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OfflineSpectreOfCommunism
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Re: DIY Psilocybin Content Percentage Detection [Re: nektar61] * 1
    #27757822 - 04/30/22 04:16 PM (1 year, 8 months ago)

Quote:

nektar61 said:
Keep in mind that ground shrooms degrade psilocin with time, especially if in solution. Might want to make a fresh batch of shroom material each day of testing. That will better simulate  the way it will be used anyway.



Not sure where you’re getting your info on dry mushroom powder degrading with time — I see no reason why powdered dried mushrooms would have any difference in shelf-life from non-powdered dried mushrooms. You’re correct about wet mushrooms or wet mushroom powder — those will be inactive before long.

I explained earlier in this thread why it’s necessary to prepare a large batch of mushroom powder:
Quote:

One possibility (and the one I'm currently favoring) is to use the statistical principle of large numbers -- say I take a few ounces of dried samples grown from multispore inoculates of multiple varieties and grind it all to a uniformly mixed powder. Because this powder is a randomized mixture composed of thousands of individual fruits representing thousands of cubensis strains, we can be all but certain that samples taken therefrom will be very close to the average potency -- and if our analysis shows the same results after multiple small samples (e.g. 150mg), we can be 99.99...% certain.

So that procedure will give us the middle or average potency and that alone would be enough of a starting point to create a scale of "low, middle, high" potency through repeated trials.




In short, the whole purpose of powdering several ounces of genetically diverse cubensis material at once is to create a sample of very precisely average potency without which all these experiments would proceed by groping along in the dark. So grinding small samples each day on the scale of milligrams would not just be more tedious but also rob the experiments of their value altogether


--------------------
Found Species: Ps. Azurescens, Ps. Cyanescens, Ps. Baeocystis, Ps. Semilanceata, Pan. Cinctulus



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Offlinenektar61S
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Re: DIY Psilocybin Content Percentage Detection [Re: SpectreOfCommunism] * 1
    #27757844 - 04/30/22 04:32 PM (1 year, 8 months ago)

Quote:

SpectreOfCommunism said:
I explained earlier in this thread why it’s necessary to prepare a large batch of mushroom powder:
.... One possibility (and the one I'm currently favoring) is to use the statistical principle of large numbers -- say I take a few ounces of dried samples grown from multispore inoculates of multiple varieties and grind it all to a uniformly mixed powder.




I'd just use a clone to keep more even consistency.

FWIW, my comments here aren't directed at only you, but are more thinking out loud with the readers of the thread. I'm spitballing for anyone doing this, and am taking notes with what people bring to it.

As for grinding reducing potency, I don't have proof of this, I don't have a test for potency...(that's why I'm on this thread)

But I've heard over and over that grinding increases surface area which increases oxidation which increases psilocin in shrooms degrading to non-actives.

Interesting aside (and maybe somehow a hint toward finding the perfect DIY test, and maybe speaking to my thought of making a fresh sample each time), the bluing in bruised shrooms involves as you said, (an enzyme) turning psilocybin into psilocin, and another enzyme turning psilocin into a bluing...but the bluing is a longer chain that is close chemically to the chemical in Indigo dye that produces the bright blue.

If you make an ethanol (Everclear) extract of powdered shrooms, then a water extract, mix them, let it dehydrate a bit with a fan on it, it is BRIGHT blue, almost magical looking. For a little while. Within an hour or two, it's brown, and the potency goes down. I've done it.

Would be a nice party trick to do that and serve the blue liquid, maybe from a chalice if someone were into that. But even in paper cups, it's stunning looking.

I wonder if the test we seek is inbuilt...maybe making a slurry of shrooms and comparing the immediate blue is the answer, or part of the answer. Maybe especially if the shrooms are fresh. (Though a test needs to be able to test dried shrooms.)

Or maybe the proper test would use something that would imitate the enzymes in the shrooms, but have more of them than shrooms have. 


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Edited by nektar61 (04/30/22 05:03 PM)


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OfflineSpectreOfCommunism
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Re: DIY Psilocybin Content Percentage Detection [Re: nektar61] * 1
    #27757878 - 04/30/22 04:57 PM (1 year, 8 months ago)

Quote:

nektar61 said:
Quote:

SpectreOfCommunism said:
I explained earlier in this thread why it’s necessary to prepare a large batch of mushroom powder:
.... One possibility (and the one I'm currently favoring) is to use the statistical principle of large numbers -- say I take a few ounces of dried samples grown from multispore inoculates of multiple varieties and grind it all to a uniformly mixed powder.




I'd just use a clone to keep consistency more even.

As for grinding reducing potency, I don't have proof of this, I don't have a test for potency...(that's why I'm on this thread)

But I've heard over and over that grinding increases surface area which increases oxidation which increases psilocin in shrooms degrading to non-actives.

Interesting aside (and maybe somehow a hint toward finding the perfect DIY test, and maybe speaking to my thought of making a fresh sample each time), the bluing in bruised shrooms involves as you said, (an enzyme) turning psilocybin into psilocin, and another enzyme turning psilocin into a bluing...but the bluing is a longer chain that is close chemically to the chemical in Indago that produces the bright blue.

If you make an ethanol (Everclear) extract of powdered shrooms, then a water extract, mix them, let it dehydrate a bit with a fan on it, it is BRIGHT blue, almost magical looking. For a few hours. within 8 hours it's brown, and the potency goes down. I've done it.

Would be a nice party trick to do that and serve the blue liquid, maybe from a chalice if someone were into that. But even in paper cups, it's stunning looking.



Yes, in any aqueous solution, psilocybin will be rapidly converted to psilocin which is very unstable and will oxidize your to pretty blue inactive substance — but again, that requires water and there is no more water in a powdered dried mushroom than in a non-powdered dried mushroom. The enzymatic dephosphorylation of psilocybin requires water to occur which is why we dry our mushrooms as fast as possible — the faster you dry them, the fewer actives are converted to inactives.

Anyway, i’d be happy to take a look at any evidence to the contrary but, as someone who has been hunting and growing and eating these mushrooms for decades, I can tell you with 100% certainty that I have never seen any evidence that powder dried mushrooms are any less potent than non-powdered dried mushrooms. And from a chemistry perspective, I can’t think of any reason why psilocybin co tent would be impacted by grinding the dried material. I mean think about it – the mortar and pestle is basically the symbol of herbalism and alchemy throughout history. If grinding up herbs and mushrooms harmed the medicines locked in their cells, then I would think the mortar and pestle would be fairly anathema within the chemical sciences by now

And concerning the clone idea, yes that would be consistent but here would be no way to know how potent it was relative to the average potency. The measurements would be consistent but they would only be consistent relative to themselves and not to the overall range of potencies observable in cubensis — we’d be right back where we started, with unknown (albeit consistently unknown) sample potencies. With the statistical approach of using large sample sizes, we can be certain that we are measuring average samples which gives us a starting point that we can use to construct other non-average samples


--------------------
Found Species: Ps. Azurescens, Ps. Cyanescens, Ps. Baeocystis, Ps. Semilanceata, Pan. Cinctulus



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InvisibleIcyurmt
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Re: DIY Psilocybin Content Percentage Detection [Re: SpectreOfCommunism] * 2
    #27757881 - 04/30/22 04:59 PM (1 year, 8 months ago)


The chart above is for mushroom powder; unless it is well protected from oxygen and light, it can lose a lot of potency very quickly. This is the study thats from, it looks at tryptamine loss in mushrooms under various conditions as well as the effects of drying.
PDF Stability of psilocybin and its four analogs in the biomass of the psychotropic mushroom Psilocybe cubensis


--------------------
👁️ 🌊 why you are empty.

Hunt for the habitat not the mushroom.


Edited by Icyurmt (04/30/22 05:09 PM)


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Offlinenektar61S
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Re: DIY Psilocybin Content Percentage Detection [Re: Icyurmt] * 1
    #27757898 - 04/30/22 05:11 PM (1 year, 8 months ago)

Quote:

Icyurmt said:
The chart above is for mushroom powder; unless it is well protected from oxygen and light, it can lose a lot of potency very quickly.




Well there we go.

It's possible that SpectreOfCommunism's contrary results can be human vs chemical (makes sense, if human brain was a good test, we wouldn't need a chemical test.)

Or SpectreOfCommunism's shrooms had a lot of psilocybin and not much psilocin, so grinding didn't degrade much.

--
All:

According to:
https://www.swgdrug.org/Monographs/PSILOCIN.pdf
(use a VPN, that's a cop site, "Scientific Working Group for the Analysis of Seized Drugs" haha):

Psilocin is only slightly soluble in water.
Psilocybin is soluble in water.

and
Psilocin is soluble in methanol.
Psilocybin is only slightly soluble in methanol.

So maybe methanol isn't a good solution to use. Or am I missing something and the acid takes care of that or something with commercial tests and with what SpectreOfCommunism is trying? This thread is getting harder to navigate, which is good, means it's getting a lot of response. I really think this is a "throw the crowd at it" problem.

It's weird that most commercial tests and cop tests seem to be using methanol, but that might mean the mainly end up only testing the Psilocin and are not even testing much of the Psilocybin in a given sample.

I remember the reason I used ethanol and then water for my "magic blue chalice liquid" was somewhere I found that Psilocin dissolves well in one and Psilocybin dissolves well in the other.

There is some info in that link above about color change results for yet more reagents with Psilocybin and Psilocin that might help with all this:



I'm thinking maybe the PsiloQ test may be mostly Marquis reagent (simple to make: "a mixture of formaldehyde and concentrated sulfuric acid, which is dripped onto the substance being tested.")
https://en.wikipedia.org/wiki/Marquis_reagent

It turns greenish-brown for Psilocin, and dull orange for Psilocybin. Both together would likely be brown

"The United States Department of Justice method for producing Marquis reagent is the addition of 100 mL of concentrated (95–98%) sulfuric acid to 5 mL of 40% formaldehyde.[1]: 12  Different compounds produce different color reactions. Methanol may be added to slow down the reaction process to allow better observation of the colour change."

One advantage we have for the test we're trying to make is it only has to test for two things. It doesn't have to test for adulterants or other drugs like a street drug test.



--------------------
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Edited by nektar61 (04/30/22 05:31 PM)


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OfflineSpectreOfCommunism
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Re: DIY Psilocybin Content Percentage Detection [Re: nektar61] * 3
    #27757940 - 04/30/22 05:43 PM (1 year, 8 months ago)

Methinks you guys are misinterpreting that study. From the study:
Quote:

“We found that the dried fungal fruiting bodies had a better yield from a fungal powder than from whole pieces. Conversely, in the case of fresh fungal fruiting bodies, the extraction of larger unprocessed pieces of fresh fruiting bodies was found to be more effective than chopping these mushrooms into small pieces”



The researchers say that for dried specimens they got “a better yield from a fungal powder than from whole pieces” and that the opposite was the case for fresh (i.e. water-containing) mushrooms, which agrees exactly with what I’ve said.


--------------------
Found Species: Ps. Azurescens, Ps. Cyanescens, Ps. Baeocystis, Ps. Semilanceata, Pan. Cinctulus



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OfflineSpectreOfCommunism
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Re: DIY Psilocybin Content Percentage Detection [Re: SpectreOfCommunism] * 1
    #27757986 - 04/30/22 06:34 PM (1 year, 8 months ago)

Also from that study:
Quote:

In summary, the yields of the analytes from the dried mushroom powder in comparison with the unprocessed dried mushrooms were as follows: 16% increase in psilocybin, 13% increase in psilocin, 74% increase in baeocystin, and 40% increase in aeruginascin.




And as for the degradation of  tryptamines in powder form, they’re talking about this degradation over the course of 15 months, particularly when left in the light in the presence of oxygen. Which, yeah, that’s a silly way to store them.


--------------------
Found Species: Ps. Azurescens, Ps. Cyanescens, Ps. Baeocystis, Ps. Semilanceata, Pan. Cinctulus



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InvisibleIcyurmt
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Re: DIY Psilocybin Content Percentage Detection [Re: SpectreOfCommunism] * 2
    #27758027 - 04/30/22 07:30 PM (1 year, 8 months ago)

Quote:

Methinks you guys are misinterpreting that study. From the study:
Quote:



Quote:

The researchers say that for dried specimens they got “a better yield from a fungal powder than from whole pieces” and that the opposite was the case for fresh (i.e. water-containing) mushrooms, which agrees exactly with what I’ve said.




That's from the section 3.1 that was looking specifically at extraction efficiency, not storage effects. That says that it's more efficient to extract from powdered than it is from whole dry pieces, which I don't think anybody was disagreeing with. The storage of fresh mushrooms is covered in section 3.6.

Quote:

And as for the degradation of tryptamines in powder form, they’re talking about this degradation over the course of 15 months, particularly when left in the light in the presence of oxygen.



And as shown on the chart above and in section 3.7, approximately half the initial potency was lost within only one month when the powered mushrooms were stored, even for those in a bag in the dark.
]
From the conclusion.


--------------------
👁️ 🌊 why you are empty.

Hunt for the habitat not the mushroom.


Edited by Icyurmt (04/30/22 07:36 PM)


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OfflineSpectreOfCommunism
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Re: DIY Psilocybin Content Percentage Detection [Re: Icyurmt] * 1
    #27758067 - 04/30/22 08:17 PM (1 year, 8 months ago)

Either way, it’s an interesting paper — it’s unfortunate that they don’t include a comparison of potency loss over time between whole dried mushrooms and powdered dried mushrooms. Like, I recognize that powder dried mushrooms tend to lose potency over time just like every other form of mushrooms but drastic potency loss after more than a year seems to be normal compared to whole dried mushrooms. A few searches on here and on the internet at large seems to indicate that the general consensus is that properly dried and stored mushrooms will last about a year — although my own experience is that they last longer. But I vacuum pack mine, so maybe the lack of oxygen in the packaging has served me well idk — I know I’ve eaten three year old cyanescens that seemed as potent as the day I dried them :shrug:


--------------------
Found Species: Ps. Azurescens, Ps. Cyanescens, Ps. Baeocystis, Ps. Semilanceata, Pan. Cinctulus



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Offlinenektar61S
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Re: DIY Psilocybin Content Percentage Detection [Re: Icyurmt] * 2
    #27758075 - 04/30/22 08:25 PM (1 year, 8 months ago)

Quote:

Icyurmt said:
And as shown on the chart above and in section 3.7, approximately half the initial potency was lost within only one month when the powered mushrooms were stored, even for those in a bag in the dark.




right.

There is some first-hand (non-scientific) info on Shroomery of someone storing non-ground shrooms in a hot garage for 10 years and still having them be very strong.

Forrester's quote here, in the vacuum pack and long-term storage thread:
https://www.shroomery.org/forums/showflat.php/Number/27660424#27660424

Seems to imply that a warm garage is less damaging to potency than air is. I'll ask him to clarify. (He did not vacuum pack, he stored in a jar, but did not grind the shrooms.)

I've probably seen 30 people on here recommend against grinding shrooms for storage....all say it degrades potency quicker. 

I'm going to do my testing using a clone (x7x plin clone I use for everything, it's the main thing I grow now). And only stems or only caps, since stems are supposed to be a little stronger than caps. I'll leave it un-ground until I test.

I think clones make sense over grinding, not just because grinding degrades, but because we're testing tiny amounts, and even a ground mix of wildly varying potency ms shrooms could have noticeably different amounts of actives a few cm apart in the same bag.
--
Quote:

SpectreOfCommunism said:
although my own experience is that they last longer. But I vacuum pack mine, so maybe the lack of oxygen in the packaging has served me well idk.. :shrug:




Yes. I also vacuum pack and have started a ten-year study (with clones). Vacuum packing is about the opposite of grinding.

Think about activated charcoal. It's ground fine to increase surface area, to increase absorption. When you grind shrooms you're vastly increasing the amount of surface area.
=--
Intersting that article mentions shrooms losing potency in freezer. But they meniton -80 c. Who the fuck has a freezer that goes to that (-112 f). I guess labs do.

I store live cultures in a dedicated dorm fridge for 6 plus months (and counting) at 2 c (about 35 f), I assume you could store dried shrooms at that.

I think the problem with freezing or even cold is that you draw water in.

I'm intrigued by the "inert gas" comment, I was thinking that the other day and wondering about buying a tank of argon. Is about 300 bucks.

There isn't a lot of info on long term storage of shrooms, but from what I've heard, best bet is maybe vacuum sealed, in the dark, at room temp.


--------------------
-NEW? Start here.


Edited by nektar61 (04/30/22 09:10 PM)


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InvisibleIcyurmt
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Re: DIY Psilocybin Content Percentage Detection [Re: nektar61] * 1
    #27758118 - 04/30/22 09:06 PM (1 year, 8 months ago)

Quote:

it’s unfortunate that they don’t include a comparison of potency loss over time between whole dried mushrooms and powdered dried mushrooms.



They did look at mostly whole mushrooms stored for 3 months in the dark at room temp in section 3.6. I believe. Those apparently showed little to no loss during that time.

I think you would probably still want to grind/homogenize before testing with clones just because of the variation that can occur in different parts like cap and stripe. Doing so closer to the time of testing though would make the most sense imo.

Quote:

I'm intrigued by the "inert gas" comment, I was thinking that the other day and wondering about buying a tank of argon. Is about 300 bucks.



I think either buying of making something like this purgeable storage box, out of an otterbox or pelican case, would be the most ideal for long-term storage. Tig welders will often have argon, so if you know anyone who welds you can probably convince them to fill up a few balloons for you.


--------------------
👁️ 🌊 why you are empty.

Hunt for the habitat not the mushroom.


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OfflineSpectreOfCommunism
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Re: DIY Psilocybin Content Percentage Detection [Re: nektar61] * 2
    #27758122 - 04/30/22 09:09 PM (1 year, 8 months ago)

Nektar, what are you going to compare your clone material to? How do you get from “reagent plus mushroom results in color-reaction” to something you can quantify? My whole point with the grinding of many, many different mushrooms with different genetics is that, by doing so, probability theory and the law of large numbers guarantees that such a sample will contain the mathematical average quantity of alkaloids. This then allows us to extrapolate that 1/2 of a given sample will give us mushroom material that is 0.5x average potency, 2/1 of the same sample will give us mushroom material that is 2.0x the average potency, etc and so on, which gives us a way to devise a quantitative scale that can be re-used.


--------------------
Found Species: Ps. Azurescens, Ps. Cyanescens, Ps. Baeocystis, Ps. Semilanceata, Pan. Cinctulus



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OfflineSpectreOfCommunism
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Re: DIY Psilocybin Content Percentage Detection [Re: SpectreOfCommunism] * 1
    #27758129 - 04/30/22 09:17 PM (1 year, 8 months ago)

Icyurmt, 3.6 deals with “fresh fruiting bodies” not whole dried mushrooms. I’ve read the thing a few times now and I can’t find any part where they compare potency loss between whole dried and powdered dried mushrooms. Additionally, all the comparison tables seem to indicate that they’re only comparing the storage of dry powder and fresh fruiting bodies under various temperature and lighting conditions


--------------------
Found Species: Ps. Azurescens, Ps. Cyanescens, Ps. Baeocystis, Ps. Semilanceata, Pan. Cinctulus



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Offlinenektar61S
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Re: DIY Psilocybin Content Percentage Detection [Re: SpectreOfCommunism] * 1
    #27758136 - 04/30/22 09:25 PM (1 year, 8 months ago)

Quote:

SpectreOfCommunism said:
Nektar, what are you going to compare your clone material to? How do you get from “reagent plus mushroom results in color-reaction” to something you can quantify? My whole point with the grinding of many, many different mushrooms with different genetics is that, by doing so, probability theory and the law of large numbers guarantees that such a sample will contain the mathematical average quantity of alkaloids. This then allows us to extrapolate that 1/2 of a given sample will give us mushroom material that is 0.5x average potency, 2/1 of the same sample will give us mushroom material that is 2.0x the average potency, etc and so on, which gives us a way to devise a quantitative scale that can be re-used.




A lab would order a small known sample of each chemical. We can't do that, so....

I haven't gotten to that point yet. I've ordered a couple reagents. I will test using a dried clone harvested in the past month or two, to try to get rough quantitative color variation results, then test again to confirm it's repeatable.

I'd consider it a win if I can get a different visible color comparison result between testing 150 mg and testing 300 mg  dried of the same clone. Something that does that consistently would allow a profile to be formed. (unless doing that throws off the test and gives different results from the same amount of two samples where one is twice the potency.)

If I get really into it, maybe I'll send a sample of it off to a lab (like the one A-Rock) runs, pay the 100 dollars+ to find the actual percent quantity of that. That could get expensive quick though. I still don't think you'd have to test 6 or 7, I think you could test 1, and use 6 or 7 varying amounts of that shroom to test. If you knew the percent in the clone, that might work.

Or maybe ask some people in the Psilo Cup to send samples of their clones, since those have been tested with HPLC and the amounts are known.

So far I will just be looking for something that gives a reasonable color difference between say, a tiny bit, a little bit, a bit, and a lot. From there it can be fine tuned.

How do we know if the PsiloQ / Miraculix test even did the last step of taking the color chart they made, having HPLC lab tests on all the samples, and putting the correct percentages on the color chart?

I'm not saying they're scammy, it looks legit, but I think the calibration could be done with one shroom clone sample that has gone to a lab for tests.

They don't claim on the site that they tested, they only claim within 10% accuracy of HPLC lab tests. If I made and sold that test, and had done the lab tests to prove that amount, I'd provide the data in the marketing materials. Again, not saying they didn't, but why not?

Though maybe they did the tests, since Alan called the guy who runs it by his first name on here. haha. If you're on a first-name basis with A-Rock, you might just run a lab.

I'd be happy to just be able to tell weak from strong from super strong shrooms at home. That would be useful to me. And I bet to a lot of other people.

I'm using a clone because it makes sense. Using a mix of ground shrooms doesn't make sense to me. And I have seen enough people on this site post about doing an "experiment" then getting shot down by TCs (with good reason) because they didn't use a clone. Search "experiment" in advanced search and read the results.

I'm mainly interested in this for me to have the test. But I will share any of my results with the community. I don't want to produce data that the community will dismiss outright.
--
Here's some of the info from what they claim, might be useful to review for anyone interested in working on a test:

Quote:

This test is performed in under 1/2 hour, and requires only 150mg of starting material.

--Fast evaluation after 30 minutes.
--Detection range between 0.1 - 2 % active ingredient content.
--Only 150 mg starting material is required.
--10 % deviation from HPLC analysis.
--Evaluation of the test kits with the naked eye and evaluation scale.
--The PSILO-QTest for concentration determination is a single-use test.

INSTRUCTIONS

--Preparation: Crush dried mushroom material. Weigh out approx 150 mg of starting material.
--Extraction: Add weighed starting material to the supplied extraction liquid (yellowish liquid in translucent plastic bottle)
--Detection: Use the sterile syringe/needle to take up extraction solution, and add it to the detection solution (the clear liquid in glass bottle)
--Evaluation: Allow color complex to develop (over about 1/2 hour) and evaluate with evaluation scale.




--------------------
-NEW? Start here.


Edited by nektar61 (04/30/22 10:12 PM)


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InvisibleIcyurmt
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Re: DIY Psilocybin Content Percentage Detection [Re: nektar61] * 1
    #27758150 - 04/30/22 09:53 PM (1 year, 8 months ago)

Quote:

SpectreOfCommunism said:
Icyurmt, 3.6 deals with “fresh fruiting bodies” not whole dried mushrooms. I’ve read the thing a few times now and I can’t find any part where they compare potency loss between whole dried and powdered dried mushrooms. Additionally, all the comparison tables seem to indicate that they’re only comparing the storage of dry powder and fresh fruiting bodies under various temperature and lighting conditions



Section 3.6 was looking for the optimal drying and storage conditions to maintain tryptamine content. The fresh fruiting bodies in group 2, were left at room temperature in the dark for 3 months (taken along with the comment in that section about the confirmation of drying not reduce the tryptamine content, I believe that implies they were allowed to naturally dry, as fresh fruits would not last 3 months without doing so on their own).

All of the comparison charts they give appear to be specific to each section they are from. The study does not do a direct comparison with the samples from section 3.6 and the dried fungal powder in 3.7, but they do report on the various concentrations/% found, so it's possible to compare those results with the concentrations/losses found with the powder from section 3.7.


--------------------
👁️ 🌊 why you are empty.

Hunt for the habitat not the mushroom.


Edited by Icyurmt (04/30/22 10:38 PM)


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Re: DIY Psilocybin Content Percentage Detection [Re: shr00med] * 2
    #27758175 - 04/30/22 10:37 PM (1 year, 8 months ago)

If the PsiloQ test were a lot cheaper, I'd just buy it and wouldn't even be on this thread.

15 bucks for a one-use visual test with 90% accuracy seems expensive. It's probably 1 or 2 reagents mixed, maybe with some other common chemical. I'd wager it's a lot cheaper to make.

If it were the price of Reagents, like 30 bucks for 20 tests, (where the sellers still make a profit), I'd just buy it.

Kudos to the guy for cracking the code, but I think he's overpricing it.

Though with the Shroom-Boom ramping up I'm sure there are yuppies who'd buy crates full at his price.


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-NEW? Start here.


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InvisibleIcyurmt
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Re: DIY Psilocybin Content Percentage Detection [Re: nektar61] * 1
    #27758194 - 04/30/22 10:58 PM (1 year, 8 months ago)

If not marquis perhaps it's mecke? Does anyone know what color mecke reagent turns when reacted with psilocybin/psilocin? I can't find any info when I looked but when reacting with DMT and lsd it looks very similar to the PsiloQ tests color. I know mecke also reacts with sugars so you would likely want to use an extract solvent that accounted for that if possible. Anyone know how soluble psilocybin/psilocin is in something like hexane or perhaps something better?


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👁️ 🌊 why you are empty.

Hunt for the habitat not the mushroom.


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OfflineSpectreOfCommunism
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Re: DIY Psilocybin Content Percentage Detection [Re: Icyurmt] * 1
    #27758198 - 04/30/22 11:00 PM (1 year, 8 months ago)

Quote:

Icyurmt said:
Quote:

SpectreOfCommunism said:
Icyurmt, 3.6 deals with “fresh fruiting bodies” not whole dried mushrooms. I’ve read the thing a few times now and I can’t find any part where they compare potency loss between whole dried and powdered dried mushrooms. Additionally, all the comparison tables seem to indicate that they’re only comparing the storage of dry powder and fresh fruiting bodies under various temperature and lighting conditions



Section 3.6 was looking for the optimal drying and storage conditions to maintain tryptamine content. The fresh fruiting bodies in group 2, were left at room temperature in the dark for 3 months (taken along with the comment in that section, I believe that implies naturally dried, as fresh fruits would not last 3 months without doing so).

All of the comparison charts they give appear to be specific to each section they are from. The study does not do a direct comparison with the samples from section 3.6 and the dried fungal powder in 3.7, but they do report on the various concentrations/% found, so it's possible to compare those results with the concentrations/losses found with the powder from section 3.7.




I dunno, I'm not seeing/reading anything in there to suggest that they are comparing dried to dried and powdered mushrooms -- in the conclusion, they only mention dried-powdered versus fresh and, in all the comparison tables, they indicate dried-powdered versus fresh. There are a few passages where the wording is vague and so, out of context, it seems like they could be referring to dried whole mushrooms -- but it would be really weird if they left out 1/3 of the subjects they're comparing in all the tables and then go on to not even mention the 3rd thing being compared in the conclusion.

Unless they're referring to dried mushrooms as "fresh mushrooms" idk -- but then up is down and left is right and words have no meaning lol


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Found Species: Ps. Azurescens, Ps. Cyanescens, Ps. Baeocystis, Ps. Semilanceata, Pan. Cinctulus



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OfflineSpectreOfCommunism
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Re: DIY Psilocybin Content Percentage Detection [Re: Icyurmt] * 2
    #27758212 - 04/30/22 11:17 PM (1 year, 8 months ago)

Quote:

Icyurmt said:
If not marquis perhaps it's mecke? Does anyone know what color mecke reagent turns when reacted with psilocybin/psilocin? I can't find any info when I looked but when reacting with DMT and lsd it looks very similar to the PsiloQ tests color. I know mecke also reacts with sugars so you would likely want to use an extract solvent that accounted for that if possible. Anyone know how soluble psilocybin/psilocin is in something like hexane or perhaps something better?



Mecke is actually my best guess for what Miraculix is doing, as well -- but I also can't find any info on Mecke and psilocybin. However, because it's reaction with other indoles like DMT seems to fit the color profile, it seems worth investigating


--------------------
Found Species: Ps. Azurescens, Ps. Cyanescens, Ps. Baeocystis, Ps. Semilanceata, Pan. Cinctulus



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InvisibleIcyurmt
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Re: DIY Psilocybin Content Percentage Detection [Re: SpectreOfCommunism] * 1
    #27758233 - 04/30/22 11:43 PM (1 year, 8 months ago)

Quote:

Unless they're referring to dried mushrooms as "fresh mushrooms" idk -- but then up is down and left is right and words have no meaning lol



I think fresh mushrooms left out at room temperature for 3 months would soon become dried mushrooms all on their own, they all start out fresh and drying was a part of the "processing" they were looking at with the tests in that section. It also helps explain why they cite that test of group 2, as further support for their conclusion that drying in the dark at room temp does not cause a reduction of tryptamine content. The "preparation" of the fresh mushrooms is described at the beginning of that section and in section 2.2.

They report on the %/levels of various tryptamines found in those samples after 3 months of storage. The tests on the power did not look specifically after 3 months but instead after 1week, 1month, 2months, and 15months(different sections so different set of tests). Even so, a comparison of the levels found after one month as power vs 3 months mostly whole in the dark, can still be done from the data they provide.


--------------------
👁️ 🌊 why you are empty.

Hunt for the habitat not the mushroom.


Edited by Icyurmt (04/30/22 11:58 PM)


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