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InvisibleShroomintune
Gentleman Farmer
I'm a teapot User Gallery


Registered: 06/04/21
Posts: 267
Loc: Outside of the Asylum
Re: Post Your New Tools Of The Trade. [Re: milkboy]
    #27677398 - 02/28/22 08:19 PM (2 years, 2 months ago)

Quote:

milkboy said:
I have a stupid question... I have an enail that I dont use anymore, could I use that as a hommade induction sterilizer? Id move away from butane if thats the case, coil can heat to around 1000°f.




Nope, all enails I've seen (and the I'm about to go hit) are resistance heaters that happen to be bent in the shape of a coil, not induction heaters.

In an induction heater, the coil itself does not get hot, it "induces" an electrical current in the object being heated, which heats only the object in the center of the coil up.  That's why they only work on metal. 

If you have an induction stove, you can pull a pot of boiling water off, put your hand on the "burner", and the burner itself will be cool.

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Invisiblemilkboy
Child
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Registered: 05/01/21
Posts: 2,389
Re: Post Your New Tools Of The Trade. [Re: Shroomintune]
    #27677422 - 02/28/22 08:31 PM (2 years, 2 months ago)

Damn... thanks for explaining. I figured it was silly but wanted to ask regardless. Guess it's the torch for now.


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InvisibleShroomintune
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Registered: 06/04/21
Posts: 267
Loc: Outside of the Asylum
Re: Post Your New Tools Of The Trade. [Re: Shroomintune]
    #27677425 - 02/28/22 08:32 PM (2 years, 2 months ago)

After some googling, it looks like there is ONE company that sells a true induction e-nail.  https://www.getispire.com/

But I also found this guy's DIY induction coil e-nail rig, which looks like it could be the basis for a homebrew induction sterilizer.

https://fuckcombustion.com/threads/induction-heated-e-nail-with-arduino-closed-loop-temp-control.22605/

Also, quarter shrinking.  http://www.capturedlightning.com/frames/shrinkergallery.html

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Invisiblesandman420
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Registered: 06/17/04
Posts: 5,384
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Re: Post Your New Tools Of The Trade. [Re: Shroomintune]
    #27677437 - 02/28/22 08:37 PM (2 years, 2 months ago)

Man I gotta step my nail/banger game up


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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OfflineEnjoil
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Registered: 10/29/20
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Re: Post Your New Tools Of The Trade. [Re: milkboy]
    #27677533 - 02/28/22 10:03 PM (2 years, 2 months ago)

I was joking with you bro. Yeah they can get stupid expensive… props on making your own

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InvisibleSwabMarley
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Re: Post Your New Tools Of The Trade. [Re: Enjoil] * 3
    #27681995 - 03/04/22 05:30 AM (2 years, 2 months ago)

Gunna see what this distilled sterile water culture storage is all about…

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OfflineHindsight
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Re: Post Your New Tools Of The Trade. [Re: SwabMarley]
    #27682087 - 03/04/22 06:54 AM (2 years, 2 months ago)

I just loaded up a bunch of them clone cultures and will see what happens. I PCd them in a polypropelene vial holder which worked out really nicely. I got three of those holders - one for active use on the flow bench, one to hold the vials in storage, and one to PC them in. I also like having a sterilized vial opened up in front of the hood while I'm knocking up plates - I will dip my loop into it before rubbing the spore print which helps transfer spores. The residual water on the print is minimal and dries in a minute or two. All in all, really liking these things so far. Only issue I have found is that I ordered the vials twice from the same supplier inside of one week and got two different styles. One style has a black rubber gasket in the lid and the other style has a built-in tapered sealing area like a GL45 media bottle cap - I strongly prefer the latter because the o-rings can dislodge while screwing in place and I don't trust their sealing. I wish I could get a guarantee on which style I will receive when I order.

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InvisibleSwabMarley
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Re: Post Your New Tools Of The Trade. [Re: Hindsight]
    #27682089 - 03/04/22 06:58 AM (2 years, 2 months ago)

Nice. Yeah these have a sort of skirt on the bottom so they can stand up. Pretty fuckin cheap for 50. All I can do is give these a go and see how it turns out. I only recently slanted everything a few weeks ago anyways so no worries if it doesn’t! Does kinda seem the way forward though.

@ Hindsight Do you just drop a wedge in or do you use the myc water scrape method?

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OfflineHindsight
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Re: Post Your New Tools Of The Trade. [Re: SwabMarley] * 1
    #27682099 - 03/04/22 07:11 AM (2 years, 2 months ago)

Never heard of the mycelium water scrape.... I assume it means pouring some distilled water on top of a colonized plate, scraping a bit, and then dropping some of that water into the tube? I guess the intent there being to introduce less (or no) nutrients into the vial? I didn't do that - just put a very small chunk of colonized agar in there. I figure that the mycelium will consume the nutrients out of it and then go to sleep, or will run out of oxygen first and go to sleep. Either way, sleep. That's the theory anyway. I hope it works because I get so tired of transferring my clone plate cultures to new plates every month to keep them alive and I've had bad luck with slants in the fridge, and even slants in general. A lot of my cultures won't grow on a slant - they don't like the wood for some strange reason. I need to try new wood (instead of the common Popsicle stick) but I'm hoping these vials work and I won't have to. I also find that of the cultures that do take to slants, they never grow right after I take them out. Like I had two different APE cultures that made nice fruits before and after 6 months in the fridge, they still grew immediately and vigorously on agar, grain, and sub, but refused to fruit, and I tried it in about a dozen different tubs. Primordia would form and right before it turned into pins, it would simply stay in that state..... forever. Left some of them like that for two months! Just sat there.

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InvisibleSwabMarley
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Re: Post Your New Tools Of The Trade. [Re: Hindsight] * 1
    #27682109 - 03/04/22 07:18 AM (2 years, 2 months ago)

Shit man, that sounds like a massive bummer! I’ve got an APE clone slanted that I’m a bit nervous about now!

Yeah the water scrape thingy is outlined Here

I think an agar wedge is more than suffice though and will be for sure the way I go.

Edit: here’s the reference in the above post just to save all the reading:

Quote:

*** About six milliliters of sterile distilled water is pipetted aseptically into a freshly growing culture. The fragments of hyphae are dislodged by lightly scraping the aerial growth with the same pipette, and the resulting suspension is withdrawn and transferred to a sterile glass vial. Put plenty of inoculum into each vial to insure success.



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InvisibleSwabMarley
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Re: Post Your New Tools Of The Trade. [Re: SwabMarley] * 1
    #27682265 - 03/04/22 09:52 AM (2 years, 2 months ago)

18 more borosilicate plates 90X15!

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Offlineaosod
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Re: Post Your New Tools Of The Trade. [Re: SwabMarley]
    #27682793 - 03/04/22 04:31 PM (2 years, 2 months ago)

Quote:

Myc Hunt said:
18 more borosilicate plates 90X15!




Man im high and this picture of these plates just tickle my brain the right way :rasta::stoned:

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InvisibleAphexPin
Strange

Registered: 02/22/22
Posts: 464
Re: Post Your New Tools Of The Trade. [Re: SwabMarley]
    #27682958 - 03/04/22 06:33 PM (2 years, 2 months ago)

Quote:

Myc Hunt said:
Gunna see what this distilled sterile water culture storage is all about…




I'd like to do some long term trials with pouring an LI into these, storing at room temp, and retrieving with a sterile swab.

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InvisibleSwabMarley
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Re: Post Your New Tools Of The Trade. [Re: AphexPin]
    #27683285 - 03/05/22 12:39 AM (2 years, 2 months ago)

Quote:

AphexPin said:
Quote:

Myc Hunt said:
Gunna see what this distilled sterile water culture storage is all about…




I'd like to do some long term trials with pouring an LI into these, storing at room temp, and retrieving with a sterile swab.



Sounds like that may work, I think the key is zero nutrients. I say go for it!

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InvisibleModularMind
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Re: Post Your New Tools Of The Trade. [Re: SwabMarley]
    #27684141 - 03/05/22 05:08 PM (2 years, 2 months ago)

The kind of everything, that’s available for two day shipping, in these endeavors is gladdening.

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OfflineHindsight
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Re: Post Your New Tools Of The Trade. [Re: ModularMind]
    #27685203 - 03/06/22 02:58 PM (2 years, 2 months ago)

For those of you using a flow hood, what do you guys use to hold your tools (loops, xacto knife, scalpels, etc) while working in front of the hood? I really wanted to attach a magnet on the right side frame bezel of the hood and then put my tool on it at a ~45 degree angle so most of the tool is in the air stream. That will work for my scalpel but several of my other tools are aluminum handled (like xacto knife handles which I use for my loop) so that won't work. I can't seem to find any type of friction clips/clamps either. For now I have a block of wood with some holes drilled in it and I set the handles in the holes with the blades pointing up but I don't really like that solution.

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InvisibleTedsDead
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Re: Post Your New Tools Of The Trade. [Re: Hindsight] * 1
    #27685246 - 03/06/22 03:41 PM (2 years, 2 months ago)

I set mine on the table.  Give em a wipe between uses with an iso rag between uses


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weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396

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OfflineHindsight
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Re: Post Your New Tools Of The Trade. [Re: TedsDead]
    #27685279 - 03/06/22 04:04 PM (2 years, 2 months ago)

Reason I like a holder is to keep them from touching any surface and also to keep them up in the air stream to cool down.

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OfflineLogicaL ChaosM
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Re: Post Your New Tools Of The Trade. [Re: Hindsight] * 1
    #27685307 - 03/06/22 04:33 PM (2 years, 2 months ago)

Just got this Craftsman Nail Set for making syringe holes for PF Jars. I used the smallest (pictured left) nail yesterday, works perfect. Couple light hammer hits and perfect small, round hole :yesnod:



--------------------
"What you must understand is that your physical dimension affects everyone in the higher dimensions as well. All things are interconnected. All things are One. Therefore, if one dimension is broken or out of balance, then all other dimensions will experience repercussions." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin

PROJECT BLUE BOOK ANALYSIS! (312 pages!) | Psychedelics & UFOs | Ready to Contact UFOs? | The Source on Mushrooms:shroomeryhead:| Trippy Gematrix | Dj TeknoLogical | Fentanyl Test Kits R.I.P. Big Worm :tombstone: || The Start of the Ascension Process was 2020. Welcome to the Next Great Era of Earth 🌎🌍🌏                                                         
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Invisiblesandman420
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Re: Post Your New Tools Of The Trade. [Re: LogicaL Chaos] * 3
    #27685319 - 03/06/22 04:46 PM (2 years, 2 months ago)

2/32" so nobody gets confused (everybody gets confused) :facepalm3:


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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