlorate: acids potassium permanganate: glycerin, ethylene glycol, benzaldehyde, sulphuric acid selenides: reducing agents silver: acetylene, oxalic acid, tartaric acid, ammonium compounds, fulminic acid sodium: carbon tetrachloride, carbon dioxide, water sodium nitrate: ammonium salts sodium peroxide: ethanol, methanol, glacial acetic acid, acetic anhydride, benzaldehyde, carbon disulphide, glycerin, ethylene glycol, ethyl acetate, methyl acetate, furfural sulphides: acids sulphuric acid: potassium chlorate, potassium perchlorate, potassium permanganate (or compounds with similar light metals, such as sodium, lithium, etc.) tellurides: reducing agents zinc powder: sulphur Swio would have just posted the link, but it contains info on bomb making etc. and swio thinks that there is enough of these things going off around the world!
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Edited by nektar61 (05/08/22 12:34 PM)
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nektar61
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Re: DIY Psilocybin Content Percentage Detection [Re: shr00med]
#27769476 - 05/09/22 06:53 AM (1 year, 8 months ago) |
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I was wondering why I had two Erlich's tubes (different amounts of liquid / shroom alkaloids) where one with more was brownish and one with less was lavender.
I found something similar on an article called Quantitative Analysis of Psilocybin and Psilocin in Psilocybe Baeocytis byHigh-Performance Liquid Chromatography and by Thin-Layer Chromatography. Is about TLC but would these correspond to the simpler type we're doing here? If not, may help with those wanting to do home TLC:
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joze



Registered: 11/10/20
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Re: DIY Psilocybin Content Percentage Detection [Re: nektar61] 1
#27769908 - 05/09/22 02:53 PM (1 year, 8 months ago) |
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Hey nektar.
I've been doing some more reading on this topic, and I think you may actually have gone too acidic now, if you're using undiluted white vinegar to extract the mushroom tissue. In the paper "A Simplified Method for the Analysis of Hydroxyproline in Biological Tissues," (1996), the authors write that "A pH of the acetate-citrate buffer between 6.0 to 6.5 yielded maximal absorbance values for all samples. At pH values below 5, the formation of chromophore was found to be negligible; however, alkaline pH of the buffer did not influence the absorbance of standard Hyp except for those at pH 8.0."
This basically means that the DMAB reagent did not produce much of a color change when the sample was buffered below pH 5, and was optimal between pH 6 and 6.5. White vinegar is around pH 2.5, according to a quick Google search. I would mess around with using a couple of dilutions of white vinegar, maybe 1:1, 1:3, and 1:5 or something like that, and see how that effects the color change reaction. The same may be true for the Marquis reagent as well.
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QM33
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Registered: 04/09/20
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Re: DIY Psilocybin Content Percentage Detection [Re: joze]
#27770678 - 05/10/22 05:01 AM (1 year, 8 months ago) |
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Okay I must have missed something.
Why is the marquis test even an option? Is Marquis and mecke for md(m)a?
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