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Invisiblejoze
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Loc: PNW
Re: DIY Psilocybin Content Percentage Detection [Re: shr00med] * 1
    #27716466 - 04/01/22 12:12 PM (1 year, 9 months ago)

I'm also curious: what are y'all planning on using as a reference? The Miraculix kit has a scale they've developed themselves with their specific reagent. You're talking about determining the potency of the mushrooms, which is essentially the same as talking about the concentration of psilocybin/psilocin. Okay, so you can test your mushrooms with this reagent and measure the color, but what are you going to compare the value to? Unless you are able to source a relatively pure sample of psilocybin or psilocin in a known concentration, you will be limited to comparing relative potencies of mushrooms rather than measuring the actual concentrations.


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Edited by joze (04/01/22 12:13 PM)


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InvisibleQM33
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Registered: 04/09/20
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Re: DIY Psilocybin Content Percentage Detection [Re: joze] * 1
    #27716484 - 04/01/22 12:20 PM (1 year, 9 months ago)

But I mean shit, that could have its own value in a way if it was significantly cheaper or quicker maybe..?


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Invisiblejoze
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Loc: PNW
Re: DIY Psilocybin Content Percentage Detection [Re: QM33] * 1
    #27716496 - 04/01/22 12:30 PM (1 year, 9 months ago)

Quote:

QM33 said:
But I mean shit, that could have its own value in a way if it was significantly cheaper or quicker maybe..?



Oh, I absolutely agree. I just think it's important for everyone to be aware of what they're actually measuring.

A quick, inexpensive method to compare relative potency of different cultures would be borderline revolutionary for the hobby grower. Being able to say okay, these golden teachers are more potent than the B+ I grew, that would be awesome. But at the same time, it would be difficult to compare potencies with other growers if you don't have that true measurement of potency.


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InvisibleQM33
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Registered: 04/09/20
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Loc: Oregon
Re: DIY Psilocybin Content Percentage Detection [Re: joze] * 1
    #27716502 - 04/01/22 12:33 PM (1 year, 9 months ago)

Totally, but you might have about all you need to guarantee your isolating you most potent genetics, and maybe once you got to a certain point you could take it for further analysis, rather than testing multiple genetics with more costly analysis...

What's going on here?! I want some


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Invisiblejoze
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Re: DIY Psilocybin Content Percentage Detection [Re: shr00med] * 1
    #27716506 - 04/01/22 12:36 PM (1 year, 9 months ago)

Agreed! Looking forward to seeing where this thread goes!

:robindance:


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OfflineSpectreOfCommunism
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Re: DIY Psilocybin Content Percentage Detection [Re: joze] * 3
    #27717225 - 04/01/22 10:42 PM (1 year, 9 months ago)

Quote:

joze said:
I'm also curious: what are y'all planning on using as a reference? The Miraculix kit has a scale they've developed themselves with their specific reagent. You're talking about determining the potency of the mushrooms, which is essentially the same as talking about the concentration of psilocybin/psilocin. Okay, so you can test your mushrooms with this reagent and measure the color, but what are you going to compare the value to? Unless you are able to source a relatively pure sample of psilocybin or psilocin in a known concentration, you will be limited to comparing relative potencies of mushrooms rather than measuring the actual concentrations.




Firstly, I'd say -- for the purposes of most of us anyway -- even being able to consistently tell whether this sample is relatively low-potency, this one average, and this one high-potency would be a pretty heckin' useful test.

And secondly, concerning a reference, I can think of a few approaches that don't involve already having any known quantities of psilocybin.

One possibility (and the one I'm currently favoring) is to use the statistical principle of large numbers -- say I take a few ounces of dried samples grown from multispore inoculates of multiple varieties and grind it all to a uniformly mixed powder. Because this powder is a randomized mixture composed of thousands of individual fruits representing thousands of cubensis strains, we can be all but certain that samples taken therefrom will be very close to the average potency -- and if our analysis shows the same results after multiple small samples (e.g. 150mg), we can be 99.99...% certain.

So that procedure will give us the middle or average potency and that alone would be enough of a starting point to create a scale of "low, middle, high" potency through repeated trials.

Another starting point would be to use the known molar masses of the substances in question -- in this case, psilocybin/psilocin and p-DMAB -- and the fact that the reagent's color-reaction is the result of each molecule of p-DMAB binding to two indole molecules (psilocybin/psilocin) to work out the upper and lower limits of the reaction with given weights of both materials.

I think the statistical approach will be more practical -- but we'll see :smile: Currently I have p-DMAB, as well as a few different acids to make different forms of the reagent, but I am waiting on an online order of phosphoric acid because my research leads me to believe that using phosphoric acid and methanol will give me the most sensitive reagent to work with. Once that arrives, I plan to proceed along the statistical approach I outlined above.


--------------------
Found Species: Ps. Azurescens, Ps. Cyanescens, Ps. Baeocystis, Ps. Semilanceata, Pan. Cinctulus



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Invisiblejoze
Male


Registered: 11/10/20
Posts: 928
Loc: PNW
Re: DIY Psilocybin Content Percentage Detection [Re: SpectreOfCommunism] * 1
    #27717686 - 04/02/22 09:46 AM (1 year, 9 months ago)

Quote:

SpectreOfCommunism said:
Quote:

joze said:
I'm also curious: what are y'all planning on using as a reference? The Miraculix kit has a scale they've developed themselves with their specific reagent. You're talking about determining the potency of the mushrooms, which is essentially the same as talking about the concentration of psilocybin/psilocin. Okay, so you can test your mushrooms with this reagent and measure the color, but what are you going to compare the value to? Unless you are able to source a relatively pure sample of psilocybin or psilocin in a known concentration, you will be limited to comparing relative potencies of mushrooms rather than measuring the actual concentrations.




Firstly, I'd say -- for the purposes of most of us anyway -- even being able to consistently tell whether this sample is relatively low-potency, this one average, and this one high-potency would be a pretty heckin' useful test.

And secondly, concerning a reference, I can think of a few approaches that don't involve already having any known quantities of psilocybin.

One possibility (and the one I'm currently favoring) is to use the statistical principle of large numbers -- say I take a few ounces of dried samples grown from multispore inoculates of multiple varieties and grind it all to a uniformly mixed powder. Because this powder is a randomized mixture composed of thousands of individual fruits representing thousands of cubensis strains, we can be all but certain that samples taken therefrom will be very close to the average potency -- and if our analysis shows the same results after multiple small samples (e.g. 150mg), we can be 99.99...% certain.

So that procedure will give us the middle or average potency and that alone would be enough of a starting point to create a scale of "low, middle, high" potency through repeated trials.

Another starting point would be to use the known molar masses of the substances in question -- in this case, psilocybin/psilocin and p-DMAB -- and the fact that the reagent's color-reaction is the result of each molecule of p-DMAB binding to two indole molecules (psilocybin/psilocin) to work out the upper and lower limits of the reaction with given weights of both materials.

I think the statistical approach will be more practical -- but we'll see :smile: Currently I have p-DMAB, as well as a few different acids to make different forms of the reagent, but I am waiting on an online order of phosphoric acid because my research leads me to believe that using phosphoric acid and methanol will give me the most sensitive reagent to work with. Once that arrives, I plan to proceed along the statistical approach I outlined above.



That would be a good method of defining your average potency and then testing cultures to see whether they are above-average or below-average. I'm curious to see how your trials turn out. Another question, how are you all planning to measure your data? Do you have a colorimeter or something? Or are you just eyeballing shit?


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Invisibleshr00med
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Re: DIY Psilocybin Content Percentage Detection [Re: joze] * 1
    #27721860 - 04/05/22 08:38 AM (1 year, 9 months ago)

I was planning on eyeballing it, definitely using my camera as well. Reagents tend to change color and/or darken with time, so timing the reaction would be essential. Colorimeter would be ideal, but this *future* tek is targeted for those with limited resources.
Also- I don't see why you couldn't take a sample with an averaged, known value (for example Pan Cyanenscens has 2.5% psilocybin and 1.194% psilocin so ~3.69% [with ~10% variability] alkaloid content) and test the same fruit yourself, recording the color.


Edited by shr00med (04/05/22 08:49 AM)


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Invisiblejoze
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Re: DIY Psilocybin Content Percentage Detection [Re: SpectreOfCommunism] * 2
    #27722055 - 04/05/22 12:12 PM (1 year, 9 months ago)

Quote:

shr00med said:
I was planning on eyeballing it, definitely using my camera as well. Reagents tend to change color and/or darken with time, so timing the reaction would be essential. Colorimeter would be ideal, but this *future* tek is targeted for those with limited resources.
Also- I don't see why you couldn't take a sample with an averaged, known value (for example Pan Cyanenscens has 2.5% psilocybin and 1.194% psilocin so ~3.69% [with ~10% variability] alkaloid content) and test the same fruit yourself, recording the color.



Do you not see the problem with what you're saying?

You're wanting to use this reagent to accurately measure alkaloid content, because psychedelic mushrooms can vary in their potency. But for your reference, you want to use a psychedelic mushroom, because you think you can assume its alkaloid content... If you're so confident that each species has <10% variability, then what's the point in testing?

I will go to my death bed arguing that if you want to use 1) a DIY reagent with slightly variable concentrations and 2) eyeball your results, you will need a better reference sample to obtain accurate results.

Quote:

SpectreOfCommunism said:
One possibility (and the one I'm currently favoring) is to use the statistical principle of large numbers -- say I take a few ounces of dried samples grown from multispore inoculates of multiple varieties and grind it all to a uniformly mixed powder. Because this powder is a randomized mixture composed of thousands of individual fruits representing thousands of cubensis strains, we can be all but certain that samples taken therefrom will be very close to the average potency -- and if our analysis shows the same results after multiple small samples (e.g. 150mg), we can be 99.99...% certain.



One possibility for obtaining a better reference actually comes from what SpectreofCommunism said above. If you made a homogenized batch of a bunch of powdered mushroom, you could then store that as a "reference sample" jar as long as you store it in a way that the alkaloids don't significantly degrade over time. If you got a Miraculix test kit (since we actually know these ARE precise), you could measure the alkaloid content of the reference sample with both Miraculix as well as your DIY reagent multiple times. If your DIY reagent gave you relatively consistent results, you could correlate that color change to the specific concentration that the Miraculix results gave you.


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OfflineAlan RockefellerM
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Re: DIY Psilocybin Content Percentage Detection [Re: shr00med] * 2
    #27722219 - 04/05/22 03:39 PM (1 year, 9 months ago)

Quote:

shr00med said:
Also- I don't see why you couldn't take a sample with an averaged, known value (for example Pan Cyanenscens has 2.5% psilocybin and 1.194% psilocin so ~3.69% [with ~10% variability] alkaloid content) and test the same fruit yourself, recording the color.





The content varies a whole lot more than that - every species varies by at least 50%, maybe more.


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OfflineSpectreOfCommunism
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Re: DIY Psilocybin Content Percentage Detection [Re: joze] * 1
    #27722515 - 04/05/22 08:00 PM (1 year, 9 months ago)

Quote:

joze said:
Quote:

SpectreOfCommunism said:
Quote:

joze said:
I'm also curious: what are y'all planning on using as a reference? The Miraculix kit has a scale they've developed themselves with their specific reagent. You're talking about determining the potency of the mushrooms, which is essentially the same as talking about the concentration of psilocybin/psilocin. Okay, so you can test your mushrooms with this reagent and measure the color, but what are you going to compare the value to? Unless you are able to source a relatively pure sample of psilocybin or psilocin in a known concentration, you will be limited to comparing relative potencies of mushrooms rather than measuring the actual concentrations.




Firstly, I'd say -- for the purposes of most of us anyway -- even being able to consistently tell whether this sample is relatively low-potency, this one average, and this one high-potency would be a pretty heckin' useful test.

And secondly, concerning a reference, I can think of a few approaches that don't involve already having any known quantities of psilocybin.

One possibility (and the one I'm currently favoring) is to use the statistical principle of large numbers -- say I take a few ounces of dried samples grown from multispore inoculates of multiple varieties and grind it all to a uniformly mixed powder. Because this powder is a randomized mixture composed of thousands of individual fruits representing thousands of cubensis strains, we can be all but certain that samples taken therefrom will be very close to the average potency -- and if our analysis shows the same results after multiple small samples (e.g. 150mg), we can be 99.99...% certain.

So that procedure will give us the middle or average potency and that alone would be enough of a starting point to create a scale of "low, middle, high" potency through repeated trials.

Another starting point would be to use the known molar masses of the substances in question -- in this case, psilocybin/psilocin and p-DMAB -- and the fact that the reagent's color-reaction is the result of each molecule of p-DMAB binding to two indole molecules (psilocybin/psilocin) to work out the upper and lower limits of the reaction with given weights of both materials.

I think the statistical approach will be more practical -- but we'll see :smile: Currently I have p-DMAB, as well as a few different acids to make different forms of the reagent, but I am waiting on an online order of phosphoric acid because my research leads me to believe that using phosphoric acid and methanol will give me the most sensitive reagent to work with. Once that arrives, I plan to proceed along the statistical approach I outlined above.



That would be a good method of defining your average potency and then testing cultures to see whether they are above-average or below-average. I'm curious to see how your trials turn out. Another question, how are you all planning to measure your data? Do you have a colorimeter or something? Or are you just eyeballing shit?



My hope is that, once all is said and done, my hypothetical test will be eyeball-able but, during my work of calibrating the test, I plan to use a combination of taking very consistent photos against the same background and photo-editing software that will allow me to sample the colors in a digitally consistent way in order to devise a colorimetric scale


--------------------
Found Species: Ps. Azurescens, Ps. Cyanescens, Ps. Baeocystis, Ps. Semilanceata, Pan. Cinctulus



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OfflineAlan RockefellerM
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Re: DIY Psilocybin Content Percentage Detection [Re: SpectreOfCommunism] * 2
    #27722571 - 04/05/22 08:46 PM (1 year, 9 months ago)

Quote:

SpectreOfCommunism said:
My hope is that, once all is said and done, my hypothetical test will be eyeball-able but, during my work of calibrating the test, I plan to use a combination of taking very consistent photos against the same background and photo-editing software that will allow me to sample the colors in a digitally consistent way in order to devise a colorimetric scale





Make sure you use manual mode on the camera, with the camera and sample in the exact same place and with the exact same lighting, otherwise the automatic exposure and auto white balance will affect the colors.


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OfflineSpectreOfCommunism
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Re: DIY Psilocybin Content Percentage Detection [Re: Alan Rockefeller] * 4
    #27753993 - 04/27/22 08:01 PM (1 year, 8 months ago)

I've got an update for you all :smile: I've officially begun experimenting to find a procedure like the one I've outlined earlier in this thread and my initial results are very promising. To recap for anyone who hasn't followed closely, my goal is to design a DIY colorimetric test that will allow the average Shroomerite to determine the potency of their magic mushrooms using a reagent (p-DMAB) and a color-scale much like those little pH test-strips people use for pools and fish-tanks.

Anyway, without further ado, here is a quick write-up of my procedure so far:

For my starting material, I prepared 78g of fine powder by grinding a gallon-sized ziplock bag of cracker-dry cubensis specimens representing hundreds (if not thousands) of different strains grown from MS syringes of 4 well-known varieties* under the same conditions on brown rice-flour substrate. As I've explained earlier in this thread, this procedure ensures that the starting material is very close to the overall average potency of cubensis.

For my reagent solution, I dissolved 5g of p-dimethylaminobenzaldehyde (p-DMAB) into 100ml of concentrated phosphoric acid (85%) which was added slowly** to 100ml methanol (99.9%) to achieve a 1:1 solution. The reagent solution was then stored in an amber bottle at room temperature.

For the initial run, I carefully weighed out 200, 100, 50, 25, and 12.5mg samples in five small glass jars and 5ml of boiling water was added to each of them. The jars were then placed into a hot water bath for 15 minutes to extract the alkaloids. Meanwhile, a pipette was used to dispense 3ml of reagent solution to five standard-size 2-dram vials. After 15 minutes, a 1ml syringe was used to draw 1ml of each extract through a cotton ball in order to filter out the powdered mushroom material*** before being added to the reagent vials.

Results: After about 1 hour, a color reaction developed so that there is a clear gradient-scale from vial #1 (200mg) to #2 (100mg) to #3 (50mg). Vials #4 and #5 (25mg and 12.5mg) show little to no evidence of a reaction.



Although further trials are obviously needed in order to "calibrate" the test so that a useful gradient-scale can be devised, these results show that the concept is likely workable.

In order to become a useful test, the next challenges to overcome are (1) to increase the intensity and optimize the visibility of the color-reaction, (2) to establish the upper and lower limits of the color-reaction so that a gradient-scale can be devised, and (3) to establish the most useful amounts of both the reagent and extraction solutions so that samples of average potency will result in a color corresponding to the middle of the gradient-scale.

Regarding (1), there are a number of possible solutions to the problem — for example, the amount of extract added to the reagent could be increased, i.e. using 2 or 3ml rather than 1ml, without needing to use greater amounts of mushroom material. Or alternatively, a more efficient solvent like methanol could be used. Another avenue to explore would be to react the two solutions in a hot-water bath instead of at room-temperature.

As to (2) and (3), simple trial and error should be enough to overcome these problems assuming (1) has first been solved.

And lastly, I'd love to hear from you all if you have any constructive criticism or suggestions about how to proceed from here :smile:

Notes:
*Mazatapec, B+, Huautla, and Treasure Coast
**This is an important step because adding a strong acid to methanol results in an exothermic reaction that produces a lot of heat
***Syringe filters would be ideal, but hey ya gotta use what you got


--------------------
Found Species: Ps. Azurescens, Ps. Cyanescens, Ps. Baeocystis, Ps. Semilanceata, Pan. Cinctulus



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OfflineSpectreOfCommunism
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Re: DIY Psilocybin Content Percentage Detection [Re: SpectreOfCommunism] * 1
    #27754027 - 04/27/22 08:22 PM (1 year, 8 months ago)

Addendum: I want to clarify that the intended goal of this first trial run was not to have a fully functional and useful quantitative test but rather to establish some starting values to work with in subsequent trials and particularly to establish a lower limit of sensitivity for the reagent solution. (Which this has accomplished.)

I also want to add that the whole bill for the materials to do this experiment and many more similar experiments has only been about $75 so far. Ehrlich‘s reagent is available on Amazon for about $30, a liter of concentrated phosphoric acid is available for about $20, and methanol (although it is pretty hard to track down) is only about $5 per gallon. The 2-dram vials I used are about 25$ for a 100-pack. And these materials are enough to do thousands of these types of tests.


--------------------
Found Species: Ps. Azurescens, Ps. Cyanescens, Ps. Baeocystis, Ps. Semilanceata, Pan. Cinctulus



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OfflineSpectreOfCommunism
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Re: DIY Psilocybin Content Percentage Detection [Re: SpectreOfCommunism] * 1
    #27754269 - 04/27/22 09:57 PM (1 year, 8 months ago)

Addendum Part II: The Reckoning:
Tonight I plan to run two more experiments and, in order to isolate variables effectively, I will change only one parameter at a time. For the first run, I will be repeating the procedure except that I will add 2ml (rather than 1ml) out of the 5ml of extract to the reagent solution. My expectation is that the color-reaction will be stronger than in the initial experiment. And for the second run, I will repeat the initial procedure except that I will react the sample mixture in a hot-water bath by bringing water to a boil and then shutting off the burner before nesting the vials in the pan. I expect that this will result in a faster reaction and possibly increase the intensity of the final color.


--------------------
Found Species: Ps. Azurescens, Ps. Cyanescens, Ps. Baeocystis, Ps. Semilanceata, Pan. Cinctulus



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Offlinenektar61S
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Re: DIY Psilocybin Content Percentage Detection [Re: shr00med] * 1
    #27754393 - 04/28/22 12:12 AM (1 year, 8 months ago)

Good ideas here. (except the new guy who thought that developing a DIY testing process is harming some random dude's IP, haha.)

If you want to try to make a color test chart that can be used by others, as A-Rock said, turn off the auto functions on camera. Also should white balance camera, and mention make and model of camera, plus color temp of light. At least what the light is sold as.

Also would be good to include a photo using that camera / settings/ lighting of a standard color checker card like this
https://www.ebay.com/itm/224566675111?hash=item34493786a7:g:jMQAAOSwJFhhEhzw

I'll get some Erlich's when I get paid in a couple weeks. I certainly have some strong clone shroom and some weaker MS shroom I could start by comparing.

I do predict that the price of milligram-accurate lab tests will go down, especially with the Oregon therapeutic protocols requiring testing (potency, plus microbials) of every batch not over 5 pounds of shrooms.

If someone developed an accurate mail-in TLC or other lab test that's 10 dollars instead of 100 dollars, they'd be flooded with customers from day one until forever.


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Edited by nektar61 (05/01/22 12:32 AM)


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OfflineSpectreOfCommunism
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Re: DIY Psilocybin Content Percentage Detection [Re: nektar61] * 2
    #27754400 - 04/28/22 12:29 AM (1 year, 8 months ago)

Quote:

nektar61 said:
Good ideas here. (except the new guy who thought that developing a DIY testing process is harming some random dude's IP, haha. 

If you want to try to make a color test chart that can be used by others, as A-Rock said, turn off the auto functions on camera. Also should white balance camera, and mention make and model of camera, plus color temp of light. At least what the light is sold as.

Also would be good to include a photo using that camera / settings/ lighting of a standard color checker card like this
https://www.ebay.com/itm/224566675111?hash=item34493786a7:g:jMQAAOSwJFhhEhzw




All good suggestions and I fully intend to be rigorous and transparent about camera settings, light, etc once I reach the endgame-stage where I'm devising a gradient-scale for the use of everyone -- I didn't bother with that rigor this time around because this was just the initial stage where I chose a somewhat arbitrary starting point to start gathering data. Once I've trial'd and error'd enough to determine the most useful ratios of reagents, solvents, etc, to position my averaged sample at the center of the colorimetric reaction, then the last phase (hopefully inshallah) will be to take some very scientifically-minded photos lol -- but these photos are just "process" photos to let those following the thread in on what's happening over here

As I write this sentence, I am in the process of observing round #2 and #3 :smile: I'll probably have another round of photos before the night is out

Thanks for your engagement and thoughtful response :smile:


--------------------
Found Species: Ps. Azurescens, Ps. Cyanescens, Ps. Baeocystis, Ps. Semilanceata, Pan. Cinctulus



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Offlinenektar61S
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Re: DIY Psilocybin Content Percentage Detection [Re: SpectreOfCommunism] * 1
    #27754433 - 04/28/22 01:51 AM (1 year, 8 months ago)

Quote:

SpectreOfCommunism said:
...but these photos are just "process" photos to let those following the thread in on what's happening over here

...Thanks for your engagement and thoughtful response :smile:




One suggestion I'd have is maybe double the amount of test shrooms or double regent perhaps. (or both?)

I think brighter results would have more meaning and be easier to discern differences. At quick glance the one on the far left is the only one that looks like much of a reaction in your tests.

Maybe double the amount of DMAB in your reagent but keep the amount of ethanol and HCL same as you've been using?

Does anyone here know if filtering out the powdered mushroom material is needed, is it used in commercial kits? I doubt it's needed in the field tests cops use for LSD, which only indicate presence, and maybe not amount, but would work on a much smaller amount, micrograms of indole compound compared to the milligrams with shrooms.

Thank you for jumping in feet first on this. I was happy to see this thread, I was wondering the same thing recently.


--------------------
-NEW? Start here.


Edited by nektar61 (04/28/22 02:17 AM)


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InvisibleQM33
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Re: DIY Psilocybin Content Percentage Detection [Re: nektar61] * 1
    #27754542 - 04/28/22 05:34 AM (1 year, 8 months ago)

Ya I'm confused as to why you essentially made a tea prior to the test. Imo your pulling out pigmentation that is going to  variabloze it most likely. Seems as though the reactions are even somewhat tea colored..



And actually idk why you would even do that, because what your doing is adding a bunch of water, 33-25% depending on how your looking at it, the mushroom material could have been added powdered..?

This should keep the reagent more colorful, and idk, not a chemistry guy, but adding that much water to a solution may make it generally less effective.

I don't think filtering is necessary.



Lol nektar, double the shrooms or reagent or both?
Do both your right back where you started haha.



And that color card looks like it belongs on the third episode of Barney.

Your probably going to need to see what color is your primary color and see what kind of shades your getting, and probably much more intentionally make your own scale.


I think your onto something.

And why would you need or want elrich for this? I thought the whole point was to make your own reagent for much cheaper and then make your own scale that would be completely different than elrich?


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Re: DIY Psilocybin Content Percentage Detection [Re: QM33] * 1
    #27755098 - 04/28/22 01:11 PM (1 year, 8 months ago)

Quote:

QM33 said:
Ya I'm confused as to why you essentially made a tea prior to the test. Imo your pulling out pigmentation that is going to  variabloze it most likely. Seems as though the reactions are even somewhat tea colored..

And actually idk why you would even do that, because what your doing is adding a bunch of water, 33-25% depending on how your looking at it, the mushroom material could have been added powdered..?




I’m doing so for several reasons — firstly, because we know that psilocybin and it’s relatives are polar and water-soluble and so a water-extraction contains the only thing we’re concerned with testing. Secondly, the DMAB color-reaction is an aqueous reaction and so the alkaloids need to be dissolved one way or another — doing so in a known quantity of a good solvent for a controlled duration is a good approach which is repeatable while also removing >95% of the non-alkaloid content.

And lastly, because the Miraculix kit also works by making an extract of the mushroom powder with boiling water and, since Miraculix is the only example I have of a functioning colorimetric indole-test, that seems like a good place to start.

I don’t think pigments are going to be an issue and I’m not sure how a water-extract would add more of them than adding the powdered mushroom directly as you suggest — why would the extract have more pigment than the thing it was extracted from itself? 

Quote:

QM33 said:
I don't think filtering is necessary.




I think it is. Without filtering, there would be different proportions of rehydrated mushroom-mush to water-extract in every test which would be impossible to know or control. Also, as you already pointed out, this would add a lot of confounding colors to the reagent which would muddy the reaction. Plus, the mushroom material will clog the syringe without the cotton ball.

Quote:

QM33 said:
And why would you need or want elrich for this? I thought the whole point was to make your own reagent for much cheaper and then make your own scale that would be completely different than elrich?




My goal is to design the cheapest, most effective, most accessible DIY colorimetric test possible. And since 100g of DMAB (enough to make about 8 liters of concentrated reagent) only costs as much as an OK bottle of whisky, the cost and effort to synthesize the reagent at home doesn’t seem justifiable


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