|
Hank Hi11
Stranger

Registered: 01/20/22
Posts: 38
Last seen: 2 years, 1 month
|
Pre pour my own petrie dishes plan!
#27678201 - 03/01/22 12:18 PM (2 years, 2 months ago) |
|
|

OK, so I've now worked out it's wise to get my agar game sorted going fwd.
I plan on pouring my agar into my borosilicate petrie's and stacking them inside this quart jar (upside down and screwed very lightly) and autoclaving for the recommended time.
The obvious concerns I have is that these are quite tiny dishes and I'm assuming the sterilisation process might boil them off/make a huge mess (bubbling, if too much in each dish)
....and also condensation.
The plan is to pour them reasonably shallow and let them cool totally in the autoclave before removing.
Anybody with words of caution/advice and can warn me of major drama's to unfold?
Cheers for any and all help.
|
shr00med
Stranger
Registered: 03/10/21
Posts: 29
|
Re: Pre pour my own petrie dishes plan! [Re: Hank Hi11]
#27682013 - 03/04/22 05:52 AM (2 years, 2 months ago) |
|
|
I'd make sure to wrap em up in foil before sterilizing and definitely wait to cool to help with the condensation. Personally, I wouldn't deal with this method unless you're working with a SAB
|
7Suns
Researcher



Registered: 05/11/14
Posts: 765
Loc: not present
Last seen: 1 hour, 47 minutes
|
Re: Pre pour my own petrie dishes plan! [Re: shr00med]
#27693620 - 03/13/22 10:21 AM (2 years, 2 months ago) |
|
|
Why not just pour them In front of a flowhood?
-------------------- Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage 7suns grain sac hack 7suns straw log hack 7suns official OTC antibiotic agar recipe 7suns DIY filter bags 7suns cheap AF flowhood 7suns cold weather fruit chamber
|
fossilshark
DouchebagDonny



Registered: 08/05/20
Posts: 132
Last seen: 3 months, 3 days
|
Re: Pre pour my own petrie dishes plan! [Re: 7Suns] 1
#27694833 - 03/14/22 10:15 AM (2 years, 2 months ago) |
|
|
This is not advanced mycology.
-------------------- LITFA LITFA LITFA
|
Inigo Montoya
Stranger

Registered: 12/20/21
Posts: 10
Last seen: 1 year, 7 months
|
Re: Pre pour my own petrie dishes plan! [Re: fossilshark]
#27694926 - 03/14/22 11:40 AM (2 years, 2 months ago) |
|
|
It's advanced for me! Guess it's subjective lol
|
dyel



Registered: 10/15/21
Posts: 920
Last seen: 2 months, 2 days
|
Re: Pre pour my own petrie dishes plan! [Re: 7Suns]
#27695129 - 03/14/22 02:52 PM (2 years, 2 months ago) |
|
|
check out home mycology on yt, he uses this on regular basis
Quote:
7Suns said: Why not just pour them In front of a flowhood?
I think I should pull out the wallet and get me one and start poring my agar, but I'm sure some people here can't afford one so no-pour will always be a thing
|
7Suns
Researcher



Registered: 05/11/14
Posts: 765
Loc: not present
Last seen: 1 hour, 47 minutes
|
Re: Pre pour my own petrie dishes plan! [Re: dyel]
#27695252 - 03/14/22 04:33 PM (2 years, 2 months ago) |
|
|
Quote:
dyel said: check out home mycology on yt, he uses this on regular basis
Quote:
7Suns said: Why not just pour them In front of a flowhood?
I think I should pull out the wallet and get me one and start poring my agar, but I'm sure some people here can't afford one so no-pour will always be a thing
Look at the links in my bio, I built one for $40 and it kicks ass, I haven’t had any contamination in over a year
-------------------- Following the lost souls as they transcend the seven dimensions, through playful dancing, like wandering shadows in the heat of a mirage 7suns grain sac hack 7suns straw log hack 7suns official OTC antibiotic agar recipe 7suns DIY filter bags 7suns cheap AF flowhood 7suns cold weather fruit chamber
|
|